효모에서 paromomycin 내성에 영향을 미치는 PAR 유전자의 분자유전학적 분석

나윤선,박민철 가톨릭대학교 자연과학연구소 2003 자연과학논문집 Vol.24 No.-

Aminoglycoside계의 항생물질은 Escherichia coli와 yeast에서 전사종결코돈의 인식에 영향을 미치며, aminoglycoside계의 항생물질인 paromomycin이 Saccharomyces cereviside 구조유전자 내의 사슬종결 돌연변이(nonsense mutation)를 억제하는 것에 대한 보고가 있다. Yeast에서 분리된 asu9은 nonsense suppressor인 sup45 efficiency를 감소시키는 antisuppressor로서 작용하고 asu9과 sup45는 모두 paromomycin에 감수성을 보이는 것으로 보고 되어있다. 이러한 asu9의 분석을 위해 translational mutation인 asu9-1에 상보적인 야생형 유전자 ASU9의 cloning이 시도되던 중 asu9-1의 paromomycin 감수성에 대해 상보적 multicopy plasmid인 pJS1이 ASU9 yeast strain이 도입되었을 때도 강한 paromomycin 내성형질을 나타냄이 관찰보고 되었다. 따라서 yeast 내에서 multicopy로 존재할 때 paromomycin 내성형질을 보이는 유전자에 대한 규명이 요구되어져 본 연구에서는 pJS1에 삽입된 유전자에 대한 연구를 시행하여 pJS1에 삽입된 유전자들 중 paromomycin 내성에 관여하는 유전자를 subcloning함으로써 이들 유전자의 분자유전학적인 분석을 시도하였다. pJS1으로부터 제작된 plasmid deletion을 yeast strain JS143-7D에 형질전환시켰을 때 나타난 paromomycin 내성형질을 통해 paromomycin 내성에 관여하는 유전자들(PARs)의 자위를 분석하였으며, copy number에 따른 유전자 발현의 활성을 비교하였다. Aminoglycoside antibiotics in Escherichia coli and yeast can cause ribosomes to read through stop-codons during translation. Aminoglycoside paromomycin can suppress a number of mutations which give rise to stop codons within the structural genes of Saccharomyces cerevisiae. The asu9 isolated as a gene affecting efficiency of nonsense suppressor in Saccharomyces cerevisiae reduces the efficiency of sup45 as a antisuppressor. Both asu9 and sup45 cause sensitivity to paromomycin in yeast. In attempting of the cloning of the ASU9 gene to investigate the molecular nature of asu9, it was reported that pJS1 isolated as a multicopy plasmid complementing the paromomycin sensitivity in asu9 strain contained some genes to cause paromomycin resistance when exist in multicopy number. In order to investigate the genes(PARs) conffering paromomycin resistance when exist in multicopy number, this study was carried out through the molecular genetics analysis of PAR genes. Plasmid deletions of pJS1 were constructed to localize the PAR genes in the inserted DNA of pJS1. Then these plasmid-deletions were transformed into yeast strain, JS143-7D and the paromomycin-resistance phenotype of the resulted transformants was analyzed. Other roles of PAR genes in yeast cells were also investigated.

