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RISS 인기검색어
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- 저자 : Qi Yanping (검색결과 6 건)
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Yanping Yao,Chunling Liu,Haidong Qi,Xi Chang,Chunwu Wang,Guangde Wang 한국물리학회 2011 Current Applied Physics Vol.11 No.3
Temperature dependence of dark conductivity and photoconductivity has been studied in amorphous InSb thin films in the temperature range of 120―300 K. The experimental data suggest that conduction in the high temperature range occurs in the extended states; conduction in the intermediate temperature range is due to thermally assisted tunneling of charge carriers in localized states near the band edge; while conduction in low temperature range takes place through variable range hopping of charge carriers in the localized states near the Fermi level. The temperature dependence of photoconductivity shows that the temperature region is divided into two regions.Moreover the result of intensity dependence of steady state photoconductivity indicates that bimolecular recombination at high temperatures and monomolecular at low temperatures in amorphous InSb thin films.
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Expression and purification of beefy meaty peptide in Pichia pastoris
Yanping Wang,Qi Meng,Wen Gao,Jianhua Hou,Zaheer Ahmed 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.3
We established a gene expression process to produce a flavor peptide in Pichia pastoris. The octapeptide Lys-Gly-Asp-Glu-Glu-Ser-Leu-Ala was isolated from beef digested by papain, known as “beefy meaty peptide” (BMP). It was considered a savory seasoning in previous literatures. To produce BMP by microorganisms, BMP expression structures encoding tandem repeats of the octapeptide were designed and cloned in E. coli DH5α, then integrated into the AOX1 gene of Pichia pastoris GS115. Clones containing different BMP-gene copies, with 4-copy, 8-copy, 12-copy and 16-copy, were obtained and expressed in Pichia pastoris GS115. For the ease of purification, 6×His tag was fused to the C-termini of the peptides. As a result, the fusion peptides were successfully purified by His-tag bind affinity resin. The BMP fusion peptides with expected sizes were secreted from the resulting strains of P. pastoris GS115.
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Removal of chloride ions from acidic solution with antimony oxides
Xuewen Wang,Yanping Du,Haoxiang Yang,Shenghui Tian,Qi Ge,Sheng Huang,Mingyu Wang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.93 No.-
This studyaims to investigate the removal of Cl- from acidic solutionwith antimony oxides. The effects of theadsorbenttype,reaction time,reaction temperature,Sb/Clmole ratio andacidityonadsorptionperformanceand the regeneration of loaded adsorbent were systematically studied. The results shows that Sb2O3 xH2Ohas the highest adsorption rate (97.64%) among Sb2O3 xH2O, Sb2O5 xH2O, Sb2O4 xH2O and Sb2O3 in thesolution containing 1.25 mol/L H2SO4under the condition of Sb/Cl mole ratio 3:1 and stirring for 2 h at roomtemperature, and the concentration of Cl- in the acid solution can be reduced to 280 mg/L. Then the residualSb in the adsorbed solution can be decreased from 48.21 mg/L to 16.09 mg/L by C-SbA which is made bySb2O5 xH2O. The C-SbA which has been used can be reused after calcining at 400 ℃ for 2 h. The loadedadsorbent was completely regenerated by adding it into NaOH or Na2CO3 solution whose pH is equal ormore than 9.5 according to S/L ratio 1:6 g/mL and was stirred for 0.5 h at room temperature. The Cl- in theregenerated solution was crystallized and precipitated in the form of NaCl without evaporation andconcentration according to the common ion effect of Na+, and the purity of NaCl was more than 99%.
