1-Deoxynojirimycin의 급성독성 및 항균효과

백남수,김영만 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.6

Streptomyces exofoliatus SID9135 균주의 발효배양 여액으로부터 anion(Dowex 1X2-100 Cl^-) 및 cation(Dowex 50X4-100 H^+) 이온교환수지, Sephadex LH20 컬럼, TSK 겔 컬럼크로마토그래피 등으로 저해물질을 정제한 후, 최종적으로 HPLC를 사용하여 α-glucosidase 저해물질인 1-deoxynojirimycin을 순수 분리정제 하였다. IRC 마우스에서의 1-deoxynojirimycin의 급성독성 조사에서 200㎎/㎏의 dose로 경구 투여하여 10일간 관찰하였을 때 치사동물이 전혀 없었으며 체중증가는 대조군과 동일한 양상을 보였다. Agar 희석법으로 20종의 병원균에 대한 1-deoxynojirimycin과 AO-128 및 acarbose의 MIC한 결과 1-deoxynojirimycin과 대조약물인 AO-128 및 acarbose의 농도가 100㎍/ml 이상에서도 20종의 시험균주 모두에 대해 항균효과를 나타내지 않았다. 1-Deoxynojirimycin which is a potent intestinal α-glucosidase inhibitor was purified from the culture broth by ion exchange chromatography, Sephadex LH20 column chromatography, TSK gel chromatography and HPLC respectively. Acute toxcity of 1-deoxynojirimycin, which was loaded through the oral as dose of 200㎎/㎏, was investigated in IRC mouse. None of the tested IRC mice were not dead and increase of body weight showed also the same results in comparison with control mice. The antimicrobial susceptibility of 20 pathogenic strains against 3 antidiabetic compounds (1-deoxynojirimycin, AO-128, acarbose) were obtained by agar dilution method. All of the three antidiabetic compounds had very weak antimicrobial activity (MIC>100 ㎍/ml).

프로축구 선수들의 성취목표 지향성과 자기실현의 관계

백남섭,김현수 龍仁大學校 體育科學硏究所 2003 體育科學硏究論叢 Vol.13 No.2

The purpose of this study is to see how a soccer player's performance orientation affects his/her self fulfillment. To this end, I analyzed 1) the relation between performance orientation and self fulfillment by taking into account sociodemographic characteristics and the effect of performance orientation on the degree of self fulfillment. Simple random sampling was performed to produce a sample of 250 players selected from those who registered with the K-League in 2002. 16 questionnaires deemed either untrustworthy or partially omitted were excluded, leaving 234 questionnaires for study. The questionnaire contained total 33 questions (i.e., 3 on general characteristics, 20 on performance orientation, and 9 on self fulfillment). Frequency analysis, t-test, Oneway-ANOVA, Scheffe test, correlation analysis and regression analysis were performed. The above methods and procedures produced the following results. First, training and order, which are sub-variables for performance orientation, had a statistically significant difference (5% range) regarding age while assignment orientation showed a statistically significant difference (5% range) with regard to education level. Second, goal realization, which is a sub-variable for self fulfillment, had a statistically significant difference (5% range) regarding age and education level. Thud, competence demonstration, which &d a significant effect on self orientation and training/order, showed 27.9%(R^(2)=.279) of explicability with regard to self fulfillment. Fourth, competence development, which had a significant effect on assignment orientation and training/order, resulted in 49.5%(R^(2)=.495) of explicability regarding self fulfillment. Fifth, goal realization, which had a significant effect on all variables including assignment orientation, self orientation and training/order, resulted in 40.2%(R^(2)=.402) of explicability regarding self fulfillment.

프로축구 관중의 공정성 지각과 고객만족 구매행동의 인과관계

백남섭,김태수 龍仁大學校 體育科學硏究所 2008 體育科學硏究論叢 Vol.17 No.1

Service fairness in the aspect of customer-relation centered management in the service business have become a very important analytical factor. The purpose of this study was to investigate the cause-effect relationship between the service fairness that perceived by customers based on the service fairness theory under the environments of a pro soccer, and by using such customer satisfaction as parameters, it is also to identify casualty of the purchase behaviors in between, which are subsequent actions of customers, so then it will be meaningful to provide these basic reference materials that may be of great use in establishing marketing strategy. This study established causal relationship model concerned with service fairness, customer satisfaction, and purchase behavior on the ground of the results of precedent researches and hypothetical models, then after, I examined the cause-effect relationship between variables through means of structural equation model. The subjects of this research were the clients who are using the pro soccer game, and 430 subjects were sampled through convenience sampling method. Data were analyzed through frequency analysis and exploratory factor analysis using SPSS 13.0, also analyzed through confirmatory factor analysis and covariance structure analysis using AMOS 5.0 program. Results revealed from these research methods and analyses of data are as follows: First, service fairness had significant influences on customer satisfaction. Second, customer satisfaction affected purchase behavior significantly. Third, direct effect of service fairness on the customer satisfaction was revealed as of 1.031, and the direct effect of customer satisfaction on the purchase behavior was revealed as of .593. Also, the indirect effect that influenced on purchase behavior through customer satisfaction resulted in .611.

