Surveillance of hard ticks (Acarina: Ixodidae) as Severe Fever with Thrombocytopenia Syndrome (SFTS) virus vectors in the Republic of Korea, 2022

Sehoon Cho,SoEun Shin,Hyunwoo Kim,Byung-Eon Noh,Tae-Kyu Kim,Wook-Gyo Lee,Hee Il Lee 한국응용곤충학회 2023 한국응용곤충학회 학술대회논문집 Vol.2023 No.04

특허회피설계에 있어서 TRIZ를 사용한 클레임의 기능식 분해분석의 활용에 관한 연구

조세훈(Sehoon Cho) 대한산업공학회 2021 대한산업공학회지 Vol.47 No.4

The effect of oral antibiotics on the skin microbiota in rosacea

( Sehoon Lee ),( Eun Sun Hong ),( Eun Jung Byun ),( Yu Ri Woo ),( Jeong Deuk Lee ),( Sang Hyun Cho ),( Hei Sung Kim ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: Given the widespread use of oral antibiotics for treatment of rosacea, it is important to understand the association of such antibiotic use with changes in the bacterial community of the skin. Objectives: We aimed to see the effect of oral antibiotics on the skin microbiota in rosacea patients Methods: The skin microbiota before and after 6 weeks of oral doxycycline (100 mg, twice a day) were compared. Skin areas on the cheek were sampled for 16s ribosomal RNA gene sequencing. Results: A total of 12 patients enrolled (11 females and 1 male, age: 20-79 years). The percentage of Cutibacterium acnes and Staphylococcus epidermidis were 13% and 28% before treatment. The percentage decreased to 7% (C. acnes) and 22% (S. epidermidis) after 6 weeks of oral doxycycline. Conclusion: Oral antibiotics had some effect on the skin microbial composition in rosacea patients which needs to be further examined.

Theranostic iRGD peptide containing cisplatin prodrug: Dual-cargo tumor penetration for improved imaging and therapy

Cho, Hong-Jun,Park, Sung-Jun,Lee, Yoon-Sik,Kim, Sehoon Elsevier 2019 Journal of controlled release Vol.300 No.-

<P><B>Abstract</B></P> <P>In theranostics, peptide-based platforms have widely been exploited owing to their unique biological functions and chemical versatilities. As a tumor-homing ligand, internalizing RGD peptide (iRGD), composed of a tumor-targeting sequence (RGD) and a cell-penetrating C-end Rule (CendR) motif, is known to facilitate the tumor-specific delivery of payloads that are covalently conjugated on its <I>N</I>-terminal fragment or co-administered without any covalent linkages. However, theranostic uses of the iRGD-based platform remain in its infancy with its full potential unexplored; for instance, detailed mechanism of iRGD fragmentation during internalization, strategies for the tumor-specific release of payloads from iRGD and the role of the <I>C</I>-terminal iRGD fragment in delivery have yet to be revealed. In this study, we designed a dual-channel fluorescent cyclic iRGD (TAMRA-iRGDC-Cy5.5) to track each of the <I>N</I>- and <I>C</I>-terminal fragments separately during the tumor internalization process. It turned out that both fragments undergo translocation into cancer cells together and are localized within endosomal-lysosomal compartments. The resulting co-internalization of both iRGD fragments allowed us to develop a new theranostic peptide platform (Cy5.5-iRGDC-Pt(IV)) by conjugating a fluorescent dye and a cisplatin prodrug on each terminus of cyclic iRGD for simultaneous cancer-targeted imaging and therapy. Compared to a control peptide having a non-iRGD sequence, the Cy5.5-iRGDC-Pt(IV) did not only provide a better tumor imaging contrast but also induced tumor-specific apoptosis leading to efficacious tumor suppression. Besides the outstanding cancer imaging and therapeutic performance, the Cy5.5-iRGDC-Pt(IV) revealed negligible systemic toxicity, holding potential to be applied for theranostic uses.</P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

The effect of oral antibiotics on the skin microbiota characteristics in acne

( Sehoon Lee ),( Eun Sun Hong ),( Eun Jung Byun ),( Yu Ri Woo ),( Jeong Deuk Lee ),( Sang Hyun Cho ),( Hei Sung Kim ) 대한피부과학회 2019 대한피부과학회 학술발표대회집 Vol.71 No.2

