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정명아,서유승,양진수,박준섭,윤진훈,이중건,이준승,이영규,김동희,조성범,주종은 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2
Pelvic actinomycosis is a chronic granulomatous suppurative disease caused by an anaerobic grampositive organism Actinomyces israelii. It is com-monly associated with an intrauterine device(IUD) and can mimick pelvic or intra-abdominal malignant neoplasm. Ureteral obstruction leading to hydronephrosis is a rare complication of tubo-ovarian abscess. We experienced a case of hydronephrosis as a complication of pelvic actinomycotic abscess. The patient was a 46-year-old women presenting with fever and right flank pain. Leukocytosis and pyuria were present and a hydronephrosis was diagnosed by intravenous pyelography. Ultrasonography and a computerised tomography revealed a mass in right adnexum compressing the right ureter. Removal of retroperitoneal abscess and salphingo-oophorectomy were done and the diagnosis of actinomycosis was made by pathologic finding of resected mass. Postoperatively, the patient was treated with second-generation cephalosporin successfully. (Korean J Nephrol 2002;21(2):337-340)
Kim, Wanhui,Kim, Hee-Eun,Lee, Ae-Ree,Jun, A Rim,Jung, Myeong Gyo,Ahn, Ji Hoon,Lee, Joon-Hwa Oxford University Press 2017 Nucleic acids research Vol.45 No.2
<P>MicroRNAs originate from primary transcripts containing hairpin structures. The levels of mature miR156 influence the leaf number prior to flowering in the life cycle of plants. To understand the molecular mechanism of biogenesis of primary miR156a (pri-miR156a) to mature miR156, a base-pair opening dynamics study was performed using model RNAs mimicking the cleavage site of wild type and B5 bulge-stabilizing mutant pri-miR156a constructs. We also determined the mature miR156 levels and measured leaf numbers at flowering of plants overexpressing the wild type and mutant constructs. Our results suggest that the stabilities and/or opening dynamics of the C15·G98 and U16·A97 base-pairs at the cleavage site are essential for formation of the active conformation and for efficient processing of pri-miR156a, and that mutations of the B5 bulge can modulate mature miR156 levels as well as miR156-driven leaf number phenotypes via changes in the base-pair stability of the cleavage site.</P>
Myeong Sook Cheon,윤태숙,Seung Ju Kim,Goya Choi,문병철,A-Yeong Lee,Byung Kil Choo,Ho Kyoung Kim,So Yeon Yu,Ken Yasukawa 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.2
The roots of both Aralia continentalis and Angelica biserrata, known as ‘Dokwhal’ in Korea, have been used widely as a traditional oriental medicine to treat inflammation and thrombosis. However, the pharmacological differences between A. continentalis and A. biserrata have not been fully established. In the present study, we investigated and compared the inhibitory effects of 70% ethanolic extracts of A. continentalis (ACE) and A. biserrata (ABE) on the production of inflammatory mediators and secondary swelling from chemically induced ear edema. In RAW264.7 macrophages, both ACE and ABE significantly inhibited the release of nitric oxide, prostaglandin E2, interlukin-1beta, and tumor necrosis factor-alpha in a dose dependent manner. In addition, the swelling from TPA-induced edema in mouse ears was reduced by ACE and ABE. Overall, ACE showed stronger activities than ABE in vitro and in vivo. Our results indicate that A. continentalis roots possess stronger anti-inflammatory activity than A. biserrata roots.
Cheon, Myeong-Sook,Yoon, Tae-Sook,Yasukawa, Ken,Yu, So-Yeon,Kim, Seung-Ju,Choi, Go-Ya,Moon, Byeong-Cheol,Lee, A-Yeong,Choo, Byung-Kil,Kim, Ho-Kyoung The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.2
The roots of both Aralia continentalis and Angelica biserrata, known as 'Dokwhal' in Korea, have been used widely as a traditional oriental medicine to treat inflammation and thrombosis. However, the pharmacological differences between A. continentalis and A. biserrata have not been fully established. In the present study, we investigated and compared the inhibitory effects of 70% ethanolic extracts of A. continentalis (ACE) and A. biserrata (ABE) on the production of inflammatory mediators and secondary swelling from chemically induced ear edema. In RAW264.7 macrophages, both ACE and ABE significantly inhibited the release of nitric oxide, prostaglandin $E_2$, interlukin-lbeta, and tumor necrosis factor-alpha in a dose dependent manner. In addition, the swelling from TPA-induced edema in mouse ears was reduced by ACE and ABE. Overall, ACE showed stronger activities than ABE in vitro and in vivo. Our results indicate that A. continentalis roots possess stronger anti-inflammatory activity than A. biserrata roots.
