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고정삼,이계호,박성오 한국농화학회 1976 Applied Biological Chemistry (Appl Biol Chem) Vol.19 No.3
In order to utilize the agricultural waste products, two strains of mold producing powerful cellulolytic enzyme were screened from various soils, composts, rotten wood and others. The optimum condition of cellulase production was studied. The results obtained were summarized as follows. 1. Two strains of mold which showed remarkably high cellulolytic activity were identified as Aspergillus niger-SM 6 and Trichoderma viride-SM 10. 2. The highest cellulase production was obtained at pH 5.0-6.0 in 5 days. 3. Cellulase production in strain Aspergillus niger-SM 6 increased with the addition of C.M.C., (NH₄)₂SO₄, C.S.L., orange peel powder and rice hull. The rice hull, treated with 3N NaOH at 120℃ for 15 min. and neutralized with various acids, was used. Up to 50% of wheat bran could be substituted by the treated rice hull without any-decrease of cellulase activity. 4. In the strain of Trichoderma viride-SM 10, cellulase production increased with the addition of C.M.C., NH₄NO₃, Vitamin-free casamino acid and orange peel powder, while the other carbon, nitrogen, phosphate sources, natural nutrients and organic substances gave no remarkable effect.
고정삼,이계호 한국농화학회 1975 Applied Biological Chemistry (Appl Biol Chem) Vol.18 No.3
In order to utilize the agricultural waste products for animal feeds, studies have been made concerning the production of cellulose of Irpex lacteus and its properties of crude enzyme, and summarized as follows. 1. At the production of cellulose, the culture of wheat bran added with rice bran was more active than any other cultures. 2. The optimum incubation time is 5 days in shaking culture. 3. The optimum condition of reaction in saccharification with CMC were obtained the following results. 1) The optimum pH was within the range of from 3.5 to 4.0 and stable within 3.0 to 6. 0. 2) The optimum temperature was 40℃ and thermal stability was below 40℃. 3) The optimum reaction time was 2 hours. 4) The optimum crude enzyme concentration was 50%V/V and substrate concentration 1%.
고정삼,고남권,강순선 한국농화학회 1989 Applied Biological Chemistry (Appl Biol Chem) Vol.32 No.4
In order to produce clear and favorable citrus wine from Citrus unshiu produced in Cheju island, chemical and microbiological processes for alcoholic fermentation were investigated. The ratio of pressed juice passed below 100 mesh sieve and peel of mandarin orange were 55.9% and 25.6% respectively. Orange juice for fermentation source contained 8.85% total sugar, 1.43% total acid and 0.056% volatile acid. Pressed juice was adjusted to 24 degree Brix with cane sugar, and was fermented at 20℃ for one month. Starter screened and selected was Sacclzaromyces cerevisiae IAM 4274. As principal fermentation proceeded for one week, suspended solids began to precipitate slowly after then. After fermentation, clear citrus wine consisted of about 8 degree Brix residual sugar, 13.3∼14.4% ethanol, 0.78∼1.11% total acid, 0.05∼0.07% methanol and 2. 25∼3.29% extract, was obtained. Color, flavor and taste of citrus wine found good with panel test. citrus wine which was treated with fungal enzyme derived from Aspergillus niger CCM-4 was cleared much faster, and could be filtered more rapidly than the untreated. The enzyme-producing strain was isolated from field soil of Cheju island and identified.
고정삼,김지용,강문장 한국농화학회 2000 Applied Biological Chemistry (Appl Biol Chem) Vol.43 No.3
Satsuma mandarin(Citrus unshiu Marc. var. miyagawa) was stored at 3℃ and 85% relative huifidity, and then the changes of firmness, pectin- degrading enzymes activity and other physicochemical properties of citrus fruits during storage were investigated. Firmness of fruits with 2 ㎜ probe was decreased quickly from 1,176.8 g-force to 503.6 g-force, and moisture of peel and flesh were decreased from 75.3% to 74.9% and from 91.8% to 90.7% during maturation, respectively, Decay ratio was increased to 18.75% after 90 days' storage, and after then it was increased rapidly. Weight loss was increased gradually to 24.5% during long-term storage. Firmness with 2 ㎜ probe were decreased from 538.9 g-force to 336.9 g-force gradually during storage. Peel moisture was decreased from 75.8% to 72.6%, and flesh moisture was also decreased gradually from 90.3% to 88.3% during storage. Exopoly-galacturonase activity of peel and flesh were increased from 326.0 units/100g to 534.9 units/100g, and from 63.1 units/100g to 81.0 units/100g at 90 day's storage, respectively. After then, the enzyme activities were decreased form 394.0 units/100g and 38.0 units/100g, respectively. Pectinesterase activity of peel and flesh were increased form 14.4 μ㏖ to 38.8 μ㏖, and from 26.0 μ㏖ to 39.0 μ㏖ at 60 days' storage, respectively. After then, the enzyme activities were decreased to 6.0 μ㏖ and 8.2 μ㏖, respectively.
고정삼,양상호,김성학,Go, Jeong-Sam,Yang, Sang-Ho,Kim, Seong-Hak 한국식품저장유통학회 1996 한국식품저장유통학회지 Vol.3 No.2
The conditions of cold stroage of Citrus unshiu Marc. var. okitsu produced in Cheju were investigated. The changes of peel moisture contents soluble solids, total sugar, vitamin C and density were slightly occured, and decay ratio was below 20% on keeping freshness relatively till late of March during cold storage. After thats decayed citrus fruits were increased gradually mainly from cold injury with lower temperature and high humidity. The loss of fruit weight, decrease of fruit hardness, and decrease of acid content were occured gradually during cold stroage. Because of the difficulty of long term storage for Citrus unshiu, the conditions and periods of cold storage would be determined after considering the physicochemical properties of fruits every year.