Background: With the extensive use of oral antibiotics for treatment of moderate to severe acne, it is important to understand the association of such antibiotic use with changes not only in Cutibacterium acnes (C. acnes) but also in the complete bacterial community of the skin. Objectives: We sought to investigate the changes of skin microbiota in acne patients in association with oral antibiotics. Methods: The skin microbiota of acne patients, before and after taking 6 weeks of oral antibiotics (oral doxycycline, 100mg, twice daily) were compared. Skin areas on the cheek were sampled for 16S ribosomal RNA gene sequencing. Results: Twenty patients were included in this study (11 female, 8 males, ages between 11-44 years). Across all patients, antibiotic treatment was associated with reduction in the levels of C. acnes, Snodgrassella alvi, Corynebacterium matruchotii etc., and an increase in Neisseria oralis, Enterococcus hirae et al. There also was a significant change in microbial diversity represented by Shannon and Inverse Simpson index. Conclusion: In this study, oral antibiotic treatment of acne was associated with changes in the composition and diversity of the skin microbiota.

Genetic variations in HMGCR and PCSK9 and kidney function: a Mendelian randomization study

( Sehoon Park ),( Seong Geun Kim ),( Soojin Lee ),( Yaerim Kim ),( Semin Cho ),( Kwangsoo Kim ),( Yong Chul Kim ),( Seung Seok Han ),( Hajeong Lee ),( Jung Pyo Lee ),( Kwon Wook Joo ),( Chun Soo Lim ) 대한신장학회 2023 Kidney Research and Clinical Practice Vol.42 No.4

Background: The genetically predicted lipid-lowering effect of HMGCR or PCSK9 variant can be used to assess drug proxy effects on kidney function. Methods: Mendelian randomization (MR) analysis-identified HMGCR and PCSK9 genetic variants were used to predict the low-density lipoprotein (LDL) cholesterol-lowering effects of medications targeting related molecules. Primary summary-level outcome data for log-estimated glomerular filtration rate (eGFR; creatinine) were provided by the CKDGen Consortium (n = 1,004,040 European) from a meta-analysis of CKDGen and UK Biobank data. We also conducted a separate investigation of summary-level data from CKDGen (n = 567,460, log-eGFR [creatinine]) and UK Biobank (n = 436,581, log-eGFR [cystatin C]) samples. Summary-level MRs using an inverse variance weighted method and pleiotropy-robust methods were performed. Results: Summary-level MR analysis indicated that the LDL-lowering effect predicted genetically by HMGCR variants (50-mg/dL decrease) was significantly associated with a decrease in eGFR (-1.67%; 95% confidence interval [CI], -2.20% to - 1.13%). Similar significance was found in results from the pleiotropy-robust MR methods when the CKDGen and UK Biobank data were analyzed separately. However, the LDL-lowering effect predicted genetically by PCSK9 variants was significantly associated with an increase in eGFR (+1.17%; 95% CI, 0.10%-2.25%). The results were similarly supported by the weighted median method and in each CKDGen and UK Biobank dataset, but the significance obtained by MR-Egger regression was attenuated. Conclusion: Genetically predicted HMG-CoA reductase inhibition was associated with low eGFR, while genetically predicted PCSK9 inhibition was associated with high eGFR. Clinicians should consider that the direct effect of different types of lipid-lowering medication on kidney function can vary.

Tumor microenvironment-responsive fluorogenic nanoprobe for ratiometric dual-channel imaging of lymph node metastasis

Cho, Hong-Jun,Lee, Seokyung,Park, Sung-Jun,Lee, Yong-Deok,Jeong, Keunsoo,Park, Jae Hyung,Lee, Yoon-Sik,Kim, Bokyung,Jeong, Han-Sin,Kim, Sehoon Elsevier 2019 Colloids and Surfaces B Vol.179 No.-

