Acinetobacter sp. BE-254에 의한 유화제의 생산

김순한,임이종,최경숙,정영기,장경립,이태호 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

Bioemulsifier를 생산하는 미생물을 토양으로부터 screening하였다. 그 중에서 유화활성과 유화안정성에서 가장 우수한 BE-254 균주를 순수분리하여 동정한 결과, Acinetobacter속으로 판명되었다. Bioemulsifier 생산을 위한 최적배지 조성은 n-hexadecane 4%, NaNO_3 0.2%, KH_2PO_4 0.05%, MgSO_4·7H_2O 0.05%, CaCl_2 0.01%, yeast extract 0.01%이였으며, 최적온도와 pH는 각각 30℃와 7.0이였다. 이러한 조건에서 500㎖용 shaking flask에 최적배지 50㎖를 넣어 배양했을 경우 대수증식기 말기인 5일째 균의 증식과 유화활성이 가장 높게 나타남에 따라 Acinetobacter sp. BE-254에 의한 bioemulsifier의 생산은 균의 생육과 밀접한 관련이 있는 것으로 판단되었다. 유화기질로서 hydrocarbon류, edible oil류, 그리고 petroleum fraction등에 작용시켰을 경우 이들 물질에 대해서 비교적 높은 활성을 나타내어 유화제 로서의 우수성을 시사 하였다. The strain producing bioemulsifier was isolated from soil samples. The isolated strain was identified as the genus Acinetobacter through its morphological, cultural and physiological characteristics. The highest emulsification activity and stability by Acinetobacter sp. BE-254 was observed after 5 days of cultivation in the culture medium containing n-hexadecane 4%, NaNO_3 0.2%, KH_2PO_4 0.01%, MgSO_4·7H_2O 0.01%, CaCl_2 0.01% and yeast extract 0.01%. The optimum pH and temperature for bioemulsifier production were pH 7.0 and 30℃, respectively. Furthermore the most of bioemulsifier was produced during the exponential growth phase, and this suggested that the bioemulsifier production was growth-associated. The bioemulsifier showed good emulsification activity on various emulsifying substrates such as hydrocarbons, edible oils, and petroeum fractions.

발 아치의 기능적, 임상적, 실용적 연구 관점

김승재,박경호,김용이 한국운동역학회 1999 한국운동역학회지 Vol.9 No.1

Mammals, including human, store much of energy needed to run with elastic structures of foot and leg. The important structures to store the elastic strain energy in human body are Achilles tendon and foot arch, however, the foot arch is unique structure in human without the other mammals between the two. Therefore, the purposes of this study were firstly to review comprehensively the literatures on the studies on the functional roles, clinical results and practical applications of the foot arch, and secondly to propose the future directions of research on the foot arch based on the review. To do so, this review are described to divide into four categories; 1) deformity of the foot arch and injury,, 2) ligament, muscle and tendon to form the foot arch, 3) determination of arch height, 4) orthotics and sports shoe for the foot arch types. As a result of reviewing comprehensively the research on the foot arch on functional, clinical and practical aspects, abnormal feet such an low-arched foot and high-arched foot are shown to have significant relationship with injuries during walking and running. To overcome this it is required to understand the muscles and ligaments to form the foot arch throughly, and to be able to reconstruct the abnormal foot arch clinically based on the information. In the practical aspects, it is expected to perform healthy sports activities without any injury with making the proper orthotics and sports shoe according to the foot arch types by determining the arch height at dynamic situation rather than static situation. For the future directions it will need to develop an integrated model to explain both static and dynamic characteristics of the foot and to make theoretical study throughly on the foot arch using the model. Based on the integrated model proposed it will need to develop clinical treatment to prevent walking injury and to make practical study on the development of individualized orthotics and sports shoe according to foot characteristics.

