Hypoxia Mediates Runt-Related Transcription Factor 2 Expression via Induction of Vascular Endothelial Growth Factor in Periodontal Ligament Stem Cells

Manjing Deng,Qian Xu,Zhihua Liu1,Ling Guo,Rui Liu,Rulei Li,Xiang Chu,Jiajia Yang,Jia Luo,Faming Chen 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.11

Periodontitis is characterized by the loss of periodontal tissues, especially alveolar bone. Common therapies cannot satisfactorily recover lost alveolar bone. Periodontal ligament stem cells (PDLSCs) possess the capacity of self-renewal and multilineage differentiation and are likely to recover lost alveolar bone. In addition, periodontitis is accompanied by hypoxia, and hypoxia-inducible factor-1α (HIF-1α) is a master transcription factor in the response to hypoxia. Thus, we aimed to ascertain how hypoxia affects runt-related transcription factor 2 (RUNX2), a key osteogenic marker, in the osteogenesis of PDLSCs. In this study, we found that hypoxia enhanced the protein expression of HIF-1α, vascular endothelial growth factor (VEGF), and RUNX2 ex vivo and in situ. VEGF is a target gene of HIF-1α, and the increased expression of VEGF and RUNX2 proteins was enhanced by cobalt chloride (CoCl2, 100 μmol/L), an agonist of HIF-1α, and suppressed by 3-(5′-hydroxymethyl-2′-furyl)-1-benzyl indazole (YC-1, 10 μmol/L), an antagonist of HIF-1α. In addition, VEGF could regulate the expression of RUNX2, as RUNX2 expression was enhanced by human VEGF (hVEGF165) and suppressed by VEGF siRNA. In addition, knocking down VEGF could decrease the expression of osteogenesis-related genes, i.e., RUNX2, alkaline phosphatase (ALP), and type I collagen (COL1), and hypoxia could enhance the expression of ALP, COL1, and osteocalcin (OCN) in the early stage of osteogenesis of PDLSCs. Taken together, our results showed that hypoxia could mediate the expression of RUNX2 in PDLSCs via HIF-1α-induced VEGF and play a positive role in the early stage of osteogenesis of PDLSCs

Mechanical evaluation of polymer microneedles for transdermal drug delivery: In vitro and in vivo

Rui Xuan Liu,Yu Ting He,Ling Liang,Liu Fu Hu,Yue Liu,Rui-xing Yu,Bo Zhi Chen,Yong Cui,Xin Dong Guo 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

In this study, we reported two types of PMNs based on polylactic acid (PLA) and polyvinyl alcohol (PVA),respectively. Parafilm M film, porcine skin, and rats’ models were operated to evaluate the mechanicalproperties in vitro and in vivo to find optimal parameters for efficient insertion. Insertion depth was measuredusing Digital Force Gauge by changing insertion force and speed, respectively. Results showed thatincreasing the insertion force and speed used for PMNs application led to a significant increase in thedepth of insertion. A force of 18 N under a speed of 330 mm/min was the optimal condition for insertingPMNs array into ParafilmM film and porcine skin. In addition, PLA-MNs exhibited higher robustness andenhanced homogeneity in insertion depth compared with PVA-MNs, but PVA-MNs were able to reachmuch deeper insertion depth. Moreover, Sprague Dawley (SD) rat experiments confirmed the effectivenessof optimal insertion parameters for transdermal drug delivery. This study illustrated not only thedevelopment of novel PMNs but also the mechanical evaluation for the design of PMNs.

In Vitro Evaluation and Monitoring of the Expression Level and Localization of Aldose Reductase Using Functionalized Quantum Dots and EGFP

Xiaomin Liu,Chengbin Yang,Jing Liu,Jianwei Liu,Rui Hu,Hongwei Lian,Guimiao Lin,Liwei Liu,Ken-Tye Yong,Ling Ye 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

The optimization of aldose reductase (AR) expression levels and tracking of the AR expression sites within the cell is an essential step in developing a platform for the effective production of aldose reductase inhibitors (ARIs). In this study, we have demonstrated the use of both immunocytochemistry and quantum dots-based immunofluorescence techniques for observing and detecting the expression level and localization of AR in the cytoplasm and cell membrane of a eukaryotic cell model with high levels of AR protein expression. Our results show that high expression levels of human AR can be achieved using the eukaryotic cell model that we have developed. The overexpressed AR can be used for translational studies of hAR and the screening of ARIs. More importantly, the use of the established quantum dots-based immunofluorescence technique in the intracellular labeling of AR allows the determination of the expression and distribution of the AR gene. Overall, the use of the interdisciplinary approach of both genetic engineering and quantum dot-based immunofluorescence allows not only the effective production of a desired protein, but also the determination of the cellular localization of such an expressed protein.

