Gene expression profiling of rat livers after continuous whole-body exposure to low-dose rate of gamma rays

Tran, Kim Ngan,Choi, Jong-il Informa Healthcare 2018 International Journal of Radiation Biology Vol.94 No.5

<P> Purpose: To study gene expression modulation in response to continuous whole-body exposure to low-dose-rate gamma radiation and improve our understanding of the mechanism of this impact at the molecular basis. Materials and methods: cDNA microarray method with complete pooling of samples was used to study expression changes in the transcriptome profile of livers from rats treated with prolonged lowdose-rate ionizing radiation (IR) relative to that of sham-irradiated rats. Results: Of the 209 genes that were two-fold-up or down-regulated, 143 were known genes of which 27 were found in previous literatures to be modulated by IR. Remarkably, there were a significant number of differentially expressed genes involved in hepatic lipid metabolism. Conclusion: This study showed changes in transcriptome profile of livers from low-dose irradiated rats when compared with that of sham-irradiated ones. This study will be useful for studying the metabolic changes of human exposed for long term to cosmic ray such as in space and in polar regions. </P>

High Yield Fermentation of L-serine in Recombinant Escherichia coli via Co-localization of SerB and EamA through Protein Scaffold

Kim-Ngan T. Tran,쿠마라벨 아쇽 쿠마,정재훈,홍순호 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.2

L-serine is a non-essential amino acid which has a wide range of applications and plays an important role as a building block for growing cells. L-serine microbial development is considered a difficult activity due to Lserine's central role in cellular metabolism with 2 main degradation pathways. A novel strategy is needed to overcome the L-serine degradation pathway and low Lserine tolerance of Escherichia coli for efficient L-serine production. A synthetic protein scaffold between SerB and EamA was introduced in this study to physically combine the two enzymes. Through this strategy, the L-serine production is more efficient than in competing pathways. By the introduction of a synthetic protein scaffold without metabolic pathway engineering or addition of glycine, 1.8 g/L of L-serine was produced at pH7 and 37°C. By fermentation, 9.4 g/L of serine was produced at a yield of 0.34 mol/mol glucose. These results suggest that the carbon flux was successfully directed to the L-serine secretion pathway without knocking out a competing pathway or adding expensive glycine.

Impact of the Synthetic Scaffold Strategy on the Metabolic Pathway Engineering

Kim-Ngan T. Tran,쿠마라벨 아쇽 쿠마,홍순호 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.3

For the development of the efficient bio-refinery process or biochemical producer, metabolic engineering has become an attractive choice recently. However, engineered metabolic pathways often suffer from flux imbalances due to a lack of corresponding regulatory mechanisms associated with natural metabolism. The interaction among different enzymes within a metabolic pathway plays an important role in regulating the efficiency of metabolic processes. Consequently, the creation of protein scaffolds has helped with the spatial co-localization of proteins in metabolic engineering. Research on protein scaffolds indicated scaffold systems may enhance metabolic productivity further. In this review, the specificity, selectivity, and regulatory mechanisms of protein-protein interactions are discussed in the context of the important effects that they exert on various biological processes.

Anti-neuroinfl ammatory effect of oxaline, isorhodoptilometrin, and 5-hydroxy-7-(2¢-hydroxypropyl)-2-methyl-chromone obtained from the marine fungal strain Penicillium oxalicum CLC-MF05

Dong-Cheol Kim,Tran Hong Quang,Nguyen Thuy Tien,Kwan-Woo Kim,Youn-Chul Kim,Nguyen Thi Thanh Ngan,Nguyen Xuan Cuong,Nguyen Hoai Nam,Hyuncheol Oh 대한약학회 2022 Archives of Pharmacal Research Vol.45 No.2

Penicillium is a rich source of bioactive compounds. Among all Penicillium species, Penicillium oxalicumhas been reported to produce various types of secondarymetabolites, including alkaloids, phenolics, and tetrahydroxanthonedimeric compounds, exhibiting many pharmacologicaleff ects, such as antiviral, antibacterial, and cytotoxicactivities. Three secondary metabolites were isolated from afermented culture of the sponge-associated fungal strain P. oxalicum CLC-MF05: oxaline ( 1 ), isorhodoptilometrin ( 2 ),and 5-hydroxy-7-(2′-hydroxypropyl)-2-methyl-chromone( 3 ). Their chemical structures were identifi ed by 1D and2D NMR and high-resolution mass spectroscopic analysesand compared with previously reported data. All three compoundsinhibited NO and PGE 2 overproduction and iNOSand COX-2 overexpression in both LPS-stimulated BV2and rat primary microglia. These metabolites also repressed mRNA expression of TNF-α, IL-1β, IL-6, and IL-12. Further,mechanistic studies revealed that the inhibitory actionsof compounds 1–3 were regulated by the inactivation of theNF-κB and MAPK signaling pathways. Furthermore, inactivationof the TLR4/MyD88 pathway contributed to theanti-neuroinfl ammatory activity of these compounds. Theseresults suggest that compounds 1–3 represent potential antiinflammatory candidates for the treatment of neurodegenerativediseases; however, further investigation is needed.

