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중합효소연쇄반응을 이용한 급성 C 형 간염 환자 혈청에서의 HCV RNA 의 검출
김상우(Sang Woo Kim),백승운(Seung Woon Park),김해엽(Hae Yob Kim),김은주(Eun Joo Kim),문인걸(In Gol Moon),한인권(In Kwon Han) 대한내과학회 1992 대한내과학회지 Vol.43 No.2
N/A Background: Currently used anti-HCV (anti-C100-3) method to detect hepatitis C virus (HCV) shows relatively good sensitivity in the diagnosis of chronic non-A, non-R hepatitis infection, but poor results in acute hepatitis C infectin. Methods: We detected hepatitis C viral RNA (HCV- RNA) in a patient with transfuion-associated acute hepatitis by nested polymerase chain reaction (PCR) method. Results; By nested PCR method, HCV-RNA was detected at 11th week after transfusion, but by anti- C100-3 method, detected at 23th week. Conclusion: We report that the detection of HCV- RNA by nested PCR is more useful than anti-C100-3 in the early diagnosis of acute hepatitis C infection.
( Seoung Yob Han ),( Jong Wook Kim ),( Sang Yun Jeon ),( Heung Joong Kim ),( Byung Ock Kim ),( Joo Cheol Park ),( Chang Hoon Song ),( Hyun Seon Jang ) 한국조직공학·재생의학회 2009 조직공학과 재생의학 Vol.6 No.1
Tissue engineering is attracting attention because it is new approaches for the repair and replacement of tissues and organs that have lost function due to variable reasons. Clinical application have already begun. One of the fundamental principles that underlies tissue engineering strategies is that a newly formed tissue must acquire sufficient vascularization. In recent studies, differentiation of the endothelial cells from variable cell sources has been reported, and vascular endothelial growth factor(VEGF) is very important factor in differentiation. This study investigated whether differentiation of BMSC, cultured with VEGF in vitro, into endothelial cell is possible. BMSC was obtained from Dog BMSC was loaded onto an equal volume of 75ul VEGF and incubated at 37oC in 5% CO2 and 95% humidity. Media was changed every 2days. Total RNA was extracted by use of Trizol reagent. The gene expression of cultured cells was investigated by RT-PCR using the primers - nestin, VE-Cadherin, vWF and GAPDH as internal control. And the PCR products were electrophoresed on a 1.2% agarose gel. Digital images of electrophoresd gel were taken and quantified by densitometer(Bio-rad(R)). As time goes by, experiment group show spindle shape more clearly than control group and cell number increase. Nestin marker is expressed in all groups, beside expression of VE-Cadherin and vWF are higher in experiment group than control group. Experiment group treated with VEGF show high expression of the EC-specific marker: VE-Cadherin and vWF. The fact that 5 days culture group reveals the highest level of gene expression means that this group reachs the sufficient differentiation into EC and that it has potential to reduce the time of vascular tissue regeneration.
이동엽(Dong Yob Lee),강운모(Woon Mo Kang),김아란(Ah Ran Kim),김성대(Sung Dae Kim),라선욱(Seon-Uk Na),고한서(Han Seo Ko) 대한기계학회 2011 대한기계학회 춘추학술대회 Vol.2011 No.10
A role of an oil separator for separation of lil from refrigerant/oil mixture is very important in an air conditioning system because oil can be returned, oil can remain at an optimum level in the compressor, and oil sludge can be reduced by the separator to increase an efficiency of an evaporator. In this study, the flow pattern including the flow velocity has been investigated by a Particle Image Velocity(PIV) technique and a design guideline has been proposed to improve the efficiency of the oil separation. A visualization window has been installed to capture the images of a cyclone flow and to measure the oil separation rate inside the oil separator using the PIV experiment. Velocity profiles have been analyzed numerically by various parameters after selecting an optimum turbulence model. Also, the oil particle ejection has been predicted by performing the oil surface experiment.