Synthesis and characterization of core–shell associative polymer that prepared by oilfield formation water for chemical flooding

Rui Liu,Wan-Fen Pu,Dai-Jun Du 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.46 No.-

A novel core–shell polymer (HBAPAM) with functionalized nano-SiO2 as core, multibranched associativepolymeric chains as shell was synthesized through in-situ free radical polymerization strategy. TGA, IR,1H NMR, and SEM proved the notion and method of preparing of HBAPAM was applicable. The core–shellstructure and multibranched morphology enhanced the supramolecular interaction of HBAPAM, whichdisplayed the desirable viscosified efficiency and viscoelasticity, especially, long-term stability in harshtemperature and salinity condition. HBAPAM featured the rheology in multiscale consistently indeionized water and formation water. Coreflooding experiments confirmed that HBAPAM had high costperformance in the variable permeability of oilfields.

아이트래킹(Eye Tracking)을 활용한 재무제표 응시 결과 분석

Rui Yi Zhang,나형종,Ya Lei Liu,Jun Song,Yue Yan Liu 세명대학교 인문사회과학연구소 2024 人文 社會科學硏究 Vol.32 No.1

· Research topics: This paper presents research results on people's understanding of financial statements and information delivery power of financial statements using eye tracking technology. · Research background: In the field of social science, research that introduces eye-tracking experiments is also being conducted, but in the field of accounting, eye-tracking research through eye-tracking is insufficient. In this paper, research is conducted by introducing eye-tracking techniques to determine whether the disclosure form of financial statements in accordance with the current international accounting standards adopted in Korea has high information transmission power. · Differences from prior research: This study is a fusion and convergence study that innovatively used eye tracking techniques to study topics in the field of accounting. Through this, meaningful information is provided to understand the understanding and information delivery power of the current major financial statements adopted in Korea. · Research method: This paper presented the results of empirical analysis by conducting an experiment to gaze at financial statements using eye tracking technology. In addition, this study conducted a survey on financial statement information delivery power to verify the information delivery power of financial statements in accordance with the current international accounting standards adopted in Korea. · Research Results: As a result of i-Tracking research, it was found that in the statement of absence, most eyes were on current assets, non-current assets, current liabilities, non-current liabilities, and capital. In the statement of comprehensive income, it was found that most eyes were on the order of sales, cost of sales, net income and other comprehensive income, operating profit, and total comprehensive income. And it was found that relatively few eyes were on gross profit, sales and management expenses, and non-operating expenses. In the table of capital fluctuations, it was found that many people applied in the order of capital, excess issuance of stocks, and total capital. And it was found that relatively few people stared at retained earnings and other capital items. Lastly, in the cash flow table, it was found that people's eyes were on the order of operating cash flow, net increase and decrease of cash and cashable assets, investment cash flow, and financial activity cash flow. · Contributions and expected effects: First, the research methodology used in this study is a unique research methodology that is rarely used in the field of accounting, and is expected to provide good guidelines for research in the field of accounting by introducing various methods. Second, the research results of this paper provide meaningful information to understand the comprehension and information delivery power of the current major financial statements adopted in Korea, and further improvements are made to the form of financial statement disclosure to improve the usefulness of financial statement information. · 연구 주제: 본 논문은 아이트래킹 기술을 활용하여 사람들의 재무제표의 이해도 및 재무제표의 정보전달력에 관한 연구결과를 제시한다. · 연구 배경: 사회과학 분야에서도 아이트래킹 실험을 도입한 연구가 진행되고 있지만, 회계학 분야에서는 아이트래킹을 통한 시선 추적 연구가 미흡한 실정이다. 본 논문에서는 현행 한국 채택 국제회계기준에 의한 재무제표의 공시형태가 과연 높은 정보 전달력이 있는지를 규명하기 위해 아이트래킹 기법을 도입하여 연구를 수행한다. · 선행연구와의 차이점: 본 연구는 회계학 분야의 주제를 연구하는데 있어서 혁신적으로 아이트래킹 기법을 사용하여 실험을 수행한 융·복합 연구이다. 이를 통하여 한국 채택 현행 주요 재무제표의 이해가능성 및 정보전달력을 파악하는데 의미 있는 정보를 제공한다. · 연구방법: 본 논문은 아이트래킹 기술을 활용하여 재무제표를 응시하는 실험을 수행하여 실증분석 결과를 제시하였다. 또한 본 연구에서는 현행 한국 채택 국제회계기준에 의한 재무제표의 정보전달력을 검증하기 위하여 재무제표 정보전달력에 관한 설문조사를 수행하였다. · 연구결과: 아이트래킹 연구결과, 제무상태표에서는 유동자산, 비유동자산, 유동부채, 비유동부채, 자본 순으로 시선이 많이 가는 것으로 파악되었다. 포괄손익계산서에서는 매출액, 매출원가, 당기순이익·기타포괄손익, 영업이익, 총포괄손익 순으로 시선이 많이 가는 것으로 나타났다. 그리고 상대적으로 매출총이익, 판매비와 관리비, 영업외비용에는 시선이 적게 가는 것으로 나타났다. 자본변동표에서는 자본금, 주식발행초과금, 자본합계 순으로 사람들이 많이 응시하는 것으로 파악되었다. 그리고 상대적으로 이익잉여금과 기타자본항목은 사람들이 적게 응시하는 것으로 파악되었다. 마지막으로 현금흐름표에서는 영업활동 현금흐름, 현금및현금성자산의 순증감, 투자활동 현금흐름, 재무활동 현금흐름 순으로 사람들의 시선이 많이 가는 것으로 나타났다. · 공헌점 및 기대효과: 첫째, 본 연구에서 사용한 연구방법론은 회계학 분야에서 거의 사용되지 않는 독특한 연구방법론으로써 회계학 분야의 연구가 다양한 방법을 도입하여 발전하는데 있어서 좋은 가이드라인을 제시할 수 있을 것으로 기대된다. 둘째. 본 논문의 연구결과는 한국 채택 현행 주요 재무제표의 이해가능성 및 정보전달력을 파악하는데 의미 있는 정보를 제공하고, 더 나아가 재무제표 정보의 유용성을 향상시키기 위한 재무제표 공시형태에 대해서 개선점을 제시한다.

