Muscle-derived Stem Cells Differentiate into Functional Smooth Muscle Cells for Ureter Tissue Engineering: An Experimental Study

Zhan-Kui Zhao,Hong-Lian Yu,Fei Xiao,Shi-Wen Li,Wen-Biao Liao,Kai-Liang Zhao 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.3

We assessed the ability of muscle-derived stem cells (MDSC) to differentiate into smooth muscle cells (SMC) and their potential to promote the regeneration of smooth muscle with a vessel extracellular matrix (VECM)for tissue engineering of the ureter. MDSC were isolated,proliferated, and identified by flow cytometry. SMC phenotype differentiation was induced with a smooth muscle induction medium. Gene expression was evaluated by real-time quantitative polymerase chain reaction (PCR)and Western blot studies. The VECM was obtained by a decellularization process, and cytotoxic effects were evaluated by exposing the induced cells to a VECM extract. The induced cells were seeded onto VECM in vitro for 1 week, and then the compound grafts were used for ureter reconstitution in vivo. The grafts were obtained for histological studies at 2, 4, 8, and 16 weeks post-operation. Intravenous urography was used to evaluate renal function and ureteral patency. Flow cytometry demonstrated that the MDSC expressed Sca-1 and desmin, but did not express CD45. After induction, SMC phenotype gene expression was confirmed in the induced cells by real-time quantitative PCR and Western blot studies. VECM exhibited a nontoxic effect on the induced cells in vitro. At 16 weeks postoperation,a histological evaluation showed that multilayered urothelium and organized muscle fiber bundles had formed in the grafts. Intravenous urography demonstrated no evidence of ureteral stricture or hydroureteronephrosis. These results demonstrate that MDSC can be induced into SMC and that this was useful for promoting regeneration of smooth muscles for ureter tissue engineering. We assessed the ability of muscle-derived stem cells (MDSC) to differentiate into smooth muscle cells (SMC) and their potential to promote the regeneration of smooth muscle with a vessel extracellular matrix (VECM)for tissue engineering of the ureter. MDSC were isolated,proliferated, and identified by flow cytometry. SMC phenotype differentiation was induced with a smooth muscle induction medium. Gene expression was evaluated by real-time quantitative polymerase chain reaction (PCR)and Western blot studies. The VECM was obtained by a decellularization process, and cytotoxic effects were evaluated by exposing the induced cells to a VECM extract. The induced cells were seeded onto VECM in vitro for 1 week, and then the compound grafts were used for ureter reconstitution in vivo. The grafts were obtained for histological studies at 2, 4, 8, and 16 weeks post-operation. Intravenous urography was used to evaluate renal function and ureteral patency. Flow cytometry demonstrated that the MDSC expressed Sca-1 and desmin, but did not express CD45. After induction, SMC phenotype gene expression was confirmed in the induced cells by real-time quantitative PCR and Western blot studies. VECM exhibited a nontoxic effect on the induced cells in vitro. At 16 weeks postoperation,a histological evaluation showed that multilayered urothelium and organized muscle fiber bundles had formed in the grafts. Intravenous urography demonstrated no evidence of ureteral stricture or hydroureteronephrosis. These results demonstrate that MDSC can be induced into SMC and that this was useful for promoting regeneration of smooth muscles for ureter tissue engineering.

BIODIESEL PRODUCTION FROM WASTE COOKING OIL CATALYZED BY SOLID SUPERACID SO₄<SUP>2-</SUP>/TiO₂/La<SUP>3+</SUP> UNDER LOW PRESSURE

Kui WANG,Jian Chun JIANG,Zhan SI 한국신재생에너지학회 2012 AFORE Vol.2012 No.-

The Protective Effects of Phenolic Constituents from Gastrodia elata on the Cytotoxicity Induced by KCl and Glutamate

Zhan-Bo Huang,Zhe Wu,Fa-Kui Chen,Li-Bo Zou 대한약학회 2006 Archives of Pharmacal Research Vol.29 No.11

Seven phenolic compounds (1-7) were isolated from the tubers of Gastrodia elata. Their structures were elucidated on the basis of MS and NMR spectral data. p-Ethoxymethyl phenyl-O-β- D-glucoside (1) was proved to be a new compound, with N-(p-hydroxybenzyl)-adenosine (7) isolated from this plant for the first time. In this study, the protective effects of the six constituents (1-6) on PC12 cells against the cytotoxicity induced by KCl and glutamate were also investigated. The viability of the PC12 cells was significantly enhanced by pretreatment with the six phenolic constituents.