수중액중의 중금속에 대한 녹차의 흡착성질

권이열,김미경,이동섭,홍순영,전혜옥 漢陽大學校 環境科學硏究所 1992 環境科學論文集 Vol.13 No.-

본 연구는 수용액중의 중금속에 대하여 녹차를 이용하여 흡착능을 고찰하였다. 실험에 사용된 녹차는 시판되는 "태평양화학 설록차(2번차)"를 40∼60mesh로 만들어 사용하였다. 각 중금속이온을 함유한 시료용액에 녹차를 첨가하여 흔든 다음, 원심분리해서 상등액중의 각 이온농도를 원자흡광광도계로 측정했다. 그 결과, 각 조건에서 녹차에 의한 중금속의 최대흡착율은 Cu(Ⅱ)는 65∼78%, Cd(Ⅱ)는 78∼82%, Pb(Ⅱ)는 82∼92%였으며, 반응초기 10분간에 최대흡착율의 90%가 일어났다. The adsorptivities of the green-tea particles on Pb(Ⅱ), Cd(Ⅱ) and Cu(Ⅱ) ions were examined by measurements of the adsorption percentage under various conditions, and the method to collect and remove the metallic ion in aqueous solutions were described. Each of 100ml sample solution of Pb(Ⅱ),Cd(Ⅱ) and Cu(Ⅱ) ions mixed with 2g of the green-tea under stirring for minutes. The solutions were then centrifuged, and the concentrations of Pb(Ⅱ), Cd(Ⅱ), and Cu(Ⅱ) ions in the supernatant solution were determined by the atomic absorption spectrometer. As the results, the adsorption equilibrium of Pb(Ⅱ), Cd(Ⅱ) and Cu(Ⅱ) ions were reached to equilibrium by shaking for about 30∼40minutes. Then adsorptivities were 84%, 79% and 65% respectively.

수중에서 감잎의 Cu(Ⅱ), Pb(Ⅱ) 이온에 대한 흡착능

권이열,전미희,김미경 漢陽大學校 環境科學硏究所 1993 環境科學論文集 Vol.14 No.-

본 연구는 감잎을 이용하여 수용액중의 중금속이온 Pb(II), Cu(II)의 흡착능을 고찰하였다. 시료인 감잎은 승우바이오(주)의 감잎차를 50-60mesh로 만들어 사용하였다. 각 중금속이온을 함유한 시료용액에 감잎을 첨가하여 항온조에서 교반시킨 다음, 상등액중의 각 중금속이온 농도를 원자흡광광도계로 측정하였다. 그 결과, 각 조건에서 감잎에 의한 중금속의 최대흡착율은 Cu(II)는 46.3%, Pb(II)는 89.8%였으며, 흡착평형도달시간은 15분에서 각각 36.1%, 79.8%의 흡착율을 보였다. In an attempt to prove adsorption ability of heavy metallic ion(Pb(II), Cu(II) in aqueous solution, this study is made by experimental batch test making use of persimmon leaves. The solutions were then centrifuged and the concentrations of Pb(II), Cu(II) ions in the supernatant solution were dertermined by the atomic adsorption spectrophotometer. As the results, the rate of heavy metallic abolition of persimmon leaves according to the adsorption time show 36.1% in Cu(II) and 79.6% in Pb(II) resulting in Pb(II)'s better than Cu(II) about two times. Concerning the heavy metallic concentration, the maximum removal efficiency of Cu(II) and Pb(II) show 46.3% and 89.8% respectively in 40ppm.

Acinetobacter sp. BE-254에 의한 유화제의 생산

최경숙,장경립,임이종,김순한,정영기,이태호 동의대학교 기초과학연구소 1997 基礎科學硏究論文集 Vol.7 No.1

The strain producing bioemulsifier was isolated from soil samples. The isolated strain was identified as the genus Acinetobacter through its morphological, cultural and physiological characteristics. The highest emulsification activity and stability by Acinetobacter sp. BE-254 was observed after 5 days of cultivation in the culture medium containing n-hexadecane 4%, NaNO₃0.2%, KH₂PO₄0.01%, MgSO₄·7H₂O 0.01%, CaCl₂0.01%, and yeast extract 0.01%. The optimum pH and temperature for bioemulsifier production were pH 7.0 and 30℃, respectively. Furthermore the most of bioemulsifier was produced during the exponential growth phase, and this suggested that the bioemulsifier production was growth-associated. The bioemulsifier showed good emulsification activity on various emulsifying substrates such as hydrocarbons, edible oils, and petroleum fractions.

