Effects of Interleukin-1β, Tumor Necrosis Factor-α and Interferon-γ on the Nitric Oxide Production and Osteoclast Generation in the Culture of Mouse Bone Marrow Cells

Kwon, Young-Man,Kim, Se-Won,Ko, Seon-Yle The Korean Academy of Oral Biology 2006 International Journal of Oral Biology Vol.31 No.2

Nitric oxide(NO) is a labile, uncharged, reactive radical that functions as a sensitive mediator of intercellular communication in diverse tissues. It has been reported that NO is produced by osteoblast and these results may suggest that NO is integrally involved in the regulation of osteoclast formation and osteoclast resorption activity by osteoblastic cells. We examined the effect of cytokines on NO release by mouse bone marrow cell. We also examined the effects of cytokokines and sodium nitroprusside(SNP) on the formation of osteoclast-like cell from mouse bone marrow cells in culture. Cytokines stimulated NO production of mouse bone marrow cells, and N-nitro-L-arginine methyl ester, a specific inhibitor of NO synthase, suppressed the cytokine-induced NO production. SNP showed dual action in the generation of osteoclasts. The addition of 30μM SNP inhibited the formation of tartrate resistant acid phosphatase(TRAP)(+) multinucleated cell, whereas lower concentration(3μM) of SNP enhanced it. Althogh the precise action of NO remains to be elucidated in detail, the action of NO in osteoclast generation in our studies seems to be associated, at least in part, with bone matabolism and bone pathophysiology.

청국장의 제조방법에 따른 향미 증진 효과

고한수,조대희,황성연,김영만 한국식품영양학회 1999 韓國食品營養學會誌 Vol.12 No.1

청국장 제조를 위한 분리균은 포자형성율이 높은 Bacillus subtilis로 대사력이 활발한 대수증식기의 것을 콩에 1,000CFU/g 이상의 균수를 접종하여 40℃와 상대습도 90%에서 배양하면 발효가 빠르게 진행되었다. 발효가 종료되면 포자형성율이 95% 이상 되어야 저온의 후숙과정에서 재번식이 쉽게 일어나지 않았으며 발효취는 발효 중에서만 형성되었다. 중립콩과 대립콩의 발효율이 가장 좋았다. 저온의 후숙과정은 5℃에서 1∼2일 후 경과하면 Bacillus subtilis는 더 이상의 증식이 일어나지 않고 발효취가 소멸되므로 저온의 유통과정을 거쳐야 불쾌취가 없는 구수한 청국장의 맛을 유지할 수 있었다. The strain isolated for making chungkuk-jang was Bacillus subtilis, which formed spore with 98% ratio. Logarithmical culture was inoculated (1,000 CFU /g) to the steamed soybeans and at the optimum fermentation conditions(40℃, RH 90%), fermentation progressed very rapidly and synchronously. Fermentation time was 24 hours on the optimum fermentation conditions. During activated fermentation, chungkuk-jang's aroma and flavor created. After finishing the fermentation, the spore forming ratio was 95% and replenishment was not occured easily during aging at the below 5℃.

마이크로센서 응용을 위한 실리콘 미세가공 기술

고희선,김영민,권대혁,도양희,이광만 제주대학교 산업기술연구소 1999 尖端技術硏究所論文集 Vol.10 No.2

Silicon micromachining technology can be used to build microsensors and actuators by using tools derived from standard IC processing. For the silicon micromachining the etching characteristics of tetramethyl ammonium hydroxide (TMAH) by dissolving silicon powder or silicic acid have been studied. The 3, 5 and 10wt.% TMAH solutions, with dissolved silicon powder (6g/l) or with dissolved silicic acid(Si(OH)4, 16g/l) were used. And ammonium peroxydisulfate (APODS, 2g/l) dissolved in the solutions were also used. The etch rates of silicon and the etched surface roughness were observed with various etching conditions. The crystallographical aspect on the crystal orientation of silicon have been studied. With sufficiently fresh solutions, these TMAH concentration and combination of etchants, provide repeatable etch properties, does not attack exposed aluminum or silicon dioxode, and provide smooth (100) silicon surfaces.

고성능 콘크리트의 항복조건에 관한 연구

고만영 제주한라대학 2003 論文集 Vol.27 No.-

This paper presents a study on the mix proportion design and failure criteria of high performance concrete. In this paper. the study is limitted to the high strength and high flowing concrete which has cylinder compressive strength of 600~800kg/cni, slump value of 20~25cm. and slump-flow value of 60~70cm. Also. an estimation of mechanical properties of high performance concrete was carried out by uniaxial test. Twenty- four cylinders were tested to study the failure criteria of high performance concrete. The conclusions obtained from this study on the mix proportion design and failure criteria of high performance concrete are as follows. (1) High performance concrete which has exellent consistency and small loss of slump and slump flow can be produced readily using mix proportion design proposed in this study. (2) It is found that the frictional angle of 30.5˚may be applicable for the failure criteria of high performance concrete.

