TRIM24-Mediated Acetylation of STAT6 Suppresses Th2-Induced Allergic Rhinitis

Yue Liyan,Jia Qiaojing,Dong Jinhui,Wang Jianxing,Ren Xiumin,Xu Ou 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.5

Purpose: Allergic rhinitis (AR) is a T helper type 2 (Th2)-mediated inflammatory disease. The E3 ligase tripartite motif-containing 24 (TRIM24) regulates the recruitment of acetyltransferase CREB-binding protein (CBP) to signal transducer and activator of transcription 6 (STAT6). CBP mediates the acetylation of STAT6 and decreases its activity. To date, the precise role of TRIM24 in AR has not been fully interpreted. Herein, our study aimed to explore the functions of TRIM24 in AR. Methods: The expression of TRIM24 in peripheral blood mononuclear cells (PBMCs) and CD4+ T cells from patients with AR was measured. TRIM24-conditional knockout mice with TRIM24 deficiency in CD4+ T cells were generated. Wide-type (WT) AR mice and TRIM24-conditional knockout AR mice were established. Then, AR symptoms and interleukin (IL)-4 levels were compared. Further, the proliferation, activation and polarization of CD4+ T cells from WT mice and TRIM24 knockout mice after stimulation were determined. The effects of TRIM24 deficiency on STAT6 activities were also evaluated. Results: Downregulated TRIM24 expression was detected in PBMCs and CD4+ T cells from patients with AR. TRIM24 conditional knockout mice had more sever AR symptoms with elevated IL-4 production. TRIM24-knockout CD4+ T cells had similar proliferation and activation when compared to WT CD4+ T cells, while they had enhanced Th2 polarization. TRIM24-knockout CD4+ T cells had decreased acetylation of STAT6 and enhanced STAT6 activities after IL-4 stimulation. The regulation of STAT6 activities by TRIM24 depended on TRIM24 N terminal RIGN domain and Lys383 acetylation site of STAT6. Conclusions: TRIM24 suppresses Th2-mediated AR by regulating the acetylation of STAT6.

Molecularly imprinted electrospun nanofibre membrane assisted stir bar sorptive extraction for trace analysis of sulfonamides from animal feeds

Yahan Cui,Liyan Jiang,Huiyu Li,Di Meng,Yanhua Chen,Lan Ding,Yang Xu 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.96 No.-

The widespread addition of sulfa antibiotics in feeds has long been an environment safety issue. Therefore, it is quite meaningful to constantly propose better solutions for determining the sulfaantibiotics in the environment. Herein, molecularly imprinted electrospun nanofibre membrane (MIM)assisted stir bar sorptive extraction method for the determination sulfonamides (SAs) in feed wasdeveloped. In this study, molecularly imprinted polymers (MIPs) werefirstly prepared via emulsionpolymerization using sulfamonomethoxine as template. Then, the morphology-controllable MIM wasobtained with MIPs-doping electro-spinning solution using electro-spinning technique. Next, MIM arecoated on the magnetic stir bar by heat-sealing. Finally, MIM coated stir bar was used for selectiveenrichment of SAs. The obtained MIM exhibited excellent class selectivity towards SAs (the selectivityfactor β is 2.3–2.7). The MIM coated stir bar can be directly applied to extract SAs from feed samples. Under the optimum extraction conditions, the detection limit of the method could reach 1.5 3.4 ng/g. And satisfactory recoveries ranging from 80 6% to 89 7% were achieved under all three spiked levelsfor determination of four SAs infive different feed samples. The present work not only offers insights todevelop magnetic stir bar using imprinted membrane, but also provides a new method for SAs extraction.

Breast Cancer Metastatic to Gluteus Maximus: A Case Report

Simon Roh,Liyan Xu 한국유방암학회 2023 Journal of Breast Disease Vol.11 No.1

Breast cancer rarely metastasizes to skeletal muscle. Moreover, skeletal muscle is a rare site of metastasis for any type of cancer. In this study, we report an unexpected finding of an enlarged mass in the gluteus maximus of an asymptomatic patient with a distant history of breast cancer. The mass was observed during computed tomography evaluation in preparation for a non-oncologic procedure. Biopsy and pathological investigation confirmed breast cancer metastasis. An increased awareness of rare metastatic sites is important so that such lesions are critically evaluated during early imaging examination.

