태안해안국립공원 학암포 지역의 조류 현황

강화연(Hwa-Yeon Kang),서슬기(Seul-Gi Seo),박세영(Se-Yeong Park),이지용(Ji-Yong Lee),권영수(Young-Soo Kwon) 국립공원연구원 2015 국립공원연구지 Vol.6 No.4

서해안 육상지역을 통과하는 조류의 이동생태를 파악하기 위해, 2014년 7월부터 2015년 6월까지 태안해안국립공원 학암포 지역의 조류상을 조사하였다. 조사 결과 관찰된 조류는 총 14목 30과 88종 23,230개체이며, 평균 조사일당 159.7개체가 관찰되었다. 전체 우점종은 괭이갈매기 Larus crassirostris 21.1%, 되새 Fringilla montifringilla 18.5%, 박새 Parus major 12.7%, 붉은머리오목눈이 Paradoxornis webbianus 8.2%, 노랑턱멧새 Emberiza elegans 5.8% 순이었다. 이동시기인 5월과 10월에는 각각 56종, 조사일당 평균 310.5개체로 가장 많은 종과 개체수가 기록되었다. 종과 개체수가 가장 적은 시기는 여름철인 8월로, 기록된 종 수는 24종이며 조사일당 평균 95개체가 관찰되었다. 종풍부도(R ) 지수는 가장 많은 종이 관찰된 5월에 가장 높았으나, 종다양도(H )와 종균등도(E )는 오히려 이동시기에 낮은 편으로 나타났으며, 이는 일부 상위 우점종의 개체수가 월등히 증가했기 때문인 것으로 판단된다. 환경부, 문화재청, IUCN 등이 지정한 멸종위기 및 법정보호종은 총 9종이 관찰되었다. From July 2014 to Jun 2015, bird survey was conducted in Hakampo, Taeanhaean National Park. A total of 23,230 and 159.7 per day of birds representing 14 orders, 30 families, 88 species were observed. The most abundant species were the Black-tailed Gull Larus crassirostris (21.1%), the Brambling Fringilla montifringilla (18.5%), the Great Tit Parus major (12.7%), the Vinous-throated Parrotbill Paradoxornis webbianus (8.2%), Yellow-throated Bunting Emberiza elegans (5.8%). The number of species and individual was the highest in May and October, respectively, as 56 species and 310.5 individual per day were recorded. According to Korean Ministry of Environment, Cultural Herigage Adinistration of Korea and IUCN Red List, a total of 9 protected species were recorded during the survey.

Distribution of the Fairy Pitta (Pitta nympha) in the South Korea: A focus on protected areas

Gil-Pyo Hong(홍길표),Jae-Hoon Kim(김재훈),Seul-Gi Seo(서슬기),So-Yeon Cho(조소연),Bo-Yeon Hwang(황보연),Jong-Hyun Park(박종현),Seung-Yeon Lee(이승연),Ga-Young Kim(김가영),Wan-Hee Nam(남완희),Ha-Cheol Sung(성하철) 한국조류학회 2021 한국조류학회지 Vol.28 No.2

국내 팔색조(Pitta nympha) 분포 현황을 알아보고자 전국 국립공원 등 보호지역 중심으로 총 19개 지역을 조사하였고, 전문가 네트워크를 통해 자료를 보강하였다. 전국 19개 지역을 조사한 결과 북한산, 지리산 등 내륙지역 7개 국립공원에서는 팔색조가 확인되지 않았고, 경남 거제, 남해, 해남, 장흥 고흥 등 남해안과 인접한 12개 지역에서 팔색조 서식이 확인되었다. 또한 전문가 네트워크를 통해 수집한 팔색조 출현 위치 정보 82건을 분석한 결과, 전국 8개 광역지자체 모두 팔색조 서식이 확인되었고, 내륙지역(26.8%)보다 해안지역(73.2%)에서 더 많이 출현하였다. 연구 결과를 종합해보면, 우리나라의 팔색조는 주로 제주도와 남해안 지역을 중심으로 많이 분포하며, 그 보다 적은 개체수가 내륙 지역에 넓게 분포하고 있는 것으로 나타났다. The present study investigated the current distribution of Fairy Pitta (Pitta nympha) in South Korea. A total of 19 study areas, including protected areas such as national and provincial parks in South Korea were surveyed, and additional data obtained from an expert network. According to the results, Fairy Pitta was absent in seven inland national parks, including Mt. Bukhan and Chiri parks. Fairy Pitta inhabited 12 areas near the southern coast of South Korea, including Geoje, Namhae, Haenam, Jangheung, Goheung, and Gyeongnam Province. The analysis results of 82 datasets obtained from experts with regard to the locations where Fairy Pitta have been spotted showed that the bird inhabits all the eight metropolitan regions in South Korea, and is more frequently spotted in coastal areas (73.2%) than in inland areas (26.8%). The findings demonstrate that Fairy Pitta is mainly distributed in Jeju Island and the southern coast of South Korea, and is distributed in inland areas at relatively lower numbers.

