Provision of a protein-rich supplement for grazing suckling female beef calves to improve productive performance and metabolic response

Moreno Deilen S,Ortega Román M,Marquez David C,Moreira Thiago R,Santos Edson J dos,Almeida Daniel M de,Paulino Mário Fonseca,Rennó Luciana Navajas,Detmann Edenio 아세아·태평양축산학회 2022 Animal Bioscience Vol.35 No.8

Objective: This study was conducted to evaluate the effects of the provision of a proteinrich supplement on productive performance, and metabolic profile on grazing suckling female beef calves in tropical conditions during 150 d of experimentation. Methods: Fifty-six Nellore suckling female calves, and their respective dams were distributed in a completely randomised design and made to undergo two treatments as follows: UNS (without supplementation), and SUP (supplementation with 5 g/kg body weight [BW] of a protein supplement). Throughout the experiment, animal performance and metabolic profile were evaluated. Also, ureagenesis and gluconeogenesis were assessed for gene expression. Results: SUP female calves showed a higher voluntary intake (p≤0.03) of the diet components evaluated, digestibility of organic matter (p≤0.02) and microbial nitrogen production (MICN; p≤0.02) compared to UNS female calves. In its turn, serum urea nitrogen (p≤0.01) and insulin-like growth factor-1 (p≤0.03) levels and ureagenesis (p≤0.04) increased in SUP female calves compared to UNS female calves. Blood glucose and triglyceride levels were not affected by supplementation. The average daily gain (ADG) from SUP female calves was higher (p≤0.02) compared with UNS female calves. However, supplementation did not affect the body measures of the animals. Conclusion: In summary, provision of a protein-rich supplement improves the intake and nutrients digestibility, ADG and final BW and increases metabolic indicators of the protein status in grazing suckling female beef calves in tropical conditions. Objective: This study was conducted to evaluate the effects of the provision of a protein-rich supplement on productive performance, and metabolic profile on grazing suckling female beef calves in tropical conditions during 150 d of experimentation.Methods: Fifty-six Nellore suckling female calves, and their respective dams were distributed in a completely randomised design and made to undergo two treatments as follows: UNS (without supplementation), and SUP (supplementation with 5 g/kg body weight [BW] of a protein supplement). Throughout the experiment, animal performance and metabolic profile were evaluated. Also, ureagenesis and gluconeogenesis were assessed for gene expression.Results: SUP female calves showed a higher voluntary intake (p≤0.03) of the diet components evaluated, digestibility of organic matter (p≤0.02) and microbial nitrogen production (MICN; p≤0.02) compared to UNS female calves. In its turn, serum urea nitrogen (p≤0.01) and insulin-like growth factor-1 (p≤0.03) levels and ureagenesis (p≤0.04) increased in SUP female calves compared to UNS female calves. Blood glucose and triglyceride levels were not affected by supplementation. The average daily gain (ADG) from SUP female calves was higher (p≤0.02) compared with UNS female calves. However, supplementation did not affect the body measures of the animals.Conclusion: In summary, provision of a protein-rich supplement improves the intake and nutrients digestibility, ADG and final BW and increases metabolic indicators of the protein status in grazing suckling female beef calves in tropical conditions.

Development of a Microbial Consortium for Dairy Wastewater Treatment

C. A. Mazzucotelli,I. Durruty,C. E. Kotlar,M. R. Moreira,A. G. Ponce,S. I. Roura 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.2

The wastewater from the dairy industries usuallycontains high concentrations of contaminants and, since thevolume generated is also high, the total contaminant load isvery significant. Among the available options for treatment,biological degradation looks like the most promising one. Furthermore, the supplementation of the native microbialpopulations with external microorganisms with high specificdegradation rates (bio-augmentation) has demonstrated toimprove the performance of treatment. The main objectiveof this research was to select a combination of bacteria toimprove the aerobic treatment of dairy processingwastewater. For this purpose, eleven fat/protein-degradingmicroorganisms belonging to the genera Bacillus, Serratia,Lactococcus, Enterococcus, Stenotrophomonas, Klebsiellaand Escherichia, were evaluated as potential degradingbacteria using a Plackett-Burman design. Assays werecarried out to select the strains that most significantlyinfluenced the degradation of wastewater and biomassyield, in terms of COD removal. A simulated dairyindustry effluent was used as culture medium. Four strainswere selected as potential members of the microbialconsortium: Lactococcus garvieae, Bacillus thuringiensis,Escherichia coli and Stenotrophomonas sp. The optimaloperation temperature and pH range of the selectedconsortium were 32°C and 6 ~ 8, respectively. Thedegradation percentages reached with the selected consortiumwere 80.67 and 83.44% at 24 and 48 h, respectively. Theselected consortium significantly improved the degradationof the dairy wastewater, and the degradation degreeachieved by this consortium was higher than by using thestrains individually.

