한국인 알코올의존환자에서의 제1형 부신피질자극 호르몬 분비 촉진 호르몬수용체의 유전자 다형성에 관한 연구

김철민,김성곤,김지훈,김현경,김미경,유정현,민은정 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

스트레스 반응에 중요한 역할을 하고 있는 CRHR1 유전자 SNP와 알코올 의존과의 연관성에 관한 선행 연구들이 있다. 이에 본 연구는 백인을 대상으로 조사했던 기존연구와는 달리 한국인 남녀를 대상으로 하여 알코올 의존군과 정상 대조군의 CRHR1 유전자형 빈도 및 대립유전자형 빈도를 비교하였다. 본 연구의 연구대상자들은 알코올 의존군 268명(남자 218명, 여자 50명)과 정상 대조군 137명(남자 78명, 여자 59명)이었다. CRHR1 유전자 SNP를 SNP database와 선행연구를 근거로 선정한 후, 연구 대상자들의 이러한 SNP 유전자형을 PCRRFLP방법으로 조사하였다. 그 결과, 한국인의 CRHR1 유전자 SNPs로 7개(rs242938, rs404623, rs28364027, rs16940686, rs937, rs878886, rs878887)가 분석되었다. 이중 rs28364027의 A 대립 유전자의 빈도는 남녀 각각에서 알코올 의존군이 대조군보다 유의하게 높았다. 그리고 rs28364027의 AA 유전자형 및 A 대립 유전자와 rs878886의 CC유전자형 및 C 대립유전자의 빈도는 알코올 의존군내에서 여성이 남성보다 유의하게 높았다. 이러한 결과를 종합해 보면 CRHR1 유전자 SNP가 알코올 의존의 유전적 원인의 중요한 요인일 가능성을 제시하고 있다. 또 이러한 CRHR1 유전자 SNP가 알코올 의존의 유전적 원인에서 남녀간 차이를 설명할 수 있을 가능성도 시사하고 있다. Objectives:There were several preceding studies investigating the association between alcohol dependence and corticotropine releasing hormone receptor1(CRHR1) gene SNPs that play an important role in response to stress. The frequencies of CRHR1 genotypes and alleles were compared between alcohol-dependent patients and normal control subjects. Methods:The subjects were 268 alcohol-dependent patients(218 males, 50 females) and 137 normal controls(78 male, 59 female). CRHR1 gene SNPs were investigated according to the SNP database and the results from previous studies, and their genotypes were analyzed by polymerase chain reaction(PCR) and restriction fragment length polymorphism(RFLP). Results:Seven CRHR1 gene SNPs(rs242938, rs404623, rs28364027, rs16940686, rs937, rs878886, rs878887) were found in Korean subjects. The frequency of rs28364027 A allele was significantly higher in alcohol-dependent patients than normal controls in both genders. In alcohol-dependent patients, the frequencies of AA genotype and A allele of rs28364027, and of CC genotype and C allele of rs878886 were higher in female than male. Conclusion:These results suggest that CRHR1 gene SNP is one of the important genetic factors in the etiology of alcohol dependence. And also it is supposed that the different frequency of SNP genotype could explain stress-related gender difference in the genetic etiology of alcohol dependence.

일반교육과 특수교육의 이중 프로그램에 대한 탐색

KangJong-Gu,Foley Dormot,KimJung Hyun 한국시각장애교육·재활학회 2008 시각장애연구 Vol.24 No.1

본 연구는 일반 교육과 특수교육을 동시에 배우는 복수전공 프로그램의 수업들을 가지는 두 명의 예비 교사들이 복수전공 프로그램과 통합교육에 대하여 어떻게 생각하는지에 대하여 탐구하였다. 연구자들은 두 명의 예비교사들이 통합교육 학급에서 장애로 명칭되어지는 학생들을 지원해 주고 싶어서 복수전공 프로그램을 다니기로 결정하였음을 발견하였다. 연구자들은 또한 두 명의 예비 교사들이 복수전공 프로그램의 수업들을 가지는 동안 장애와 통합교육에 대한 이해를 발전시켜 나가고 있음을 인식하였다. 이러한 발견들에 기초로 하여, 연구자들은 복수전공 프로그램이 한국의 교사 교육 프로그램들에 어떻게 적용되어질 수 있는지에 대하여 논의하고 있다.

Clinical efficacy of endoscopic ultrasonography for decision of treatment strategy of gastric cancer

Kim, Jung,Kim, Sang Gyun,Chung, Hyunsoo,Lim, Joo Hyun,Choi, Ji Min,Park, Jae Yong,Yang, Hyo-Joon,Han, Seung Jun,Oh, Sooyeon,Kim, Min Seong,Kim, Hyun Ju,Hong, Hyoungju,Lee, Hee Jong,Kim, Jue Lie,Lee, E Springer-Verlag 2018 Surgical endoscopy Vol.32 No.9

Chemical Modification of Intracellular Cytosine Deaminase from Chromobacterium violaceum YK 391

Kim, Jung,Kim, Tae-Hyun,Yu, Tae-Shick The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