섬유강화형 포스트를 이용한 치관-치근 파절의 치료: 증례 보고

임화신,라지영,이광희,안소연,김윤희,금기석,이상봉 大韓小兒齒科學會 2012 大韓小兒齒科學會誌 Vol.39 No.1

The crown-root fracture is defined as a fracture of tooth that contains enamel, dentin and cementum with or without pulp exposure. Generally the fracture lines place obliquely from labial surface, between incisal edge of the crown and marginal gingiva, to palatal surface subgingivally. If the fracture line is located supragingivally, the removal of tooth fragment and supragingival restoration can be performed. In subgingival fracture line, the surgical exposure, orthodontic eruption or surgical eruption can be considered. If the fracture line is too deep to restorate, extraction or decoronation can be selected. In children and adolescents, the extraction should be the last option. Another option to select before extraction is the restoration using fiber-reinforced post and the reattachment of tooth fragment. The fiber-rainforced post enhances the retention and the durability of tooth fragment. The reattachment of crown fragment using resin adhesive system is considered minimal invasive treatment biologically. This case reports the treatment of crown-root fracture using the reattachment of crown fragment and the insertion of fiber-reinforced post. 치관-치근 파절은 법랑질, 상아질, 백악질이 모두 포함된 치아의 파절로, 파절선이 대부분 절단연이나 순측의 변연부 치은 에서 구개측 치은열구 하방으로 사선으로 진행된다. 파절선의 위치가 치은 연상이라면 파절편의 제거 및 치은연상 수복을 시 행하고, 치은 연하라면 파절면의 외과적 노출술, 교정적 정출술, 외과적 정출술을 이용한 치아의 수복이 이루어진다. 그 외에 섬유 강화형 포스트를 삽입하여 치관 수복물의 유지력을 높이고, 레진 접착 시스템을 사용하여 치관 파절편을 재부착함으로 써 생물학적으로 최소한으로 침습적인 치료를 하는 방법도 있다. 만약 파절의 정도가 치은 연하로 깊은 경우 발치나 치관절제 술을 이용한 치근의 유지 등을 고려할 수 있다. 본 증례는 치관-치근파절로 내원한 12세 환아로 섬유강화형 포스트를 사용하여 치관 파절편 재부착을 시행하였으며 양호 한 경과를 보여 이를 보고하는 바이다.

영어와 한국어의 활음에 대한 연구

나기연 대한언어학회 1994 언어학 Vol.2 No.-

La, Ki-Yun(1994). A Study on English and Korean Glides. Linguistics, Vol. 2. The purpose of this paper is (i) to suggest that through the universal definition of glides, English glides are divided into onglides and offglides, and their functions and underlying representations are different according to their positions in English syllable structure, (ii) to propose that Korean glides are only onglides, but their underlying and phonetic representation are different in Korean syllable structure, and (iii) to justify the validity of the above second proposal through the discussion of Korean phonological phenomena related to glides. Especially, in the discussion of English and Korean syllable structure, the framework of Levin(1985) is employed for the skeleton tier and the zero feature specification of Hyman(1985) for the underlying representation of glides.

Current Practices in Breast Magnetic Resonance Imaging: a Survey Involving the Korean Society of Breast Imaging

Yun, Bo La,Kim, Sun Mi,Jang, Mijung,Kang, Bong Joo,Cho, Nariya,Kim, Sung Hun,Koo, Hye Ryoung,Chae, Eun Young,Ko, Eun Sook,Han, Boo-Kyung Korean Society of Magnetic Resonance in Medicine 2017 Investigative Magnetic Resonance Imaging Vol.21 No.4

Purpose: To report on the current practices in breast magnetic resonance imaging (MRI) in Korea. Materials and Methods: We invited the 68 members of the Korean Society of Breast Imaging who were working in hospitals with available breast MRI to participate in a survey on how they performed and interpreted breast MRI. We asked one member from each hospital to respond to the survey. A total of 22 surveys from 22 hospitals were analyzed. Results: Out of 22 hospitals, 13 (59.1%) performed at least 300 breast MRI examinations per year, and 5 out of 22 (22.7%) performed > 1200 per year. Out of 31 machines, 14 (45.2%) machines were 1.5-T scanners and 17 (54.8%) were 3.0-T scanners. All hospitals did contrast-enhanced breast MRI. Full-time breast radiologists supervised the performance and interpreted breast MRI in 19 of 22 (86.4%) of hospitals. All hospitals used BI-RADS for MRI interpretation. For computer-aided detection (CAD), 13 (59.1%) hospitals sometimes or always use it and 9 (40.9%) hospitals did not use CAD. Two (9.1%) and twelve (54.5%) hospitals never and rarely interpreted breast MRI without correlating the mammography or ultrasound, respectively. The majority of respondents rarely (13/21, 61.9%) or never (5/21, 23.8%) interpreted breast MRI performed at an outside facility. Of the hospitals performing contrast-enhanced examinations, 15 of 22 (68.2%) did not perform MRI-guided interventional procedures. Conclusion: Breast MRI is extensively performed in Korea. The indication and practical patterns are diverse. The information from this survey would provide the basis for the development of Korean breast MRI practice guidelines.

