Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1

Sohn, Sea-Hwan,Cho, Eun-Jung,Jang, In-Surk The Korean Society of Animal Reproduction 2006 Reproductive & developmental biology Vol.30 No.4

The Chinese Hamster Ovarian cells CHO-K1 are one of the most extensively used cells for the evaluation of gene expression and toxicology. However, these cells are frequently used for biomedical research without consideration of their cytogenetic characteristics. Therefore, we carried out to investigate the karyologic profiles, the frequency and type of chromosome aberration, and the distribution of telomeric DNA on chromosomes of the CHO-K1 cells. The GTG banding and fluorescence in situ hybridization on CHO-K1 cells were performed to characterize the karyotype and the distribution of telomeric DNA The present study revealed that the chromosome modal number of CHO-K1 cells was 2n=20; eight chromosomes appeared to be identical with those of the normal Chinese hamster, whereas the remaining 12 chromosomes were shown to be translocated, deleted, inversed, or rearranged from Chinese hamster chromosomes. The telomeric DNA on CHO-K1 chromosomes was intensively distributed at the centromeres rather than the ends of chromosomes. In addition, three chromosomes had interstitial telomeres and one marker chromosome entirely consisted of telomeric DNAs. The frequency and type of chromosome aberrations in CHO-K1 cells were examined. Of the 822 metaphase spreads, 68 (8.3%) cells resulted in chromosome aberrations of which the chromosome breakage was the most frequently occurred.

연구논문 : 발효차 청태전 제조용 미생물의 분리

박정숙 ( Jung Suk Park ),조정일 ( Jung Il Cho ) 한국식생활문화학회 2011 韓國食生活文化學會誌 Vol.26 No.2

Chungtaejeon is a traditional tea introduced in the age of the Three States and is the only “Don-cha” culture in the world that survived on the southwestern shore of Korea. To restore Chungtaejeon and to make the tea with consistent quality, the microorganisms involved in traditional type fermentation of Chungtaejeon were isolated, and the tea was prepared with high fermentation ability starters. The sensuous characteristics of Chungtaejeon were also examined. Only Bacilli were found in 3 and 5 year aged Chungtaejeon samples. The Lactobacilli were isolated from properly fermented kimchi and one of them showed high growth capability in media containing green tea extract and also showed strong antagonistic activity against methicillin-resistant Staphylococcus aureus, S. aureus, Salmonella, and E. coli. It was identified and named Lactobacillus plantarum CHO25. Chungtaejeon was fermented with a single starter of L. plantarum CHO25 and with a mixed starter (L. plantarum CHO25, Saccharomyces cerevisiae and Bacillus amyloliquefaciens CHO104). The single fermented sample had the highest cell growth after 5 days of inoculation and the level decreased slowly thereafter. The mixed fermented sample showed strong growth of S. cerevisiae. The highest hunter values were the a value of the single fermented sample and the b value of the mixed sample. The single fermented tea showed the best incense score.

消積白朮散의 癌 轉移 抑制와 T 및 B 임파구의 증식에 미치는 영향

Jung, Chel-Jong,Son, Chang-Kyu,Cho, Chong-Kwan 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Chel-Jong Jung, Chang-Kyu Son, Chong-Kwan Cho. Department of Oriental Internal Medicine, Oriental Medical Hospital, Taejon University, Taejon, Korea. Studies on the effects of sojekbaekchoolsan on immune response & metstasis of B16-Fo melanoma cells. Proceedings of International Symposium on East-West Medicine, Seoul. 117-127, 1999.-This study was carried out to investigate the effect of Sojeokbaekchoolsan on immune response and metastasis of B16-Fo melanoma cells. The result were summarized as follows:1. Metastasisi of B16-Fo melanoma cell in C57BL/6 mice were decreased in the Sojeokbaekchoolsan treated group as compared with the control group. 2 Blastic response of lymphocyte to Con A were increased in the Sojeokbaekchoolsan teated group as compared with the control group. 3. Blastic response of lymphocyte to LPS were increased in the Sojeokbaekchoolsan treated group as compared with the control group. 4 Blastic response of lymphocyte to PWM were increased in the Sojeokbaekchoolasn treated group as compared with the control groupl From the above results, It is suggested that Sojeokbaekchoolsan enhanced as well cellula-mediated and humoral immune response as sojeokbaekchoolsan supressed the metastasis of tumor cells as compared with the control group.