Reverse Shape Selectivityin the Liquid-PhaseAdsorption of Xylene Isomers in Zirconium Terephthalate MOF UiO-66

Moreira, MarianaA.,Santos, Joã,o C.,Ferreira, Alexandre F. P.,Loureiro, José,M.,Ragon, Florence,Horcajada, Patricia,Shim, Kyu-E.,Hwang, Young-K.,Lee, U.-Hwang,Chang, Jong-S.,Serre, Christi American ChemicalSociety 2012 Langmuir Vol.28 No.13

<P>Powder, agglomerates, and tablets of the microporous zirconium(IV)terephthalate metal–organic framework UiO-66 were evaluatedfor the selective adsorption and separation of xylene isomers in theliquid phase using <I>n</I>-heptane as the eluent. Pulseexperiments, performed at 313 K in the presence of <I>n</I>-heptane, revealed the <I>o</I>-xylene preference of thismaterial, which was further confirmed by binary and multicomponentbreakthrough experiments in the presence of <I>m</I>- and <I>p</I>-xylene, resulting in selectivities at 313 K of 1.8 and2.4 with regards to <I>m</I>-xylene and <I>p</I>-xylene, respectively. Additionally, because <I>p</I>-xyleneis the less retained isomer, UiO-66 presents a selectivity patternthat is reverse of that of the xylenes' molecular dimension with respectto shape selectivity. The shaping of the material as tablets did notsignificantly change its selectivity toward the <I>o</I>-xylene isomer or toward <I>p</I>-xylene, which was theless retained isomer, despite a loss in capacity. Finally, the selectivitybehavior of UiO-66 in the liquid <I>n</I>-heptane phasemakes it a suitable material for <I>o</I>-xylene separationin the extract (heavy product) or <I>p</I>-xylene separationin the raffinate (light product) by simulated moving bed technology.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/langd5/2012/langd5.2012.28.issue-13/la3004118/production/images/medium/la-2012-004118_0011.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/la3004118'>ACS Electronic Supporting Info</A></P>

Anti-Inflammatory and Cicatrizing Activities of a Carbohydrate Fraction Isolated from Sugary Kefir

M.E.C. Moreira,M.H. Dos Santos,G.P.P. Zolini,A.T.B. Wouters,J.C.T. Carvalho,J.M. Schneedorf 한국식품영양과학회 2008 Journal of medicinal food Vol.11 No.2

Kefir is an association of microrganisms generally grown in milk, with known probiotic activities identifiedfrom its soured suspensions. Aqueous media are also able to grow kefir, but little is known about the probiotic properties ofits fermented products. This work aimed to evaluate some probiotic properties of a carbohydrate fraction isolated from sug-ary kefir (sugary kefir carbohydrate [SKC]). Anti-inflammatory activity of the isolated fraction of carbohydrate was testedboth in vitro(cellular respirometry and macrophage culture) and in vivo (50% effective dose, rat paw edema, vascular per-meability, and cicatrizing test). The results indicated no significant difference for oxygen uptake or macrophage culture be-tween control and test groups. Rat paw edema, however, showed a significant inhibitory activity by 30. 4% and 54 . 8%(P. .001) for carrageenan and dextran, respectively. In the cicatrizing test, animals treated with SKC cream also presentedless trauma after treatments as compared to the negative control group (P. .05). The overall data suggested the SKC as anatural product that could be used as a constituent of an anti-inflammatory compound.

