Dietary methionine supplementation to a low-protein diet improved hair follicle development of Angora rabbits

Zhao Man,Wang Tongtong,Wang Bin,Liu Chuanhua,Li Fuchang,Liu Lei 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.6

Objective: Angora rabbits fed a low-protein diet exhibit decreased hair production performance. This study was set out to evaluate the effects of methionine on hair properties and nitrogen metabolism in Angora rabbits fed a low-protein diet and to investigate the gene expression related to hair follicle development to determine the possible molecular mechanism of methionine effects on hair follicle development. Methods: An experiment was conducted to investigate the effects of DL-methionine addition on a low-protein diet on hair development in Angora rabbits. Angora rabbits were divided into 5 groups: fed a normal diet (control), fed a low-protein diet (LP), or fed an LP supplemented with 0.2%, 0.4%, or 0.6% DL-methionine (Met). Results: The results showed that rabbits in the LP group had lower wool yield than the control rabbits, but the addition of 0.4% to 0.6% Met to LP attenuated these effects (p< 0.05). Dietary addition of 0.4% to 0.6% Met to LP increased the apparent nitrogen digestibility, nitrogen utilization rate, and feed efficiency (p<0.05). Feeding LP decreased the insulinlike growth factor 1 (IGF1), keratin-associated protein (KAP) 3.1, and KAP 6.1 mRNA levels compared with the control, but the addition of 0.4% Met in LP attenuated these effects (p<0.05). Relative to the LP or control group, dietary addition of 0.4% Met increased versican mRNA levels. Conclusion: In conclusion, the addition of Met to LP could improves wool production performance and feed efficiency and reduce nitrogen emissions in Angora rabbits. Met can promote hair follicle development, which may be associated with IGF1, KAP, and the versican signaling.

Micro-grinding temperature prediction considering the effects of crystallographic orientation and the strain induced by phase transformation

Man Zhao,Xia Jinan,Steven Y. Liang 한국정밀공학회 2019 International Journal of Precision Engineering and Vol.20 No.11

This paper proposes a physical-based model to predict the temperature in the micro-grinding of maraging steel 3J33b with the consideration of material microstructure and process parameters. In micro-grinding, the effects of crystallography on the grinding machinability become significant, since the depth of cut is of the same order as the grain size. In this research, the Taylor factor model for multi-phase materials is proposed to quantify the crystallographic orientation (CO) with respect to the cutting direction by examining the number and type of activated slip systems. Then, the flow stress model is developed, in which both the athermal stress resulted from the COs and the strain induced by the phase transformation are taken into account. On the basis of the flow stress model, the grinding forces are predicted followed by the calculation of the grinding heat. In the investigation, the triangular heat flux distribution and the reported energy partition model are applied in the calculation of workpiece temperature. Furthermore, the temperature model is validated by conducting an orthogonal-designed experiment, with the predictions of the maximum temperature in good agreement with the experimental data. Moreover, the predictive data is compared with the predictions resulted from the two other previously reported models. The results indicate that the proposed temperature model with considering the effect of CO and the phase transformation improved the prediction accuracy of the micro-grinding temperature.

Preparation, characterization and application of poly(sodium p-styrenesulfonate)/poly(methyl methacrylate) particles

Man Zhang,Rui Wang,Tao Xiang,Wei-Feng Zhao,Chang-Sheng Zhao 한국공업화학회 2016 Journal of Industrial and Engineering Chemistry Vol.34 No.-

In this study, poly(sodium p-styrenesulfonate)/poly(methyl methacrylate) particles were facilelyprepared by in situ cross-linked polymerization followed with a liquid–liquid phase inversion technique. The particles exhibited selective adsorption for cationic dyes due to the negatively charged sulfonicgroups. The intra-particle diffusion process was the rate-limiting step for the adsorption of methyleneblue. More than 90% of cationic dye was removed by the adsorption column of the particles after threecirculations. The particles could be facilely fabricated and industrially used for wastewater treatment.

