Bacterial Diversity in the Rhizosphere of Halophyte Suaeda japonica in Western and Southern Mudflats of Korea

Park, Suhk-Hwan,Lee, Geon-Hyoung The Ecological Society of Korea 2006 Journal of Ecology and Environment Vol.29 No.4

This study was carried out to investigate the population densities, R/S ratios, and identification of heterotrophic bacteria on the rhizosphere soil of halophyte Suaeda japonica found on the western and southern mudflats of Korea. The population densities of aerobic and anaerobic heterotrophic bacteria on the rhizosphere soil of Suaeda japonica were in the range of $1.3\;{\pm}\;0.3\;{\times}\;10^6\;{\sim}\;6.3\;{\pm}\;3.3\;{\times}\;10^7\;and\;2.8\;{\pm}\;1.3\;{\times}\;10^4\;{\sim}\;1.8\;{\pm}\;0.7\;{\times}\;10^7\;cfu\;g^{-1}\;d.\;wt.$, respectively. In case of physiologically specific bacteria, population densities of amylolytic bacteria on the rhizosphere soil of Suaeda japonica were in the range of $4.4\;{\pm}\;0.6\;{\times}\;10^6\;{\sim}\;2.5\;{\pm}\;1.2\;{\times}\;10^7\;cfu\;g^{-1}\;d.\;wt.$, those of cellulolytic bacteria were from $8.5\;{\pm}\;6.0\;{\times}\;10^4\;{\sim}\;2.3\;{\pm}\;1.6\;{\times}\;10^6\;cfu\;g^{-1}\;d.\;wt.$, and those of proteolytic bacteria were from $3.8\;{\pm}\;1.8\;{\times}\;10^5\;{\sim}\;4.2\;{\pm}\;2.9\;{\times}\;10^6\;cfu\;g^{-1}\;d.\;wt.$, respectively. The R/S ratios were ranged from 2.33 to 2.39. Among eleven isolates from the roots of halophyte Suaeda japonica of Goheung bay by using 16S rDNA analysis, five clones were closely related to ${\gamma}-Proteobacteria$ group and six clones were closely related to ${\alpha}-Proteobacteria$ group. Among four isolates from Suncheon bay, two strains were related to ${\gamma}-Proteobacteria$ group and another two were related to Actinobacteria and Bacilli group, respectively.

삼천포만 인근해역과 저질토에서 종속영양세균과 분변성 기원 세균의 분포

박석환 ( Suhk Hwan Park ),이건형 ( Geon Hyoung Lee ) 한국환경생물학회 2006 환경생물 : 환경생물학회지 Vol.24 No.3

유럽의 유전자변형체 관리규정 : Directive 2001/18/EC를 중심으로

김성환,김나영,박미아,김명희,송석린,임병순 중부대학교 자연과학연구소 2001 自然科學硏究論文集 Vol.10 No.1

Foods derived from Genetically Modified Organisms(GMOs) is the most import issue in the world. To establish safety regulation on foods derived from GMOs, it is needed to review various regulation. So this articles is to summarize the European legislation on GMOs. The objectives of Directive 2001/18/EC is to protect human health and the environment when placing on the market GMOs as or in products within the Community. There are various definition of terms used, such as GMO and environmental risk assessment. In this Directive, 'GMO' means an organism, with the exception of human beings, in which the genetic materials has been altered in a way that does not occur naturally by mating or natural recombination. Principles for the environment risk assessment, information required in the notification and monitoring plan also described in this Directive. Notifier who wants to market GMO shall submit notification to the competent authority according to notification procedure

Bacterial Diversity in the Rhizosphere of Halophyte Phragmites communis at the Western Coastal Mudflats of Korea

Moon, Ho-Sang,Suhk-Hwan Park,가종억,송홍규,Geon-Hyoung Lee* 한국생태학회 2008 Journal of Ecology and Environment Vol.31 No.2

This study investigated the population densities and diversity of heterotrophic bacteria, and the rhizosphere-to-soil ratios (R/S) in the rhizosphere soil of halophyte Phragmites communis at the western coastal mudflats of Korea. The population densities of aerobic heterotrophic bacteria on the rhizosphere soil of P. communis were in the range of 3.3 ± 0.9 × 107~1.2 ± 0.5 × 108 cfu g-1 dry weight (d. wt.). Population densities of amylolytic bacteria ranged from 1.1 ± 0.2 × 106 to 3.0 ± 1.2 × 106 cfu g-1 d. wt., while those of cellulolytic bacteria and proteolytic bacteria ranged from 5.6 ± 2.3 × 106 to 1.5 ± 0.3 × 107 cfu g-1 d. wt. and from 1.4 ± 0.3 × 106 to 3.5 ± 2.3 × 107 cfu g-1 d. wt., respectively. The R/S ratios ranged from 2.26 to 6.89. Genetic (16S DNA) analysis of fifty-one isolates from the roots of P. communis suggested that the dominant species were closely related to the γ-proteobacteria group (18 clones) and the α-proteobacteria group (14 clones). We found that halophyte species and mudflat environment both affected the rhizosphere bacterial communities.

