Numerical Investigation and Design of Cold-formed Steel Angle Columns with Complex Edges under Axial Compression

Jun-Feng Zhang,Mengmeng Feng,En-Feng Deng,Shiyun Pang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.2

Currently, cold-formed steel (CFS) is widely used in fabricated steel structures for high strength-to-weight ratio and ease for installation. However, limited research is available in the literature on the CFS angle columns with complex edges under axial compression. In this paper, the axial compressive capacity of the CFS angle columns with complex edges was numerically studied. The distortional buckling characteristics of 1008 CFS angle sections with complex edges were analyzed by finite strip software CUFSM. Detailed non-linear finite element model (FEM) was developed based on finite element program ABAQUS and verified by comparing with test results. The verification indicated that the developed FEM could well predict the ultimate strength and failure model of the specimens. Using the validated FEM, an extensive parametric study comprised of 330 models was conducted. The influences of the specimen length, the edge ratio and the limb-width ratio of the angle section on the axial compressive capacity of CFS angle columns with complex edges were evaluated. Then an improved direct strength method (DSM) was proposed based on the existing DSM. The evaluations based on tests and FEA results showed that using the modified DSM to predict the load-bearing capacity of the CFS angle columns with complex edges was feasible.

Macrophages Promote Coal Tar Pitch Extract-induced Tumorigenesis of BEAS-2B Cells and Tumor Metastasis in Nude Mice Mediated by AP-1

Zhang, Peng,Jin, Yue-Fei,Zhang, Qiao,Wu, Yi-Ming,Wu, Wei-Dong,Yao, Wu,Wu, Yong-Jun,Li, Zhi-Tao,Zhao, Yong,Liu, Yu,Feng, Fei-Fei Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: We sought to evaluate the role of tumor associated macrophages (TAMs) on the promotion of coal tar pitch extract (CTPE)-induced tumorigenesis of human bronchial epithelial cells (BEAS-2B) and tumor metastasis in nude mice, and related mechanisms. Materials and Methods: BEAS-2B cells were first treated with 2.4 mg/mL CTPE for 72 hours. After removal of CTPE, the cells were continuously cultured and passaged using trypsin-EDTA. THP-1 cells were used as macrophage-like cells. BEAS-2B cells under different conditions (n=6/group) were injected into the back necks of nude mice, and alterations of tumor xenograft growth, indicative of tumorigenicity, and tumor metastasis were determined. Pathological changes (tumor nests and microvascular lesions) of HE-stained tumor tissues were also evaluated. The expression of AP-1(c-Jun) in xenografts and metastatic tumors was determined using immunohistochemistry. Results: Tumor size and weight in nude mice transplanted with the mixture of CTPE-induced passage 30 BEAS-2B and THP-1 cells (2:1) were increased compared to those from the CTPE-treated BEAS-2B cells at passage 30 alone at different observation time points. Tumor metastasis to lymph nodes and liver was only detected after transplantation of a mixture the two kinds of cells. The numbers of tumor nests and microvascular lesions, and the expression levels of AP-1 (c-Jun) in tumors from the mixture of two kinds of cells were increased apparently in contrast to those in tumor from the CTPE-treated BEAS-2B cells of passage 30 alone. In addition, there was positive correlation between AP-1 (c-Jun) expression level and the number of microvascular lesions, or between AP-1 (c-Jun) expression level and tumor metastasis in these two groups. Conclusions: TAMs not only facilitate tumorigenesis transformation of CTPE-induced BEAS-2B cells, but also promote tumor growth, angiogenesis and metastasis in nude mice in vivo, which may be mediated by AP-1.

중국 형법의 일반상황

Feng Jun 한국형사법학회 2010 한국형사법학회 학술대회 논문집 Vol.2010 No.3

Mechanism of the simultaneous removal of nitrate and Ni(II) by Enterobacter sp. CC76 through mixotrophic denitrification processes

Jun Feng Su,Shu Yang,Ting Lin Huang,Xue Chen Bai,Jin Suo Lu,Lei He,Min Li 한국화학공학회 2019 Korean Journal of Chemical Engineering Vol.36 No.7

