Remarkably improved interfacial adhesion of UHMWPE fibers reinforced composite by constructing a three-dimensional stacked nanoparticles structure at interphase

Kui Wang,Lu Shen,Rong Lu,Zhao Yang,Zongbao Qin 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.110 No.-

Ultrahigh molecular weight polyethylene (UHMWPE) fibers have an ultralow interfacial property becauseof their extraordinarily smooth and nonpolar surfaces, and this property is a persistent problem inUHMWPE fiber-reinforced composites. In this investigation, a novel one-step and mild strategy forenhancing the interfacial property of UHMWPE fiber-reinforced composites was reported. In this strategy,a biomimetic layer with abundant stacked and three-dimensional nanoparticles formed on fiberssurface through the synergistic effect of polydopamine and 3-aminopropyltriethoxysilane. Owing tothe unique three-dimensional structure and sufficient polar groups on the fibers surface, surface roughnessand surface energy significantly increased, and wettability improved. Consequently, the interfacialshear strength (IFSS) of the fibers exhibited a 407.8% increase (8.36 MPa) relative to that of untreatedUHMWPE (2.05 MPa) because of these synergistic effects. Further analysis indicated that the failuremechanism of the fibers with the highest IFSS was the transformation from adhesive failure to cohesivefailure and substrate failure. The mild preparation process maintained the high crystallinity and orientationof the modified UHMWPE fibers, which exhibited a high tensile force of up to 81 cN.

Changes in Gene Expression of Actinobacillus pleuropneumoniae in Response to Anaerobic Stress Reveal Induction of Central Metabolism and Biofilm Formation

Lu Li,Jiawen Zhu,Kui Yang,Zhuofei Xu,Ziduo Liu,Rui Zhou 한국미생물학회 2014 The journal of microbiology Vol.52 No.6

Actinobacillus pleuropneumoniae is an important porcinerespiratory pathogen causing great economic losses in thepig industry worldwide. Oxygen deprivation is a stress thatA. pleuropneumoniae will encounter during both early infectionand the later, persistent stage. To understand modulationof A. pleuropneumoniae gene expression in responseto the stress caused by anaerobic conditions, gene expressionprofiles under anaerobic and aerobic conditions werecompared in this study. The microarray results showed that631 genes (27.7% of the total ORFs) were differentially expressedin anaerobic conditions. Many genes encoding proteinsinvolved in glycolysis, carbon source uptake systems,pyruvate metabolism, fermentation and the electron respirationtransport chain were up-regulated. These changes ledto an increased amount of pyruvate, lactate, ethanol and acetatein the bacterial cells as confirmed by metabolite detection. Genes encoding proteins involved in cell surface structures,especially biofilm formation, peptidoglycan biosynthesisand lipopolysaccharide biosynthesis were up-regulatedas well. Biofilm formation was significantly enhancedunder anaerobic conditions. These results indicate that inductionof central metabolism is important for basic survivalof A. pleuropneumoniae after a shift to an anaerobic environment. Enhanced biofilm formation may contribute tothe persistence of this pathogen in the damaged anaerobichost tissue and also in the early colonization stage. Thesediscoveries give new insights into adaptation mechanismsof A. pleuropneumoniae in response to environmental stress.

A Survey Study of Jewelry`s pattern preference in Chinese Jade

He Kui(何魁),Zhang Zheng(張政),Kang Wei rui(康維?),Lu Bo fei(陸柏妃),Huang Hua(黃華),Zeng Guo yin(曾國銀),Hyoung, Sung Eun(邢成恩) 한국디자인학회 2015 한국디자인학회 학술발표대회 논문집 Vol.2015 No.10

Lack of Any Association of GST Genetic Polymorphisms with Susceptibility to Ovarian Cancer - a Meta-analysis

Han, Li-Yuan,Liu, Kui,Lin, Xia-Lu,Zou, Bao-Bo,Zhao, Jin-Shun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.15

Objective: Epidemiology studies have reported conflicting results between glutathione S-transferase Mu-1 (GSTM1), glutathione S-transferase theta-1 (GSTT1) and glutathione S-transferase pi-1 (GSTP1) and ovarian cancer (OC) susceptibility. In this study, an updated meta-analysis was applied to determine whether the deletion of GSTM1, GSTT1 and GSTP1 has an influence on OC susceptibility. Methods: A published literature search was performed through PubMed, Embase, Cochrane Library, and Science Citation Index Expanded database for articles published in English. Pooled odds ratios (ORs) and 95% confidence intervals (95%CIs) were calculated using random or fixed effects models. Heterogeneity between studies was assessed using the Cochrane Q test and $I^2$ statistics. Sub-group analysis was conducted to explore the sources of heterogeneity. Sensitivity analysis was employed to evaluate the respective influence of each study on the overall estimate. Results: In total, 10 published studies were included in the final analysis. The combined analysis revealed that there was no significant association between GSTM1 null genotype and OC risk (OR=1.01, 95%CI: 0.91-1.12). Additionally, there was no significant association between GSTT1 genetic polymorphisms and OC risk (OR=0.98, 95% CI: 0.85-1.13). Similalry, no significant associations were found concerning the GSTP1 rs1695 locus and OC risk. Meanwhile, subgroup analysis did not show a significant increase in eligible studies with low heterogeneity. However, sensitivity analysis, publication bias and cumulative analysis demonstrated the reliability and stability of the current meta-analysis. Conclusions: These findings suggest that GSTs genetic polymorphisms may not contribute to OC susceptibility. Large epidemiological studies with the combination of GSTM1 null, GSTT1 null and GSTP1 Ile105Val polymorphisms and more specific histological subtypes of OC are needed to prove our findings.