Health Equity Research Outcomes and Improvement Consortium Prostate Cancer Health Precision Africa1K: Closing the Health Equity Gap Through Rural Community Inclusion

Vanessa M. Hayes,Sean M. Patrick,Joyce Shirinde,Weerachai Jaratlerdsiri,Mukudeni Nenzhelele,Mulalo B. Radzuma,Kazzem Gheybi,Winstar Mokua,Micah O. Oyaro,Daniel M. Moreira,Ikenna C. Madueke,HEROIC PCaP 대한비뇨기종양학회 2024 Journal of Urologic Oncology Vol.22 No.2

Prostate cancer shows significant racial disparity, with men of African ancestry disproportionately impacted. While prostate cancer health disparity studies focus on elucidating the contributing socioeconomic, lifestyle, environmental, biological and underlying genetic factors, genome sequencing is helping to reduce the burden through disease stratification and treatment. Sub-Saharan Africa has, till now, been excluded from these benefits. The new Prostate Cancer Precision Health Africa1K Health Equity Research Outcomes and Improvement Consortium has been tasked with addressing this gap. Initiating efforts in Southern Africa, with the highest globally recorded regional mortality rates, in this review we discuss our earliest observations, with the objective to share knowledge, encourage further inclusivity across Sub-Saharan Africa, while considering challenges and benefits. Most notably, and in contrast to regions of current scientific efforts, African nations not only represent extreme disparities in rural-urban transition, but our early data also suggests that this transition has direct impact on both genetic and nongenetic health determinants, with further translation into tumour genome disparities. Ultimately, we propose from this first-of-its-kind resource, that rural communities provide an unmet opportunity to control for cultural practices, regional movement, genetic ancestry, and environmental exposures to enhance African inclusion in prostate cancer health disparity studies.

Antidiarrheal Activity of Campomanesia xanthocarpa Fruit

Tatiana M. Souza-Moreira,Luiz Estêvão Salvagnini,Emerson Santos,Viviana Y.A. Silva,Raquel R.D. Moreira,Hérida R.N. Salgado,Rosemeire C.L.R. Pietro 한국식품영양과학회 2011 Journal of medicinal food Vol.14 No.5

The growing list of drug-resistant microorganisms and the persistence of deaths due to diarrhea are compelling reasons to study plants in search of new therapeutic agents. The chemical constitution and popular use of the edible fruits of Campomanesia xanthocarpa O. Berg motivated this study to assess the antimicrobial and antidiarrheal properties of the fruits. An extract in 70% ethanol was prepared, and its antimicrobial activity was tested against several strains of bacteria by the agar diffusion and microdilution methods. Antidiarrheal activity was analyzed by testing intestinal motility in an animal model. Preliminary phytochemical study indicated the presence of flavonoids, saponins, and tannins in the hydroalcoholic extract. Antimicrobial activity was significant, but the minimum inhibitory concentration proved to be higher than the maximum extract concentration tested. The extract did not show significant activity for intestinal motility. Although this fruit extract did not show great results as an antimicrobial or antidiarrheal agent, the study contributes to the search for new plant agents and could be referred to as a research protocol by investigators in this area.

High-performance circular sawing of AISI 1045 steel with cermet and tungsten carbide inserts

A. M. Abrão,M. C. Moreira,P. E. Faria,J. C. Campos Rubio 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.10

This work investigated the influence of cutting speed and feed rate on cutting forces, surface roughness, and slot width circular sawingof AISI 1045 steel. The effects of tool material (cermet and tungsten carbide) and geometry (chip breaker flute and pre-cutting/postcuttingteeth) were also investigated. Thrust and radial forces generally tended to decrease as the cutting speed increased and tended toincrease with the feed rate. The lowest values of thrust and radial forces were obtained using a tungsten carbide saw ground with precuttingand post-cutting teeth. With regard to the quality of the machined wall, the lowest surface roughness was obtained by applying thehighest cutting speed and lowest feed rate and employing a cermet brazed saw. Under this condition, roughness values comparable toface turning and parting off operations were obtained. The cermet brazed saw was responsible for producing the narrowest slot widths.