Does Adding Diffuse Optical Tomography to Sonography Improve Differentiation Between Benign and Malignant Breast Lesions? : Observer Performance Study

La Yun, Bo,Kim, Sun Mi,Jang, Mijung,Ahn, Hye Shin,Lyou, Chae Yeon,Kim, Mi Sun,Kim, Sun Ah,Song, Tai-Kyong,Yoo, Yangmo,Chang, Jin Ho,Kim, Youngmi American Institute of Ultrasound in Medicine 2015 Journal of ultrasound in medicine Vol.34 No.5

<P>Objectives-The purpose of this study was to investigate the added value of diffuse optical tomographic categories combined with conventional sonography for differentiating between benign and malignant breast lesions. Methods-In this retrospective database review, we included 145 breast lesions (116 benign and 29 malignant) from 145 women (mean age, 46 years; range, 16-86 years). Five radiologists independently reviewed sonograms with and without a diffuse optical tomographic category. Each lesion was scored on a scale of 0% to 100% for suspicion of malignancy and rated according to the American College of Radiology Breast Imaging Reporting and Data System classification. Diagnostic performance was analyzed by comparing area under receiver operating characteristic curve values. Reader agreement was assessed by intraclass correlation coefficients. Results-In the multireader multicase receiver operating characteristic analysis, adding a diffuse optical tomographic category to sonography improved the diagnostic accuracy of sonography (mean areas under the curve, 0.923 for sonography alone and 0.969 for sonography with diffuse optical tomography; P = .039). The interobserver correlation was also improved (0.798 for sonography alone and 0.904 for sonography with diffuse optical tomography). The specificity increased for 4 reviewers from a mean of 19.5% to 45.8% (P < .001 for reviewers 1-4; P = .238 for reviewer 5) with no significant change in the sensitivity. When the diffuse optical tomographic category was applied strictly, the specificity increased for all reviewers from a mean of 19.5% to 68.3% (P < .001 for all reviewers) with no significant change in the sensitivity. Conclusions-The addition of diffuse optical tomographic categories to sonography may improve diagnostic performance and markedly decrease false-positive biopsy recommendations.</P>

LGMD2E with a novel nonsense variant in SGCB gene: a case of LGMD2E with a novel variant

Yun Kyung La,Eun Kyoung Oh,Hyun Ji Lyou,홍지만,최영철 대한임상신경생리학회 2020 Annals of Clinical Neurophysiology Vol.22 No.1

Sarcoglycanopathies are a rare group of autosomal recessive limb-girdle muscular dystrophies (LGMDs) caused by genetic variants in α-, β-, γ-, or δ-sarcoglycan that maintain membrane integrity and contribute to molecular signal processing. High-throughput nucleotide sequencing was performed in patients with slowly progressive proximal muscle weakness from early childhood with respiratory involvement, which detected a novel homozygous nonsense variant (c.601C>T;p.Gln201Ter) in SGCB. This report informs about the clinical characteristics of LGMD2E (type-2E LGMD) in Korea and provides genetic confirmation of the disease.

단섬유를 혼입한 미네랄 하이드레이트 단열소재의 물리적 특성

라윤호(La, Yun Ho),박재완(Park, Jae Wan),서성관(Seo, Sung Kwan),추용식(Chu, Yong Sik),송훈(Song, Hun),이종규(Lee, Jong Kyu) 한국콘크리트학회 2014 한국콘크리트학회 학술대회 논문집 Vol.2014 No.5

Identification of Leukocyte-Specific Protein 1-Positive Cells: A Clue to the Cell of Origin and a Marker for the Diagnosis of Dermatofibroma

