H9c2 심근 세포주에서 외인성 nitric oxide가 허혈에 의한 세포 독성에 미치는 영향

정성구,장현용,김명천,고영관,정주호,배영미,박원서,김대중,유영민,김성수,임성빈 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Background: Nitric oxide(NO) is known to have protective effects on an ischemic heart and to exert triggering effects on ischemic preconditioning. However, the effects of NO during the ischemic period have not been investigated. To investigate the role of exogenous nitric oxide in a model of ischemic heart cell death, we studied the effects of ischemic preconditioning and ischemia in a normal and an ischemic buffer. Methods: Rat cardiac myoblast cells(H9c2) were cultured in a normal and an ischemic buffered medium. For the ischemic culture of heart cells, the cells were cultured in a dessicator with GasPak for 5 hrs. In ischemic preconditioning, the cells were pretreated with ischemic buffer for 5 min and then perfused with normal medium for 30 min. For the measurement of the cytotoxicity, a MTT(3-4-Sdimethylthiazol-2-yl-2,5-diphenyl tetrazolium bromide) assay was performed. A DAPI(4',6-diamidino-2-phenylindole dihydrochloride) staining procedure and a flow cytometry analysis were performed to confirm apoptotic cell death by ischemia. Results: Cell viability, as determined by using a MTT assay, showed that the preconditioned group treated with NO showed more cell death than with the not-preconditioned groups in both normal and ischemic buffers. But, In normal medium and not-preconditioned groups, NO showed protective effect according to the concentrations(100,1000μM) . No treatment with NO produced the different results. In normal medium, the protective effect of ischemic preconditioning was demonstrated, but no protective effect of ischemic preconditioning could be seen in the case of the ischemic buffer. The DAPI staining and flow cytometry analysis of heart cells showed characteristic apoptotic features. Conclusion: NO added in the ischemic phase had deterious effects on heart cells. Ischemic preconditioning was more harmful than ischemia alone. The toxicity of the cells was characteristic apoptosis.

급성 허혈성 뇌졸중에 대한 동맥내 혈전 용해술 후 발생한 혈복증 1예

정성구,김명천,고영관 대한응급의학회 2001 대한응급의학회지 Vol.12 No.2

Although thrombolytic therapy is one of the most effective therapeutic option for acute ischemic stroke, hemorrhagic complication still remains major concern about its application. A patient with hemoperitoneum, who previously underwent thrombolytic therapy(intraarterial urokinase infusion, IAUK) for acute ischemic stroke was treated successfully with emergency transarterial embolization for rupture of hepatocellular carcinoma. We present a rare case of rupture of hepatocellular carcinoma after intraarterial urokinase infusion for acute ischemic stroke with brief review of the literature.

패혈증과 피부 감염증 의증으로 오진된 스티븐스 존슨 증후군 1예

정성구,김명천,고영관 대한응급의학회 2001 대한응급의학회지 Vol.12 No.4

Stevens-Johnson syndrome(SJS) is a rare, life-threatening, drug-induced cutaneous reaction which presents dramatic, unforgettable manifestations. While the characteristic features of the disease are well known universally, many physicians have not seen a case of SJS which may result in delay in recognition and treatment. We report a rare case of SJS, which was initially misiagnosed as sepsis with skin infection and atypical pneumonia on emergency department presentation, and we give a brief review of the literature.