Litsenolide A2: The major anti-inflammatory activity compound in <i>Litsea japonica</i> fruit
Ham, Young-Min,Cho, Su-Hyeon,Song, Sang-Mok,Yoon, Seon-A,Lee, Yong-Bum,Kim, Chang-Sook,Kwon, Seung-Hae,Jeong, Myeong Seon,Yoon, Weon-Jong,Kim, Kil-Nam Elsevier 2017 Journal of Functional Foods Vol.39 No.-
<P><B>Abstract</B></P> <P>In this study, we investigated the anti-inflammatory effect of litsenolide A2 (LNA2) isolated from <I>L. japonica</I> fruit and its mechanism of action in LPS-induced RAW 264.7 cells. LPS-induced production of anti-inflammatory mediators and cytokines such as NO, PGE<SUB>2</SUB>, iNOS, COX-2, TNF-α, and IL-6 was significantly inhibited by LNA2. Next, western blot experiments were performed to investigate the mechanism of action of the anti-inflammatory effect of LNA2. The results indicated that LNA2 markedly reduced the LPS-induced activation of NF-κB and the nuclear translocation of NF-κB p65. Furthermore, LNA2 also inhibited the phosphorylation of MAPK by LPS. LNA2 was the major component in the ethanol extract of <I>L. japonica</I> at 59 mg/g according to high performance liquid chromatography (HPLC). These results indicate that LNA2 can be used as a functional and marker compound for standardization of the manufacturing process when <I>L. japonica</I> fruit is used as a functional food.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We study the anti-inflammatory effect of LNA2 in LPS-activated RAW 264.7 cells. </LI> <LI> LNA2 inhibited production of pro-inflammatory mediator and cytokines. </LI> <LI> LNA2 exhibited anti-inflammatory properties by suppression of NF-κB and MPAKs. </LI> <LI> LNA2 was the major component in the ethanol extract of <I>L. japonica</I> at 59 mg/g. </LI> </UL> </P>
( Jin Mi Chun ),( Myeong Sook Cheon ),( Byeong Cheol Moon ),( A Yeong Lee ),( Byung Kil Choo ),( Ho Kyoung Kim ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Although Asparagus cochinchinensis Merrill (Liliaceae) has traditionally been used for cancer treatment, its in vivo antitumor efficacy has not been established. Anti-tumor effect of the ethyl acetate fraction of A. cochinchinensis extract (EAF-ACE) was evaluated in nude mice xenografted with human hepatocellular carcinoma (HepG2) cells. Serum biomarkers were also examined to monitor the hepatotoxicity and nephrotoxicity of EAF-ACE. HepG2-xenografted nude mice were randomly divided into untreated group, EAF-ACE-treated groups (50, 100, or 200 mg/kg), and positive control group (3 mg/kg cisplatin). All groups were assayed for tumor mass formation, apoptosis induction, and serum biomarkers. EAF-ACE treatment significantly reduced tumor growth. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay revealed EAF-ACE treatment significantly increased the number of apoptotic cells in tumors in a dosedependent manner, whereas cisplatin treatment significantly decreased tumor volumes and increased the number of apoptotic cells, and exhibited hepatotoxicity and nephrotoxicity, in contrast to EAF-ACE treatment. EAF-ACE was found to possess antitumor activities without associated hepatotoxicity and nephrotoxicity. Therefore, EAF-ACE may be effectively used as a chemopreventive agent.