원발성 간암환자에서 간동맥 색전술의 치료효과 - 2. 중간 추적 생존율 -

고윤석(Youn Suck Koh),노임환(Im Hwan Roe),안정경(Jung Kyung Ahn),함준수(Joon Soo Hanm),이민호(Min Ho Lee),기춘석(Choon Suhk Kee),박경남(Kyung Nam Park),이은희(Eun Hee Lee),서흥석(Heung Suk Seo) 대한내과학회 1988 대한내과학회지 Vol.35 No.3

N/A Currently, primary hepatocellular carcinoma (HCC) is the second most common cause of cancer death in men in Korea. Generally, the tumor is unresectable due to underlying liver cirrhosis. As a palliative therapy of HCC, transcatheter arterial embolization (TAE) was performed in 53 patients (57 times) from April 19S5 until December 1986 at Hanyang University hospital, of which the conditions of only 35 patients could be followed after TAE therapy. We have analyzed the effectiveness of TAE therapy on these 35 patients. The results were as follows: In patient who didn't have tumor thrombosis in the first or second portal branches, the mean 8 months survival was 58.2%, while in patients with tumor thrombosis, it was 8.3%. The ratio of the tumor size to the liver measured by a planimeter in hepatic angiography was found to be meaningful in the prognosis; In patients with a ratio below 20%, the 6 months survival rate was 66.7%, and in patients with a ratio of 20% or more, it wis 43.4%, The results suggest that TAE is more beneficial in unresectable HCC which doesn't have tumor thrombosis in the first or second portal branches, and which has a ratio of tumor size to liver below 20% as measured by angiography.

84 - kDa 의 폐렴구균 열충격단백질 CipL 의 Cloning 및 면역특성에 관한 연구

권혁영(Hyog Young Kwon),김용환(Yong Hwan Kim),최혜진(Hye Jin Choi),박연진(Youn Jin Park),표석능(Suhk Neung Pyo),이동권(Dong Kwon Rhee) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.2

Heat shock proteins serve as chaperone by preventing the aggregation of denatured proteins and promote survival of pathogens in harsh environments. In this study, heat shock gene encoding a 84-kDa (p84) protein, which is one of the three major heat shock proteins in S. pneumoniae, was cloned and characterized. PCR with a forward primer derived from N-terminal amino acid sequence of the p84 and a reverse primer derived from the conserved second ATP-binding region of Clp family was used for amplification of the gene encoding the p84 and subsequently the PCR product was used for sequence determination. Sequence analysis of the p84 gene demonstrated that it is a member of ClpL. The deduced amino acid sequence of pneumococcal C1pL shows homology with other members of the Clp family, and particularly, even in variable leader region, with bovine Clp-like protein and L. lactis ClpL. S. pneumoniae clpL is the smallest clp member (701 amono acids) containing the two conserved ATP-binding regions, and hydrophilic N-terminal variable region of pneumococcal Clp ATPase is much shorter than any known Clp ATPases. Histidine tagged ClpL was overexpressed and purified from E. coli. Immunoblot analysis employing antisera raised against pneumococcus p84 demonstrated no cross-reactivity with Clp analog in Eschericha coli, Staphylococcus aureus and human HeLa cells. Preimmunization of mice with ClpL extended mice life partially but did not protect them from death.

군산지역 초등학교 정수기 물의 미생물학적 수질

서란주,박석환,이건형,Seo, Lan-Ju,Park, Suhk-Hwan,Lee, Geon-Hyoung 한국미생물학회 2009 미생물학회지 Vol.45 No.1