과도하게 늘어난 십이지장 윤상주름의 클립 고정법을 이용한 ERCP
김영준,김진홍,조성원,고준호,유병무,한동석,배원엽 대한소화기내시경학회 2000 Clinical Endoscopy Vol.20 No.6
During an endoscopic retrograde cholangiopancreatography (ERCP). cannulation is more difficult in patients with anatomical variations, papillary stenosis, an impacted stone in the papilla, papillary cancer, peripapillary diverticulum, postoperative states such as those after Billroth II or Braun operations, or redundant Kerckring's folds of the duodenum covering the duodenal papilla. For a patient suffering from a redundant Kerckrings folds of the duodenum and a common bile duct stone, a new technique applying clips to expose the duodenal papilla properly during ERCP was performed. After the application of the clips, the duodenal papilla was well exposed in a favorable position for proper cannulation. In this setting, an ERCP was easily performed with the standard cannula. After an endoscopic sphincterotomy (EST) with the pull-type papillotome was conducted, the common bile duct stone was successfully removed with a Dormia basket.
휠체어 농구선수들의 호흡순환기능과 혈관탄성에 관한 연구
김영준(Young Jun Kim),한경희(Gyung Hee Han),김주홍(Ju Hong Kim),이은희(Eun Hee Lee),문성진(Sung Jin Moon),최진관(Jin Goan Choi),장성동(Song Dong Chang),홍명엽(Myeng Yob Hong) 한국사회체육학회 2007 한국사회체육학회지 Vol.0 No.31
This study was purpose to investigate effect of after maximal exercise on cardiorepiratory function and vascular compliance with wheelchair basketball athletes and normal athletes and sedentary. The subjects consisted of spinal cord injured(N=10), basketball athletes(N=10), controls(N=10) that used arm ergometer 30W(watt) loading after warm-up from 0W to all out per 15W. The groups analyzed systolic and diastolic blood pressure, measured on K4b2 through Monark cycle ergometer, vascular compliance through pulse wave velocity. The results were as follows : 1. Cardiorespiratory increased significantly in basketball athletes than spinal cord injured and controls. 2. Vascular compliance was increased in basketball athletes but not significantly. The results suggest that wheelchair athletes was enhanced on cardiorespiratory through basketball. Thus, wheelchair basketball was thought on effected exercise that increased blood volume, pressure, and affirmative effect in sympathetic system.
Molecular Genetic Typing of Legionella pneumophila Strains Isolated in Jejudo, Korea
Kim, Byoung-Jun,Park, Chan-Geun,Kim, Hee-Youn,Han, Chang-Su,Kim, Sung-Yob,Kim, Su-Young,Lee, Jae-Chun,Hong, Seong-Chul,Lee, Keun-Hwa 대한미생물학회 2010 Journal of Bacteriology and Virology Vol.40 No.3
Twenty two strains of Legionella species isolated from Jejudo, Korea were identified by comparing the rpoB (300 bp), dotA (360 bp), and mip (396 bp) gene sequence analysis. Furthermore, their genotypes were determined by sequence analysis of rpoB/dotA subgroup typing, pulse-field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) patterns. Of the 22 isolates, 21 strains were identified as L. pneumophila and 1 strain was close to L. erythra (>95% similarity of rpoB and mip). Most of the L. pneumophila strains (90%) belonged to P-I of rpoB/dotA subgroup typing, one strain of each P-III and P-IV. L. pneumophila isolates were further grouped into 4 and 6 different PFGE (P1 to P4) and RAPD (R1 to R6) patterns, respectively. On the basis of these genotypes, which may be useful for future epidemiological studies, existence of diverse L. pneumophilla population in Jejudo, Korea were observed.
김선아,한상엽,김태형,최영락,윤희성,이경헌,Sun A Kim,Sang Yob Han,Tae Hyung Kim,Yeong Rak Choi,Hee Sung Yoon,Kyung Hun Lee 대한안과학회 2006 대한안과학회지 Vol.47 No.9
Purpose: To report a case of isolated posterior capsule rupture (PCR) with cataract after blunt ocular trauma in a child. Methods: A 6-year-old female visited our institution complaining of blurred vision (best corrected visual acuity: counting fingers at 30 cm) in the left eye three months after blunt ocular trauma. Slitlamp examination showed the cataract and PCR with intact anterior capsule. Removal of the cataract was approached via a scleral tunnel incision. Anterior vitrectomy was performed and a foldable intraocular lens (IOL) was inserted in the sulcus. Results: Visual acuity was improved to 0.7 two days after the surgery and further improved to 1.0 at two months. Conclusions: An isolated PCR with cataract is a rare complication of blunt ocular trauma. We present a case of PCR after blunt ocular injury as well as a surgical procedure to safely remove the cataract via an anterior approach and implantation of the IOL in the sulcus.