Sodium butyrate has context-dependent actions on dipeptidyl peptidase-4 and other metabolic parameters

Lee, Eun-Sol,Lee, Dong-Sung,Pandeya, Prakash Raj,Kim, Youn-Chul,Kang, Dae-Gil,Lee, Ho-Sub,Oh, Byung-Chul,Lee, Dae Ho The Korean Society of Pharmacology 2017 The Korean Journal of Physiology & Pharmacology Vol.21 No.5

Sodium butyrate (SB) has various metabolic actions. However, its effect on dipeptidyl peptidase 4 (DPP-4) needs to be studied further. We aimed to evaluate the metabolic actions of SB, considering its physiologically relevant concentration. We evaluated the effect of SB on regulation of DPP-4 and its other metabolic actions, both in vitro (HepG2 cells and mouse mesangial cells) and in vivo (high fat diet [HFD]-induced obese mice). Ten-week HFD-induced obese C57BL/6J mice were subjected to SB treatment by adding SB to HFD which was maintained for an additional 16 weeks. In HepG2 cells, SB suppressed DPP-4 activity and expression at sub-molar concentrations, whereas it increased DPP-4 activity at a concentration of $1,000{\mu}M$. In HFD-induced obese mice, SB decreased blood glucose, serum levels of insulin and $IL-1{\beta}$, and DPP-4 activity, and suppressed the increase in body weight. On the contrary, various tissues including liver, kidney, and peripheral blood cells showed variable responses of DPP-4 to SB. Especially in the kidney, although DPP-4 activity was decreased by SB in HFD-induced obese mice, it caused an increase in mRNA expression of $TNF-{\alpha}$, IL-6, and $IL-1{\beta}$. The pro-inflammatory actions of SB in the kidney of HFD-induced obese mice were recapitulated by cultured mesangial cell experiments, in which SB stimulated the secretion of several cytokines from cells. Our results showed that SB has differential actions according to its treatment dose and the type of cells and tissues. Thus, further studies are required to evaluate its therapeutic relevance in metabolic diseases including diabetes and obesity.

체육수업에 대한 교과 흥미도가 학생들의 기본적 심리욕구에 미치는 영향

이종철(Lee, Jong-Chul),이상섭(Lee, Sang-Sub) 한국체육과학회 2011 한국체육과학회지 Vol.20 No.6

The purpose of this study is to identify effects of subject interest in physical education on basic psychological desires of students. For doing this, the authors established the students in middle and high schools in Gyeonggi Province as the population, selecting 340 students by using convenience sampling. The collected data was conducted of multiple regression analysis by using the PASW Ver 18.0 statistic program, and the results are as follows. First, the subject interest in physical education had statistically significant effects on the basic psychological desires of the students(competence), but among the sub-factors, teacher preference factors did not have significant effects on the basic psychological desires(competence). Second, all the sub-factors of the subject interest in physical education(teacher preference, subject content, values & efforts, and subject competence) had statistically significant effects on the basic psychological desires of the students(relation). Third, the subject interest in physical education had statistically significant effects on the basic psychological desires of the students(autonomy), but among the sub-factors, teacher preference factors did not have significant effects on the basic psychological desires(autonomy).

체육수업의 재미요인이 학생들의 심리적 유인성향에 미치는 영향

이종철(Lee Jong-Chul),조홍식(Cho Hong-Sik),박윤혁(Park Yoon-Hyuk),이상섭(Lee Sang-Sub) 한국체육과학회 2006 한국체육과학회지 Vol.15 No.3

The purpose of this study was to explore ways to at identifying what factors made students felt enjoyment during physical education class and how much those factors influence on psychological of inducement inclination. To this end, Cluster Random Sampling was used to set up the population with boys and girls middle school students in Kyunggi-do and Inchon-si area. The survey tool was questionnaire and Collected data hereof were analyzed by one way ANOVA analysis and Multiple Regression Analysis with SPSS WIN Ver 11.5. The aforementioned method and procedure led this research to the conclusion as follows. Firstly, out of sub-factors of physical education class enyoyment by middle schoolers' individual characteristics(gender, result), boy students showed more than girl students, in which there were statistically significant difference. Meanwhile, statistically significant difference was not found in free time, explanation and modeling. Secondly, out of sub-factors of psychological of inducement inclination by middle schoolers' individual characteristics(gender, result), boy students' response was higher in senses of confidence, enthusiasm, accomplishment and indulgence than girl students' in which all considers appeared. Thirdly, the effect enjoymant factors of physical education class had statistically significant on sub-factors of psychological of inducement inclination senses of confidence, enthusiasm, accomplishment and indulgence, free time that is a sub-factor of enjoymant which did not had statistically significant influenced on sense of accomplishment.

