The USP21/YY1/SNHG16 axis contributes to tumor proliferation, migration, and invasion of non-small-cell lung cancer

Pei Xu,Haibo Xiao,Qi Yang,Rui Hu,Lianyong Jiang,Rui Bi,Xueyan Jiang,Lei Wang,Ju Mei,Fangbao Ding,Jianbing Huang 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

Deubiquitinases (DUBs) and noncoding RNAs have been the subjects of recent extensive studies regarding their roles in lung cancer, but the mechanisms involved are largely unknown. In our study, we used The Cancer Genome Atlas data set and bioinformatics analyses and identified USP21, a DUB, as a potential contributor to oncogenesis in nonsmall-cell lung cancer (NSCLC). We further demonstrated that USP21 was highly expressed in NSCLCs. We then conducted a series of in vitro and in vivo assays to explore the effect of USP21 on NSCLC progression and the underlying mechanism involved. USP21 promoted NSCLC cell proliferation, migration, and invasion and in vivo tumor growth by stabilizing a well-known oncogene, Yin Yang-1 (YY1), via mediating its deubiquitination. Furthermore, YY1 transcriptionally regulates the expression of SNHG16. Moreover, StarBase bioinformatics analyses predicted that miR4500 targets SNHG16 and USP21. A series of in vitro experiments indicated that SNHG16 increased the expression of USP21 through miR-4500. In summary, the USP21/YY1/SNHG16 axis plays a role in promoting the progression of NSCLC. Therefore, the USP21/YY1/SNHG16/miR-4500 axis may be a potential therapeutic target in NSCLC treatment.

마그네슘 풍부 해양미네랄 용액이 hairless 마우스의 아토피성 피부염에 미치는 영향

김동희,이규재,최주봉,이영미,윤양숙,김정례,장병수,양용석 韓國電子顯微鏡學會 2008 Applied microscopy Vol.38 No.3

Atopic dermatitis (AD) is a chronically relapsing inflammatory skin disease that often has asthma and allergic rhinitis. Magnesium salts, the important component of minerals in Dead Sea water, are known to exhibit beneficial effects in inflammatory disease. Favorable effects of magnesium ions and sea water treated to the skin of patients with contact dermatitis have been reported. But histological and immunological investigations are insufficient. This study was performed to examine the inhibitory effect of magnesium-rich sea mineral water on the development of AD-like skin lesions in hairless mice. AD-like skin lesions are induced by the repeated application of 2,4-dinitrochlorobenzene (DNCB). Local application of magnesium-rich sea mineral water on hairless mice skin applied with DNCB inhibited the development of AD-like skin lesions as exemplified by a significant increase in skin hydration (p<0.01), and a decrease in epidermal water loss (p<0.01). Serum IgE level was also significantly decreased (p<0.01). These results suggest that magnesiumrich sea mineral water inhibits the development of DNCB-induced AD-like skin lesions in hairless mice. These observations indicate that magnesium-rich sea mineral water may be alternative and assistant substances for the management of AD. 아토피성 피부염은 주로 천식과 비염 등을 동반하는, 주위에서 흔히 볼 수 있는 만성 염증성 피부질환으로 유전학적, 환경적, 면역학적 요인이 복잡하게 연관되어 발병한다. 해수에 포함된 마그네슘염은 피부에 작용하여 피부장벽을 보호하는 것으로 알려지고 그에 대한 면역학적인 연구와 조직학적 연구는 아직 부족한 실정이다. 이번 연구에서는 피부염을 인위적으로 일으키는 hapten 형성물질인 DNCB를 hairless mice에 도포하여 아토피 피부염 동물 모델로 만든 후, 마그네슘이 다량 함유된 해양 미네랄수를 처리한 후 피부장벽에 미치는 영향을 관찰하였다. DNCB로 피부염을 유발한 hairless mice에 해양미네랄수를 국소적으로 도포하였을 때 유의한 피부수분함량이 증가와 경피수분손실의 감소를 확인하였다 (p<0.01). 피부측정에서 피부거칠기(skin roughness, p<0.05)와 스케일생성 (skin scaliness, p<0.01)은 실험군에서 유의한 개선효과를 나타내었으며 조직학적 검사에서도 피부손상지수의 유의한 감소 (p<0.01)와 비만세포와 (p<0.01) 호산구의 감소(p<0.05) 소견을 보였고 또한 혈청 IgE의 감소를 관찰할 수 있었다(p<0.01). 이상과 같이 마그네슘이 다량 함유된 해양 미네랄수 도포는 피부장벽의 손상을 줄이고 피부수분손실을 효과적으로 줄임으로 아토피성 피부염 증상 유발을 억제할수 있음을 확인하였다. 현재까지 아토피성 피부염의 관리를 위하여 세라마이드나 식물성 오일의 보습제가 주로 활용되고 있는 상황에서 부가적인 피부장벽의 보호를 위하여 탈염 해양 미네랄수의 활용이 가능할 것으로 판단되며 장기적으로 아토피 피부염치료의 대체, 혹은 보조적 물질로 활용될 수 있을 것으로 기대된다.

