멕시코와 네팔에서 도입한 고추 유전자원의 풋마름병 및 역병 저항성

고보환,김정훈,전수경,이지선,김병수 慶北大學校 農業科學技術硏究所 2005 慶北大農學誌 Vol.23 No.-

Above 130 accessions of pepper consisting of 50 introductions from Mexico and Nepal, and resistant and susceptible controls were tested for resistance to bacterial wilt and to Phytophthora root rot at seedling stage by artificial inoculation. As the results, KC897, KC939, KC936 were newly found resistant to bacterial wilt in addition to already known resistance sources such as KC126, KC350, KC351, KC353. No new sources of resistance to Phytophthora root rot were found among the introductions from Mexico and Nepal.

참다래잼의 제조와 품질특성

고정삼,김지용,김용철,강순선 濟州大學校 亞熱帶農業硏究所 1999 亞熱帶農業硏究 Vol.16 No.-

초록 제주산 참다래의 화학적 분석을 통하여 가공적성을 구명하고 잼 생산을 위한 최적 제조조건을 검토하였다. 참다래의 수분 함량은 82.84%이었으며, 당함량은 12.08%로 비교적 높은 편이었다. 가용성고형물은 14.80이었고 산 함량은 1.33%이었으며, 비타민 C 함량은 27.47%/100g로 높은 편이었다. 관능평가에 의한 상품은 원료 300g에 첨가하는 당농도를 60%로 하며, 당을 100%로 하였을 경우 설탕 : 물엿 : 올리고당(60 : 30 : 10)으로 하며, 여 기에 펙틴 1%와 청주 10㎖를 첨가하는 경우 잼의 물성과 변향을 억제하기 위하여 바람직하 였다. 시제품의 관능평가 결과시판하고 있는 유산 제품보다 기호성이 높아 개발가티가 있는 것으로 판단되었으며, 열탕에서 5분간 처리한 시제품은 30℃에서 한달간 저장 중 미생물 증 식이 인식되지 않아 유통 중에 문제가 없을 것으로 보인다. Abstract Chemical analysis and optimum processing conditions of kiwiifruit jam were investigated. Soluble solids, acid content and vitamin C of kiwiifruit was 14.80, 1.33% and 27.47mg/100g, respectively. Moisture content and total sugar of fruits was 82.84% and 12.08%. In processing of kiwiifruit jam, the ratio of sucrose : maltose syrup : oligo sugar (60 : 30 : 10) was the best in addition of sugar source 60%(w/w) in total. Otherwise, the addition of 1% pectin and 10ml of rice wine were recommended for texture and masking of off-flavor. Kiwiifruit jam prepared in this experiment was better than that of commercial other products in sensory evaluation, and microbial growth of this product was not recognized for one month at 30℃ with treatment the products for 5min in boiling water.

고등학교 지구과학Ⅱ 교과서의 ‘화성암의 조직’에 대한 내용 분석

고정선,윤성효,한종수 부산대학교 과학교육연구소 2007 교사교육연구 Vol.46 No.2

이 연구는 7차 고등학교 공통과학 교과서의 내용 중 화성암 암석의 조직 개념에 대한 본문진술과 제시된 그림 및 도표들을 분석하고 오개념 유발과 관련하여 문제점을 지적하고 그 개선방안을 모색하였다. 과학교과서의 개념진술이 정확하지 않아 학습자들에게 오개념을 유발하거나 재강화할 가능성이 있는 여러 진술문을 발견하였고 학생의 과학적 개념형성에 부적절한 그림이나 도표들도 발견되었다. 이들은 화성암 조직 분류체계의 기준의 혼란에 관련된 것들이다. 이들 개념진술을 대체할 개선방안들을 제시하였다. The purpose of this study is to analyze the seventh curriculum textbooks of high school Earth Science-II courses in relation to the igneous rock texture concept and find incorrect descriptions of that concept and then suggest some improved schemes. Some incorrect descriptions, pictures and tables on the igneous rock texture concept were found in texts. They might contribute to forming and/or reinforcing misconceptions about the confusion of criterion about the igneous rock texture. Therefore some improved schemes were suggested.