CL SHAVER FOR LOVERS

Liu Rui 한국콘텐츠학회 2019 ICCC International Digital Design Invitation Exhib Vol.2019 No.12

CL SHAVER FOR LOVERS

Liu Rui 한국콘텐츠학회 2019 한국콘텐츠학회 ICCC 논문집 Vol.2019 No.12

Potent in Vitro Anticancer Activity of Metacycloprodigiosin and Undecylprodigiosin from a Sponge-Derived Actinomycete Saccharopolyspora sp. nov.

Rui Liu,Cheng-Bin Cui,Lin Duan,Qian-Qun Gu,Wei-Ming Zhu 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.12

Bioassay-guided fractionation of CHCl3 extract from the fermentation broth of a sponge Mycale plumose-derived actinomycete Saccharopolyspora sp. nov., led to the isolation of two known prodigiosin analogs - metacycloprodigiosin (1) and undecylprodigiosin (2). These compounds exhibited significant cytotoxic activities against five cancer cell lines: P388, HL60, A-549, BEL- 7402, and SPCA4. This is the first report on the significant cytotoxicity of metacycloprodigiosin (1) against human cancer cell lines.

Two Indolocarbazole Alkaloids with Apoptosis Activity from a Marine-derived Actinomycete Z2039-2

Rui Liu,Tianjiao Zhu,Dehai Li,Jingyan Gu,Wei Xia,Yuchun Fang,HongbingLiu,Weiming Zhu,Qianqun Gu 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.3

Bioassay-guided fractionation of the EtOAc extract from the fermentation broth of a marinederived actinomycete Z2039-2 led to the isolation of two known indolocarbazole alkaloids, K252c (1) and arcyriaflavin A (2). 1 and 2 exhibited moderate cytotoxic activities against the K562 cell line, and induced apoptotic activities at 10 and 100 µM, respectively. This is the first report on the significant apoptosis inducing effect of indolocarbazole alkaloids against K562 cancer cells.

Relative Diagnosability of Discrete-Event Systems and Its Opacity-Based Test Algorithm

Rui Zhao,Fuchun Liu,Zhusong Liu 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.4

The notion of relative diagnosability is proposed for logical automata and the concept of diagnosablerate is introduced to characterize the diagnosability property of a discrete-event system, which takes values in theinterval [0, 1]. The relationship between relative diagnosability and diagnosability introduced by Sampath et al. that relative diagnosability is weaker than diagnosability for discrete-event systems is analysed. Furthermore, anecessary and sufficient condition for relative diagnosability is presented. In particular, an opacity-based algorithmis developed to test the relative diagnosability, which is polynomial in the number of states of the system. Inaddition, the proposed algorithm may be used to deal with the problem of diagnosability.