2007년 7월부터 12월까지 총 5회 전라북도 군산에 위치한 초등학교의 정수기 물을 대상으로 미생물학적 수질에 대하여 조사하였다. 조사기간 중 정수기 물의 종속영양세균의 분포는 $0{\sim}1.2{\pm}0.2{\times}10^4CFU/ml$의 범주에서 나타났는데, 이 중 최대값은 우리나라 먹는 물 수질기준을 120배 초과하는 값을 나타냈다. 월별 수질기준을 초과하는 정수기 물의 백분율은 7월과 9월에 90%, 10월과 11월에 87.2%, 12월에 93.7%로 조사되었다. 한편 총대장균군과 병원성 세균인 Salmonella와 Shigella는 정수기 물과 수돗물에서 모두 검출되지 않았다. 총 5회의 실험 중 정수기 물에서 분리한 세균을 분자생물학적인 방법으로 동정한 결과, Sphingomonas, Methylobacterium, Caulobacter, Novosphingobium, Bosea, Brevundimonas, Aminobacter, Ralstonia, Mitsuaria, Variovorax, Acidovorax, Massilia, Pseudomonas, Acinetobacter, Aeromonas, Bacillus, Staphylococcus, Brevibacillus, Microbacterium, Lapillicoccus, Micrococcus, Arthrobacter, Janibacter, Flavobacterium, Chryseobacterium, Hymenobacter 등 26개 속이 동정되었다. 검출된 세균의 대부분은 일반 환경에서 다량 존재하는 세균들로서 병원성이 없으나, Pseudomonas fluorescens, Staphylococcus epidermidis, Flavobacterium johnsoniae, Acinetobacter johnsonii 등은 기회감염균으로 면역력이 낮은 초등학교 학생들의 경우 감염의 가능성이 있다. In this research, we investigated the actual conditions of water purification systems at ten elementary schools located in Gunsan, Korea from July to December, 2007. The results were as follows; The population densities of heterotrophic bacteria in water purifiers ranged from 0 to $1.2{\pm}0.2{\times}10^4$ CFU/ml and those of tap water were in the range from 0 to $1.9{\pm}0.3{\times}10^4$ CFU/ml during investigation periods. Ninety percentage of purified water samples in July and September, 87.2% in October and November, and 93.7% in December turned out not to be suitable for drinking. The seasonal variation of the population densities of heterotrophic bacteria from purified waters was not notable. The total coliform, Salmonella and Shigella were not detected in purified water and tap water during investigation periods. Forty-five species of bacteria were isolated from water purifiers. The identified bacterial genera were Sphingomonas, Methylobacterium, Caulobacter, Novosphingobium, Bosea, Brevundimonas, Aminobacter, Ralstonia, Mitsuaria, Variovorax, Acidovorax, Massilia, Pseudomonas, Acinetobacter, Aeromonas, Bacillus, Staphylococcus, Brevibacillus, Microbacterium, Lapillicoccus, Micrococcus, Arthrobacter, Janibacter, Flavobacterium, Chryseobacterium, and Hymenobacter: Among the isolates, opportunistic pathogens such as Pseudomonas fluorescens, Staphylococcus epidermidis, Flavobacterium johnsoniae, and Acinetobacter johnsonii were also found.

간장 ( 肝臟 ) 및 담도 ( 膽道 ) : 만성 간염 환자에 있어서의 Ursodeoxycholic Acid ( SGD - FR ) 의 임상 효과

함준수(Joon Soo Hahm),이동후(Dong Hoo Lee),기춘석(Choon Suhk Kee),박경남(Kyung Nam Park),양석철(Suck Chul Yang),김태환(Tae Hwan Kim),이종철(Chong Chul Lee) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.4

N/A Randomized double blind trials have done to determine the effect of ursodeoxycholic acid (SGD-F) to the progress of the chronic hepatitis, 30 patients who had been admitted at the department of internal medicine, Hanyang University Hospital from October 1989 to March 1990 were selected to the study; UDCA was given to 15 patients and placebo was given to the other 15 patients. The results are as follows; 1) Clinical stage showed improvement (14 cases; 93.3%) and serum transaminase was decreased markedly after UDCA (SGD-F) treatment. 2) Serum transaminase (SGOT, SGPT) was more decreased in UDCA treatment group than in placebo treatmelnt group. 3) The UCDA (SGD-F) treatment group showed more clinical improvement than the placebo treatment group. In conclusion, the ursodeoxycholic acid (SGD-F) treatment may be effective for patients with chronic liver disease.

Evaluation of Near-infrared Fluorescence-conjugated Peptides for Visualization of Human Epidermal Receptor 2-overexpressed Gastric Cancer

Jeong, Kyoungyun,Kong, Seong-Ho,Bae, Seong-Woo,Park, Cho Rong,Berlth, Felix,Shin, Jae Hwan,Lee, Yun-Sang,Youn, Hyewon,Koo, Eunhee,Suh, Yun-Suhk,Park, Do Joong,Lee, Hyuk-Joon,Yang, Han-Kwang The Korean Gastric Cancer Association 2021 Journal of gastric cancer Vol.21 No.2