Development of A Teledermatological Service System Among Korea-China-Europe Using TEIN

Cho, Jongman,Jung, j Y,Kim, S W 仁濟大學校 2006 仁濟論叢 Vol.21 No.1

A teledermatological service system among Korea, China and Austria has been developed and tested in this research. For successful design and establishment of a teledermatological service system, four elements were considered-networking among hospitals and participants, standard specification for teledermatological consultation, database for medical record, and design and implementation of software. In addition to the fundamental stationary system, mobile teledermatological service function using PDA was added to provide dermatologists with ubiquitous consultation service. It was evaluated and proved to be a feasible for tele-consultation system by the dermatologists participated in this research.

치매방지 작업기구 사용이 항산화효소 활성에 미치는 영향

조경혜,박정은,윤지윤,문세보,박효영,정인옥,이정원 서울여자대학교 자연과학연구소 2001 자연과학연구논문집 Vol.13 No.-

We developed an occupational therapy tool to maintain and improve the cognitive function for the elderly who have senile dementia. 86 elderly people took a mininal mental state examination (MMSE) for the therapy to perform the occupational therapy, and divided into three groups based on avarage ages and MMSE values. The occupational therapy was treated three times per week for 10 weeks. The results showed that there were significant differences in the performing scores before and after the treatment ; the scores for the occupational therapy were increased significantly (p<0.001) and the performing time was shortened from 6-week throughout 10-week regimen. This study was to examine the effect on the activity of antioxidant enzymes by occupational therapy. The activities of antioxidant enzymes such as catalase, Glutathion-6-phosphate dehydrogenase (G6PDH), and superoxide dismutase (SOD) were increased to the normal standard by the occupational therapy for the elderly having dementia. The recovery of activities of antioxidant enzymes was obvious in severe demented elderly compared to those in normal or mild demented elderly. These results suggest that the occupational therapy had an beneficial effect on the protection against the oxidative stress especially for severe demented elderly, and the occupational tool need to be modified so as to improve the protection against the oxidative stress for normal and/or mild demented elderly.

Cytogenetic Characteristics of Chinese Hamster Ovarian Cell CHO-K1

Sea Hwan Sohn,Eun Jung Cho,In Surk Jang 한국동물생명공학회(구 한국동물번식학회) 2006 Reproductive & developmental biology Vol.30 No.4

The Chinese Hamster Ovarian cells CHOK1 are one of the most extensively used cells for the evaluation of gene expression and toxicology. However, these cells are frequently used for biomedical research without consideration of their cytogenetic characteristics. Therefore, we carried out to investigate the karyologic profiles, the frequency and type of chromosome aberration, and the distribution of telomeric DNA on chromosomes of the CHOK1 cells. The GTGbanding and fluorescence in situ hybridization on CHOK1 cells were performed to characterize the karyotype and the distribution of telomeric DNA. The present study revealed that the chromosome modal number of CHOK1 cells was 2n=20; eight chromosomes appeared to be identical with those of the normal Chinese hamster, whereas the remaining 12 chromosomes were shown to be translocated, deleted, inversed, or rearranged from Chinese hamster chromosomes. The telomeric DNA on CHOK1 chromosomes was intensively distributed at the centromeres rather than the ends of chromosomes. In addition, three chromosomes had interstitial telomeres and one marker chromosome entirely consisted of telomeric DNAs. The frequency and type of chromosome aberrations in CHOK1 cells were examined. Of the 822 metaphase spreads, 68 (8.3%) cells resulted in chromosome aberrations of which the chromosome breakage was the most frequently occurred.

Periapical multilocular osteoporotic bone marrow defect

Jung, Yun-Hoa,Cho, Bong-Hae,Nah, Kyung-Soo 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.4

A case of osteoporotic bone marrow defect, which appeared as a well-defined multilocular radiolucency overlapping the roots of mandibular right second molar, was reported. On periapical radiograph, a daughter cyst-like radiolucency was seen at the anterior margin of the lesion making it difficult to rule out odontogenic keratocyst.