데이터베이스의 효율적 이용을 위한 사무실 정보시스템에 관한 연구 -독일에의 수용 가능성 관점에서 본 일본 선진기업의 사무실 정보 및 커뮤니케이션 시스템 분석

고영만 韓國圖書館學會 1991 圖書館學 Vol.21 No.1

The purpose of this study is to investigate aspects of office information systems in Japanese corporations in order to understand introduction strategies and to find out whether strategy elements could be transferred to Germany. In this study, three points of view played an important role. (a) Technological and infrastructural support systems : What kind of role did the liberalization of telecommunication markets and the avaliability of computer hardware play to help develope of database industry and implement office information systems? (b) Strategies and forms of the implementation of office information system : Which strategies were successful, distribution or centralization? How were technical and organizational problems handled? (c) Process of data communication : How did the implementation of office information system change the data communication with in-house or commercial database systems? To answer these questions, this study centered around a two-step field research in Japan. (1) Screening stage : to gain a general overview of office information system in Japan. The results of this initial research have been analysed and carefully evaluated to prepare for the second and major part of field research in Japan. (2) Data collection stage : interviews with respective managers, heads offices, and data processing units in 20 enterprises. The major results of this study are as follows. Today in Japan, office information system is understood as the interconnection of the in-house and commercial database for the maximization of the use of information resource in an organization. In particular, developments in the direction of distributed systems and the "intelligent office" by means of the new information and communication technologies are very important

전기통신의 자유화가 전문정보서비스에 미치는 영향에 관한 연구

고영만 한국문헌정보학회 1993 한국문헌정보학회지 Vol.24 No.-

The use of telematics, or of data communications, for the information services is a much debated issue currently in the information science. The purpose of this study is to gain a general overview of the use of data communications in specialized information centers in order to find out the direction of development in furture.In this study, the state of the specialized information services by data communications in Korea was analysed from the following points of view: (1) What kind of role play the liberalization of telecommunication to help develope the specialized information services? (2) Which problems have happend to the specialized information services using data communications? (3) How are technical, economical, and methodical problems handled for the new services? The background research stems from interview with managers and researchers in 5 specialized information centers

마이크로센서 응용을 위한 실리콘 미세가공 기술

고희선,김영민,권대혁,도양회,이광만 濟州大學校 産業技術硏究所 1999 산업기술연구소논문집 Vol.10 No.2

Silicon micromachining technology can be used to build microsensors and actuators by using tools derived from standard IC processing. For the silicon micromachining the etching characteristics of tetramethyl ammonium hydroxide (TMAH) by dissolving silicon powder or silicic acid have been studied. The 3. 5 and l0wt.% TMAH solutions, with dissolved silicon powder (6g/l) or with dissolved silicic acid(Si(OH)_(4), 16g/1) were used. And ammonium peroxydisulfate (APODS. 2g/l) dissolved in the solutions were also used. The etch rates of silicon and the etched surface roughness were observed with various etching conditions. The crystallographical aspect on the crystal orientation of silicon have been studied. With sufficiently fresh solutions, these TMAH concentration and combination of etchants. provide repeatable etch properties. does not attack exposed aluminum or silicon dioxode. and provide smooth (100) silicon surfaces.

남자 고등학교 세팍타크로 경기의 공격기술에 관한 분석 연구

김영호,고문석,제임스전,이영익,차정훈,현종건,김범수,임종만,이석인 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.6

This research aims at analysis of attack technique in sepaktakraw games. For this purpose, 3 high school coaches observed and recorded each game Five times in videos of high school male players' thirty games in 2003. The conclusion from the analysis of the recorded data are as follows. 1. Scissors spike was the most popular (favorite) attack technique. Rolling spike, tapping spike and heading spike was popular in order. 2. Heading spike showed the highest success rate (73.5%) in attack technique. And tapping spike (69.5%), scissors spike (57.8%) and rolling spike (67.11) showed a lower success rate in order. In failure rate, heading spike (5.5%), tapping spike (36.5%), scissors spike (32.2%) and rolling spike (32.9%) had a higher percentage in order. 3. When receive was stable, the success rate in attach technique was 47.1%, the failure rate was 20.1%. On the other hand, when receive was unstable, the success rate in attach technique was 16.5%, the failure rate was 14.3%.

解放後 濟州硏究槪觀(Ⅲ) : 社會科學分野

金榮敦,高南旭,夫萬根,申幸澈,金泰保,許鐵洙 제주대학교 탐라문화연구소 1985 탐라문화 Vol.0 No.4

실리콘 미세가공 기술을 이용한 마이크로 진공 센서

이광만,고성택,김영민,고희선 濟州大學校 工科大學 産業技術硏究所 1998 尖端技術硏究所論文集 Vol.9 No.1

A micro-vacuum sensor has been fabricated by using silicon micromachining techniques and platinum thin film. NON insulator was deposited by LPCVD and APCVD methods. The insulator diaphragm was fabricated by silicon anisotropic etching in TMAH solution. Thin film platinum heater and temperature detector were deposited by rf sputtering method and patterning was performed by means of lift-off method. Deposited platinum heater and temperature detector have been shown a linear Ⅰ-Ⅴ characteristics. TCR(temperature coefficient of resistance) of thin film platinum heater and temperature detector was about 0.0042/℃. Fabricated vacuum sensor was measured in the range of 1x10­³Torr to 1×10² Torr, and shows a good linear operating characteristics in the range of 1x10­² Torr에서 1x10˚Torr.