부산 지역의 야외 지질 학습 자료 개발과 그 효과

장정일,고정선,윤성효 부산대학교 사범대학 과학교육연구소 2002 科學敎育硏究報 Vol.29 No.-

Learning materials for the field geological practices along the level-differentiated inquiry learning that considered learning ability of students were developed, and the effects of self-directed field geology program on the learning achievements and self-directed learning characteristics tried to examine. As for the field geological learning sites, the regions chosen Dadaepo and Songdo area in the southern part of Busan city, due to fresh outcrops and accessibility for the students. As for developed learning materials, it is considered to help in a learning achievements and self-directed learning ability.

제7차 교육과정의 과학교과서 지질단원에 대한 연계성 분석

윤성효,박기현,고정선 부산대학교 사범대학 과학교육연구소 2005 科學敎育硏究報 Vol.32 No.-

Using the task analysis technique, this study analyzes the contents of study and inquiry activities of geo-science units in twelve textbooks in total, which include eight elementary level textbooks from the third to sixth grades, three middle school level textbooks, one high school level science book. After choosing several main concepts, this paper analyzes the connection and hierarchy among them and presents the hierarchical structure as a diagram. The conclusions drawn from this study are as follows: First, from the analysis of studying content on geo-science unit, in the science education content from e third to tenth grades, the articulation of the major concepts, such as the inner part of the earth, rocks, plate tectonics, volcanic activities and earthquakes, layers and fossils, and gen-structure, are well associated with one another; on the other hand, unnecessary repetition and discontinuation appear in the mineral and gen-history sections. Overall, from simpler subjects, such as those related to the daily life, to the more complex scientific ones, the connection of contents is well maintained, which corresponds to the principle of organizing contents in the 7th curriculum. Second, the proportion of inquiry activities from analyzing inquiry content relating geo-science, is as follows: at the elementary school level, a total of thirty-one inquiry activities composed of observation (51.6%) and experiment (19.4%); at the middle school level, a total of twenty-seven inquiry activities consisted of data analysis (47.1%), experiment (25.9%) and observation (18.5%); at the high school level, these comprised data analysis (47.1%), discussion(17.6%), and follow-up (11.8%). Such findings show that while elementary schools focus on the observation of geological phenomena around us, middle schools on the data analysis, experiment, and observation, and high schools on particularly on the data analysis. Third, one of the important principles of the seventh curriculum is to compile textbooks in order to maintain the articulation between contents accordingly from the third grade to the tenth grade. The seventh curriculum emphasizes teaching and learning according to this code, and this paper presents the hierarchical structure as a diagram showing the flow among main learning points, It would contribute to improve instruction of science teachers in elementary, middle and high schools by helping to fully understand the hierarchical structure of the geo-science unit. therefore It will facilitates the application and settlement of the seventh curriculum.