We studied the mechanism for the simultaneous removal of nitrate and Ni(II) by Enterobacter sp. CC76. Response surface methodology results showed that the maximum removal ratios of nitrate and Ni(II) were 95.02% and 75.99% under the following conditions: pH 7.37, 54.31mg·L1 Fe(II), and 10.00mg·L1 Ni(II). The mechanism of Ni(II) removal involved Fe-oxide adsorption and the increase of pH. In addition, meteorological chromatography analysis indicated that Ni(II) affected gas composition during denitrification. Scanning electron microscopy and X-ray photoelectron spectroscopy confirmed that Fe-oxide adsorption was the main contributor to Ni(II) removal. This study shows that Enterobacter sp. CC76 can enhance the adsorption of Ni(II) onto Fe-oxides while removing nitrate.

A novel M2e-multiple antigenic peptide providing heterologous protection in mice

Feng Wen,Ji-Hong Ma,Hai Yu,Fu-Ru Yang,Meng Huang,Yan-Jun Zhou,Ze-Jun Li,Xiu-Hui Wang,Guo-Xin Li,Yi-Feng Jiang,Wu Tong,Guangzhi Tong 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1

Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.

Calcium precipitation to remove fluorine in groundwater: Induced by Acinetobacter sp. H12 as a template

Jun Feng Su,Ruijie Zhang,Xiaofen Hu,Amjad Ali,Zhao Wang 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.3

Calcium precipitation induced by Acinetobacter sp. H12 can simultaneously remove F− and Ca2+. Adsorption kinetics and isotherm studies showed that the defluoridation effect improved with the increase of temperature and time. The equilibrium adsorption capacity of calcium precipitation was 7.43 mg g−1 at the initial F− concentration of 10 mg L−1. Moreover, as the pH increased, the F− adsorption capacity decreased and the Ca2+ removal rate gradually increased. The adsorption process was highly fitted to the pseudo-second-order and the Freundlich isotherm. Adsorption thermodynamics analysis demonstrated that the adsorption process was a spontaneous endothermic reaction. The activation adsorption energy was 2.81 kJ mol−1 and the frequency coefficient was 10.88 h−1. EEM, SEM, XPS. XRD results indicated that the defluoridation mechanism may be due to adsorption and co-precipitation. The mechanism of Ca2+ removal may be co-precipitation with F−. It is worth-mentioning that calcium precipitation overcame the problem of adding carbon sources and avoided the microbial safety risk in the effluent. Therefore, a new insight was proposed for simultaneous remediation of Ca2+ and F− from groundwater combining the biological and chemical method.

Neuron Discharge and c-Fos Expression in the Nucleus of the Solitary Tract Following Electroacupuncture at Acupoints of the Yangming Stomach Meridian of Foot

Jun-Feng He,Jie Yan,Jiang-Shan Li,Jian-Hua Liu,Chao Wang,Xiao-Rong Chang,Ya-Ting Qu 사단법인약침학회 2013 Journal of Acupuncture & Meridian Studies Vol.6 No.2

The nucleus of the solitary tract (nucleus tractus solitarii; NTS) is a primary center for both visceral afferents and somatic afferents. Previous experiments have demonstrated that the NTS is closely connected to the stomach and acupoints in the Yangming Stomach Meridian of Foot (ST Meridian). In this study, extracellular recording and immunochemistry methods were used to analyze the discharge of neurons and c-Fos protein expression in the NTS following acupuncture at different acupoints and a nonacupoint. A total of 104 discharging neurons were detected in the NTS of 52 rats, of which 86 provided complete data. After acupuncture at Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint, the neuron response rate in the NTS was 65.12%, 51.16%, 46.51%, 34.88% and 31.40% respectively. For neuron response rate, there was a significant difference among Sibai (ST 2), Zusanli (ST 36), Neiting (ST 44), Quanliao (SI 18), and the nonacupoint (p < 0.01 or p < 0.05). In the other 48 rats, the number of c-Fos immunoreactive neurons in the NTS by electroacupuncture (EA) at Sibai (ST 2) group was significantly higher than that EA at other acupoints and the nonacupoint (p < 0.05 or p < 0.01). EA at both Zusanli (ST 36) and Neiting (ST 44) increased c-Fos immunoreactive neurons significantly over EA at Quanliao (SI 18) and the nonacupoint (p < 0.05 or p < 0.01), while there was no difference between EA at Quanliao (SI 18) and the nonacupoint group (p > 0.05). The experiments demonstrated that the afferent convergence in NTS are different by body surface points stimulus, which suggests that the NTS might be a primary center in the central nervous system receiving acupoints stimulus from the ST Meridian.

