Improved Micropropagation of Root Chicory, Cichorium intybus L. var. sativus.

Lim,Jung-Dae,Yang,Deok-Chun,Lee,Hyeon-Yong,Kim,Jong-Dai,Lee,Jin-Ha,Sung,Eun-Soo,Yu Chang-Yeon 한국자원식물학회 2004 Plant Resources Vol.7 No.1

The establishment of an efficient protocol for plant regeneration and micropropagation from leaf explant cultures of Chicory, Cichorium intybus L. var. sativus. is reported. Callus formation rate appeared 100% from explant in all growth regulators, but calli formed in the prensence of naphthaleneacetic acid (NAA) were appeared very compact and non-embryogenic state. The regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The highest number of shoots (5.7) per explant and shoot growth (2.8cm) was obtained on MS medium containing 0.1 mg BAP L^-1 and 0.1 mg NAA L^-1. Indole acetic acid was the most suitable auxin for root formation among three auxins tested. 2,4-D had no effect on shoot and root formation.

THE DEVELOPMENT OF A UV FLAME DETECTOR FOR THE AUTOMATIC FIRE SUPPRESSION SYSTEM FOR ENGINE COMPARTMENT FIRES

Lim,Sung-Mook,Jung,Ki-Chang,Kim,Eung-Sik,Kim,Hong 한국화재소방학회 1997 한국화재소방학회 학술대회 논문집 Vol.1997 No.-

In this work, a new type of UV flame detection system was developed. In order to measure the performance of UV flame detector, various kinds of experiments was performed. The results show that the maximum response time of the UV flame detector is 0.2 seconds when the detection distance is one meter The advantages of this system include wide area, high speed response and high sensitivity. After testing the W flame detector in engine compartment it detected fire within 0.09 seconds and extinguished within 5 seconds. Hence, the UV flame detector can be applied in automatic fire suppression system for automobiles.

Identification of Novel Endo-β-1,4-glucanase Isoforms fromBursaphelenchus Species (Nemtoda: Aphelenchoididae)

Dae-Weon Lee,Il Sung Moon,강재순,Hyun Jung Choe,최재영,제연호,이시혁,Hye Rim Han,Kook-Jin Lim6,Young Ho Koh 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.2

Bursaphelenchus xylophilus has been recognized as a causal pathogen of pine wilt disease (PWD). In order to identify relevant molecular biomarkers, we selected a variable region of endo-β-1,4-glucanase (β14-gcn) which was cloned from the cDNA of B. xylophilus and B. mucronatus. We then identified three novel β14-gcn isoforms: Bm-β14-gcn and Bm-β14-gcni from B. mucronatus, and Bx-β14-gcn from B. xylophilus. Bm-β14-gcn and Bx-β14-gcn were found to be identical, whereas Bm-β14-gcni was unique to B. mucronatus. These isoforms are Bursaphelenchus-specific and may be employed as molecular markers for the diagnosis of PWD. Our phylogenetic analysis showed that these β14-gcns from Bursaphelenchus species were associated most closely with fungal β14-gcns.

Interaction of <i>Veratrum nigrum</i> with <i>Panax ginseng</i> against Obesity: A Sang-ban Relationship

Park, Jinbong,Jeon, Yong-Deok,Kim, Hye-Lin,Lim, Hara,Jung, Yunu,Youn, Dong-Hyun,Jeong, Mi-Young,Kim, Hyun-Ju,Kim, Sung-Hoon,Kim, Su-Jin,Hong, Seung-Heon,Um, Jae-Young Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-

<P>Obesity has become a major health threat in developed countries. However, current medications for obesity are limited because of their adverse effects. Interest in natural products for the treatment of obesity is thus rapidly growing. Korean Medicine (KM) is characterized by the wide use of herbal formulas. However, the combination rule of herbal formulas in KM lacks experimental evidence. According to <I>Shennong's Classic of Materia Medica</I>, the earliest book of herbal medicine, <I>Veratrum nigrum</I> (VN) has antagonistic features against <I>Panax ginseng</I> (PG), and the PG-VN pair is strictly forbidden. In this study, we have shown the effects of PG, VN, and their combination on obesity in high-fat (HF) diet-induced obese mice and in 3T3-L1 cells. PG, VN, and PG-VN combination significantly reduced weight gain and the fat pad weight in HF diet-induced obese mice. They also significantly decreased lipid accumulation and the expressions of two major adipogenesis factors, PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I>, in 3T3-L1 cells. In addition, the PG-VN combination had synergistic effects compared with the mixture of extracts of PG and VN on inhibition of PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I> expressions at lower doses. These results indicate a new potential anti-obese pharmacotherapy and also provide scientific evidence supporting the usage of herbal combinations instead of mixtures in KM.</P>

Risk Factors for Decompression Retinopathy After Glaucoma Surgery

Jung, Kyoung In,Lim, Sung A.,Lopilly Park, Hae-Young,Park, Chan Kee by Lippincott Williams Wilkins. 2014 Journal of glaucoma Vol.23 No.9