Anticancer Activity of Asparagus cochinchinensis Extract and Fractions in HepG2 Cells
( Mi Kyung Park ),( Myeong Sook Cheon ),( Seong Hwan Kim ),( Jin Mi Chun ),( A Yeong Lee ),( Byeong Cheol Moon ),( Tae Sook Yoon ),( Byung Kil Choo ),( Ho Kyoung Kim ) 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
Asparagus cochinchinensis Merrill (Liliaceae) has been traditionally used for the treatment of cancer in Korea and China, but its anticancer activity and underlying mechanism remain to be defined. Anticancer activities were investigated on fractions obtained from A. cochinchinensis 70% ethanol extract (ACE-EtOH) in human hepatocellular carcinoma HepG2 cells. Ethylacetate fraction from A. cochinchinensis extract (ACE-EA), more effective than other fractions, induced apoptosis of HepG2 (IC50=72.33±0.34 μg/mL), as revealed by apoptotic feature observation, increased capase-3 activity and Poly ADP ribose polymerase cleavage, and decreased expression of X-linked inhibitor of apoptosis protein in a dose-dependent manner. Protein levels of autophagy-related molecules, microtubule-associated protein 1 light chain 3 α and beclin 1, appeared to be induced by ACE-EA, suggesting ACE-EA exhibits anti-cancer activity with induction via both apoptosis and autophagy signaling pathways in HepG2 cells.
Anticancer Activity of Asparagus cochinchinensis Extract and Fractions in HepG2 Cells
Park, Mi-Kyung,Cheon, Myeong-Sook,Kim, Seong-Hwan,Chun, Jin-Mi,Lee, A-Yeong,Moon, Byeong-Cheol,Yoon, Tae-Sook,Choo, Byung-Kil,Kim, Ho-Kyoung The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
Asparagus cochinchinensis Merrill (Liliaceae) has been traditionally used for the treatment of cancer in Korea and China, but its anticancer activity and underlying mechanism remain to be defined. Anticancer activities were investigated on fractions obtained from A. cochinchinensis 70% ethanol extract (ACE-EtOH) in human hepatocellular carcinoma HepG2 cells. Ethylacetate fraction from A. cochinchinensis extract (ACE-EA), more effective than other fractions, induced apoptosis of HepG2 ($IC_{50}=72.33{\pm}0.34{\mu}g/mL$), as revealed by apoptotic feature observation, increased capase-3 activity and Poly ADP ribose polymerase cleavage, and decreased expression of X-linked inhibitor of apoptosis protein in a dose-dependent manner. Protein levels of autophagy-related molecules, microtubule-associated protein 1 light chain 3 ${\alpha}$ and beclin 1, appeared to be induced by ACE-EA, suggesting ACE-EA exhibits anti-cancer activity with induction via both apoptosis and autophagy signaling pathways in HepG2 cells.
Chun, Jin-Mi,Cheon, Myeong-Sook,Moon, Byeong-Cheol,Lee, A-Yeong,Choo, Byung-Kil,Kim, Ho-Kyoung The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.4
Although Asparagus cochinchinensis Merrill (Liliaceae) has traditionally been used for cancer treatment, its in vivo antitumor efficacy has not been established. Anti-tumor effect of the ethyl acetate fraction of A. cochinchinensis extract (EAF-ACE) was evaluated in nude mice xenografted with human hepatocellular carcinoma (HepG2) cells. Serum biomarkers were also examined to monitor the hepatotoxicity and nephrotoxicity of EAF-ACE. HepG2-xenografted nude mice were randomly divided into untreated group, EAF-ACE-treated groups (50, 100, or 200 mg/kg), and positive control group (3 mg/kg cisplatin). All groups were assayed for tumor mass formation, apoptosis induction, and serum biomarkers. EAF-ACE treatment significantly reduced tumor growth. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay revealed EAF-ACE treatment significantly increased the number of apoptotic cells in tumors in a dose-dependent manner, whereas cisplatin treatment significantly decreased tumor volumes and increased the number of apoptotic cells, and exhibited hepatotoxicity and nephrotoxicity, in contrast to EAF-ACE treatment. EAF-ACE was found to possess antitumor activities without associated hepatotoxicity and nephrotoxicity. Therefore, EAF-ACE may be effectively used as a chemopreventive agent.