체육수업에 대한 교과 흥미도가 학생들의 학업열의에 미치는 영향

이상섭(Lee, Sang-Sub),조홍식(Cho, Hong-Sik) 한국체육과학회 2015 한국체육과학회지 Vol.24 No.1

The purpose of this study was to investigate the effects of subject interest in physical education classes on academic engagement among students. For that purpose, the investigator found the population in middle and high school students in Gyeonggi Province and sampled 420 students through convenience sampling. Collected data were put to multiple regression analysis with the PASW Version 18 .0 statistical program. The findings were as follows: First, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement(efficacy). Second, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement(commitment) . Third, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement (vitality). Finally, all the sub-factors of subject interest in physical education classes had statistically significant effects on academic engagement (absorption)

2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242) induces apoptosis via modulating E7 expression and inhibition of PI3K/Akt pathway in SiHa human cervical cancer cells.

Kim, Man Sub,Kim, Jung Hee,Bak, Yesol,Park, Yun Sun,Lee, Dong Hun,Kang, Jeong Woo,Shim, Jung-Hyun,Jeong, Heon Sang,Hong, Jin Tae,Yoon, Do Young Lawrence Erlbaum Associates, Publishers [etc.] 2012 Nutrition and cancer Vol.64 No.8

<P>The Maillard reaction is a chemical reaction occurring between an amino acid and a reducing sugar, usually requiring thermal processing. Maillard reaction products (MRPs) have antioxidant, antimutagenic, and antibacterial effects, and although 2,4-bis (p-hydroxyphenyl)-2-butenal (HPB242), a fructose-tyrosine MRP, appears to inhibit proliferation of cancer cells, its mechanism of action has not been studied in detail. We found that HPB242 treatment modulated expression of cyclins and tumor suppressor genes in SiHa human cervical cancer cell lines: cyclins and phospho-pRB were downregulated, whereas the expression of CDK inhibitors and p53 was enhanced. HPB242 induced apoptosis dose-dependently by suppressing E7 expression and leading to sub-G1 cell-cycle arrest in SiHa cell lines; treatment also led to the proteolytic cleavage of caspase-3, -9, and poly (ADP-ribose) polymerase. Moreover, HPB242 upregulated Fas expression, altered expressions of pro- and antiapoptotic factors, and also inhibited nuclear translocation of nuclear factor κB and phosphorylation of IκB. HPB242 treatment decreased phosphatidyl inositol-3 kinase and p-Akt expression levels, demonstrating that this survival pathway may also be inhibited by HPB242. Cumulatively, HPB242 promotes apoptosis by influencing E7 expression, inducing cell-cycle arrest at sub-G1 phase, and promoting both intrinsic (mitochondrial) and extrinsic (Fas-dependent) apoptosis in SiHa human cervical cancer cells.</P>

Anti-Cancer Effect of Ginsenoside F₂ against Glioblastoma Multiforme in Xenograft Model in SD Rats

Shin, Ji-Yon,Lee, Jung-Min,Shin, Heon-Sub,Park, Sang-Yong,Yang, Jung-Eun,KimCho, So-Mi,Yi, Tae-Hoo The Korean Society of Ginseng 2012 Journal of Ginseng Research Vol.36 No.1

The glioblastoma multiforme (GBM) is the most common malignant brain tumor in adults. Despite combination treatments of radiation and chemotherapy, the survival periods are very short. Therefore, this study was conducted to assess the potential of ginsenoside $F_2$ (F2) to treat GBM. In in vitro experiments with glioblastoma cells U373MG, F2 showed the cytotoxic effect with $IC_{50}$ of 50 ${\mu}g/mL$ through apoptosis, confirmed by DNA condensation and fragmentation. The cell population of cell cycle sub-G1 as indicative of apoptosis was also increased. In xenograft model in SD rats, F2 at dosage of 35 mg/kg weight was intravenously injected every two days. This reduced the tumor growth in magnetic resonance imaging images. The immunohistochemistry revealed that the anticancer activity might be mediated through inhibition of proliferation judged by Ki67 and apoptosis induced by activation of caspase-3 and -8. And the lowered expression of CD31 showed the reduction in blood vessel densities. The expression of matrix metalloproteinase-9 for invasion of cancer was also inhibited. The cell populations with cancer stem cell markers of CD133 and nestin were reduced. The results of this study suggested that F2 could be a new potential chemotherapeutic drug for GBM treatment by inhibiting the growth and invasion of cancer.

High-throughput screening of protein variations in soybean seeds.

Vijayanand Velusamy,Kyung Jun Lee,Bo-Keun Ha,Jin Baek Kim,Sang Hoon Kim,Si-Yong Kang,Dong Sub Kim 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

The protein in soybean seeds accounts for approximately 40% of the dry seed weight. Two major storage proteins, 7S and 11S, constitute 70-80% of the total storage proteins in the seeds. In this study, the variation of total soluble protein extracts from 1152 soybean landraces that have been collected from South Korea were studied using high-throughput screening method with HT Protein Express Labchip (Caliper Life Sciences, Inc.). Seven distinct protein band patterns - four protein sub-units of 11S and three sub-units of 7S, were taken into account and their presence or absence were analyzed. Among the 1152 landraces, 525 genotypes were identified as lacking lipoxygenase, 255 lacking α1 subunit, 680 lacking α subunit, 169 lacking β subunit, 140 lacking acidic, 114 lacking Kunitz Trypsin Inhibitor (KTi) and 199 lacking basic protein patterns. The high-throughput protein analysis is helpful in screening a large number of populations with less time and minimum labor. The selected genotypes with low amounts or lacking of anti-nutritional factors such as trypsin inhibitor, lipoxygenase and α subunit would be used for future breeding purpose of quality improvement in soybean protein.