Evaluation of Barley and Wheat Germplasm for Resistance to Head Blight and Mycotoxin Production by Fusarium asiaticum and F. graminearum

Seul Gi Baek(Seul Gi Baek),Jin Ju Park(Jin Ju Park),Sosoo Kim(Sosoo Kim),Mi-Jeong Lee(Mi-Jeong Lee),Ji-Seon Paek(Ji-Seon Paek),Jangnam Choi(Jangnam Choi),Ja Yeong Jang(Ja Yeong Jang),Jeomsoon Kim(Jeom 한국식물병리학회 2022 Plant Pathology Journal Vol.38 No.6

Fusarium head blight (FHB) is one of the most serious diseases in barley and wheat, as it is usually accompanied by the production of harmful mycotoxins in the grains. To identify FHB-resistant breeding resources, we evaluated 60 elite germplasm accessions of barley (24) and wheat (36) for FHB and mycotoxin accumulation. Assessments were performed in a greenhouse and five heads per accession were inoculated with both Fusarium asiaticum (Fa73, nivalenol producer) and F. graminearum (Fg39, deoxynivalenol producer) strains. While the accessions varied in disease severity and mycotoxin production, four wheat and one barley showed <20% FHB severity repeatedly by both strains. Mycotoxin levels in these accessions ranged up to 3.9 mg/ kg. FHB severity was generally higher in barley than in wheat, and Fa73 was more aggressive in both crops than Fg39. Fg39 itself, however, was more aggressive toward wheat and produced more mycotoxin in wheat than in barley. FHB severity by Fa73 and Fg39 were moderately correlated in both crops (r = 0.57/0.60 in barley and 0.42/0.58 in wheat). FHB severity and toxin production were also correlated in both crops, with a stronger correlation for Fa73 (r = 0.42/0.82 in barley, 0.70 in wheat) than for Fg39.

Effect of Ovarian Extract on Oocyte Maturation and Early Embryonic Development in Pigs

Seul-Gi Yang(Seul-Gi Yang),Jae-Hun Choi(Jae-Hun Choi),Young-Seo Jo(Young-Seo Jo),Ye-Won Kim(Ye-Won Kim),Dong-Mok Lee(Dong-Mok Lee),Hyo-Jin Park(Hyo-Jin Park),Deog-Bon Koo(Deog-Bon Koo) 한국동물보건학회 2022 한국동물보건학회지 Vol.1 No.2

Various factors in the ovary are known to regulate oocyte maturation and hormone secretory functions; however, the effect of ovarian extract (OE) on oocyte maturation and embryonic development in pigs remains unknown. In this study, we first evaluated whether OE supplementation in the in vitro maturation (IVM) medium alters the oocyte maturation capacity by affecting glucose/amino acid metabolites, meiotic maturation, cumulus cell (CC) expansion, and antioxidants. Various OE concentrations (50, 100, 200, 500, and 5000 μg/mL) were included in the IVM medium. Only the oocytes treated with 100 μg/mL OE exhibited an improved meiotic maturation rate when compared with that of the other groups (non-treated group, 78.6 ± 3.0% vs. 100 μg/mL OE-treated group, 81.6 ± 4.3%); however, the difference was not significant. To observe the changes in glucose and amino acid metabolism in the OE-treated oocytes, we measured the amounts of diverse constituents (glucose, lactate, glutamine, and ammonia) in the IVM medium containing OE. Lactate and ammonia levels in the OE-treated group after 44 h of IVM were higher (p < 0.01) than those in the non-treated group. In addition, the expression of the CC expansion factors (Has2 and Tnfaip6) significantly increased (p < 0.05), whereas the mRNA expression levels of antioxidative enzymes (Sod1, Cat, and Gpx1) significantly diminished (p < 0.05) in the OE-treated group. Moreover, mature oocytes treated with 100 μg/mL OE demonstrated increased subsequent embryonic development rates after 144 h of IVM. Thus, the addition of OE in IVM mediums may improve oocyte maturation capacity which could enhance antioxidative enzyme activation, energy metabolism, and expression of the CC expansion factors in porcine oocytes.

A Case of Midgut Volvulus with Gastric Perforation and Periveintricular Leukomalacia in a Term Infant

Park, Seul Gi,Hwang, Jong Hee 대한신생아학회 2021 Neonatal medicine Vol.28 No.1

Intestinal malrotation with midgut volvulus (MV) is a life-threatening surgical emergency. Most events of MV occur in the neonatal period with bilious vomiting, abdominal distension, feeding intolerance, and bloody stools. Neonatal gastric perforation (GP) is a rare and life-threatening condition associated with high mortality. It occurs either in an idiopathic form or in association with gastrointestinal anomalies such as duodenal atresia and MV. The pathogenesis of both MV and GP is related to ischemic change and inflammatory response. MV and GP can lead to morbidities such as sepsis, intestinal ischemia, and organ failure, but not neurologic problems. We herein report the case of a term infant at 5 days after birth, with MV accompanied by GP, who developed periventricular leukomalacia.