Effects of gas signaling molecule SO2 in cardiac functions of hyperthyroid rats

Yang Qi,Yang Ting,Liu Xing,Liu Shengquan,Liu Wei,Nie Liangui,Chu Chun,Yang Jun 대한약리학회 2024 The Korean Journal of Physiology & Pharmacology Vol.28 No.2

Sulfur dioxide (SO2), a novel endogenous gas signaling molecule, is involved in the regulation of cardiac function. Exerting a key role in progression of hyperthyroidism-induced cardiomyopathy (HTC), myocardial fibrosis is mainly caused by myocardial apoptosis, leading to poor treatment outcomes and prognoses. This study aimed to investigate the effect of SO2 on the hyperthyroidism-induced myocardial fibrosis and the underlying regulatory mechanisms. Elisa, Masson staining, Western-Blot, transmission electron microscope, and immunofluorescence were employed to evaluate the myocardial interstitial collagen deposition, endoplasmic reticulum stress (ERS), apoptosis, changes in endogenous SO2, and Hippo pathways from in vitro and in vivo experiments. The study results indicated that the hyperthyroidism-induced myocardial fibrosis was accompanied by decreased cardiac function, and down-regulated ERS, apoptosis, and endogenous SO2-producing enzyme aspartate aminotransferase (AAT)1/2 in cardiac myocytes. In contrast, exogenous SO2 donors improved cardiac function, reduced myocardial interstitial collagen deposition, up-regulated AAT1/2, antagonized ERS and apoptosis, and inhibited excessive activation of Hippo pathway in hyperthyroid rats. In conclusion, the results herein suggested that SO2 inhibited the overactivation of the Hippo pathway, antagonized ERS and apoptosis, and alleviated myocardial fibrosis in hyperthyroid rats. Therefore, this study was expected to identify intervention targets and new strategies for prevention and treatment of HTC.

Biomechanical Evaluation of 2 Endoscopic Spine Surgery Methods for Treating Lumbar Disc Herniation: A Finite Element Study

Yang Zou,Shuo Ji,Hui Wen Yang,Tao Ma,Yue Kun Fang,Zhi Cheng Wang,Miao Miao Liu,Ping Hui Zhou,Zheng Qi Bao,Chang Chun Zhang,Yu Chen Ye 대한척추신경외과학회 2024 Neurospine Vol.21 No.1