Purpose: A near-infrared (NIR) fluorescence imaging is a promising tool for cancer-specific image guided surgery. Human epidermal receptor 2 (HER2) is one of the candidate markers for gastric cancer. In this study, we aimed to synthesize HER2-specific NIR fluorescence probes and evaluate their applicability in cancer-specific image-guided surgeries using an animal model. Materials and Methods: An NIR dye emitting light at 800 nm (IRDye800CW; Li-COR) was conjugated to trastuzumab and an HER2-specific affibody using a click mechanism. HER2 affinity was assessed using surface plasmon resonance. Gastric cancer cell lines (NCI-N87 and SNU-601) were subcutaneously implanted into female BALB/c nu (6-8 weeks old) mice. After intravenous injection of the probes, biodistribution and fluorescence signal intensity were measured using Lumina II (Perkin Elmer) and a laparoscopic NIR camera (InTheSmart). Results: Trastuzumab-IRDye800CW exhibited high affinity for HER2 (K_D=2.093(3) pM). Fluorescence signals in the liver and spleen were the highest at 24 hours post injection, while the signal in HER2-positive tumor cells increased until 72 hours, as assessed using the Lumina II system. The signal corresponding to the tumor was visually identified and clearly differentiated from the liver after 72 hours using a laparoscopic NIR camera. Affibody-IRDye800CW also exhibited high affinity for HER2 (K_D=4.71 nM); however, the signal was not identified in the tumor, probably owing to rapid renal clearance. Conclusions: Trastuzumab-IRDye800CW may be used as a potential NIR probe that can be injected 2-3 days before surgery to obtain high HER2-specific signal and contrast. Affibody-based NIR probes may require modifications to enhance mobilization to the tumor site.

바이칼호에서 분리한 빈영양성 세균과 저온성 세균의 탄소원 이용 특성

이건형,배명숙,박석환,송홍규,안태석,Lee Geon-Hyoung,Bae Myoung-Sook,Park Suhk-Hwan,Song Hong-Gyu,Ahn Tae-Seok 한국미생물학회 2004 미생물학회지 Vol.40 No.3

2000년 9월부터 2002년 12월 사이에 바이칼호에서 분리된 빈영양성 세균 168균주와 저온성 세균 132균주를 대상으로 BIOLOG Microplate를 이용하여 탄소원의 이용특성을 조사하였다. 본 연구에 사용된 빈영양성 세균 중 oxidase 양성 (GN-NENT 그룹)의 86% (56균주)와 oxidase음성 (GN-ENT그룹)의 89% (92균주), 저온성 세균 중 oxidase 음성 (GN-ENT 그룹)의 82% (85균주)는 다앙한 탄소원 중에서 $\alpha$-D-glucose를 이용할 수 있었으며, 저온성 세균 중 oxidase 양성 (GN-NENT 그룹)의 93% (26 균주)는 bromosuccinic acid를 이용하였다. $\alpha$-D-lactose는 빈영양성 GN-ENT 그룹의 일부만이 이용하였으며 나머지 균주들은 전혀 이용하지 못하였다. BIOLOG Microplate를 이용하여 동정된 균들을 속별로 살펴보면, Pseudomonas속이 49균주로 가장 많았으며, 그 외에도 Salmonella 속, Serratia속, Buttiauxella 속, Pantoea 속, Yersinia 속, Brevundimonas 속, Hydrogenophaga 속, Photorhabdus 속, Sphingomonas 속, Xenorhabdus 속이 동정되었다. To scrutinize the physiological diversity by BIOLOG microplate, the carbon source utilization patterns of 168 strains of oligotrophic bacteria and 132 strains of psychrotrophic bacteria isolated from Lake Baikal during 2000 and 2002 were investigated. Eighty-six percent (56 strains) of oxidase test positive group (GN-NENT group) and 89 % (92 strains) of oxidase test negative group (GN-ENT group) among oligotrophic bacteria, and 82% (85 strains) of oxidase test negative group among psychrotrophic bacteria were able to utilize $\alpha$-D-glucose as a sole-carbon-source, and 93% (26 strains) of oxidase test positive group among psychrotrophic bacteria were able to utilize bromosuccinic acid as a sole-carbon-source. However, most strains except few oligotrophic bacteria with oxidase test negative group were not able to utilize $\alpha$-D-lactose as a sole-carbon-source. Most dominant genus among 300 strains was Pseudomonas (49 strains). Other dominant genera belonged to Salmonella, Serratia, Buttiauxella, Pantoea, Yersinia, Brevundimonas, Hydrogenophaga, Photorhabdus, Sphingomonas, and Xenorhabdus. Our results by BIOLOG identification system were able to provide basic data to determine community-level carbon source utilization patterns and to accomplish the efficient and reliable identification for microbial community structure in Lake Baikal.