Placental defects in Asb2 knockout mice is associated with heart defects and early embryonic lethality

Seul Gi Park,Eun-kyoung Kim,Hyoung-Chin Kim,Won-Kee Yoon,Ki-Hoan Nam,Sang-Yoon Nam 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

Asb2, ankyrin repeat and SOCS box protein 2 form an E3 ubiquitin ligase complex. Asb2 ubiquitin ligase activity drives the degradation of filamins, which have essential functions in humans [1, 2]. The placenta is a temporary organ that is formed during pregnancy, and normal placentation is important for survival and growth of the fetus [3]. Recent studies have shown that approximately 25-30% of knockout (KO) mice have non-viable offspring, and 68% of knockout lines exhibit placental dysmorphologies [4]. There are very few studies on Asb2, with insufficient researches on its role in placental development. We generated Asb2 knockout mice and investigated Asb2 expression during organogenesis, and identified its role in early embryonic and placental development. The external morphology of KO embryos revealed abnormal phenotypes including growth retardation, peri-cardial effusion, pale color, and especially heart beat defect from E 9.5. Furthermore, Asb2 expression was observed in the heart from E 9.5, indicating that it is specifically expressed during early heart formation and defects, result in embryonic lethality. Histological analysis of E 10.5 KO mouse heart showed malformations such as failure of chamber formation, reduction in trabeculated myocardium length, absence of mesenchymal cells, and destruction of myocardial wall. Moreover, the histological observation of the placenta for Asb2-deficient mice showed abnormal phenotypes including small labyrinth and reduced vascular complexity, indicating that failure to establish mature circulatory pattern affects the embryonic development and associated with the early mortality. Collectively, our results demonstrate that Asb2 knockout mice have placental defects, and failure to form a normal cardiac septum, and thereby result in embryonic lethality in utero at around E 9.5. References [1] Babon, J.J., et al., The SOCS box domain of SOCS3: structure and interaction with the elonginBC-cullin5 ubiquitin ligase. J Mol Biol, 2008. 381(4): p. 928-40.[2] Heuze, M.L., et al., ASB2 is an Elongin BC-interacting protein that can assemble with Cullin 5 and Rbx1 to reconstitute an E3 ubiquitin ligase complex. J Biol Chem, 2005. 280(7): p. 5468-74.[3] Cross, J.C., D.G. Simmons, and E.D. Watson, Chorioallantoic morphogenesis and formation of the placental villous tree. Ann N Y Acad Sci, 2003. 995: p. 84-93.[4] Rossant, J. and J.C. Cross, Placental development: lessons from mouse mutants. Nat Rev Genet, 2001. 2(7): p. 538-48.

Effects of zinc supplementation on catch-up growth in children with failure to thrive

Seul-Gi Park,Ha-Neul Choi,Hye-Ran Yang,Jung-Eun Yim 한국영양학회 2017 Nutrition Research and Practice Vol.11 No.6

BACKGROUND/OBJECTIVES: Although globally the numbers of children diagnosed with failure to thrive (FTT) have decreased, FTT is still a serious pediatric problem. We aimed to investigate the effects of zinc supplementation for 6 months on growth parameters of infants and children with FTT. SUBJECTS/METHODS: In this retrospective study, of the 114 participants aged between 4 months and 6 years, 89 were included in the zinc supplementation group and were provided with nutrition counseling plus an oral zinc supplement for 6 months. The caregivers of the 25 participants in the control group received nutrition counseling alone. Medical data of these children, including sex, age, height, weight, serum zinc level, and serum insulin-like growth factor 1 (IGF1) level were analyzed. RESULTS: Zinc supplementation for 6 months increased weight-for-age Z-score and serum zinc levels (5.5%) in the zinc supplementation group of underweight category children. As for stunting category, height-for-age Z-score of the participants in the zinc supplementation group increased when compared with the baseline, and serum zinc levels increased in the normal or mild stunting group. Serum IGF1 levels did not change significantly in any group. Thus, zinc supplementation was more effective in children in the underweight category than those in the stunted category; this effect differed according to the degree of the FTT. CONCLUSION: These findings suggest that zinc supplementation may have beneficial effects for growth of infants and children with FTT, and zinc supplementation would be required according to degree of FTT.