일부 직업인의 건강증진생활양식에 영향을 미치는 요인 연구

이은경,안병상,유택수,김성천,정재열,박용신,장두섭,송용선,이기남,Lee Eun-Kyoung,An Byung-Sang,Yu Taek-Su,Kim Seoung-Cheon,Jeung Jea-Yeal,Park Young-Shin,Jahng Doo-Sub,Song Yung-Sun,Lee Ki-Nam 대한예방한의학회 2000 대한예방한의학회지 Vol.4 No.2

The current industrial health service is shifting to health improvement business with 1st primary prevention-focused service from secondary and tertiary prevention-focused business, and Oriental medicine can provide such primary prevention-focused service due to the characteristics of its science. In particular, the advanced concept of health improvement can match the science of health care of Oriental medicine. Notably, what is most important in health improvement is our lifestyle, This does not underestimate the socio-environmental factors, which have lessened their importance due to modernism. The approach of Oriental medicine weighs more individuals' lifestyle and health care through self-cultivation. This matches the new model of advanced health business. Oriental medicine is less systemized than Western medicine, but it can provide ample contents that enhance health. If we conceive health-improvement program based on the advantages provided by these two medical systems, this will influence workers to the benefit of their health. Also, health Program needs to define factors that determine individual lives, and to provide information and technologies essential to our lives. The Oriental medicine approach puts more stress on a subject's capabilities than it does on the effect his surrounding environment can have. This needs to be supported theoretically by not only defining the relations between an individual's health state and his lifestyle, but also identifying the degree to which an individual in the industrial work place practices health improvement lifestyle . This is the first step toward initiating health-improvement business . In order to do this, this researcher conducted a survey by taking random samplings from workers, and can draw the following conclusions from it. 1 The sampled group is categorized into', by sender, female 6.6%, and male 93.4%, with males dominant; by marriage status , unmarried 43.9% and married 55.6%, with both similar percentage, and, by age, below 30, 48.4%, between 30 and 39, 27.4%, between 40 and 49, 18.2%, and over 50, 6.0%. The group further is categorized into; by education, middle school or under 1.7%, high school 30.5%, and junior college or higher 65.8% with high school and higher dominant: and by income, below 1.7 million won 24.2%, below 2.4 million won 14.8%, and above 2.4 million 6.3% Still, the group by job is categorized into collegians with 23.9%, office worker with 10.3%, and professionals with 65.8% , and this group does not include workers engaged in production that are needed for this research, but mostly office workers . 2. The subjects selected for this survey show their degree of practicing health-improvement lifestyle at an average of 2.63, health management pattern at 2.64, and health-related awareness at 2.62 The sub-divisions of health-improvement lifestyle show social emotion (2.87), food (2.66). favorite food (2.59), and leisure activities (2.52), in this order for higher points. It further shows health awareness (2.47) and safety awareness (2.40), lower points than those in health management pattern . 3. In the area of using leisure time for health-improvement, males, older people, married, and people with higher income earn higher marks. And, in the area of food management, the older and married earn higher marks . In the area of favorite food management, females, lower-income bracket, and lower-educated show higher degree of practice , while in the area of social emotion management, the older. married, and higher-income bracket show higher marks. In addition, in the area of health awareness, the older, married, and people with higher-income show higher degree of practice. 4. To look at correlation by overall and divisional health-improvement practice degree , this researcher has analyzed the data using Person's correlation coefficient. The lifestyle shows significant correlation with its six sub-divisions, and use of leisure time, food, and health awareness al

도파민 수송체의 C-말단이 도파민 수송체 기능에 미치는 영향

이준규,박병섭,김용석,이상훈,이용성 漢陽大學校 精神健康硏究 1998 精神健康硏究 Vol.17 No.-

Sodium Azide와 2-AF에 대한 마(Dioscorea batatas Decene)의 항변이원성

이임선,정세영,심창섭,구성자 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.2

The objective of this study was to screen the antimutagenicity of yam enzymatic browning reaction product(YEBRP), mucopolysaccharide and dietary fiber from yam to the mutagen of sodium azide and 2-aminoflourene(2-AF). Antimutagenicity of YEBRP on the mutagenicity of sodium azide showed no difference compared to control without YEBRP but that of 2-AF was high in all substrate.(P<0.01) On the mutagenicity of sodium azide and 2-AF, antimutagenicity of mucopolysaccharide and dietary fiber were high (p<0.01) in α-cellulose and hemicellulose. Antimutagenicity of α-cellulose on the mutagenicity of 2-AF was high at 5 hours reaction time but that was decreased as the reaction time increased.