중소규모 제조업체 근로자의 인구·사회학적 특성이 건강행동변화단계에 미치는 영향

구명회,임현우,박정일,이강숙,구정완 大韓産業醫學會 2002 대한직업환경의학회지 Vol.14 No.1

목 적 : 근로자 건강증진을 위한 사업장 건강증진 사업 프로그램 기획실에 건강 위험요인을 평가하고 근로자 특성에 4따른 행동변화단계를 조사하여 행동 변화단계에 따른 성공적인 근로자 건강증진전력을 위한 기초자료를 제공하고자 본 연구를 진행하였다. 방 법 : 경인지역이 중소 기업 제조업체 근로자 282명을 대상으로 설문조사를 수행하였으며, 일반적 특성에 대한 조사, 건강위험평가, 위험요인에 대한 행동변화단계를 조사하였다. 결 과 : 건강위험평가에서 흡연자는 남자 근로자에 서 65.7 %, 규칙적인 운동을 하지 않는 남자 근로자는 70.5 %, 여자 근로자는 86.1 %이었고 음주는 남자 근로자의 78.6 %, 여자 근로자인 50 %이었고 과음은 남자 근로자의 29.5 %, 여자 근로자의 11.1 %이었다. 건강한 생활습관으로의 행동변화단계로 계획전단계, 계획 단계, 실천단계, 유지단계 등 4개의 단계로 보면 근로자에서의 금연에 대하여 각각 40.5 %, 23.7 %, 12.1 % 및 23.7 %, 운동에 대하여는 18.4 %, 37.6 %, 17.7 % 및 26.3 %, 음주하였던 사람중 절주에 대하여는 20.9 %, 15.4 %, 10.0 % 및 53.7 %이었다. 흡연을 하는 보건관리자의 행동단계가 일반근로자 보다 유의하게 많았다. 운동에 대해서는 근로자중 50세 이상과 생산직 근로자에서 계획전단계가 다른 근로자보다 유의하게 많았다. 결 론 : 이상의 결과로 대부분의 근로자가 금연을 제외하고는 건강한 생활습관으로의 행동변화를 원하는 것을 알 수 있었으며 근로자의 특성에 따라 행동 변화단계가 다르므로 이를 고려한 다양한 건강증진 프로그램을 개발함으로써 참여율 및 성공율을 높일 수 있으리라 사료된다. Objectives : This research attempts to investigate the relationship between health risk factors and workers' behavioral changes based on individual worker's characteristics to allow the development of more effective health promotion programs in businesses places. Methods : A questionnaire survey was conducted with 282 workers employed in small to medium sized enterprises in the Kyong-in area of Korea. The questionnaire was composed of general characteristics, a health risk assessment and an evaluation of the stage of behavior change for health promotion strategy in the workplace. Results : The health risk assessment revealed that 65.7 % of male workers smoke, 70.5% of male workers do not exercise on a regular basis, 86.1 % of female workers do not exercise at all, and 78.6 % of male workers and 50 % of female workers drink alcohol. In addition, the survey identified that 29.5% of male workers are addicted to excessive drinking, as are 11.1% of female workers. Based on the four stages of behavioral changes that lead to health, the pre-contemplation stage, contemplation stage, action stage, and maintenance stage, 40.5 %, 23.7 %, 12.1 % and 23.7 % of workers are in these stages, respectively, with regards to quitting smoking. As for regular exercises, 18.4 %, 37.6 %, 17.7 %, and 26.3 % of workers belong to each stage, respectively. As for moderation in drinking, 20.9 %, 15.4 %, 10.0 %, and 53.7 % are in each stage, respectively. The rate of health managers in the active stage of quitting smoking is significantly higher than that of general workers. Among the workers who do not exercise on a regular basis, workers over 50 years-old and blue color workers are more common in the precontemplation stage than any other worker groups. Conclusions : The results of this study suggest that the stages of behavioral change vary with worker characteristics. An awareness of the effects of the high risk factors is essectial for promoting workers' attendance in health promotion programs.

근골격계 악성 종양치료에서 동소 자가골 이식술을 이용한 사지 구제술

강용구,우영균,김정만,문영상,송영오 가톨릭 중앙의료원 가톨릭 암센터 1993 가톨릭 암센터 論集 Vol.7 No.1

청각 자극용 전기자극기 개발

허승덕,정동근,김리석,김광년,강명구,김재룡,김기련 한국음성과학회 2004 음성과학 Vol.11 No.3

This paper introduces a development of an electrical stimulator for auditory stimulation. The electrical stimulator is useful in neurotological diagnosis, audiological evaluation, candidate selection for cochlear implantation, optimal device selection and decision making of MAP strategy for severe-to-profound hearing impaired persons. The development was based on sound parameters of auditory brainstem responses and auditory electrophysiological characteristic such as effective firing of auditory nerve and recording evoked potentials during refractory period of neuron. Besides pulse parameter could adjustable by programming for more varied electrical stimulation evoked response audiometry. Using the electrical stimulator, electrical square pulse was applied to promontory, and electrically evoked auditory brainstem response and electrically middle latency response were successfully recorded in cats.