Effects of Florfenicol and Chromium (III) on Humoral Immune Response in Chicks

Cao, Jiyue,Li, Kui,Lu, Xiaocong,Zhao, Yaxin Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.3

One hundred and sixty day-old Hainan chicks were randomly allotted into eight pens to investigate the effect of different dietary concentrations of chromium (Cr) in the form of chromium chloride, and different dosages of florfenicol on humoral immune responses by determining antibody titers to Newcastle disease (ND) vaccines using the hemagglutination inhibition test. The results indicated that ND antibody titers were significantly higher in chicks receiving Cr at low (5 mg/kg feed) and middle (10 mg/kg feed) dose compared with the control (p<0.01). However, ND antibody titers were significantly decreased in chicks receiving Cr at a high dosage of 500 mg/kg feed (p<0.01), though the ND antibody titers of the early days (d 21 and d 28 of age) were higher than that of the control group. It is suggested that excessive Cr intake has detrimental effects on ND antibody production in chicks. No significantly lower response was measured in chicks that received florfenicol at a low dosage of 50 mg/kg feed (p>0.05), but the ND antibody titers were significantly decreased in chicks receiving 200 and 400 mg/kg feed of the drug (p<0.01). The ND antibody titers of group receiving 200 mg/kg feed of florfenicol plus 10 mg/kg Cr were slightly higher than that of the group receiving single florfenicol of 200 mg/kg although, no significant differences were observed between these two treatments. It is suggested that the humoral immune response impaired by florfenicol (200 mg/kg feed) could not be significantly reversed by Cr (10 mg/kg feed).

CT Perfusion Imaging Can Predict Patients’ Survival and Early Response to Transarterial Chemo-Lipiodol Infusion for Liver Metastases from Colorectal Cancers

Wei-Fu Lv,Jian-Kui Han,De-Lei Cheng,Chun-Ze Zhou,Ming Ni,Dong Lu 대한영상의학회 2015 Korean Journal of Radiology Vol.16 No.4

To prospectively evaluate the performance of computed tomography perfusion imaging (CTPI) in predicting the early response to transarterial chemo-lipiodol infusion (TACLI) and survival of patients with colorectal cancer liver metastases (CRLM). Computed tomography perfusion imaging was performed before and 1 month after TACLI in 61 consecutive patients. Therapeutic response was evaluated on CT scans 1 month and 4 months after TACLI; the patients were classified as responders and non-responders based on 4-month CT scans after TACLI. The percentage change of CTPI parameters of target lesions were compared between responders and non-responders at 1 month after TACLI. The optimal parameter and cutoff value were determined. The patients were divided into 2 subgroups according to the cutoff value. The log-rank test was used to compare the survival rates of the 2 subgroups. Four-month images were obtained from 58 patients, of which 39.7% were responders and 60.3% were non-responders. The percentage change in hepatic arterial perfusion (HAP) 1 month after TACLI was the optimal predicting parameter (p = 0.003). The best cut-off value was -21.5% and patients who exhibited a ≥ 21.5% decrease in HAP had a significantly higher overall survival rate than those who exhibited a < 21.5% decrease (p < 0.001). Computed tomography perfusion imaging can predict the early response to TACLI and survival of patients with CRLM. The percentage change in HAP after TACLI with a cutoff value of -21.5% is the optimal predictor.

Silencing of PDK1 Gene Expression by RNA Interference Suppresses Growth of Esophageal Cancer

Yu, Jing,Chen, Kui-Sheng,Li, Ya-Nan,Yang, Juan,Zhao, Lu Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

The current study was conducted to explore the inhibitory effects of a small interfering RNA (siRNA) on 3-phosphoinositide-dependent protein kinase 1 (PDK1) expression in esophageal cancer 9706 (EC9706) cells and the influence on their biological behavior. After transfection of a synthesized PDK1 siRNA, PDK1 mRNA and protein expression and the phosphorylation level of the downstream Akt protein were assessed using RT-PCR and Western blot analysis. Proliferation, apoptosis, cell invasion and in vivo tumor formation capacity were also investigated using MTT, flow cytometry, Transwell invasion trials, and nude mouse tumor transplantion, respectively. PDK1 siRNA effectively suppressed PDK1 mRNA and protein expression, and down-regulated the phosphorylation level of the Akt protein in the EC9706 cells (P < 0.05). It also inhibited cell proliferation and invasion, and promoted apoptosis; such effects were particularly obvious at 48 h and 72 h after transfection (P < 0.05). Growth of transplanted tumors was inhibited in nude mice, with decreased PDK1 expression in tumor tissues. PDK1 may be closely correlated with proliferation, apoptosis and invasion of esophageal cancer cells and thus may serve as an effective target for gene therapy.