PURPOSE:: To evaluate the risk factors for decompression retinopathy after glaucoma surgery. METHODS:: We reviewed the medical records of patients who underwent a trabeculectomy or Ahmed valve implantation and divided them into 2 groups by the development of decompression retinopathy. Various factors were compared between the 2 groups, including age, sex, glaucoma type, previous intraocular surgery, preoperative and postoperative intraocular pressure (IOP), refractive error, axial length, anesthesia, and systemic risk factors such as history of diabetes, hypertension, medication, and a laboratory examination including hemoglobin, hematocrit, platelet, prothrombin time (PT), and activated partial thromboplastin time (aPTT). RESULTS:: The proportion of eyes with decompression retinopathy was 3.05% (21 of 688 eyes), and 50 control eyes were selected randomly. No significant differences were observed between the 2 groups in age, systemic disease, glaucoma type, anesthesia, or the incidence rate of postoperative hypotony. Preoperative maximum IOP and the change of IOP was higher (P=0.016, 0.019), PT and aPTT was prolonged (P=0.001, 0.006), and hemoglobin and hematocrit level was lower in patients with decompression retinopathy (P=0.005, 0.002). Preoperative maximum IOP and hemoglobin showed a significant association with the development of decompression retinopathy in a multivariate analysis (P=0.031, 0.040). No significant difference was observed between the 2 groups for IOP and visual acuity 6 months postoperatively. CONCLUSIONS:: Patients with high preoperative IOP and hematologic abnormalities have a higher risk for developing decompression retinopathy. Surgeons should carefully assess the potential risks and pay close attention to sufficiently decrease IOP preoperatively.

Ammonia Volatilization from Rice Paddy Soils Fertilized with 15N-Urea Under Elevated CO2 and Temperature

( Sang Sun Lim ),( Jin Hyeob Kwak ),( Dong Suk Lee ),( Sun Il Lee ),( Hyun Jung Park ),( Han Yong Kim ),( Hong Shik Nam ),( Kyeong Min Cho ),( Woo Jung Choi ) 한국환경농학회 2009 한국환경농학회지 Vol.28 No.3

It has widely been observed that the effect of elevating atmospheric CO2 concentrations on rice productivity depends largely on soil N availabilities. However, the responses of ammonia volatilization from flooded paddy soil that is an important pathway of N loss and thus affecting fertilizer N availability to concomitant increases in atmospheric CO2 and temperature has rarely been studied. In this paper, we first report the interactive effect of elevated CO2 and temperature on ammonia volatilization from rice paddy soils applied with urea. Urea labeled with 15N was used to quantitatively estimate the contribution of applied urea-N to total ammonia volatilization. This study was conducted using Temperature Gradient Chambers (TGCs) with two CO2 levels [ambient CO2 (AC), 383 ppmv and elevated CO2 (EC), 645 ppmv] as whole-plot treatment (main treatment) and two temperature levels [ambient temperature (AT), 25.7℃ and elevated temperature (ET), 27.8℃] as split-plot treatments (sub-treatment) with triplicates. Elevated temperature increased ammonia volatilization probably due to a shift of chemical equilibrium toward NH3 production via enhanced hydrolysis of urea to NH3 of which rate is dependent on temperature. Meanwhile, elevated CO2 decreased ammonia volatilization and that could be attributed to increased rhizosphere biomass that assimilates NH4+ otherwise being lost via volatilization. Such opposite effects of elevated temperature and CO2 resulted in the accumulated amount of ammonia volatilization in the order of ACET>ACAT>ECET>ECAT. The pattern of ammonia volatilization from applied urea-15N as affected by treatments was very similar to that of total ammonia volatilization. Our results suggest that elevated CO2 has the potential to decrease ammonia volatilization from paddy soils applied with urea, but the effect could partially be offset when air temperature rises concomitantly.

A new catalyst-embedded hierarchical air electrode for high-performance Li–O₂ batteries

Lim, Hee-Dae,Song, Hyelynn,Gwon, Hyeokjo,Park, Kyu-Young,Kim, Jinsoo,Bae, Youngjoon,Kim, Hyungsub,Jung, Sung-Kyun,Kim, Taewoo,Kim, Yong Hyup,Lepró,, Xavier,Ovalle-Robles, Raquel,Baughman, Ray H. The Royal Society of Chemistry 2013 ENERGY AND ENVIRONMENTAL SCIENCE Vol.6 No.12