Streptomyces sp. NS15 배양액에 의한 α-Glucosidase 저해

백남수,김영만 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.6

Streptomyces sp. NS15 균주로부터 비단백질성 α-glucosidase 저해제의 생산을 위해 초기 배지의 최적 pH와 질소원, 탄소원, 금속 이온, 교반속도 및 통기량 등의 효과에 대하여 조사하였다. 배지의 초기 최적 pH는 7.0이었으며 가장 효과적인 질소원과 탄소원은 각각 soybean meal 2.0%(w/v)와 glucose 1.6%(w/v)였으며 금속이온에서는 Fe^+2에서 저해활성에 있어서 약간의 상승효과를 나타낸 반면 Cu^+2은 오히려 활성저하를 보였다. Jar fermentor 배양에서의 교반속도와 통기량은 각각 400rpm과 1vvm에서 효과적이었으며 최적 배지와 최적조건 하에서 4일간 배양한 배양여액의 저해활성이 돼지 소장의 α-glucosidase에 대해 3,200units/ml로 기본배지(CYM)에서 보다 25배 높은 저해활동을 보였고 5일째부터는 저해활성이 점차 감소하는 양상을 보였다. For the production of nonprotein α-glucosidase inhibitor from the Streptomyces sp. NS15 strain, effects of initial optimum pH, nitrogen sources, carbon sources, cationic metal ions, agitation speed and aeration rate were investigated. Initial optimum pH of medium was 7.0. The most effective nitrogen and carbon source were soybean meal 2.90%(w/v) and glucose 1.6%(w/v), respectively. The cationic metal ions had no stimulating effect on inhibitory activity of α-glucosidase except Fe^2+. Agitation speed and aeration rate were effective at 400rpm and 1vvm, respectively. In the jar-fermenter cultivation for 4 days under optimal culture conditions, the culture broth showed the inhibitory activity of 3,200units/ml, which is 25 times higher than that of basic medium(CYM) for porcine intestinal α-glucosidase. The inhibitory activity of α-glucosidase reached about 3,200units/ml after 4 days of cultivation and decreased gradually for a further two days.

수도 계량기 검침 자동화 시스템 구현

백남수,이은주 大田産業大學校 2002 한밭대학교 논문집 Vol.19 No.-

In this paper, we proposed a new method for automatic inspection of water gauge, and implemented it. We decided meter area for inspection using intensity of input image, and noise removed by labeling and size of label. The numbers were overlapped or not, it was decided by characteristics of numbers, and recognition area was decided by size of area. Finally, we computed the similarity between area of numbers and reference pattern by double template matching, and the number that similarity is the highest with area of numbers was result of recognition.