Cytosine deaminase (cytosine aminohydrolase, EC 3.5.4.1) stoichiometrically catalyzes the hydrolytic deamination of cytosine and 5-fluorocytosine to uracil and 5-fluorouracil, respectively. Amino acid residues located in or near the active sites of the intracellular cytosine deaminase from chromobacterium violaceum YK 391 were identified by chemical modification studies. The enzymic activity was completely inhibited by chemical modifiers, such as 1mM NBS, chloramine-T, $\rho-CMB,\;\rho-HMB$ and iodine, and was strongly inhibited by 1mM PMSF and pyridoxal 5'-phosphate. This chemical deactivation of the enzymic activity was reversed by a high concentration of cytosine. Furthermore, the deactivation of the enzymic activity by $\rho-CMB$ was also reversed by 1mM cysteine-HCI, DTT and 2-mercaptoethanol. These results suggested that cysteine, tryptophan and methionine residues might be located in or near the active sites of the enzyme, while serine and lysine were indirectly involved in the enzymic activity. The intracellular cytosine deaminase from C violaceum YK 391 was assumed to be a thiol enzyme.

Osteoblastogenesis and osteoprotection enhanced by flavonolignan silibinin in osteoblasts and osteoclasts

Kim, Jung‐,Lye,Kang, Sang‐,Wook,Kang, Min‐,Kyung,Gong, Ju‐,Hyun,Lee, Eun‐,Sook,Han, Seoung Jun,Kang, Young‐,Hee Wiley Subscription Services, Inc., A Wiley Company 2012 Journal of cellular biochemistry Vol.113 No.1

<P><B>Abstract</B></P><P>Bone‐remodeling imbalance induced by decreased osteoblastogenesis and increased bone resorption is known to cause skeletal diseases such as osteoporosis. Silibinin is the major active constituent of silymarin, the mixture of flavonolignans extracted from blessed milk thistle (<I>Silybum marianum</I>). Numerous studies suggest that silibinin is a powerful antioxidant and has anti‐hepatotoxic properties and anti‐cancer effects against carcinoma cells. This study investigated that silibinin had bone‐forming and osteoprotective effects in in vitro cell systems of murine osteoblastic MC3T3‐E1 cells and RAW 264.7 murine macrophages. MC3T3‐E1 cells were incubated in osteogenic media in the presence of 1–20 µM silibinin up to 15 days. Silibinin accelerated cell proliferation and promoted matrix mineralization by enhancing bone nodule formation by calcium deposits. In addition, silibinin furthered the induction of osteoblastogenic biomarkers of alkaline phosphatase, collagen type 1, connective tissue growth factor, and bone morphogenetic protein‐2. Differentiated MC3T3‐E1 cells enhanced secretion of receptor activator of nuclear factor‐κB ligand (RANKL) essential for osteoclastogenesis, which was reversed by silibinin. On the other hand, RAW 264.7 cells were pre‐incubated with 1–20 µM silibinin for 5 days in the presence of RANKL. Non‐toxic silibinin markedly attenuated RANK transcription and intracellular adhesion molecule‐1 expression elevated by RANKL, thereby suppressing the differentiation of macrophages to multi‐nucleated osteoclasts. It was also found that silibinin retarded tartrate‐resistant acid phosphatase and cathepsin K induction and matrix metalloproteinase‐9 activity elevated by RANKL through disturbing TRAF6‐c‐Src signaling pathways. These results demonstrate that silibinin was a potential therapeutic agent promoting bone‐forming osteoblastogenesis and encumbering osteoclastic bone resorption. J. Cell. Biochem. 113: 247–259, 2012. © 2011 Wiley Periodicals, Inc.</P>

Report of the International Stem Cell Banking Initiative Workshop Activity: Current Hurdles and Progress in Seed‐Stock Banking of Human Pluripotent Stem Cells

Kim, Jung‐,Hyun,Kurtz, Andreas,Yuan, Bao‐,Zhu,Zeng, Fanyi,Lomax, Geoff,Loring, Jeanne F.,Crook, Jeremy,Ju, Ji Hyeon,Clarke, Laura,Inamdar, Maneesha S.,Pera, Martin,Firpo, Meri T.,Sheldon, John Wiley and Sons Inc. 2017 Stem cells translational medicine Vol.6 No.11

<P><B>Abstract</B></P><P>This article summarizes the recent activity of the International Stem Cell Banking Initiative (ISCBI) held at the California Institute for Regenerative Medicine (CIRM) in California (June 26, 2016) and the Korean National Institutes for Health in Korea (October 19–20, 2016). Through the workshops, ISCBI is endeavoring to support a new paradigm for human medicine using pluripotent stem cells (hPSC) for cell therapies. Priority considerations for ISCBI include ensuring the safety and efficacy of a final cell therapy product and quality assured source materials, such as stem cells and primary donor cells. To these ends, ISCBI aims to promote global harmonization on quality and safety control of stem cells for research and the development of starting materials for cell therapies, with regular workshops involving hPSC banking centers, biologists, and regulatory bodies. Here, we provide a brief overview of two such recent activities, with summaries of key issues raised. S<SMALL>TEM</SMALL> C<SMALL>ELLS</SMALL> T<SMALL>RANSLATIONAL</SMALL> M<SMALL>EDICINE</SMALL><I>2017;6:1956–1962</I></P>