<P><B>Abstract</B></P> <P>Fluorogenic nanoprobes capable of providing microenvironmental information have extensively been developed to improve the diagnostic accuracy for early or metastatic cancer detection. In cancer-associated microenvironment, matrix metalloproteinase-2,9 (MMP-2,9) has drawn attention as a representative enzymatic marker for diagnosis, prognosis, and prediction of various cancers, which is overexpressed in the primary site as well as metastatic regions. Here, we devised dual-emissive fluorogenic nanoprobe (DFNP) emitting both MMP-2,9-sensitive and insensitive fluorescence signals, for accurate monitoring of the MMP-2,9 activity in metastatic regions. DFNP was nanoscopically constructed by amphiphilic self-assembly between a constantly fluorescent polymer surfactant labeled with Cy7 (F127-Cy7) and an initially nonfluorescent hydrophobic peptide (Cy5.5-MMP-Q) that is fluorogenic in response to MMP-2,9. Ratiometric readout (Cy5.5/Cy7) by dual-channel imaging could normalize the enzyme-responsive sensing signal relative to the constantly emissive internal reference that reflects the probe amount, allowing for semi-quantitative analysis on the MMP-2,9-related tissue microenvironment. In addition to the dual-channel emission, the nanoconstructed colloidal structure of DFNP enabled efficient accumulation to lymph node <I>in vivo</I>. Because of these two colloidal characteristics, when injected intradermally to a mouse model of lymph node metastasis, DFNP could produce reliable ratiometric signals to provide information on the MMP-2,9 activity in the lymph nodes depending on metastatic progression, which corresponded well to the temporal histologic analysis. Furthermore, ratiometric lymph node imaging with DFNP after photodynamic therapy allowed for monitoring a therapeutic response to the given cancer treatment, demonstrating diagnostic and prognostic potential of the nanoconstructed colloidal sensor of tumor microenvironment in cancer treatment.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A dual-emissive fluorogenic nanoprobe was devised for <I>in vivo</I> MMP-2,9 imaging. </LI> <LI> The nanoprobe was efficiently accumulated to lymph nodes through local injection. </LI> <LI> Lymph node metastasis could be monitored by dual-channel imaging of the MMP-2,9 levels. </LI> <LI> Ratiometric readout provided quantitative information on the MMP-2,9 activity <I>in vivo</I>. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

A High-Precision Motion Control Based on a Periodic Adaptive Disturbance Observer in a PMLSM

Kwanghyun Cho,Jonghwa Kim,Seibum Ben Choi,Sehoon Oh IEEE 2015 IEEE/ASME Transactions on Mechatronics Vol.20 No.5

<P>This paper presents a novel disturbance compensation scheme to attenuate periodic disturbances on repetitive motion using permanent magnet linear synchronous motors (PMLSMs), and this scheme is called the periodical adaptive disturbance observer. The scheme is based on assumptions that all measured states and disturbances are periodic and repetitive when the tasks executed by PMLSM motion systems have periodic and repetitive characteristics. In the proposed control scheme, a lumped disturbance is estimated by the classical linear disturbance observer (DOB) for the initial time period and stored in memory storages. It consists of parametric errors multiplied by states, friction force, and force ripple, and then, it is updated for each time period by the periodic adaptation law. This scheme requires no mathematical models of disturbances and adaptation laws of model parameters such as the mass of the mover and viscous friction coefficient. Also, it is possible to compensate for disturbances above as well as below the bandwidth of the Q-filter (LPF) of DOB. The effectiveness of the proposed control scheme is verified by various experiments that take into account varying frequency components of disturbances along the operating speed of a mover of PMLSM such as force ripple and friction force.</P>

VMware 기반의 가상화 OS 에서의 Guest 와 Host OS간의 통신 취약점 연구

박세훈(Sehoon Park),조인순(Insoon Cho),엄현상(Hyeonsang Eom),염헌영(Heonyoung Yeom) 한국정보과학회 2011 한국정보과학회 학술발표논문집 Vol.38 No.2C

분산시스템 컴퓨팅을 위한 가상화기반의 프레임워크가 널리 응용됨에 따라 가상화 환경에서 호스트 OS와 Guest OS가 서로 유기적으로 연결되어 서로 통신을 하게 되는데 그 보안의 취약점에 대해서는 간과하는 면이 많다. 하나의 Host OS 위에서 다수의 Guest OS 를 사용하는 가상화 환경에서 특정 VM(Guest OS) 가 공격받게 되면 Host 시스템뿐만 아니라 다른 VMs 에도 치명적인 악영향을 끼치게 된다. 현재 가상 머신 분야에서 가장 많이 사용되고 있는 VMware사의 Host 와 Guest OS 간의 1) 통신 메커니즘을 분석하고 2) 통신 취약점을 이용한 Host 의 공격 사례 조사 및 분석을 통해서 3) 향후 발생될 가능성 있는 새로운 취약점에 대한 연구를 하는 것이 필요하다.

신형식 콘크리트 헤디드 FRP 보강근 개발

조정래(Cho, Jeong-Rae),박영환(Park, Young-Hwan),박성용(Park, Sung Yong),김성태(Kim, Sung Tae),강세훈(Kang, Sehoon) 대한토목학회 2017 대한토목학회 학술대회 Vol.2017 No.10