Objective: This study aimed to evaluate the effects of 2 endoscopic spine surgeries on the biomechanical properties of normal and osteoporotic spines. Methods: Based on computed tomography images of a healthy adult volunteer, 6 finite element models were created. After validating the normal intact model, a concentrated force of 400 N and a moment of 7.5 Nm were exerted on the upper surface of L3 to simulate 6 physiological activities of the spine. Five types of indices were used to assess the biomechanical properties of the 6 models, range of motion (ROM), maximum displacement value, intervertebral disc stress, maximum stress value, and articular protrusion stress, and by combining them with finite element stress cloud. Results: In normal and osteoporotic spines, there was no meaningful change in ROM or disc stress in the 2 surgical models for the 6 motion states. Model N1 (osteoporotic percutaneous transforaminal endoscopic discectomy model) showed a decrease in maximum displacement value of 20.28% in right lateral bending. Model M2 (unilateral biportal endoscopic model) increased maximum displacement values of 16.88% and 17.82% during left and right lateral bending, respectively. The maximum stress value of L4–5 increased by 11.72% for model M2 during left rotation. In addition, using the same surgical approach, ROM, maximum displacement values, disc stress, and maximum stress values were more significant in the osteoporotic model than in the normal model. Conclusion: In both normal and osteoporotic spines, both surgical approaches were less disruptive to the physiologic structure of the spine. Furthermore, using the same endoscopic spine surgery, normal spine biomechanical properties are superior to osteoporotic spines.

Study on Large Straight Blade Wide Impeller

Qi Chun Song,Dan Hui Jie,Yang Yang Zhe,Dong Kang Tian 대한기계학회 1997 International Conference on Fluid Machinery Vol.1 No.1

Two new species of Matileola (Diptera, Keroplatidae) from China

Yang Qi-Cheng,Liu Xiao-Yan,Yang Ding 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.1

Only three species belong to Matileola in previously described species of Keroplatidae from world. Here two species are added to the fauna of Chinese mainland, Matileola motuoensis sp. nov. and Matileola sejilaensis sp. nov., which are described and illustrated as new to science. A key to the species of Matileola from world is presented. LSID: urn:lsid:zoobank.org:pub:09701DF4-61B5-42E4-BF05-EC9E68718758.

Asynchronous Emergence Mechanisms of Anatatus orientalis (Hymenoptera: Eupelmidae), an Egg Parasitoid of Lantern-fly Lycorma delicatula (Hemiptera: Fulgoridae)

Yang Zhong-qi,Choi Man-Young,Lee Hwi Jong,Byeon Young Woong 한국응용곤충학회 2014 한국응용곤충학회 학술대회논문집 Vol.2014 No.04

Anatatus orientalis is an important natural enemy parasitizing eggs of lanternfly Lycorma delicatula White. In order to effectively utilize this parasitoid, the parasitism rates of lantern-fly and emergence of its parasitoid were surveyed in different areas. Results showed that the emergences of wasp Anatatus orientalis from different areas had significant differences. Those parasitoids emerged from lantern-fly eggs collected from Yangling of Shaanxi province, Qinhuangdao of Hebei province, and Dagang District of Tianjin City came out focus on May only, while wasps from Yantai of Shandong province showed 2 obvious emergence peaks in May and September respectively. Wasp populations with emergence asynchrony in Yantai area were analyzed by different periods. Results revealed that the emergence percentages in spring (May) were significantly lower than that in fall (September), but the sex ratios did not differ significantly between the two seasons. The emergence asynchrony of parasitoid may be induced by the diapause of species, which is a long-term coevolutional outcome of parasitoid with host for maintaining its population. These findings provided some scientific bases for the rational utilization of this parasitoid in practice.

Influence of a Flexible Retaining Wall on Performance of an Adjacent Deep Excavation

Qi-Run Yang,Ming-Guang Li,Jin-Jian Chen 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.2

In urban areas with scarce land resources, many deep excavations are performed adjacent to flexible retaining walls. This study investigated the influence of a flexible retaining wall on the performance of an adjacent deep excavation through numerical modeling. The numerical method incorporating the hardening soil small-strain model and input parameters were primarily validated using a well-documented case. Hypothetical models with different spacings between the deep excavation and existing flexible retaining wall were established to study the deformation behavior of diaphragm walls and characteristics of lateral earth pressure. Parametric studies were carried out to investigated the influence of the excavation width, wall depth, and bending rigidity of retaining walls on the performance of the excavation. Finally, the mechanisms of the flexible retaining wall to influence the mobilization of the arching effect in confined soil were discussed. Results show that the adjacent flexible retaining wall aggravates maximum wall deformation and leads to the inclination of the excavation. The impact is mainly attributed to the redistribution of lateral earth pressure resulted from the mobilization of the arching effect. However, the influence of the flexible retaining wall on the magnitude of the resultant force of lateral earth pressure is insignificant. Both qualitative analysis and numerical results prove that the general shape of the major principal stress trace is a curve convex to stabilized soil when the arching effect is generated.