Regional Economic Evaluation Based on a Novel Fuzzy Clustering Method in Big data

Liu Zhaohui,Li Liyan,Xu Wenjing 보안공학연구지원센터 2014 International Journal of Database Theory and Appli Vol.7 No.3

A study of P release from Fe-P and Ca-P via the organic acids secreted by Aspergillus niger

Tian Da,Wang Liyan,Hu Jun,Zhang Liangliang,Zhou Ningning,Xia Jingjing,Xu Meiyue,Yusef Kianpoor Kalkhajeh,Wang Shimei,Li Zhen,Gao Hongjian 한국미생물학회 2021 The journal of microbiology Vol.59 No.9

Phosphate solubilizing fungi (PSF) have been widely applied to dissolve insoluble phosphates (IPs). However, the PSF usually demonstrates a different phosphate solubilizing capacity for various IPs. This study explored the mechanisms of Aspergillus niger for the dissolution of ferric phosphate (FePO4, Fe-P), and tricalcium phosphate (Ca3[PO4]2, Ca-P) regarding the tricarboxylic acid (TCA) cycle. Aspergillus niger has higher phosphorus (P) content released from Ca-P, reached the maximum value of 861 mg/L after seven days of incubation, compared with the 169 mg/L from Fe-P. Oxalic acid promoted the release of P from Ca-P through the formation of calcium oxalate. The presence of Fe-P can stimulate A. niger to secrete large amounts of citric acid, confirmed by the enhancement of citrate synthase (CS) activity. However, citric acid only promotes 0.5% of P released from Fe-P. Meanwhile, although oxalic acid still dominates the release of P from Fe-P, its abundance was significantly declined. In contrast, oxalic acid also shows a higher P release ratio in Ca-P than citric acid, i.e., 36% vs. 22%. This study points to the future usage of A. niger to dissolve IPs in soil required to enhance oxalic acid secretion.

Multicenter, randomized study of genetically modified recombinant human interleukin-11 to prevent chemotherapy-induced thrombocytopenia in cancer patients receiving chemotherapy

Wu, Shikai,Zhang, Yang,Xu, Liyan,Dai, Yun,Teng, Yuee,Ma, Shanshan,Ho, Seong-Hyun,Kim, Jong-Mook,Yu, Seung Shin,Kim, Sunyoung,Song, Santai Springer-Verlag 2012 Supportive care in cancer Vol.20 No.8

Foeniculum vulgare essential oil nanoemulsion inhibits Fusarium oxysporum causing Panax notoginseng root-rot disease

Hongyan Nie,Hongxin Liao,Jinrui Wen,Cuiqiong Ling,Liyan Zhang,Furong Xu,Xian Dong The Korean Society of Ginseng 2024 Journal of Ginseng Research Vol.48 No.2

Background: Fusarium oxysporum (F. oxysporum) is the primary pathogenic fungus that causes Panax notoginseng (P. notoginseng) root rot disease. To control the disease, safe and efficient antifungal pesticides must currently be developed. Methods: In this study, we prepared and characterized a nanoemulsion of Foeniculum vulgare essential oil (Ne-FvEO) using ultrasonic technology and evaluated its stability. Traditional Foeniculum vulgare essential oil (T-FvEO) was prepared simultaneously with 1/1000 Tween-80 and 20/1000 dimethyl sulfoxide (DMSO). The effects and inhibitory mechanism of Ne-FvEO and T-FvEO in F. oxysporum were investigated through combined transcriptome and metabolome analyses. Results: Results showed that the minimum inhibitory concentration (MIC) of Ne-FvEO decreased from 3.65 mg/mL to 0.35 mg/mL, and its bioavailability increased by 10-fold. The results of gas chromatography/mass spectrometry (GC/MS) showed that T-FvEO did not contain a high content of estragole compared to Foeniculum vulgare essential oil (FvEO) and Ne-FvEO. Combined metabolome and transcriptome analysis showed that both emulsions inhibited the growth and development of F. oxysporum through the synthesis of the cell wall and cell membrane, energy metabolism, and genetic information of F. oxysporum mycelium. Ne-FvEO also inhibited the expression of 2-oxoglutarate dehydrogenase and isocitrate dehydrogenase and reduced the content of 2-oxoglutarate, which inhibited the germination of spores. Conclusion: Our findings suggest that Ne-FvEO effectively inhibited the growth of F. oxysporum in P. notoginseng in vivo. The findings contribute to our comprehension of the antifungal mechanism of essential oils (EOs) and lay the groundwork for the creation of plant-derived antifungal medicines.