Cognitive and Behavioral Outcomes of School-aged Children Born Extremely Preterm: a Korean Single-center Study with Long-term Follow-up

Kim Eun Sun,Kim Ee-Kyung,Kim Sae Yun,Song In Gyu,Jung Young Hwa,Shin Seung Han,Kim Han-Suk,Kim Johanna Inhyang,Kim Bung Nyun,Shin Min-Sup 대한의학회 2021 Journal of Korean medical science Vol.36 No.39

Background: School-aged children born very preterm have been suggested to have worse cognitive and behavioral outcomes than children born full-term. Executive function (EF) is a higher level of cognitive function related to academic achievement. The present study aimed to evaluate the cognitive (including EF) and behavioral outcomes of Korean children born extremely preterm (EP) and to analyze any biological or socioeconomic risk factors for poor cognitive outcomes in this population. Methods: A total of 71 infants weighing < 1,000 g at birth or born before 30 weeks of gestation (EP group) who were admitted to the neonatal intensive care unit from 2008 to 2009 were included in this study and compared with 40 term-birth controls. The Korean Wechsler Intelligence Scale for Children-Fourth Edition, Advanced Test of Attention (ATA), Stroop test, Children's Color Trails Test (CCTT), and Wisconsin Card Sorting Test (WCST) were used. Additionally, the Korean Child Behavior Checklist (K-CBCL) and Korean ADHD Rating Scale (K-ARS) were completed. Perinatal and demographic data were collected and analyzed. Results: The mean full-scale intelligence quotient (FSIQ) score in the EP group was significantly lower than that of the term control group (89.1 ± 18.3 vs. 107.1 ± 12.7; P < 0.001). In the EP group, 26 (37%) children had an FSIQ score below 85, compared to only one child (3%) in the control group. Furthermore, the EP group showed significantly worse EF test results (ATA, Stroop test, CCTT, WCST). Except for the higher social immaturity subscore in the EP group, the K-CBCL and K-ARS scores were not different between the two groups. EP children who received laser treatment for retinopathy of prematurity (ROP) had an 8.8-fold increased risk of a low FSIQ score, and a 1-point increase in the discharge weight Z-score decreased the risk of a low FSIQ score by approximately half in this EP cohort. Conclusion: This is the first Korean study to investigate the cognitive and behavioral outcomes of school-aged children born EP. In the study cohort, EP children exhibited significantly lower FSIQ scores and EF than their full-term peers, and 37% of them had cognitive problems. Nonetheless, except for social immaturity, the behavioral problems were not different in EP children. Severe ROP and low discharge weight Z-score were identified as independent risk factors for low FSIQ score after adjusting for birth weight.

The long-term prognosis of nutritional support in preterm infants

( Ee-kyung Kim ) 한국정맥경장영양학회 2016 한국정맥경장영양학회 학술대회집 Vol.2016 No.-

A newborn girl with harlequin ichthyosis genetically confirmed by ABCA12 analysis

Kim, Jihye,Ko, Jung Min,Shin, Seung Han,Kim, Ee-Kyung,Kim, Han-Suk Korean Society of Medical Genetics and Genomics 2019 대한의학유전학회지 Vol.16 No.2

Harlequin ichthyosis (HI, OMIM #242500) is one of the most severe skin diseases among the autosomal recessive congenital ichthyoses, with high morbidity and mortality, particularly in newborns. Clinically, it is characterized by a typical appearance of generalized, thick, yellowish, hyperkeratotic plates with deep erythematous fissures on the skin. Herein, we present the case of a newborn girl with HI that was genetically confirmed by targeted gene panel analysis. The premature baby was encased in an opaque white membrane with erosion covering the skin of the entire body except the lips, with her hands and feet restricted by the membrane. Humidification, emollient, and retinoic acid treatment were started; the thick ichthyosis gradually peeled off and the underlying skin was only covered with thin scales. Targeted gene panel analysis using next-generation sequencing and validation with Sanger sequencing and quantitative polymerase chain reaction analyses confirmed compound heterozygous mutations of the ABCA12 gene (p.N1380S and a partial gene deletion encompassing exon 9). The parents were carriers for each of the identified mutations. Early recognition of the genetic etiology of congenital ichthyosis can, thus, facilitate genetic counseling for patients and their families.

TAZ stimulates liver regeneration through interleukin‐6-induced hepatocyte proliferation and inhibition of cell death after liver injury

Kim, A Rum,I1 Park, Jung,Oh, Ho Taek,Kim, Kyung Min,Hwang, Jun‐,Ha,Jeong, Mi Gyeong,Kim, Ee‐,Hyun,Hwang, Eun Sook,Hong, Jeong‐,Ho Federation of American Society for Experimental Bi 2019 The FASEB Journal Vol.33 No.5

Design of out-coupling structures with metal-dielectric surface relief

Kim, Sun-Kyung,Ee, Ho-Seok,Song, Kyung-Deok,Park, Hong-Gyu The Optical Society 2012 Optics express Vol.20 No.15