Capsaicin protects spermatogenic cell death caused by scrotal hyperthermia through its antioxidative and anti-apoptotic activities

Seul Gi Park,Jung Min Yon,Chun mei Lin,Lee Wha Gwon,Jong Geol Lee,In Jeoung Baek,Beom Jun Lee,Young Won Yun,Sang Yoon Nam 대한수의학회 2016 대한수의학회 학술대회발표집 Vol.2016 No.-

Reduction of mitochondria derived superoxide by Mito-TEMPO improves the porcine oocyte maturation

Seul-Gi Yang,Hyo-Jin Park,Jin-Woo Kim,Min-Ji Kim,Ho-Guen Jegal,In-Su Kim,Min-Young Guk,Sun-Mi Park,Ji-Eun Lee,Deog-Bon Koo,Joung Jun Park 한국수정란이식학회 2018 한국수정란이식학회 학술대회 Vol.2018 No.11

In general, the shape of cumulus-oocyte-complexes (COCs) at germinal vesicle (GV) stage is important roles on meiotic maturation of porcine oocyte during in vitro maturation (IVM). Then, mitochondria produce reactive oxygen species (ROS) such as superoxide from electron transport system during oocyte maturation. ROS levels on oocytes are regulated by various antioxidant enzymes in cumulus cells (CCs). However, the effect of mitochondria derived superoxide production from CCs during IVM of porcine oocyte has not been reported. Firstly, we divided groups according to large number of CCs (Grade 1: G1) and small number of CCs (Grade 2: G2). Then, we counted cumulus cells of G1 and G2 oocyte by using haemocytometer. The oocyte maturation rate was significant decreased (p < 0.05) in G2 oocytes than that of G1 oocytes. We measured mitochondria derived superoxide in G1 and G2 COCs by using Mito-SOX staining. Mitochondrial superoxide was higher in G2 COCs than G1 COCs. Then, the mRNA expression levels of antioxidant enzymes (SOD1, SOD2 and PRDX3) in G2 COCs were decreased compared to G1 COCs. To reduce mitochondria derived superoxide, we used Mito-TEMPO as mitochondrial superoxide scavenger. Oocyte maturation rates in both G1 and G2 groups treated with Mito-TEMPO were increased than that of non-treated groups. Mitochondrial superoxide was lower in G1 and G2 groups treated with Mito-TEMPO than that of non-treatment groups. The mRNA expression levels of antioxidant enzymes in G1 and G2 COCs treated with Mito-TEMPO were increased compared to non-treated groups. Based on these findings, we suggest that reduction of mitochondria derived superoxide by Mito-TEMPO assists maturation competence in porcine oocytes.

Regulation of the Endoplasmic Reticulum Stress by BIP/GRP78 is involved in Meiotic Maturation of Porcine Oocytes In Vitro

Park, Hyo-Jin,Park, Jae-Young,Kim, Jin-Woo,Yang, Seul-Gi,Jung, Jae-Min,Kim, Min-Ji,Park, Joung Jun,Koo, Deog-Bon The Korean Society of Developmental Biology 2017 발생과 생식 Vol.21 No.4

In the present study, we investigated the role of binding immunoglobulin protein/glucose-regulated protein, 78-kDa (BIP/GRP78)-regulated endoplasmic reticulum (ER)-stress on meiotic maturation and cumulus cells expansion in porcine cumulus-oocyte complexes (COCs). Previously, it has been demonstrated that unfolded protein response (UPR)-related genes, such as molecules involved in ER-stress defense mechanisms, were expressed in matured oocytes and cumulus cells during in vitro maturation (IVM) of porcine oocytes. However, BIP/GRP78-mediated regulation of ER stress in porcine oocytes has not been reported. Firstly, we observed the effects of knockdown of BIP/GRP78 (an UPR initiation marker) using porcine-specific siRNAs (#909, #693, and #1570) on oocyte maturation. Among all siRNAs, siRNA #693 significantly reduced the protein levels of UPR marker proteins (BIP/GRP78, ATF4, and P90ATF6) in porcine COCs observed by Western blotting and immunofluorescence analysis. We also observed that the reduction of BIP/GRP78 levels by siRNA#693 significantly inhibited the meiotic maturation of oocytes (siRNA #693: $32.5{\pm}10.1%$ vs control: $77.8{\pm}5.3%$). In addition, we also checked the effect of ER-stress inhibitors, tauroursodeoxycholic acid (TUDCA, $200{\mu}M$) and melatonin ($0.1{\mu}M$), in BIP/GRP78-knockdown oocytes. TUDCA and melatonin treatment could restore the expression levels of ER-stress marker proteins (BIP/GRP78, $p-eIF2{\alpha}$, $eIF2{\alpha}$, ATF4, and P90ATF6) in siRNA #693-transfected matured COCs. In conclusion, these results demonstrated that BIP/GRP78-mediated regulation of UPR signaling and ER stress plays an important role in in vitro maturation of porcine oocytes.