Expression patterns of octoploid strawberry TGA genes reveal a potential role in response to Podosphaera aphanis infection

Jun Feng,Yang Cheng,Caixia Zheng 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

TGA family transcription factors are downstream components of the salicylic acid (SA) pathway and play a crucial role in the penetration defense against powdery mildew, a disease caused by the biotrophic pathogen Podosphaera aphanis that can cause enormous financial losses. However, the TGA genes in the octoploid strawberry (Benihoppe) remain largely unexplored. In this study, 11 FaTGA genes were identified and grouped into five subgroups based on the Strawberry GARDEN database. The evolution of TGAs has been relatively conservative, maintaining nearly the same protein domain and motif architectures. RT-qPCR showed that FaTGA s have different expression patterns in roots, stems, and leaves. The characteristics of their gene expression patterns indicate that FaTGA1, FaTGA2, FaTGA5, FaTGA7, FaTGA8, FaTGA9, and FaTGA10, as well as SA-responsive genes, were responsive to P. aphanis infection. In particular, exogenous SA induced significant upregulation of FaTGA and SA-responsive genes. Further correlation analysis suggested that FaTGA1, FaTGA2, FaTGA5, FaTGA7, FaTGA8, and FaTGA10 may play a crucial role in the response to powdery mildew disease. Taken together, our results reveal the responses of TGA genes in the strawberry P. aphanis interaction, which will provide further insight into the strawberry defense against powdery mildew.

The Action Research of Game Sense on Elementary School Soccer Team Training

Jun-Feng Zhong,Yuh-Chih Chen 한국스포츠교육학회 2011 한국스포츠교육학회 국제학술대회 자료집 Vol.2011 No.12

Prevalence of Spina Bifida Occulta and Its Relationship With Overactive Bladder in Middle-Aged and Elderly Chinese People

Jun Wei Wu,Yu Rong Xing,Yi Bo Wen,Tian Fang Li,Quan De Feng,Xiao Ping Shang,Yun Long Li,Jin Jin Feng,Xin Xin Wang,Rong Qun Zhai,Xiang Fei He,Tao Chen,Xin Jian Liu,Jian Guo Wen 대한배뇨장애요실금학회 2016 International Neurourology Journal Vol.20 No.2

Purpose: To investigate the prevalence of spina bifida occulta (SBO) and its relationship with the presence of overactive bladder (OAB) in middle-aged and elderly people in China. Methods: A cross-sectional community-based survey was carried out at 7 communities in Zhengzhou City, China from December 15, 2013 to June 10, 2014, where residents aged over 40 years were randomly selected to participate. All of the participants underwent lumbosacral radiographic analysis and relevant laboratory tests. A questionnaire including basic information, past medical history and present illness, and the OAB symptom score was filled out by all participants. Chi-square tests and logistic regression were used for data analysis with a P-value of <0.05 denoting statistical significance. Results: A total of 1,061 subjects were qualified for the final statistical analysis (58.8±11.7 years; male, 471 [44.4%]; female, 590 [55.6%]). The overall prevalence of SBO was 15.1% (160 of 1,061): 18.3% (86 of 471) in men and 12.5% (74 of 590) in women. Among these subjects, 13.7% (145 of 1,061) had OAB: 13.2% (62 of 471) in men and 14.1% (83 of 590) in women. The results of logistic regression showed that age, SBO, history of cerebral infarction (HCI), and constipation were risk factors for OAB (P<0.05), while sex, history of childhood enuresis (HCE), body mass index (BMI), and diabetes mellitus (DM) were not (P>0.05). In men, age, SBO, and constipation were risk factors for OAB (P<0.05), while HCE, BMI, DM, HCI, and benign prostate hyperplasia were not (P>0.05). In women, age, SBO, and HCI were risk factors for OAB (P<0.05), while HCE, BMI, DM, vaginal delivery, and constipation were not (P>0.05). Conclusions: The prevalence of SBO is high and it is related to OAB in middle-aged and elderly people in China.