熱處理 條件에 따른 PET 織物의 減量加工에 關한 硏究

배정숙,조환,허정순,조용석 대구대학교 산업기술연구소 1985 産業技術硏究 Vol.4 No.-

This study has been undertaken to see the variation of the weight loss, dyeing property, and the tensile strength of the polyethylene terephthalate (PET) fabrics under the influence of the heat setting during the finishing of the fabrics. The fabrics were heat set for 40,80 and 120 seconds, then alkali treated with 5%, caustic soda solution for 40 minutes on temperature of 80℃, and then dyed with blue and red disperse for 30 minutes of the temperature of 130℃, Using these treated materials, color differences and tensile strength were measured. The results were as follows; 1. The weight loss of PET fabrics increased as the heat setting temperature was elevated. 2. weight loss of PET fabrics decreased as heat setting time increased on the temperature over 220℃ 3. The dyeing property of PET fabrics became stable on the temperature below 180℃ not influenced by heat setting time. 4. The tensile strength decreased as the weight loss increased. Consequently, the temperature influences greatly on the weight loss and the dyeing property during the finishing of PET fabrics.

尿血과 IgA 腎症에 대한 比較 考察

李政遠,趙忠植,金哲中 대전대학교 한방병원 2000 惠和醫學 Vol.9 No.1

According to the comparative study on the IgA nephropathy and hematuria, the results were as follows. 1. IgA nephropathy seems to be caused by deposits of IgA antibodies in the kidney mesangial tissues. 2. The symptoms of IgA nephropathy are repetitive hematuria, secondarily asthenia, slight fever, the urination pain and the flank pain etc. 3. Steroids have a few effect on the incipient stage of IgA nephropathy, but the specific medicine isn't in existence at present. 4. In oriental medicine, hematuria defines the urine including blood or blood clot without pain in urination. 7. The causes of hematuria are cases of heat and asthenia of kidney(腎虛). 8. For the medical prescriptions are used Paljungsan(八正散), Dojo˘ksan(導赤散), Yo˘ngyopaedoksan(連翹敗毒散), Singihwan(腎氣丸), sogyeeumja(小계飮子), Daebuncho˘ngeum(大分淸飮), Dangguieum(當歸飮), Cho˘ngjangtang(淸腸湯), Silyungtang(柴영湯), Kamisagunjatang(加味四君子湯), Jwaguieum(左歸飮)

