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- 저자 : Haoxiang Yang (검색결과 2 건)
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Removal of chloride ions from acidic solution with antimony oxides
Xuewen Wang,Yanping Du,Haoxiang Yang,Shenghui Tian,Qi Ge,Sheng Huang,Mingyu Wang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.93 No.-
This studyaims to investigate the removal of Cl- from acidic solutionwith antimony oxides. The effects of theadsorbenttype,reaction time,reaction temperature,Sb/Clmole ratio andacidityonadsorptionperformanceand the regeneration of loaded adsorbent were systematically studied. The results shows that Sb2O3 xH2Ohas the highest adsorption rate (97.64%) among Sb2O3 xH2O, Sb2O5 xH2O, Sb2O4 xH2O and Sb2O3 in thesolution containing 1.25 mol/L H2SO4under the condition of Sb/Cl mole ratio 3:1 and stirring for 2 h at roomtemperature, and the concentration of Cl- in the acid solution can be reduced to 280 mg/L. Then the residualSb in the adsorbed solution can be decreased from 48.21 mg/L to 16.09 mg/L by C-SbA which is made bySb2O5 xH2O. The C-SbA which has been used can be reused after calcining at 400 ℃ for 2 h. The loadedadsorbent was completely regenerated by adding it into NaOH or Na2CO3 solution whose pH is equal ormore than 9.5 according to S/L ratio 1:6 g/mL and was stirred for 0.5 h at room temperature. The Cl- in theregenerated solution was crystallized and precipitated in the form of NaCl without evaporation andconcentration according to the common ion effect of Na+, and the purity of NaCl was more than 99%.
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Identification of DNA methylation and genetic alteration simultaneously from a single blood biopsy
Chen Xiaomin,Liu Jiahui,Li Jun,Xie Yinpeng,Yu Zichen,Shen Lu,Liu Qingfeng,Wu Wei,Zhao Qiang,Lin Haoxiang,Liu Gaotong,Luo Qiuping,Yang Ling,Huang Yi,Zhao Meiru,Yi Xin,Xia Xuefeng 한국유전학회 2023 Genes & Genomics Vol.45 No.5
Background High-throughput sequencing of blood cell-free DNA (cfDNA) techniques offer an opportunity to characterize and monitor cancer rapidly in a non-invasive and real-time manner. Nonetheless, there lacks a tool within therapeutic arsenal to identify multi-omics alterations simultaneously from a single biopsy. In current times, bisulfite-based sequencing detects 5mC and 5hmC at single-base resolution is the golden standard of DNA methylation, while the degradation of DNA and biased sequencing data are the problems of this method. Objective To identify the consistency analysis of methylation and genetic variation with single library, we presented a platform detecting multi-omics data simultaneously from a single blood biopsy using bisulfite-free method of genomic methylation sequencing (GM-seq) mediated by TET enzyme. Methods We detected methylomic and genetic changes simultaneously from a single blood biopsy in NA12878 and randomly chose ten blood biopsies from colorectal cancer or lung cancer patients to validate the ability of GM-seq. Results Similar cytosine methylation level between whole genome bisulfite sequencing (WGBS) and GM-seq were identified in NA12878. Moreover, longer insert size, CpGs coverage and GC distribution were outperformed than WGBS. In addition, the comparison of the single nucleotide polymorphism (SNP), insertion-deletion (Indel) and copy number variation (CNV) in NA12878 or ctDNA from liver cancer between GM-seq and whole genome sequencing (WGS) show a good consistency, indicating that this method is feasible for detecting genetic variation in blood. Conclusion In conclusion, our work demonstrated a method for identification of the methylated modification and genetic variations simultaneously from a single blood biopsy.
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