<P>The Li–O<SUB>2</SUB> battery holds great promise as an ultra-high-energy-density device. However, its limited rechargeability and low energy efficiency remain key barriers to its practical application. Herein, we demonstrate that the ideal electrode morphology design combined with effective catalyst decoration can enhance the rechargeability of the Li–O<SUB>2</SUB> battery over 100 cycles with full discharge and charge. An aligned carbon structure with a hierarchical micro-nano-mesh ensures facile accessibility of reaction products and provides the optimal catalytic conditions for the Pt catalyst. The new electrode is highly reversible even at the extremely high current rate of 2 A g<SUP>−1</SUP>. Moreover, we observed clearly distinct morphologies of discharge products when the catalyst is used. The effect of catalysts on the cycle stability is discussed.</P> <P>Graphic Abstract</P><P>The Li–O<SUB>2</SUB> battery holds great promise as an ultra-high-energy-density device. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c3ee41910j'> </P>

Investigation of Magnetic Properties of Zn Doped Y-Type Barium Ferrite

Jung Tae Lim,Chul Sung Kim IEEE 2013 IEEE transactions on magnetics Vol.49 No.7

<P>The Ba<SUB>2</SUB>Co<SUB>2-x</SUB>Zn<SUB>x</SUB>Fe<SUB>12</SUB>O<SUB>22</SUB> (x = 0.0$, 0.5, 1.0, 1.5, and 2.0) polycrystalline samples were synthesized by the solid-state reaction method. Based on the Rietveld refinement, the crystal structures of samples were found to be single-phased and determined to be rhombohedral with space group of R - 3m. The unit cell volume (V<SUB>u</SUB>) of the samples increased with increasing Zn ion concentration. In the magnetic phase diagram of Ba<SUB>2</SUB>Co<SUB>2-x</SUB>Zn<SUB>x</SUB>Fe<SUB>12</SUB>O<SUB>22</SUB> system, samples with x ≤ 1.5 showed the spin transitions both at T<SUB>s</SUB> and T<SUB>C</SUB>. For x = 2.0 sample, we observed the disappearance T<SUB>s</SUB>. The non-magnetic Zn ions preferentially occupy the tetrahedral sublattices of 6c<SUB>IV</SUB>*, and 6c<SUB>IV</SUB>* with down-spin site. At low temperature, this leads to the increase in M<SUB>s</SUB>. However, the experimentally measured M<SUB>s</SUB> at 295 K shows a slight decrease around x = 1.5. In - ddition, we have measured Mössbauer spectra of samples at various temperatures ranging from 4.2 to 750 K, which can analyzed with six-sextets for Fe sites corresponding to the 3b<SUB>VI</SUB>, 6c<SUB>IV</SUB>*, 6c<SUB>VI</SUB>, 18h<SUB>VI</SUB>, 6c<SUB>IV</SUB>, and 3α<SUB>VI</SUB> of the Y-type hexagonal crystallographic sites. From the Mössbauer spectra of 295 K, we observed the line-width broadening with increasing Zn concentration.</P>

Poster Session : Effect of Cudrania tricuspidata extract to enhance immune responses

( Sung Sook Kim ),( Kyung Bok Lee ),( Jung Lim Lee ),( Yung Choon Yoo ) 생화학분자생물학회 2006 생화학분자생물학회 춘계학술발표논문집 Vol.2006 No.-

Wedelolactone inhibits adipogenesis through the ERK pathway in human adipose tissue‐derived mesenchymal stem cells

Lim, Seyoung,Jang, Hyun‐,Jun,Park, Eun Hee,Kim, Jung Kuk,Kim, Jung‐,Min,Kim, Eung‐,Kyun,Yea, Kyungmoo,Kim, Yun‐,Hee,Lee‐,Kwon, Whaseon,Ryu, Sung Ho,Suh, Pann‐,Ghill Wiley Subscription Services, Inc., A Wiley Company 2012 Journal of cellular biochemistry Vol.113 No.11

<P><B>Abstract</B></P><P>Wedelolactone is an herbal medicine that is used to treat septic shock, hepatitis and venom poisoning. Although in differentiated and cancer cells, wedelolactone has been identified as anti‐inflammatory, growth inhibitory, and pro‐apoptotic, the effects of wedelolactone on stem cell differentiation remain largely unknown. Here, we report that wedelolactone inhibits the adipogenic differentiation of human adipose tissue‐derived mesenchymal stem cells (hAMSCs). Wedelolactone reduced the formation of lipid droplets and the expression of adipogenesis‐related proteins, such as CCAAT enhancer‐binding protein‐α (C/EBP‐α), peroxisome proliferator‐activated receptor‐γ (PPAR‐γ), lipoprotein lipase (LPL), and adipocyte fatty acid‐binding protein aP2 (aP2). Wedelolactone mediated this process by sustaining ERK activity. In addition, inhibition of ERK activity with PD98059 resulted in reversion of the wedelolactone‐mediated inhibition of adipogenic differentiation. Taken together, these results indicate that wedelolactone inhibits adipogenic differentiation through ERK pathway and suggest a novel inhibitory effect of wedelolactone on adipogenic differentiation in hAMSCs. J. Cell. Biochem. 113: 3436–3445, 2012. © 2012 Wiley Periodicals, Inc.</P>