Brand Anthropomorphism, Brand Reputation, and Consumer Engagement in the Distribution of Smartphone Brands

Ngoc Dan Thanh NGUYEN(Ngoc Dan Thanh NGUYEN ),Trong Phuc NGO(Trong Phuc NGO ),Ngoc Van MAI(Ngoc Van MAI ),Kim Ngan TRA(Kim Ngan TRA ),Tran Huy Hoang LE(Tran Huy Hoang LE ) 한국유통과학회 2023 유통과학연구 Vol.21 No.4

Purpose: This study aims to analyze the impact of Brand Anthropomorphism and Intimacy on Brand Engagement, and at the same time analyze the regulatory effect of Brand Reputation on the relationship between Brand Anthropomorphism and Intimacy and the relationship between Intimacy and Brand Engagement in terms of distribution brand. Results: The findings show that Brand Anthropomorphism, Intimacy, and Brand Reputation are important value factors in customers’ minds toward their behavior, and from there, they will contribute to creating positive emotions and interactions between consumers and brands. Research design, data, and methodology: This article used the quantitative technique utilizing PLS-SEM software to test the hypothesis with 1,060 samples. Collected data shows that consumers in Ho Chi Minh City have positive emotions and interactive and social behaviors toward smartphone brands. Conclusion: The study has demonstrated the conclusions and proposed solutions to help smartphone brands build Brand Anthropomorphism while enhancing Brand Reputation thereby achieving Intimacy, which leads to consumer Brand Engagement. In addition, this study complements the concept of Brand Anthropomorphism which is lacking in theoretical background and is the first study in Vietnam to explore the prefixes and suffixes of the concept of Brand Anthropomorphism and the regulatory role of Brand Reputation.

Monitoring The Children’s Health Status and Forecasting Height with Nutritional Advice

Kim Ngan Nguyen,Nu Hoang Vi Ton,Tran Minh Khuong Vu,Pham The Bao 한국전기전자학회 2018 전기전자학회논문지 Vol.22 No.3

Children’s health is interesting to parents and society. A system that assists to monitor the development of their children and gives nutritional advices is an interesting of parents. In this study, we present a system that allows to track the heights and weights of a child since he/she was born up to adulthood, to predict his age of puberty, and to provide nutritional advice. Particularly, it predicts the height in near future and the adult stature for detecting the child with abnormal development. We applied Sager’s model for predicting the height in near future by using interpolation and regression techniques before puberty. After determining the puberty time, we proposed a model for predicting the height. Then we applied fuzzy logic for evaluating the health status and providing nutritional advice. Our system predicted stature in near future with error bound of 1.7361±0.0397 cm in girls and 2.4020±0.0799 cm in boys. Our model also gave a reliable adult stature prediction with error bound of 0.3507±0.2808 cm in girls and 1.3414±0.7024 cm in boys. At the same time, the nutrition was provided appropriately in terms of protein, lipid, glucid. We implemented a program based on this research. Our system promises to improve the health of every child.

Ni Nanoparticles Supported on MIL-101 as a Potential Catalyst for Urea Oxidation in Direct Urea Fuel Cells

Ngan Thao Quynh Tran,Hyo Sun Gil,Gautam Das,Bo Hyun Kim,Hyon Hee Yoon 한국화학공학회 2019 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.57 No.3

framework, MIL-101, particles. The morphology, structure, and composition of as synthesized Ni@MIL- 101 catalysts were characterized by X-Ray diffraction, Fourier transform infrared spectroscopy, scanning electron microscopy, and transmission electron microscopy. The electro-catalytic activity of the Ni@MIL-101catalysts towards urea oxidation was investigated using cyclic voltammetry. It was found that the structure of Ni@MIL-101 retained that of the parent MIL-101, featuring a high BET surface area of 916 m2 g-1, and thus excellent electro-catalytic activity for urea oxidation. A urea/H2O2 fuel cell with Ni@MIL-101 as anode material exhibited an excellent performance with maximum power density of 8.7 mWcm-2 with an open circuit voltage of 0.7 V. Thus, this work shows that the highly porous three-dimensional Ni@MIL-101 catalysts can be used for urea oxidation and as an efficient anode material for urea fuel cells.

