HBx형질전환 생쥐에서 발생한 간세포암종에서 H-ras 및 c-myc의 발현에 관한 연구

문형배,소병준,김학철,윤기중,한원철,조향정,유대열,정영진 圓光大學校 醫科學硏究所 2002 圓光醫科學 Vol.17 No.2

<연구목적> HBx형질전환 생쥐에서 발생한 간세포암종의 발암과정에 종양유전자(H-rgs, c-myc)의 발현 정도를 조사하고자 하였다. <연구방법> 정상생쥐 12마리(4-18개월) 및 HBx 형질전환 생쥐 44마리(4-18개월)를 대상으로 포르말린에 고정하고 파라핀에 포매한 간 조직을 이용하여 면역조직화학적염색을 실시하였다. 실험군은 정상 부위, 이형성 부위 및 종양 부위로 구분하였으며, 종양 부위는 소결절성병변 부위와 간세 포암종 부위로 구분하였고, 이형성병변 부위는 이형성병변만 발견되는 부위, 소결절성병변과 동반된 이형성병변 부위 및 간세포암종과 동반된 이형성병변 부위로 구분하였다. <연구결과> H-rgs의 발현은 정상 간조직에 비하여 이형성병변 부위(P<0.05) 및 종양 부위(P<0.01)에서 증가하였으며, 소결절성병변 부위과 간세포암종 부위 사이에서는 간세포암종 부위에서 증가된 경향이었으나 통계학적으로 유의한 차이가 없었으며, 각 이형성병변 부위 사이에서도 유의한 차이는 없었다. c-myc의 발현은 정상 간조직 및 이형성병변 부위에 비해 종양 부위에서 증가하였으며(P<0.001), 소결절성병변 부위와 간세포암종 부위에서는 비슷하였고, 각 이형성병변부위 사이에서도 비슷하였다. <결론> HBx형질전환 생쥐에서 발생하는 간세포암종의 발생에 H-rgs는 이형성 변화를 일으키는 시기에 관여하며, c-myc은 이형성병변에서 암으로 이행하는 시기에 관여할 것으로 생각한다. Background: This experiment was designed for the expression of H-ras and c-myc in hepatocarcinogenesis of the HBx transgenic mice. Methods: Immunohistochemical stains in the paraffin embedded tissue of the liver were used for the detection of H-ras and c-myc in the 12 normal mice and 44 HBx transgenic mice of the 4-18 month old. Results: Expression of the H-ras was significantly increased in the dysplastic area (P<0.05) and tumor area (P<0.01) than in the normal liver. But there were no differences of H-ras expression between areas of microscopically identified hepatocellular carcinoma (MI-HCC) and grossly identified hepatocellular carcinoma (GI-HCC) and dysplastic areas among the only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Expression of the c-myc was significantly increased in the tumor area (P<0.001) than in the normal liver and dysplastic area. But there were no differences of c-myc expression between areas of MI-HCC and GI-HCC, and dysplastic areas among only dysplastic areas, dysplastic areas with MI-HCC and GI-HCC. Conclusions: Our study suggests that H-ras is related to the dysplastic change and c-myc is related to the neoplastic change in the hepatocarcinogenesis of the HBx transgenic mice.

Comparison of the Pharmacological Effects of Roasted and Non-roasted Zizyphi Spinosi Semen: Differences in Chemical Constituents

홍학기, 이기남, 마유안, 김윤배, 남상윤, 송인섭, 문동철, 오기완 충북대학교 동물의학연구소 2008 Journal of Biomedical and Translational Research Vol.9 No.3

본문참고

중소형 화학공장을 위한 다단 증류 분리 장치의 통합설계 Package 개발 연구

정재학,이기홍,곽철규,이문용 嶺南大學校 工業技術硏究所 2001 工業技術硏究所論文集 Vol.29 No.2

The purpose of this research is to develop the GUI environmental software package for efficient analysis of multi-component distillation column simulation. It should give the expediency of interpretation of complex data and saving time for generating the design of column by graphic display data analysis tool. Generating a column design result with conventional software package needs many trial-and-error simulation with consuming time works but the result of this research gives the optimal simulation result with just single graphic view. Furthermore, the developed package contains tools for dealing with various data and solving modules for several complex distillation column problems. The routines of physical property data included bubble-point, dew-point, density, entropy are also developed.

축하중과 첨가질량이 작용하는 적층복합판의 진동해석

이봉학,김경진,원치문,성기태 江原大學校 産業技術硏究所 1995 産業技術硏究 Vol.15 No.-

A method of calculating the natural frequency corresponding to the first mode of vibration of beams and tower structures, with irregular cross-sections and with arbitrary boundary conditions was developed and reported by D. H. Kim in 1974. IN this paper, hte result of application of this method to the laminated plates with axial forces and with attached point mass/masses is presented. Both N? and N? forces are considered. The solution for the laminated plates with arbitrary boundary conditions and irregular section can be obtained by simply obtaining the deflection influence coefficients by any method. The effect of neglecting the mass of the plates on the natural frequency, as the ratio of the point mass/masses to the plate mass increases, is thoroughly studied. The influence of N? and N? is also carefully investigated.

TiAIN 코팅 평 엔드밀의 성능평가에 관한 기초 연구

유중학,국정한,김문기 한국공작기계학회 2001 한국생산제조학회지 Vol.10 No.5

The purpose of this study is an evaluation of flat end mills to develop appropriate tools for the high speed machining. First of all, several flat end mills which are produced by different markers are selected to analyze the performances of the tools. Experimental works are also executed to measure cutting force, tool wear and surface roughness for different cutting conditions. And then the results are compared and analyzed for developing optimal cutting tool in the high speed condition.