부산시 고등학생들의 환경에 대한 인식정도와 영향요인 분석

최인영,윤성효,고정선 부산대학교 사범대학 과학교육연구소 2004 科學敎育硏究報 Vol.31 No.-

The purpose of this study was to identify the degree of recognition of the high school students about environmental and influential factors on it, selecting two regular schools and three vocational schools among the high schools in Busan area, questionnairing one class in the 10th, 11th, 12th grades of each school and analyzing the result. The result of analyzing the degree of knowledge, practice and recognition of seriousness concerning environment according to grade and gender is that the significant difference has been found in the degree of practice. The result of analyzing them according to maternal academic background is that the significant difference has been found in the degree of knowledge and a living standard and the style of housing have no effect on the environmental education, In the survey according to family style, it is shown that the students 'who live with their parents' recognize the seriousness of environment more. In the survey according to the experience of having the subject 'environment' in their school curriculum or not, it is shown that the significant difference has been found in the degree of knowledge. As the source of information on environment, mass media such as TV, magazines and newspapers have a great impact on the students. As the significant difference has been found in the degree of knowledge, practice, the recognition of seriousness from the viewpoint of the analysis on the survey according to the degree of the recognition on the necessity of the environmental preservation, it is shown that the more the students feel the necessity of environmental preservation, the higher the degree of knowledge, practice and recognition of seriousness on environment becomes, In the survey according to the kind of school, the significant difference has been found in the degree of knowledge, In the survey according to the degree of maternal concern on environment it is shown that the more concerns the mothers have, the more the students put what they learn into practice, In the survey according to the kinds of school, the significant difference has been found in the degree of knowledge. As the result of multiple regression analysis is summarized as below. 1. The variables influencing the environmental knowledge are the experience of having the class, 'environment', the recognition of the necessity of the environmental preservation and maternal academic background. 2. The elements influencing the degree of recognition of seriousness about environmental contamination are gender, the experience of having a class, 'environment' or not and the necessity of the environmental education. 3. The elements influencing the degree of practice are grade, information on environment, and maternal concern.

HPLC에 의한 柑橘中의 遊離糖 定量

金瑛淑,康順善,高正殷,柳長杰 제주대학교 1985 논문집 Vol.21 No.-

HPLC에 의한 分析에서 가장 많은 經費를 부담케하는 것중의 하나가 容媒이기 때문에 이와같은 점에서 柑橘汁중의 遊離糖 分析을 하는데 관련되는 몇가지 조건을 檢討한 結果는 다음과 같았으며 사용 機器는 Waters 회사 제품의 본체에 μBondapak NH₂column, RI detector (x8)이었고 용매로는 acetonitrile/H₂O system을 사용했다. 1. 75%의 acetonitrile 농도에서 1.0㎖/min의 유속이 容媒를 절약하는데 가장 경제적인 조건이었다. 2. 시료중의 불순물을 제거하기 위해서 Sep-Rak C?? cartridge를 사용할 경우 糖시료를 한번 통과시킨 뒤에 4㎖의 H₂O로 세척하면 재사용이 가능했다. H₂O로 세척된 cartridge에는 다음 시료를 3㎖ 통과 시킨뒤에 나오는 시료액을 injection sample로 사용하면 안전하다. 3. 시료주입량을 보면 fructose, glucos,sucrose 모두 140㎍까지 주입해도 직선상의 결과를 얻을 수 있었다. 4. 본 실험 조건의 HPLC에 의한 糖분석에서 glucose와 mannitol의 분리가 좋지 않으므로 내부표준물질로 mannitol을 사용하는 것은 부적당하다. In order to minimize the cost of sugar analysis of mandarin juice by HPLC, some conditions related were discussed using Waters, M246 HPLC main body with R-401 RI-detector(x8), M510 pumping system, U6K injector, M730 Data Module, μBondapak NH₂column (3.9mm*30cm), Sep-Pak C?? cartridge and acetonitrile/H₂O solvent system. 1) The most suitable conditions to save acetonitrile solvent were obtained at 1.0ml/min of the flow rate of 75% acetonitrile in H₂O. 2) The Sep-Pak C?? cartridge, used once, and be washed out by using 4ml of H₂O under the conditions of the present study. When the washed Sep-Pak cartridges wetted with H₂O were passed through with 3ml of the new sugar sample, there was no dilution effect found and nearly 100% sugar yield in the filtrate. 3) Up to 140㎍ of sugars (fructose,glucose, sucrose) there was good linear relationship bwteen the injected amounts and the analyzed values. 4) It was found that mannitol can not be used as the internal sugar standard because of overlapping with glucose chromatogram in HPLC under the present conditions.