α-Glucosidase Inhibitors from the Roots of Sophora flavescens

Quang, Tran Hong,Ngan, Nguyen Thi Thanh,Minh, Chau Van,Kiem, Phan Van,Tai, Bui Huu,Thao, Nguyen Phuong,Kwon, Se-Uk,Lee, Young-Mi,Kang, Hee-Kyoung,Kim, Young-Ho Korean Chemical Society 2012 Bulletin of the Korean Chemical Society Vol.33 No.5

Anti-inflammatory Triterpenoid Saponins from the Stem Bark of <i>Kalopanax pictus</i>

Quang, Tran H.,Ngan, Nguyen T. T.,Minh, Chau V.,Kiem, Phan V.,Nhiem, Nguyen X.,Tai, Bui H.,Thao, Nguyen P.,Tung, Nguyen H.,Song, Seok B.,Kim, Young H. American Chemical Society and American Society of 2011 Journal of natural products Vol.74 No.9

<P>Five new compounds, 16,23,29-trihydroxy-3-oxo-olean-12-en-28-oic acid (<B>1</B>), 4,23,29-trihydroxy-3,4-<I>seco</I>-olean-12-en-3-oate-28-oic acid (<B>2</B>), 3β,6β,23-trihydroxyolean-12-en-28-oic acid 28-<I>O</I>-β-<SMALL>d</SMALL>-glucopyranoside (<B>3</B>), 3-<I>O</I>-[2,3-di<I>-O</I>-acetyl-α-<SMALL>l</SMALL>-arabinopyranosyl]hederagenin 28-<I>O</I>-α-<SMALL>l</SMALL>-rhamnopyranosyl-(1→4)-β-<SMALL>d</SMALL>-glucopyranosyl-(1→6)-β-<SMALL>d</SMALL>-glucopyranoside (<B>4</B>), and 3-<I>O</I>-[3,4-di-<I>O</I>-acetyl-α-<SMALL>l</SMALL>-arabinopyranosyl]hederagenin 28-<I>O</I>-α-<SMALL>l</SMALL>-rhamnopyranosyl-(1→4)-β-<SMALL>d</SMALL>-glucopyranosyl-(1→6)-β-<SMALL>d</SMALL>-glucopyranoside (<B>5</B>), as well as 10 known compounds (<B>6</B>–<B>15</B>), were isolated from the stem bark of <I>Kalopanax pictus</I>. Compounds <B>1</B>–<B>5</B> and <B>7</B>–<B>14</B> inhibited TNFα-induced NF-κB transcriptional activity in HepG2 cells in a dose-dependent manner, with IC<SUB>50</SUB> values ranging from 0.6<B></B>to 16.4 μM. Furthermore, the transcriptional inhibitory function of these compounds was confirmed on the basis of decreases in COX-2 and iNOS gene expression in HepG2 cells. The structure–activity relationship of the compounds with respect to anti-inflammatory activity is also discussed.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jnprdf/2011/jnprdf.2011.74.issue-9/np200382s/production/images/medium/np-2011-00382s_0005.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np200382s'>ACS Electronic Supporting Info</A></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/np200382s'>ACS Electronic Supporting Info</A></P>

Monitoring The Children's Health Status and Forecasting Height with Nutritional Advice

Nguyen, Kim Ngan,Ton, Nu Hoang Vi,Vu, Tran Minh Khuong,Bao, Pham The Institute of Korean Electrical and Electronics Eng 2018 전기전자학회논문지 Vol.22 No.3

Children's health is interesting to parents and society. A system that assists to monitor the development of their children and gives nutritional advices is an interesting of parents. In this study, we present a system that allows to track the heights and weights of a child since he/she was born up to adulthood, to predict his age of puberty, and to provide nutritional advice. Particularly, it predicts the height in near future and the adult stature for detecting the child with abnormal development. We applied Sager's model for predicting the height in near future by using interpolation and regression techniques before puberty. After determining the puberty time, we proposed a model for predicting the height. Then we applied fuzzy logic for evaluating the health status and providing nutritional advice. Our system predicted stature in near future with error bound of $1.7361{\pm}0.0397cm$ in girls and $2.4020{\pm}0.0799cm$ in boys. Our model also gave a reliable adult stature prediction with error bound of $0.3507{\pm}0.2808cm$ in girls and $1.3414{\pm}0.7024cm$ in boys. At the same time, the nutrition was provided appropriately in terms of protein, lipid, glucid. We implemented a program based on this research. Our system promises to improve the health of every child.