Phytochemical Characterization, Antimicrobial Activity, and Antioxidant Potential of Equisetum hyemale L. (Equisetaceae) Extracts

Geisiany M. de Queiroz,Fla´vio A.S. Politi,Edvaˆnio R. Rodrigues,Tatiana M. Souza-Moreira,Raquel R.D. Moreira,Ca´ssia R.P. Cardoso,Lourdes C. Santos,Rosemeire C.L.R. Pietro 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.7

Equisetum hyemale species is considered a medicinal plant used in the form of infusions to combat infectious or inflammation diseases and also diuretic effects, presenting several compounds related to these actions. In previous studies different species of Equisetum showed several phenolic compounds. The objective of this study was, for the first time, based on phytochemistry analysis to evaluate the antioxidant and antimicrobial activity. The 70% ethanolic and methanolic extracts of E. hyemale were characterized by spectrophotometric and high-performance liquid chromatography with pulsed amperometric detector analyses, as well as its antioxidant potential based on the scavenger activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH). In addition was verified the antimicrobial activity by broth microdilution technique against bacteria and fungi. The extracts showed phytochemical similarity, which demonstrated the presence of phenolic compounds, the scavenging activity for free radicals was about 30% and was observed better antifungal activity against dermatophyte fungi, with minimum inhibitory concentration and minimum fungicidal concentration of 0.62 mg/mL to Trichophyton rubrum and Microsporum canis. The extracts exhibits great potential to therapeutic applications or product development, since both possess antifungal activity and antioxidant action associated with little difference in their phytochemical composition.

Forced swimming stress increases natatory activity of lead-exposed mice

Araujo Ulisses C.,Krahe Thomas E.,Ribeiro-Carvalho Anderson,Gomes Regina A. A.,Lotufo Bruna M.,Moreira Maria de Fátima R.,de Abreu-Villaça Yael,Manhães Alex C.,Filgueiras Cláudio C. 한국독성학회 2021 Toxicological Research Vol.37 No.1

Recent evidence points to the relationship between lead toxicity and the function of the hypothalamic-pituitary-adrenal axis, which suggests that lead exposure could influence how an individual cope with stress. Here we test this hypothesis by investigating the behavioral effects of lead exposure in mice during the forced swimming test (FST), a parading in which animals are exposed to a stressful situation and environment. Swiss mice received either 180 ppm or 540 ppm of lead acetate (Pb) in their ad-lib water supply for 60–90 days, starting at postnatal day 30. Control (Ctrl) mice drank tap water. At the end of the exposure period, mice were submitted to a 5-min session of FST or to an open-field session of the same duration. Data from naïve animals showed that corticosterone levels were higher for animals tested in the FST compared to animals tested in the open-field. Blood-lead levels (BLL) in Pb-exposed mice ranged from 14.3 to 106.9 μg/dL. No differences were observed in spontaneous locomotion between Ctrl and Pb-exposed groups in the open-field. However, in the FST, Pb-treated mice displayed higher swimming activity than Ctrl ones and this effect was observed even for animals with BLL higher than 20 μg/ dL. Furthermore, significant differences in brain glutathione levels, used as an indicator of led toxicity, were only observed for BLL higher than 40 μg/dL. Overall, these findings suggest that swimming activity in the FST is a good indicator of lead toxicity and confirm our prediction that lead toxicity influences behavioral responses associated to stress.

Cinnamon: an aromatic condiment applicable to chronic kidney disease

Moreira Laís de Souza Gouveia,Brum Isabela de Souza da Costa,de Vargas Reis Drielly C. M.,Trugilho Liana,Chermut Tuany R.,Esgalhado Marta,Cardozo Ludmila F. M. F.,Stenvinkel Peter,Shiels Paul G.,Mafra 대한신장학회 2023 Kidney Research and Clinical Practice Vol.42 No.1

Cinnamon, a member of the Lauraceae family, has been widely used as a spice and traditional herbal medicine for centuries and hasshown beneficial effects in cardiovascular disease, obesity, and diabetes. However, its effectiveness as a therapeutic intervention forchronic kidney disease (CKD) remains unproven. The bioactive compounds within cinnamon, such as cinnamaldehyde, cinnamicacid, and cinnamate, can mitigate oxidative stress, inflammation, hyperglycemia, gut dysbiosis, and dyslipidemia, which are commoncomplications in patients with CKD. In this narrative review, we assess the mechanisms by which cinnamon may alleviate complicationsobserved in CKD and the possible role of this spice as an additional nutritional strategy for this patient group.