한국전쟁과 지방화단

조은정(Cho Eun-Jung) 부산대학교 한국민족문화연구소 2010 한국민족문화 Vol.38 No.-

1950년에 발발한 한국전쟁은 식민지에서 벗어난 지 얼마 되지 않은 상태였던 한국 사회의 많은 것을 변화시켰고 미술인의 활동에도 일정 부분 변화를 가져왔다. 월북과 월남 그리고 부산으로의 피난으로 대표되는 작품활동 지역의 변화는 근거지를 잃은 작가들이 고향과 경제력을 상실하게 함으로써 개인적인 패배감을 강화시켰을 뿐만 아니라 심리적인 공황까지 야기시켜 작가 자신의 세계를 개척하기 어렵게 만들었다. 하지만 전쟁 중에도 화가들은 다방에서 개인전이나 그룹전을 열어 창작열을 불태웠으며 대한미술협회라는 단체를 결성하여 전열을 정비하기도 하였다. 그림 그릴 재료가 곤궁하고 생활이 어려운 상황에서도 미술에 열중할 수 있다는 프로의식과 예술에의 의지를 볼 수 있는 이들 전시문화에는 당시의 상황에 대응한 작가의 현실 인식이 반영되어 있다. 현실 도피로서의 순수에의 집념, 무능한 가장으로서 현실적으로 해낼 수 있는 유일한 일인 그림그리기, 그 어느 것이든 한국전쟁기 화단의 활동은 멈추지 않았는데 이는 상당히 중요하며 고무적인 일이다. 다만 이념의 갈등이 빚어낸 전쟁이고 자신의 국토에서 발발한 전쟁이지만 정작 자신들은 전쟁의 주체가 되지 못한 전쟁이 한국전쟁이었다는 뼈아픈 사실을 감안하지 않으면 안 된다. 전쟁이 건국초기에 비하여 한층 더 강화된 반공이념을 정당화시키는 상황에서 미술단체와 미술제도 그리고 개개인은 이념으로부터 자유로울 수 없었다. 이념적 성향에 따라 세속적 성공과 좌절이 결정되고 마는 한계 상황에 설 수 밖에 없게 되었던 것이다. 인적 구성의 변화는 남한 내에서도 마산에 있던 임호나 이준이 부산으로 왔고, 또 휴전과 함께 이준은 서울에서 열린 제2회 〈국전〉에 화려하게 등장하였다. 미술인의 이동은 월남이나 월북 이외에 남한 내에서도 지방에의 정착과 지방미술인의 상경으로 이루어졌다. 피난민으로서 미술가들의 위상은 거주지를 옮겼음에도 끊임없이 전시를 하거나 미술작품의 창작을 쉬지 않음으로써 미술가로서의 정체성을 유지한 것이었다. 일찍이 미술에서 그렇게 활발하게 직접적으로 중앙과 지방의 교류가 이루어졌던 적은 없었다. 미술인들은 자신이 거주하는 곳의 환경과 의미를 느꼈고 그것을 어떠한 방식이든 작품에 투사하였다. 박고석의 부산의 여러 장소에 대한 풍경이나 이중섭의 제주도 풍경이 그러한 예이다. 또한 부산에 거주하는 작가들에게 다른 지역 작가들 특히 서울에서 피난온 모더니즘 계열의 자극은 자신이 속한 지역의 색채에 대한 깊이있는 논의로 이어져 전통에 대한 관심과 민족적인 미술의 확산으로 이어졌던 것도 주목할 일이다. 지방과 중앙 혹은 피난민 화가들과 지역작가들과의 관계는 일방적 영향관계로 파악했을 때, 상대에 대한 부정으로 작용하여 문화적 교류 혹은 충격이라는 사실조차 부정하게 되는 것을 본다. 그리고 당시에조차 그러한 시각은 중앙의 모더니즘 대 지방의 전통이라는 이원적인 시각을 양산하는 배경이 되었다. The Korean War that broke out in 1950 had brought a lot of changes into the Korean society, as it was only a while after becoming decolonized. It also had affected certain parts of artists’ activities. Due to evacuating toward north or south over the border, and to Busan, artists loosing their hometown and economic strength were the changes in major regions of creating pieces. Therefore, it didn’t only enhancethe sense of defeat, but also caused psychological panic so it made the artists even more difficult to develop their own world. However, artists opened private or group exhibitions in coffee shops to express their creativity. They also formed a group called ‘Korean Association of Art’ and settled the combative fervor. Artists’culture of exhibitions reflected their cognitive reality of coping with the circumstances during that time and it proved that even with scarce art materialsand difficult living environment, they could concentrate on art as professionals and it represented their will of art. Being deeply attached to purity to avoid the reality, as being an incompetence head of a household, the only thing they would do was drawing; however, none of the reasons stopped them from participating in the world of artists during the Korean War, which was really important and encouraging. Even Korean War was aroused in Korea, Korea wasnot the main subject of the war, and it started due to ideological conflicts. Therefore, the fact that Korean War was a definite agony should not be taken out of consideration. It was not possible for artists’organization, art institutions, or individuals to express their ideology fully after the war, since the anti-communism sentiment had become even more justified, compared to initial stage of establishing a country. They had to face the limited reality where secular success and breakdown were decided by their ideological propensity. The change in formation of human footsteps had also existed in South Korea; Lim, Ho and Lee, Jun had movedto Busan from Masan, and as armistice started, Lee, Jun had remarkably exposed himself through 2n the National Art Exhibition in Seoul. The movement of artists was not only crossing over the border to South or North Korea, but some artists settled in rural regions, and some came up to Seoul from rural areas. Even changing their place of residence as refugees, artists maintained their identity by opening art exhibitions constantly and not giving up on creating artworks. There never had been that direct and active way of interchange in art between the rural and central regions. Artists were impressed by their surrounding environments and significances, thus they portrayed it in their artworks in anyway. Several scenery of Busan by Park, Go-suk, and Jeju Island’s scenery by Lee, Jung-Sup are the examples. Also, the artists of modernism line from other regions’, especially Seoul, had stimulated artists living in Busan. It should be noted that, the fact of having in-depth discussion about their regional coloration among the artists had led them to become more interested in the tradition, and it even extended the field of art. By understanding the relations between the rural and central, the refugee artists and regional artists, respectively, were unilaterally influential relations they even disagreed on the fact that it was cultural exchange or cultural shock since they impactedeach other negatively. Even during that time, that kind of viewpoint had become the background of setting the ‘central modernism vs. rural tradition’ dualistic perception.