VDT작업자에서 업무로 인한 정신사회적 스트레스에 대한 인지가 근골격계장애에 미치는 영향

백남종,하미나,조수헌,강대희,백도명,주영수,윤덕로,권호장 大韓産業醫學會 1996 대한직업환경의학회지 Vol.8 No.3

업무에 의한 정신사회적 스트레스가 근골격계 질환의 유병에 미치는 영향을 파악하기 위하여 전화안내원을 대상으로 단면적 조사연구를 수행하였다. 연구 결과 나이, 교육정도, 근무연한, 가사부담 등의 인구학적 변수는 근골격계질환의 유병에 영향을 미치지 않는 것으로 분석되었다. 정신사회적 스트레스 중 업무요구도가 높다고 느끼는 집단에서 그렇지 않은 집단에 비해 근골격계 질환의 유병률이 통계적으로 유의하게 높게 나타났으나 업무재량도나 사회적지지도는 근골격계질환과 유의한 상관관계를 발견할 수 없었다. 그러나 업무요구도는 높고 업무재량도는 낮다고 느끼는 '근무긴장도가 가장 높은 군'은 업무요구도는 낮고 업무재량도는 높다고 느끼는 '근무긴장도가 낮은 군'에 비해 근골격계 질환의 발생위험이 통계적으로 유의하게 높은 것으로 나타났다. 본 연구의 결과는 업무로 인한 정신사회적 스트레스에 대한 인지 정도에 따라 근골격계질환의 유병률이 달라짐을 시사하고 있다. 따라서 작업관련성 근골격계질환의 예방을 위해서는 기존의 인간공학적 접근 이외에 스트레스를 감소시키려는 노력이 동시에 진행되어야 할 것으로 생각한다. A cross-sectional study was conducted to assess the association between perceived occupational psychosocial stress at work and the work-related musculoskeletal disorder(WRMSD) among employees using video display terminals. The study included 111 female telecommunication employees from three companies at ChungBuk province. Cases of WRMSD were defined using symptom questionnaire and physical examination conducted by rehabilitation specialist. Information on demographics, individual factors, and perceived psychosocial stress, were obtained by self-administered questionnaire. For assessing perceived psychosocial stress, we used variables from 'job strain model' proposed by Karasek. Associations between perceived psychosocial stress and WRMSD were assessed by multiple logistic regression models. Forty nine(44% to the total) subjects met our operational definition for WRMSD. Age, seniority, housing load were not associated with WRMSD. Perceived psychosocial stress was associated with WRMSD[odds ratio=3.28, 95% confidence interval: 1.05-10.19]. This study suggests that perceived occupational psychosocial stress is related to the prevalence of WRMSD.

강릉시내 자전거 전용도로개설의 타당성에 관한 설문조사 분석

정문선,백남훈,이창수,윤신 강릉대학교동해안지역연구소 1994 東海岸硏究 Vol.5 No.1

본 논문에서는 강릉시내에서의 자전거 전용도로 개설에 관한 타당성을 조사하기 위햐여 지역주민을 대상으로 설문조사를 실시하였으며 그 결과를 분석하였다. 설문내용에는 현재 자전거 이용실태, 자전거 전용도로 개설의 타당성, 노선등을 포함하였다. 설문조사결과 자전거 전용도로 개설에 관해 긍정적 답변이 많았다. We make up the questionaire for the validities of establishing the bicycle-way in Kangnung, and analyze the results. The contents of the questionaire include the research on actual state of using one's own bicycle, the validities of establishing the bicycle-way, and the routes. The results of the questionaire show the affirmative answer for the establishing the bicycle-way in Kangnung.

유포자성 유산균을 함유한 홍삼양갱의 개발에 관한 연구

윤광선,백남수,김영만 안성산업대학교 2006 論文集 Vol.38 No.-

유포자성 유산균인 Lactobacillus sporogenes는 혐기 조건으로는 젖산 발효율 97%로 homolactic acid 발효를 하였고 호기 조건으로는 배양결과 생균수 20 × 10^(8)CFU/mL로 포자형성율도 97%를 얻었다. 또한 유포자성 유산균을 이용한 홍삼양갱을 제조하기 위하여 홍삼양갱의 반제품 1kg의 끓인액의 80℃에서 유포자성 유산균을 첨가{20g (spore수 200 × 10^(8) CFU/g)} 제조한 결과 {1.4 × 10^(8) CFU/g (유포자성 유산균 원말 20g/홍삼양갱 1kg)} 제조 후의 생존율이 70%를 유지하면서 끓인 홍삼양갱 제품에 이용 가능성이 높게 나타났다. 이 제품을 건강식품으로 실용후의 결과를 조사하기 위하여 인공위액(pH 3.0)에 대한 내산성을 조사한 결과 4시간 후에 생존율 50%로 나타났다. 그리고 실온에서 1년간 보존시험결과 생존율이 75% 이상으로 저장성이 우수하다고 판단된다. 이상의 결과에서 볼 때 유포자성 유산균을 함유한 홍삼양갱 등의 건강 기능식품의 개발 가능성이 있다고 사료된다. In order to develop red Genseng Thick Jelly containing lactic acid bacteria. Lactobacillus sporogenes spore which has resistance in heat and low pH was used. Jar fermentor culture of Lactobacillus sporogenes under aerobic condition was showed that the number of spore reached about 20×l0^(8)CFU/㎖ and sporulation rate was 97% and lactic acid production yield was 97% under anaerobic condition. The survival rate of Lactobacillus sporogenes spore showed 70% in the product after addition in 80℃ hot jelly solution. In the treatment of artificial gastric juice(pH 3.0) for 4 hours, the survival rate was 50%. It's storage stability was 70% during the 12 months at room temperature. These results suggest that red Genseng Thick Jelly containing lactic acid bacteria play a role as functional food.