The Protective Effects of Phenolic Constituents from Gastrodia elata on the Cytotoxicity Induced by KCI and Glutamate

Huang, Zhan-Bo,Wu, Zhe,Chen, Fa-Kui,Zou, Li-Bo The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.11

Seven phenolic compounds (1-7) were isolated from the tubers of Gastrodia elata. Their structures were elucidated on the basis of MS and NMR spectral data. p-Ethoxymethyl phenyl-O-${\beta}$-D-glucoside (1) was proved to be a new compound, with N-(p-hydroxybenzyl)-adenosine (7) isolated from this plant for the first time. In this study, the protective effects of the six constituents (1-6) on PC12 cells against the cytotoxicity induced by KCI and glutamate were also investigated. The viability of the PC12 cells was significantly enhanced by pretreatment with the six phenolic constituents.

Genetic-Algorithm-Based Optimization of Dual Stator PM Vernier Machine with Spoke and HTS Bulks

Jing Libing,Kui Zhangtao,Yang Kun,Min Zeyu,Su Zhan,Wang Youzhong 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.5

Permanent magnet vernier machine (PMVM) has low speed and high torque characteristics. However, conventional single-rotor single-stator PMVM cannot meet the requirements of a high power factor. In this paper, a dual stator permanent magnet vernier machine (DS-PMVM) with spoke array and high-temperature superconducting (HTS) bulks is proposed to improve the power factor of the PMVM. This model adds HTS bulks between the modulation teeth to effectively suppress the magnetic field penetration, thereby reducing the magnetic flux leakage. A parametric model of DS-PMVM with 16 pole pairs PM rotor and 2 pole pairs armature field is established., and some key parameters are optimized by the genetic algorithm. The electromagnetic performance of the proposed DS-PMVM is verified by comparing with the conventional machine through the finite element simulation, which shows that the torque density of the proposed machine can reach 47.4kN m/m3 and the power factor of 0.946 under the same conditions.

Prediction of bolted joint looseness based on feature weighted-multiple kernel support vector machine

Chuanbo Liu,Yanzhao Zhu,Kui Zhan,Shenghui Guo,Kang Liu 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.6

Given that traditional theoretical diagnosis is difficult to use for the accurate prediction of the looseness of bolted joints, this paper will use the support vector machine (SVM) method to solve this problem. The paper establishes a database by studying the loosening mechanism of bolted joints and by collecting the connection information of the fastening points of the vehicle chassis. Key influence features are determined by the information gain value. The average prediction accuracy of the standard SVM model is 76.94 %, which is better than the theoretical diagnosis method. To achieve optimization, the feature weighted support vector machine and the multiple kernel function support vector machine (MKL SVM) are established, and the average prediction accuracy is 82.18 % and 84.19 %, respectively. The fusion weighted support vector machine (FW-MKL SVM) is built by combining the advantages of two weighted optimization SVMs. The average prediction accuracy is 94.5 %, which meets practical engineering requirements.

2017 Multimodality Appropriate Use Criteria for Noninvasive Cardiac Imaging: Expert Consensus of the Asian Society of Cardiovascular Imaging

ASCI Practice Guideline Working Group,백경민,김정아,최연현,Sim Kui Hian,John Hoe,홍유진,김성목,김태훈,김영진,김윤현,Sachio Kuribayashi,이종민,Lilian Leong,임태환,Bin Lu,박재형,Hajime Sakuma,양동현,Tan Swee Yaw,Yung-Liang Wan,Zhaoqi Zhan 대한영상의학회 2017 Korean Journal of Radiology Vol.18 No.6

In 2010, the Asian Society of Cardiovascular Imaging (ASCI) provided recommendations for cardiac CT and MRI, and this document reflects an update of the 2010 ASCI appropriate use criteria (AUC). In 2016, the ASCI formed a new working group for revision of AUC for noninvasive cardiac imaging. A major change that we made in this document is the rating of various noninvasive tests (exercise electrocardiogram, echocardiography, positron emission tomography, single-photon emission computed tomography, radionuclide imaging, cardiac magnetic resonance, and cardiac computed tomography/angiography), compared side by side for their applications in various clinical scenarios. Ninety-five clinical scenarios were developed from eight selected pre-existing guidelines and classified into four sections as follows: 1) detection of coronary artery disease, symptomatic or asymptomatic; 2) cardiac evaluation in various clinical scenarios; 3) use of imaging modality according to prior testing; and 4) evaluation of cardiac structure and function. The clinical scenarios were scored by a separate rating committee on a scale of 1–9 to designate appropriate use, uncertain use, or inappropriate use according to a modified Delphi method. Overall, the AUC ratings for CT were higher than those of previous guidelines. These new AUC provide guidance for clinicians choosing among available testing modalities for various cardiac diseases and are also unique, given that most previous AUC for noninvasive imaging include only one imaging technique. As cardiac imaging is multimodal in nature, we believe that these AUC will be more useful for clinical decision making.