비만과 전립선암의 연관성 분석

윤창준,문기학,박동춘 영남대학교 의과대학 2005 Yeungnam University Journal of Medicine Vol.22 No.2

Background: The role of obesity in prostate cancer etiology remains controversial. The aim of this study was to evaluate the relationship between obesity and prostate caner risk Material and methods: Between January 2000 and June 2005, 286 patients suspected of having prostate cancer underwent prostate biopsy. The clinical records of the 286 study patients were retrospectively reviewed with regard to age Body Mass Index (BMI), serum PSA, TRUS, and prostate biopsy results. They were stratified by BMI into three groups according to the cutoffs recommended for Asian population: normal. BMI less than 23 Kg/m^(2), overweight , BMI 23 to 25 Kg/m^(2), and obese, BMI greater than 25 Kg/m^(2). Results: As for BMI, 132 (46.2%) were normal, 95 (33.2%) overweight and 59 (20.6%) were obese. A total of 99 (34.6%) patients were diagnosed as having prostate cancer. In multivariate logistic regression analyses, no significant association was observed between BMI and prostate cancer detection. Conclusion: We initially hypothesized that obesity may be biologically associated with increased prostate cancer development. However, our study did not show a significant association between BMI prostate cancer.

저작근 골막하 박리를 이용한 만성 재발성 탈구의 치험례

심재환,김기홍,문진석,민병진,이원학,홍광진 大韓顎顔面成形再建外科學會 2003 Maxillofacial Plastic Reconstructive Surgery Vol.25 No.3

Chronic recurrent dislocation(CRD) of the jaw has various etilogical causes, including trauma, congenital or acquired neurological illness, ingestion of certain medicines, incorrdination of the neuromuscular astivity of the chewing muscles, articular anatomical alteration, etc. Various surgical methods have been advocated for the treatment of the CRD. They may be divided into two groups: procedures designed to limit translation of the condyle and procedures eliminating obstacles in the condylar path. Hypomobility of mandible can be developed after BSSRO and it is probably caused by scar contracture, atrophy, and myofibrosis. Many authors, therefore, emphasized the importance of physical therapy especially after BSSRO to prevent the complication. Based on that idea, we designed more conservative surgical method to limit mandibular openning compared the methods that previously introduced and tried to treat the CRD. This new method consists of extensive subperiosteal dissection of masseter and the temporalis muscle under local anesthesia and use of intermaxillary elastics for some periods.

Efficacy of Once-Daily Administration of Udenafil for 24 Weeks on Erectile Dysfunction: Results from a Randomized Multicenter Placebo-Controlled Clinical Trial

Moon, Ki Hak,Ko, Young Hwii,Kim, Sae Woong,Moon, Du Geon,Kim, Je Jong,Park, Nam Cheol,Lee, Sung Won,Paick, Jae-Seung,Ahn, Tae Young,Chung, Woo Sik,Min, Kwon Sik,Park, Jong Kwan,Yang, Dae Yul,Park, Kwa Elsevier 2015 JOURNAL OF SEXUAL MEDICINE Vol.12 No.5

<P>The method of administration of oral phosphodiesterase-5 inhibitors has been expanded to once-daily repeated administration with lower initial dosage than on-demand administration.</P>

Obesity and Erectile Dysfunction: From Bench to Clinical Implication

Ki Hak Moon,So Young Park,Yong Woon Kim 대한남성과학회 2019 The World Journal of Men's Health Vol.37 No.2

Obesity is a major public health issue worldwide and is frequently associated with erectile dysfunction (ED). Both condi-tions may share an internal pathologic environment, also known as common soil. Their main pathophysiologic processes are oxidative stress, inflammation, and resultant insulin and leptin resistance. Moreover, the severity of ED is correlated with comorbid medical conditions, including obesity. Therefore, amelioration of these comorbidities may increase the efficacy of ED treatment with phosphodiesterase 5 inhibitors, the first-line medication for patients with ED. Although metformin was originally developed as an insulin sensitizer six decades ago, it has also been shown to improve leptin resistance. In addition, metformin has been reported to reduce oxidative stress, inflammatory response, and body weight, as well as improve ED, in animal and human studies. Moreover, administration of a combination of metformin and phosphodiesterase 5 inhibitors improves erectile function in patients with ED who have a poor response to sildenafil and are insulin resistant. Thus, con-comitant treatment of metabolic derangements associated with obesity in patients with ED who are obese would improve the efficacy and reduce the refractory response to penile vasodilators. In this review, we discuss the connecting factors between obesity and ED and the possible combined treatment modalities.

제조방법이 다른 떡차의 저장기간에 따른 성분학적 특성

강진영(Jin Young Kang),조정용(Jeong Yong Cho),김선재(Seon Jae Kim),신기호(Ki Ho Shin),김두운(Du woon Kim),박기영(Ki Young Park),박근형(Keun Hyung Park),문제학(Jae Hak Moon) 한국차학회 2014 한국차학회지 Vol.20 No.4

The chemical constituents in ddeok chas produced by three different manufacturing procedures were analyzed to determine their characteristics. Sample-1 was prepared by steaming, pounding, shaping, drying, and stored indoors. Sample-2 was prepared by pounding fresh tea leaves, shaping, steaming, drying, and stored indoors. Sample-3 was prepared by withering, rolling (rubbing), steaming, pounding, shaping, drying, and stored indoors. These samples were stored under the same conditions and the total catechin, total theaflavin, gallic acid, caffeine, and ascorbic acid contents in each sample were analyzed by HPLC. With the exception of ascorbic acid, the levels of the other compounds decreased with the increasing of storage period. The chemical constituents showed a similar pattern regardless of the ddeok cha type, despite being stored for 1 year. This suggested that the manufacturing procedures are an important factor for the quality of ddeok cha. In addition, the storage method also affects the antioxidative activity of ddeok cha.