유포자성 유산균을 이용한 라면스프의 개발에 관한 연구

정호동,백남수,김영만 안성산업대학교 2006 論文集 Vol.38 No.-

L. sporogenes를 호기조건으로 24시간 배양한 결과 생균수는 20×10^(8) CFU/mL로 나타났으며 포자 형성율은 97%를 보였다. 라면 스프 및 조리시간에 따른 생균수를 측정한 결과 3회사 제품 모두 약간의 근소한 차이는 있지만 조리 후 10분이 지나 72~74℃ 가 될 때 까지는 생존율이 약 10%씩 감소하다가 조리 후 10분이 지나 용기면의 온도가 60℃ 범위를 유지하는 동안은 생존율이 75~78%를 유지하면서 그 이후 생존율에 거의 변화를 보이지 않았다. 인공위액에 3사의 컵라면 스프를 각각 3.2% 첨가한 후 포자의 생존율은 30분경과 후 대조군은 95%를 나냈으며 N사와 H 및 S사는 각각 90%, 88%, 91%를 나타내어 대조군과 큰 차이를 보이지 않았다. 1.0% 농도의 Oxgall에 3사의 컵라면 스프를 각각 3.2% 첨가한 후 생존율을 측정한 결과 60분경과 후 에서는 대조군이 95%, B사와 N 및 S사 가 각각 82%, 80%, 80%로 약 15% 이상 차이가 나타났으며 60분경과 후 에는 대조군이 90% 의 생존율을 보인 반면 B사와 N 및 S사는 각각 70%, 68%, 67%로 대조군 보다 20~23% 사멸 율이 증가하였다. 스프에 함유된 포자에 대하여 20℃와 37℃에서의 저장성 시험결과 20℃에서 보존 한 경우 N사의 스프에시 3, 6, 9개월 후의 생존율이 94, 90, 84%였으며 37℃에서 보존 한 경우 20℃에서 보존 한 경우 보다 20%정도 감소율이 증가하여 온도에 의한 영향이 매우 큰 것으로 나타났다. In order to develop functional cup ramyun containing with spore forming lactic acid bacteria powder, Lactobacillus sporogenes which has resistant spore against high temperature, low pH and oxgall was selected. In jar fermentor experiment under optimal culture condition, the number of spore of Lactobacillus sporogenes reached about 20×10^(8) cfu/ml and sporulation rate was about 97%. In the treatment of artificial gastric juice(pH 3.0) with cup ramyun soup for 30min, the survival rate of spores in B, N and S company soup were about 90%, 88% and 91% respectively. Survival rate in the presence of 1.0% oxgall for 1 hour had more than 80%. The storage stability was more than 70% at 20℃ and 60% at 37℃, respectively.

곰팡이에서 분리한 Helicobacter pylori 항균물질의 분리 및 구조규명

남궁준,연승우,백남수,김태한,김영호,김창진,김기원 충남대학교 약학대학 의약품개발연구소 1998 藥學論文集 Vol.14 No.-

Helicobacter pylori is a Gram-negative bacterium which causes chronic gastritis and is associated with gastric ulcer, duodenal ulcer and gastric carcinoma. In the process of screening of antibacterial activities against H. pylori from soil microorgani는, fungus No. 60686 was isolated. After fementaion of No. 60686, the antibacterial compound was isolated, purified and followed by extraction of mycelium with orgainic solvents, acetone and ethyl acetate, through silica gel chromatography, LH-20 gel chromatography and HPLC. As a result of the structural analyses of the compound by IR, ^1H- and ^13C-NMR, FAB/Mass spectrophotomer, the compound having the antimicrobial activity was idenfitied as chaetoglobosin A (C_32H_36N_2O_5), a cytochalasan derivative. The antimicrobial activity of chaetoglobosin A was tested against Gram-positive and negative bacteria by paper disk method. Among the test strains of 9 Gram-positive bacteria and 18 Gram-negative bacteria containing 4 H. pylori strains, the growth of 4 H. pylori strains and 3 S. aureus strains (SG 511, 285 and 503) was only inhibited by chaetoglobosin A. Also it was shown that its growth inhibition against H. pylori strains was stronger than that against S. aureus strains at the treatment of the same concentration. Therefore it was concluded that chaetoglobosin A has a specific growth inhibition against H. pylori of the tested bacteria.