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Dawei Gao,Min Zhao,Ximing Qi,Yanping Liu,Nan Li,Zhiwei Liu,Yanhong Bian 대한약학회 2016 Archives of Pharmacal Research Vol.39 No.2
Gynostemma pentaphyllum (GP) is a natural plant resources for diabetes therapy, however, there is little research on the mechanisms of GP. The present study was undertaken to characterize if G. pentaphyllum saponins (GPs) is the principal active compound of GP responsible for anti-diabetes, and to examine the relativity between blood glucose modulate and antioxidation. The GPs-treated streptozotocin diabetic rats had a more effective hypoglycemic status than those of diabetic control rats, which also ameliorate dyslipidemia. GPs has increased SOD and GSH-px activities, and the spleen and thymus indexes in diabetic rats. The insulin levels in the GPs-treated groups were significantly higher than diabetic control group. Our finding provides a new insight into the application of GPs for the treatment of oxidative stress related diseases.
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Liu Dan,Xing Ruinan,Zhang Quanyu,Tian Xiaoxiang,Qi Yanping,Song Haixu,Liu Yanxia,Yu Haibo,Zhang Xiaolin,Jing Quanmin,Yan Chenghui,Han Yaling 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Autophagy plays an important role in the development of diabetic cardiomyopathy. Cellular repressor of E1A-stimulated genes 1 (CREG1) is an important myocardial protective factor. The aim of this study was to investigate the effects and mechanisms of CREG1 in diabetic cardiomyopathy. Male C57BL/6 J mice, Creg1 transgenic mice and cardiac-specific knockout mice were used to establish a type 2 diabetes model. Small animal ultrasound, Masson’s staining and western blotting were used to evaluate cardiac function, myocardial fibrosis and autophagy. Neonatal mouse cardiomyocytes (NMCMs) were stimulated with palmitate, and the effects of CREG1 on NMCMs autophagy were examined. CREG1 deficiency exacerbated cardiac dysfunction, cardiac hypertrophy and fibrosis in mice with diabetic cardiomyopathy, which was accompanied by exacerbated autophagy dysfunction. CREG1 overexpression improved cardiac function and ameliorated cardiac hypertrophy and fibrosis in diabetic cardiomyopathy by improving autophagy. CREG1 protein expression was decreased in palmitate-induced NMCMs. CREG1 knockdown exacerbated cardiomyocyte hypertrophy and inhibited autophagy. CREG1 overexpression inhibited cardiomyocyte hypertrophy and improved autophagy. LAMP2 overexpression reversed the effect of CREG1 knockdown on palmitate-induced inhibition of cardiomyocyte autophagy. CREG1 inhibited LAMP2 protein degradation by inhibiting the protein expression of F-box protein 27 (FBXO27). Our findings indicate new roles of CREG1 in the development of diabetic cardiomyopathy.
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He, Meian,Xu, Min,Zhang, Ben,Liang, Jun,Chen, Peng,Lee, Jong-Young,Johnson, Todd A.,Li, Huaixing,Yang, Xiaobo,Dai, Juncheng,Liang, Liming,Gui, Lixuan,Qi, Qibin,Huang, Jinyan,Li, Yanping,Adair, Linda S IRL Press 2015 Human molecular genetics Vol.24 No.6
<P>Human height is associated with risk of multiple diseases and is profoundly determined by an individual's genetic makeup and shows a high degree of ethnic heterogeneity. Large-scale genome-wide association (GWA) analyses of adult height in Europeans have identified nearly 180 genetic loci. A recent study showed high replicability of results from Europeans-based GWA studies in Asians; however, population-specific loci may exist due to distinct linkage disequilibrium patterns. We carried out a GWA meta-analysis in 93 926 individuals from East Asia. We identified 98 loci, including 17 novel and 81 previously reported loci, associated with height at <I>P</I> < 5 × 10<SUP>−8</SUP>, together explaining 8.89% of phenotypic variance. Among the newly identified variants, 10 are commonly distributed (minor allele frequency, MAF > 5%) in Europeans, with comparable frequencies with in Asians, and 7 single-nucleotide polymorphisms are with low frequency (MAF < 5%) in Europeans. In addition, our data suggest that novel biological pathway such as the protein tyrosine phosphatase family is involved in regulation of height. The findings from this study considerably expand our knowledge of the genetic architecture of human height in Asians.</P>
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