The Geochemistry of Yuksipryeong Two-Mica Leucogranite, Yeongnam Massif, Korea

Jeong Seon Koh(고정선),Sung Hyo Yun(윤성효) 한국암석학회 2003 암석학회지 Vol.12 No.3

Lack of Mitochondrial DNA Sequence Divergence between Two Subspecies of the Siberian Weasel from Korea: Mustela sibirica coreanus from the Korean Peninsula and M. s. quelpartis from Jeju Island

Koh, Hung-Sun,Jang, Kyung-Hee,Oh, Jang-Geun,Han, Eui-Dong,Jo, Jae-Eun,Ham, Eui-Jeong,Jeong, Seon-Ki,Lee, Jong-Hyek,Kim, Kwang-Seon,Kweon, Gu-Hee,In, Seong-Teak The Korean Society of Systematic Zoology 2012 Animal Systematics, Evolution and Diversity Vol.28 No.2

The objective of this study was to determine the degree of mitochondrial DNA (mtDNA) divergence between two subspecies of $Mustela$ $sibirica$ from Korea ($M.$ $s.$ $coreanus$ on the Korean Peninsula and ($M.$ $s.$ $quelpartis$ on Jeju Island) and to examine the taxonomic status of ($M.$ $s.$ $quelpartis$. Thus, we obtained complete sequences of mtDNA cytochrome $b$ gene (1,140 bp) from the two subspecies, and these sequences were compared to a corresponding haplotype of ($M.$ $s.$ $coreanus$, downloaded from GenBank. From this analysis, it was observed that the sequences from monogenic ($M.$ $s.$ $quelpartis$ on Jeju Island were identical to the sequences of four ($M.$ $s.$ $coreanus$from four locations across the Korean Peninsula, and that the two subspecies formed a single clade; the average nucleotide distance between the two subspecies was 0.26% (range, 0.00 to 0.53%). We found that the subspecies $quelpartis$ is not genetically distinct from the subspecies $coreanus$, and that this cytochrome $b$ sequencing result does not support the current classification, distinguishing these two subspecies by pelage color. Further systematic analyses using morphometric characters and other DNA markers are necessary to confirm the taxonomic status of ($M.$ $s.$ $quelpartis$.

Lack of genetic divergence between Mogera wogura coreana from Korea and M. w. robusta from Northeastern China and adjacent Russia (Soricomorpha: Mammalia), reexamined from 12S rRNA and cytochrome b sequences

Koh, Hung Sun,Jang, Kyung Hee,Han, Eui Dong,Jo, Jae Eun,Jeong, Seon Ki,Ham, Eui Jeong,Lee, Jong Hyek,Kim, Kwang Seon,In, Seong Teek,Kweon, Gu Hee The Korean Society for Integrative Biology 2012 Animal cells and systems Vol.16 No.5

To reexamine taxonomic status of endemic Mogera wogura coreana from Korea, we first obtained partial 12S rRNA sequences (893 bp) and complete cytochrome b gene sequences (1140 bp) of this subspecies, and these sequences and partial cytochrome b sequences (402 bp) were compared to the corresponding haplotypes of M. wogura from East Asia, obtained from GenBank. The one of three 12S rRNA haplotypes in M. w. coreana was identical to one 12S rRNA haplotype of M. w. robusta from East Asia: 10 complete and 13 partial cytochrome b haplotypes of M. w. coreana formed a single clade with one complete and four partial cytochrome b haplotypes of M. w. robusta, respectively. We considered that M. w. coreana from Korea is an endemic subspecies with only morphological differences, although it is necessary to reexamine the subspecies status of M. w. coreana. Additionally, in the 12S rRNA and complete cytochrome b sequences, M. wogura from Japan was distinct from the two continental subspecies of M. w. coreana and M. w. robusta with average distances of 1.76 and 5.65%, respectively; insular M. wogura, with within-group distances of 2.09 and 4.38%, respectively, was also genetically more divergent than the mainland M. wogura, with within-group distances of 0.08 and 0.57%, respectively. Thus, we considered that insular M. wogura of Japan dispersed into neighboring East Asian continent, which is opposite to the traditional hypothesis on the origin of Japanese M. wogura.