An experimental study on fracture coalescence characteristics of brittle sandstone specimens combined various flaws

Yang, Sheng-Qi Techno-Press 2015 Geomechanics & engineering Vol.8 No.4

This research aims to analyze the fracture coalescence characteristics of brittle sandstone specimen ($80{\times}160{\times}30mm$ in size) containing various flaws (a single fissure, double squares and combined flaws). Using a rock mechanics servo-controlled testing system, the strength and deformation behaviours of sandstone specimen containing various flaws are experimentally investigated. The results show that the crack initiation stress, uniaxial compressive strength and peak axial strain of specimen containing a single fissure are all higher than those containing double squares, while which are higher than those containing combined flaws. For sandstone specimen containing combined flaws, the uniaxial compressive strength of sandstone increase as fissure angle (${\alpha}$) increases from $30^{\circ}$ to $90^{\circ}$, which indicates that the specimens with steeper fissure angles can support higher axial capacity for ${\alpha}$ greater than $30^{\circ}$. In the entire deformation process of flawed sandstone specimen, crack evolution process is discussed detailed using photographic monitoring technique. For the specimen containing a single fissure, tensile wing cracks are first initiated at the upper and under tips of fissure, and anti-tensile cracks and far-field cracks are also observed in the deformation process; moreover anti-tensile cracks usually accompanies with tensile wing cracks. For the specimen containing double squares, tensile cracks are usually initiated from the top and bottom edge of two squares along the direction of axial stress, and in the process of final unstable failure, more vertical splitting failures are observed in the ligament region. When a single fissure and double squares are formed together into combined flaws, the crack coalescence between the fissure tips and double squares plays a significant role for ultimate failure of the specimen containing combined flaws.

Ultrasonographic Features of Triple-Negative Breast Cancer: a Comparison with Other Breast Cancer Subtypes

Yang, Qi,Liu, Hong-Yan,Liu, Dan,Song, Yan-Qiu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

Background: Triple-negative breast cancer (TNBC) is known to be associated with aggressive biologic features and a poor clinical outcome. Therefore, early detection of TNBC without missed diagnosis is a requirement to improve prognosis. Preoperative ultrasound features of TNBC may potentially assist in early diagnosis as characteristics of disease. Purpose: To retrospectively evaluate the sonographic features of TNBC compared to ER (+) cancers which include HER(-) and HER2 (+), and HER2 (+) cancers which are ER (-). Materials and Methods: From June 2012 through June 2014, sonographic features of 321 surgically confirmed ER (+) cancers (n=214), HER2 (+) cancers (n=66), and TNBC (n=41) were retrospectively reviewed by two ultrasound specialists in consensus. The preoperative ultrasound and clinicopathological features were compared between the three subtypes. In addition, all cases were analyzed using morphologic criteria of the ACR BI-RADS lexicon. Results: Ultrasonographically, TNBC presented as microlobulated nodules without microcalcification (p=0.034). A lower incidence of ductal carcinoma in situ (p<0.001), invasive tumor size that is>2 cm (p=0.011) and BI-RADS category 4 (p<0.001) were significantly associated with TNBC. With regard to morphologic features of 41 TNBC cases, ultrasonographically were most likely to be masses with irregular (70.7%) microlobulated shape (48.8%), be circumscribed (17.1%) or have indistinct margins (17.1%) and parallel orientation (68.9%). Especially TNBC microlobulated mass margins were more more frequent than with ER (+) (2.0%) and HER2 (+) (4.8%) cancers. Conclusions: TNBC have specific characteristic in sonograms. Ultrasonography may be useful to avoid missed diagnosis and false-negative cases of TNBC.