조기유자망 어구의 개량 및 생력화 조업시스템 개발 : 3. 생력형 5단 양망기의 해상 성능 시험

김석종,구명성,강경범,서대범,정용진 제주대학교 해양과환경연구소 2005 해양과환경연구소 연구논문집 Vol.29 No.2

와우 갑각 전기자극 뇌간유발반응용 원추형 전극의 개발

허승덕,정동근,강명구,김리석,고도흥 한국음성과학회 2003 음성과학 Vol.10 No.4

This paper introduces a new zinc coated copper wire electrode with coiled cone shape which has low surface resistance and tolerance to the motion artifact for promontory stimulation electrically auditory brainstem responses (PSEABR). Auditory brainstem responses (ABR) can be used to predict hearing threshold level with a great deal of accuracy particularly for a young child who cannot cooperate mechanically and some hearing impaired who are exaggerating a hearing loss for economic compensation. While severe profound sensorineural hearing losses may not be implemented by auditory potentials, PSEABR is proven as a useful tool even for some sensorineural related hearing impaired. It was shown that PSEABR gives the electrical stimuli to promontory of the cochlear instead of giving acoustic stimuli. For this reason, PSEABR can be used as an alternative for cochlear implantation, and can also be used as an optimal device selection and neural information for MAP. It was found that the role of electrode is very important in PSEABR. Even though this cone-shaped electrode was applied in animal experiments, waveforms are well produced by PSEABR. Thus, it was concluded that cone-shaped electrode turned out to be a useful preoperative audiological evaluation tool in deciding time for cochlear implantation surgery.

MOCVD법에 의한 광 통신용 1.55㎛ PBH-DFB 레이저 다이오드 제작

오환술,김중연,전현성,강명구 건국대학교 산업기술연구원 1997 건국기술연구논문지 Vol.22 No.-

DC-PBHDFB LD operating at 1.55㎛ were fabricated by 3-step MOCVD process. The first-order grating with 2370Å period and 700Å etching depth was formed by holographic lithography and wet etching. Fabricated DCPBH-DFB LD operates in single longitudinal mode with side-mode suppression of more than 35dB and the lasing wavelength of 1.547㎛ at room temperature and 5mW, and the lasing threshold current and the slope efficiency were 15.6mA and 0.15mW/mA, respectively. The characteristic temperature of threshold current(To) and the temperature dependence of the lasing wavelength were obtained 57.3K and 1.0Å/℃ from the DFB laser between 20℃ and 70℃, respectively.

보건관리 대행사업 평가도구의 타당성, 신뢰도 및 인과관계 검증에 관한 연구

박정일,조병희,구정완,김광종,김현욱,원종욱,이명숙,정문희 가톨릭대학교 산업의학센터 2001 韓國의 産業醫學 Vol.40 No.1

In order to verify the validity, reliability and relativeness of preliminary evaluation tool for the group occupational health services(GOHS), a questionnaire survey for doctors, nurses and hygienists of 18 GOSH institutions, and employers, health managers and employees of 340 enterprises was conducted. A factor analysis and correlation test for the statistical validity of the variables in the evaluation tool, a Chronbach's Alpha value for the reliability in the evaluation indices and a regression analysis for the relations of the evaluation model were performed. The results were as follows: 1.In factor analysis, thirty-three variables among 43 variables were selected as significant variables, ie 9 out of 11 for input index, 11 out of 14 for throughout index and 13 out of 18 for out put index. 2.Three evaluation(outcome) scales(evaluation by employer, health manager and employee) which composed of 16 identical items were used. 3.The Chronbach's Alpha values of input, throughout, out put and outcome indices were 0.5221, 0.5473, 0.7122 and 0.8546 respectively. 4.Regression equations of independent variables on the evaluation scales of employer, health manager and employee were all statistically significant.