( Sang Yun Jin ),( Jong Sun Choi ),( Yoon La Choi ),( Sang Yun Ha ),( Do Hun Kim ),( Seung Ho Lee ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.2

Background: Dermatofibroma (DF) comprises a heterogeneous group of mesenchymal tumors, with fibroblastic and histiocytic elements present in varying proportions. The cell of origin of DF has been investigated, but remains unclear. Objective: The present study attempted to investigate the expression of leukocyte-specific protein 1 (LSP1), a marker of fibrocytes, in DF. Additionally, we evaluated the effectiveness of LSP1 in the differential diagnosis of DF from dermatofibrosarcoma protuberans (DFSP). Methods: Immunohistochemical staining was performed on 20 cases of DF using antibodies against LSP1, CD68, and factor XIIIa (FXIIIa). In addition, the expression of LSP1 and FXIIIa was evaluated in 20 cases of DFSP. Results: Eighteen of 20 cases (90%) of DF stained positive for LSP1, with variation in the intensity of expression. CD68 was positive in 10 cases (50%), and FXIIIa was expressed in all cases of DF. There were differences between the regional expression patterns of the three markers in individual tumors. In contrast, only 2 of 20 cases of DFSP expressed LSP1, and none of DFSP cases stained positive for FXIIIa. Conclusion: The LSP1-positive cells in DF could potentially be fibrocyte-like cells. FXIIIa and CD68 expression suggests that dermal dendritic cells and histiocytes are constituent cells of DF. It is known that fibrocytes, dermal dendritic cells and histiocytes are all derived from CD14+ monocytes. Therefore, we suggest that DF may originate from CD14+ monocytes. Additionally, the LSP1 immunohistochemical stain could be useful in distinguishing between DF and DFSP.(Ann Dermatol 27(2) 157∼162, 2015)

대학생의 기능성 소재에 대한 지식과 기대수준에 따른 행동의도 차이

최라윤 ( La Yun Choi ),전은경 ( Eun Kyung Jeon ),유화숙 ( Hwa Sook Yoo ) 한국의류산업학회 2009 한국의류산업학회지 Vol.11 No.2

Identification of Leukocyte-Specific Protein 1-Positive Cells: A Clue to the Cell of Origin and a Marker for the Diagnosis of Dermatofibroma

( Sang Yun Jin ),( Jong Sun Choi ),( Yoon La Choi ),( Sang Yun Ha ),( Do Hun Kim ),( Seung Ho Lee ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.3

Background: Dermatofibroma (DF) comprises a heterogeneous group of mesenchymal tumors, with fibroblastic and histiocytic elements present in varying proportions. The cell of origin of DF has been investigated, but remains unclear. Objective: The present study attempted to investigate the expression of leukocyte-specific protein 1 (LSP1), a marker of fibrocytes, in DF. Additionally, we evaluated the effectiveness of LSP1 in the differential diagnosis of DF from dermatofibrosarcoma protuberans (DFSP). Methods: Immunohistochemical staining was performed on 20 cases of DF using antibodies against LSP1, CD68, and factor XIIIa (FXIIIa). In addition, the expression of LSP1 and FXIIIa was evaluated in 20 cases of DFSP. Results: Eighteen of 20 cases (90%) of DF stained positive for LSP1, with variation in the intensity of expression. CD68 was positive in 10 cases (50%), and FXIIIa was expressed in all cases of DF. There were differences between the regional expression patterns of the three markers in individual tumors. In contrast, only 2 of 20 cases of DFSP expressed LSP1, and none of DFSP cases stained positive for FXIIIa. Conclusion: The LSP1-positive cells in DF could potentially be fibrocyte-like cells. FXIIIa and CD68 expression suggests that dermal dendritic cells and histiocytes are constituent cells of DF. It is known that fibrocytes, dermal dendritic cells and histiocytes are all derived from CD14+ monocytes. Therefore, we suggest that DF may originate from CD14+ monocytes. Additionally, the LSP1 immunohistochemical stain could be useful in distinguishing between DF and DFSP. (Ann Dermatol 27(2) 157∼162, 2015)