Antimicrobial Aflatoxins from the Marine-Derived Fungus Aspergillus flavus 092008

Hui Wang,Wei-Ming Zhu,Zhenyu Lu,Hai-Jun Qu,Peipei Liu,Chengdu Miao,Tonghan Zhu,Jing Li,Kui Hong 대한약학회 2012 Archives of Pharmacal Research Vol.35 No.8

A new aflatoxin, aflatoxin B2b (1), together with six known compounds, were isolated from the marine-derived fungus Aspergillus flavus 092008 endogenous with the mangrove plant Hibiscus tiliaceus (Malvaceae). The structure of 1 was determined by the spectroscopic and chemical methods. Compound 1 exhibited a moderate antimicrobial activity against Escherichia coli, Bacillus subtilis and Enterobacter aerogenes, with MIC values of 22.5, 1.7 and 1.1 μM, respectively. Compound 1 also showed a weak cytotoxicity against A549, K562 and L-02 cell lines, with IC50 values of 8.1, 2.0 and 4.2 μM, respectively. The results showed that hydration and hydrogenation of Δ8-double bond significantly reduces the cytotoxicity of aflatoxins, while the esterification at C-8 increases the cytotoxicity.

A Resistive Memory in Semiconducting BiFeO₃ Thin‐Film Capacitors

Jiang, An Quan,Wang, Can,Jin, Kui Juan,Liu, Xiao Bing,Scott, James F.,Hwang, Cheol Seong,Tang, Ting Ao,Lu, Hui Bin,Yang, Guo Zhen WILEY‐VCH Verlag 2011 ADVANCED MATERIALS Vol.23 No.10

<P><B>A ferroelectric‐resistive random access memory consisting of a conductive BiFeO<SUB>3</SUB></B> epitaxial thin film with a unipolar diode current modulated by electric polarization orientation is reported. This device has a memory that lasts for months, a sufficiently high on current and on/off ratio to permit ordinary sense amplifiers to measure “1” or “0”, and is fully compatible with complementary metal‐oxide semiconductor processing. </P>

Changpingibacter yushuensis gen. nov., sp. nov., isolated from fluvial sediment in Qinghai Tibet Plateau of China

Jiao Yifan,Zhang Sihui,Yang Jing,Lai Xin-He,Dong Kui,Cheng Yanpeng,Xu Mingchao,Zhu Wentao,Lu Shan,Jin Dong,Pu Ji,Huang Ying,Liu Liyun,Wang Suping,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.2

Two facultatively anaerobic, short rod-shaped, non-motile, Gram-stain-positive, unknown bacterial strains (JY-X040T and JY-X174) were isolated from fluvial sediments of Tongtian River in Yushu Tibetan Autonomous Prefecture, Qinghai province, China. Cells formed translucent, gray, round and convex colonies, with a diameter of less than 0.5 mm after 5 days of incubation at 30°C on brain heart infusion-5% sheep blood agar. The 16S rRNA gene sequence similarity between strain JY-X040T and Fudania jinshanensis 313T is 93.87%. In the four phylogenetic trees constructed based on the 16S rRNA gene and 423 core genes, the two isolates form an independent branch, phylogenetically closest to F. jinshanensis 313T, but could not be classified as a member of the genus Fudania or any other genus of the family Arcanobacteriaceae. The DNA G + C content of strain JY-X040T was 57.8%. Calculation results of average nucleotide identity, digital DNADNA hybridization value and amino acid identity between strain JY-X040T and F. jinshanensis 313T are 69.9%, 22.9%, and 64.1%. The major cellular fatty acids were C16:0 (23%) and C18:1ω9c (22%). The cell-wall peptidoglycan type was A5α (L-Lys-L-Ala-L-Lys-D-Glu). The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and four unidentified components. The whole-cell sugars contained rhamnose and ribose. MK-10(H4) was the sole respiratory quinone. The minimum inhibitory concentration of streptomycin was 32 μg/ml. All physiological, biochemical, chemotaxonomic and genomic characteristics support that strains JY-X040T and JY-X174 represent members of a novel species in a new genus, Changpingibacter yushuensis gen. nov., sp. nov. The type strain is JY-X040T (GDMCC 1.1996T = KCTC 49514T).