Inhibition of Dll4/Notch1 pathway promotes angiogenesis of Masquelet’s induced membrane in rats

Qian Tang,Haimin Jin,Minji Tong,Gang Zheng,Zhongjie Xie,Shangkun Tang,Jialei Jin,Ping Shang,Huazi Xu,Liyan Shen,Yu Zhang,Haixiao Liu 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

The Masquelet’s induced membrane technique for repairing bone defects has been demonstrated to be a promising treatment strategy. Previous studies have shown that the vessel density of induced membrane is decreased in the late stage of membrane formation, which consequently disrupts the bone healing process. However, relatively little is known about certain mechanisms of vessel degeneration in the induced membrane tissue and whether promotion of angiogenesis in induced membranes can improve bone regeneration. Here, we showed that the Delta-like ligand 4/ Notch homolog 1 (Dll4/Notch1) pathway was relatively activated in the late stage of induced membrane, especially at the subcutaneous site. Then, DAPT, a classical γ-secretase inhibitor, was applied to specifically inhibit Notch1 activation, followed by up-regulation of vascular endothelial growth factor receptor 2 (VEGFR2) and CD31 expression. DAPTmodified induced membranes were further confirmed to contribute to bone regeneration after autogenous bone grafting. Finally, in vitro experiments revealed that knocking down Notch1 contributed to the functional improvement of endothelial progenitor cells (EPCs) and that DAPT-treated induced membrane tissue was more favorable for angiogenesis of EPCs compared with the vehicle group. In conclusion, the present findings demonstrate that Dll4/ Notch1 signaling is negatively associated with the vessel density of induced membrane. Pharmacological inhibition of Notch1 attenuated the vessel degeneration of induced membrane both in vitro and in vivo, which consequently improved bone formation at the bone defect site and graft resorption at the subcutaneous site.

CT Synthesis from MRI Using Generative Adversarial Network with Frequency-Aware Discriminator

Li Yan,Xu Sisi,Qi Zhenyu 대한전기학회 2024 Journal of Electrical Engineering & Technology Vol.19 No.1

The pursuit of generating computed tomography (CT) from magnetic resonance imaging (MRI) remains a key area of research with the goal of advancing modern radiation therapy. There has been an increased emphasis on leveraging deep learning methodologies, particularly the generative adversarial network (GAN), to convert MRI into CT. The efectiveness of GAN training hinges on the capacity of its discriminator model to identify and rectify faws in the synthetic CT, providing valuable feedback to the generator model. Acknowledging the multi-scale complexity of human anatomy, this study introduces an innovative discriminator model, designed to assess the synthetic performance across varying scales and frequencies of tissues and organs. We evaluated the signifcance of this frequency-aware discriminator by contrasting it with two commonly used discriminator models: the convolutional neural network discriminator and PatchGAN. We conducted our testing within three existing GAN frameworks on a dataset of 78 nasopharyngeal carcinoma patients. The experimental outcomes revealed that our model managed to decrease the mean absolute error between synthetic and actual CT by an average of 0.18–1.55 Hounsfeld Units within these frameworks. Additionally, it enhanced the visual quality of synthetic CT, ofering superior local structures and patterns. These fndings suggest that our newly developed discriminator can ofer comprehensive guidance to the generator, thereby enhancing CT synthetic performance.

Synthesis of full concentration gradient cathode studied by high energy X-ray diffraction

Li, Yan,Xu, Rui,Ren, Yang,Lu, Jun,Wu, Huiming,Wang, Lifen,Miller, Dean J.,Sun, Yang-Kook,Amine, Khalil,Chen, Zonghai Elsevier 2016 Nano energy Vol.19 No.-

<P><B>Abstract</B></P> <P>Nickel-rich metal oxides have been widely pursued as promising cathode materials for high energy-density lithium-ion batteries. Nickel-rich lithium transition metal oxides can deliver a high specific capacity during cycling, but can react with non-aqueous electrolytes. In this work, we have employed a full concentration gradient (FCG) design to provide a nickel-rich core to deliver high capacity and a manganese-rich outer layer to provide enhanced stability and cycle life. <I>In situ</I> high-energy X-ray diffraction was utilized to study the structural evolution of oxides during the solid-state synthesis of FCG lithium transition metal oxide with a nominal composition of LiNi<SUB>0.6</SUB>Mn<SUB>0.2</SUB>Co<SUB>0.2</SUB>O<SUB>2</SUB>. We found that both the pre-heating step and the sintering temperature were critical in controlling phase separation of the transition metal oxides and minimizing the content of Li<SUB>2</SUB>CO<SUB>3</SUB> and NiO, both of which deteriorate the electrochemical performance of the final material. The insights revealed in this work can also be utilized for the design of other nickel-rich high energy-density cathode materials.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Solid-state synthesis of FCG cathode is investigated by <I>in situ</I> XRD. </LI> <LI> Covariance analysis and Rietveld refinement are used to analyze the HEXRD data. </LI> <LI> Synthetic optimization of FCG cathode with excellent electrochemical performance. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Benefit from the covariance analysis and Rietveld refinement of <I>in situ</I> HEXRD data during the solid state synthesis, we can optimized the solid state synthesis conditions in a short time. And the full concentration gradient cathode composites (nickel-rich core and manganese-rich outer layer) with excellent electrochemical performance are obtained.</P> <P>[DISPLAY OMISSION]</P>