Transcriptional profiling analysis of OsDT11-mediated ABA-dependent signal pathway for drought tolerance in rice

Zhao Man,Ju Yanhu,Zhao Bo,Li Xiaoming,Dai LIli,Qu Jicheng,Chu Zhaohui,Ding Xinhua 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.5

Small cysteine-rich peptides (CRPs) are important in plant responses to biotic and abiotic stresses. OsDT11, encoding an 88 amino acid CRP-type protein, has been reported to enhance tolerance to drought in rice (Oryza sativa L.) without negatively afecting other agronomic traits. However, the molecular mechanisms of OsDT11-mediated drought tolerance are still unclear. Here, we performed RNA-Seq analysis to compare the transcriptome profles between wild-type (WT) and OsDT11-overexpressing (OE) rice lines under drought stress or under control (non-drought) conditions. A total of 1570 and 1421 diferentially expressed genes were identifed in the OE lines and the WT under drought treatment, respectively, compared to non-drought conditions. Gene ontology analysis indicated that the 430 up-regulated genes in common to both OE and the WT lines were induced for functions related to responses to water deprivation and to abscisic acid (ABA). More than half of these genes had higher expression in the OE than in the WT under drought stress. In the OE, but not in the WT, 294 genes were specifcally up-regulated under drought stress and were functionally enriched in starch and sucrose biosyn�thetic processes and in response to stress. This implies that OsDT11 not only triggers strongly response to drought stress, but also alters several metabolic processes to enhance drought tolerance. Gene expression profling suggests that OsDT11 confers drought tolerance by mediating an enhanced response to drought stress in an ABA-dependent signaling pathways.

사상 : 맹자여아리사다덕정치윤리관지비교(孟子與亞里士多德政治倫理觀之比較)

조만해 ( Man Hai Zhao ) 온지학회 2004 溫知論叢 Vol.11 No.-

Using three-dimensional theory of elasticity for vibration analysis of laminated sectorial plates

Liyuan Zhao,Man Wang,Rui Yang,Meng Zhao,Zenghao Song,N. Bohlooli 국제구조공학회 2023 Steel and Composite Structures, An International J Vol.48 No.1

The main goal of this paper is to study vibration of damaged core laminated sectorial plates with Functionally graded (FG) face sheets based on three-dimensional theory of elasticity. The structures are made of a damaged isotropic core and two external face sheets. These skins are strengthened at the nanoscale level by randomly oriented Carbon nanotubes (CNTs) and are reinforced at the microscale stage by oriented straight fibers. These reinforcing phases are included in a polymer matrix and a three-phase approach based on the Eshelby-Mori-Tanaka scheme and on the Halpin-Tsai approach, which is developed to compute the overall mechanical properties of the composite material. Three complicated equations of motion for the sectorial plates under consideration are semi-analytically solved by using 2-D differential quadrature method. Using the 2-D differential quadrature method in the r- and z-directions, allows one to deal with sandwich annular sector plate with arbitrary thickness distribution of material properties and also to implement the effects of different boundary conditions of the structure efficiently and in an exact manner. The fast rate of convergence and accuracy of the method are investigated through the different solved examples. The sandwich annular sector plate is assumed to be simply supported in the radial edges while any arbitrary boundary conditions are applied to the other two circular edges including simply supported, clamped and free. Several parametric analyses are carried out to investigate the mechanical behavior of these multi-layered structures depending on the damage features, through-the-thickness distribution and boundary conditions.

Application of Slow-Freezing Cryopreservation Method for the Conservation of Diverse Potato (Solanum tuberosum L.) Genotypes

Zhao Mei-Ai,Dhital Shambhu P.,Fang Yi-Lan,Khu Dong-Man,Song Ye-Su,Park Eung-Jun,Kang Chang-Won,Lim Hak-Tae The Korean Society of Plant Biotechnology 2005 Plant molecular biology and biotechnology research Vol.7 No.3

Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. In vitro plantlets of the potato genotypes of 'Atlantic', 'Superior’, 'Namseo', 'J138', and 'CTO5-5' were cold acclimated, and the excised axillary buds were precultured, osmoprotected, exposed to plant vitrification solution, frozen slowly to $-40^{\circ}C$ and then rapidly plunged into liquid nitrogen, thawed and finally plated on the regeneration medium. It was found that the higher the sucrose concentrations in the subculture medium of donor plantlets, the higher the survival rates of shoot tips after cryopreservation, and the highest survival (20%) was observed in the medium added with 0.25 M sucrose. As for the effect of cooling, $0.3^{\circ}C/min$ cooling speed showed the highest survival (25%). Different varieties showed different responses over different cryopreservation treatments. Survival rate was increased by slow-freezing technique method as compared with that of the basic cryopreservation method of vitrification alone in the diverse potato genotypes. Leaf and tuber morphologies of potatoes regenerated after cryopreservation using slow freezing technique were similar to those derived from the in vitro stock plantlets.

Suppression of synuclein gamma inhibits the movability of endometrial carcinoma cells by PI3K/AKT/ERK signaling pathway

Man Ni,Yue Zhao,Xiaoguang Wang 한국유전학회 2021 Genes & Genomics Vol.43 No.6

Background Although overexpression of synuclein gamma (SNCG) has been reported in several cancers, few studies have been performed onSNCG in endometrial carcinomas. Objective This study aimed to investigate the role of SNCG in the progression of endometrial carcinoma. Methods The expression pattern and function ofSNCG gene were analyzed using the Gene Expression Omnibus (GEO) and Gene Set Enrichment Analysis (GSEA) datasets. Two vector types, containing either SNCG or negative control shRNAs, were used to evaluate cell proliferation, apoptosis, and metastasis using Cell Counting Kit 8, colony formation, fow cytometry, wound-healing, transwell, and invasion assays. The relative protein levels of N-cadherin, E-cadherin, vimentin, p-PI3K, PI3K, p-AKT, AKT, p-ERK, and ERK were determined by western bloting. Results Our results revealed thatSNCG mRNA expression and SNCG protein levels in shRNA-treated SPEC2 cells were lower than in the negative control cells. Furthermore, cell proliferation, migration, and invasion were signifcantly inhibited in SNCG shRNA-treated cells, but apoptosis was increased. The results of western blot analysis indicated that SNCG silencing reduced the protein levels of N-cadherin, vimentin, p-PI3K, p-AKT, and p-ERK, but not those of total PI3K, AKT, and ERK. Conclusions Therefore, shRNA-mediated suppression of SNCG inhibited SPEC2 cell proliferation, migration, and invasion, and promoted SPEC2 cell apoptosis, which was presumably accomplished via regulation of the PI3K/AKT/ERK signaling pathway.

Adsorption behavior and mechanism of multiple Mg atoms on the surface of AlNi compound at Mg alloy/steel interface

Zhao Shuang,Song Gang,Chen Jian,Yao Man,Liu Liming 한국물리학회 2022 Current Applied Physics Vol.33 No.-

The interface between Mg alloy and the intermetallic compounds (IMCs) is the weak point during the welding of Mg alloy and steel. The models of AlNi surface under different number of absorbed magnesium atoms were established. The absorption energy and electronic properties of the Nth (N represents the number of Mg atoms, which may be 1, 2, 3, and 4 in this paper) Mg atom on the AlNi surface were calculated using first-principles calculations. The results show that the previously absorbed Mg atoms will promote the absorption of the latter Mg atom when the distance between them is equal or less than 2.89 Å. The absorption energy of the Mg atoms on the AlNi surface is related to the electronic exchange between different atoms. The results will provide a new perspective for understanding the atomic scale mechanism of the bonding between the Mg atom and AlNi IMCs.

A Technique and software of analysis and control for measurement process

(Zhao Feng Yu),(Bo Berg Man) 한국품질경영학회 2000 The Asian Journal on Quality Vol.1 No.1

In this paper, a two-section method for measuring is introduced and the variation sources of measurement process are analysed. Measuring is a special process in general process. Various variation source must be firstly decomposed so that the statistical distribution law of measuring process can be established, and then implement monitoring control of the measuring process. A special method to obtain the measuring variation is discussed, and a monitoring control technique for measuring process is studied based statistical distribution. Towards the end, we briefly introduce software design for the analysis and control of a measurement process.