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BAK, HANA,LEE, HYUN JU,CHANG, SUNG-EUN,CHOI, JEE-HO,KIM, MYEUNG NAM,KIM, BEOM JOON Blackwell Publishing Inc 2009 Dermatologic surgery Vol.35 No.8
<P>BACKGROUND</P><P>Characteristic clinical features of melasma are symmetry of hyperpigmentation and distribution related to trigeminal nerves, which suggest that the neural involvement could play a part in the pathogenic mechanisms of pigmentation.</P><P>OBJECTIVE</P><P>To evaluate whether some neuropeptides and neurotrophins and their receptors were associated with the pathogenesis of melasma.</P><P>METHODS</P><P>To investigate the involvement of neuronal system and neuropeptides in melasma, we examined the expression of nerve growth factor receptor (NGFR) and neural endopeptidase (NEP) in melasma lesional and nonlesional skin. Skin biopsies were obtained from lesional and nonlesional facial skin of six Korean women with melasma. Confocal laser scanning microscopic examination and western blot were performed.</P><P>RESULTS</P><P>Melasma lesions showed markedly greater expression of NGFR and NEP than nonlesional skin.</P><P>CONCLUSION</P><P>We suggest that neuroactive molecules, including NGF, is one of the critical factors for the pathogenesis of melasma, which may directly affect the microenvironment around melanocytes through a NGFR immunoreactivity (NGFR-IR) nerve fiber pathway, and higher levels of NEP in melasma has an important role in regulation of melanogenesis.</P>
Bak Su-Bin,Choi Hansol,Kim Gyoung-Deuck,Kim Ju Gyeong,Kwon Da-Ae,Kim Ha-Young,Son Dong-Won,Jeong Jang-Hun,Lee Byung-Woo,An Hyo-Jin,Lee Hak Sung 한국독성학회 2024 Toxicological Research Vol.40 No.2
HemoHIM G is a functional food ingredient composed of a triple herbal combination of Angelica sinensis, Ligusticum chuanxiong, and Paeonia lactiflora, to improve impaired immune function. Considering the pharmacological benefits of its constituent herbal components, HemoHIM G is anticipated to have various health benefits; however, its toxicity has not been thoroughly evaluated. Here, we conducted a comprehensive study to assess the safety of HemoHIM G in terms of acute oral toxicity, 13-week repeat-dose toxicity, and genotoxicity. In the oral acute toxicity study, Sprague–Dawley rats were orally administered a single dose of HemoHIM G at 5000 mg/kg/day, the limit dose for the acute study. No abnormal findings or adverse effects were observed in this study, as confirmed by gross pathology. A 13-week repeated-dose toxicity study was conducted with HemoHIM G at doses of 1250, 2500, and 5000 mg/kg/day to examine the subchronic toxicity in both male and female rats after 28 days of dose-range finding study. No test substance-related clinical signs or mortality was observed at any of the tested doses. Gross pathology, hematology, blood chemistry, and histopathology were within normal ranges, further supporting the safety of HemoHIM G. Therefore, the NOAEL of HemoHIM G was considered to be at 5000 mg/kg/ day for both sexes of rats. Bacterial reverse mutation tests, a chromosome aberration test in human peripheral blood lymphocytes, and a mouse micronuclei test were conducted to identify the potential genotoxicity of HemoHIM G. HemoHIM G is non-mutagenic and non-clastogenic. Collectively, these findings provide valuable evidence for the safe use of HemoHIM G as a functional food ingredient.
Ahn, Sang Jung,Bak, Hye Jin,Park, Ju Hyeon,Lee, Jin Young,Kim, Na Young,Han, Jin Woo,Jo, Hyae In,Chung, Joon Ki,Lee, Hyung Ho Humana Press 2013 Applied biochemistry and biotechnology Vol.170 No.5
<P>Cystatins are endogenous inhibitors of mammalian lysosomal cysteine proteinases, such as cathepsins B, L, H, and S. Cystatin C belongs to the type 2 cystatin family. In this study, the 751-bp cystatin C cDNA (PoCystatin C) of olive flounder (Paralichthys olivaceus) was cloned by screening from the olive flounder cDNA library. The mRNA expression of the PoCystatin C gene was examined in various tissues from normal and lipopolysaccharide (LPS)-stimulated olive flounder by RT-PCR and was compared with inflammatory cytokines IL-1β, IL-6, and IL-8. PoCystatin C transcripts ubiquitously existed in all normal and LPS-stimulated tissues that were tested. The recombinant PoCystatin C protein was expressed in Escherichia coli BL21(DE3) in pCold TF DNA expression vector as a 70-kDa fusion protein. The protease inhibitory activities of recombinant PoCystatin C toward papain cysteine protease, piscine cathepsins (L, S, K, F, and X), and bovine cathepsin B were measured with the synthetic fluorogenic peptide substrates. PoCystatin C tightly inhibited papain cysteine protease, whereas cathepsins L, S, K, F, X, and B were inhibited with lower affinities. Our results indicate that the P. olivaceus cystatin C is a homolog of mammalian cystatin C due to its sequence, structure, tissue expression, and biochemical activity.</P>
이우성 ( Woo Sung Lee ),김건엽 ( Keon Yeop Kim ),이무식 ( Moo Sik Lee ),나백주 ( Bak Ju Na ),조형원 ( Hyong Won Cho ),김현지 ( Hyun Jee Kim ) 경희대학교 경영연구원 2007 의료경영학연구 Vol.1 No.1
Objective: The purpose of this study was to examine the correlations among middle-level manager leadership, leadership satisfaction, and job satisfaction in general hospital employees. Methods: A questionnaire was administered to 578 hospital employees from July to August 2005. The survey pertained to information on the general characteristics of the employees, leadership score, leadership satisfaction, job satisfaction, and a list of requirements for leadership. Results: In terms of the leadership score, females, individuals in their 20s, nursing employees, those who were unmarried, those whose careers were relatively short, and those in private college hospitals rather than in national hospitals or medical corporations, presented higher scores. As for the correlations among leadership, leadership satisfaction, and job satisfaction, all three constructs were related to each other. In multiple regression analysis when the dependent variable was leadership satisfaction, the leadership score, occupation type, and hospital type were significant variables. When job satisfaction was the dependent variable, age, occupation type, and leadership score were significant. The requirements for leadership included: having drive at work, interpersonal skills, a sense of responsibility, decision-making capacity, and tolerance, in the respective order. Conclusion: Hospitals need to analyze the requirements of their staff, in terms of middle-level manager qualities, and then reflect them from within the hospital operation in order to promote the hospital`s development in a reasonable and efficient way. In addition, hospitals must appreciate the importance of middle-level manager leadership, and executives need to assign middle-level managers appropriate positions as well as provide consistent education and training programs so they can contribute to the development of the hospital.
( Yoon Suk Bak ),( Hye Ran Kang ),( Young Kwan Cho ),( Young Sook Park ),( Seong Hwan Kim ),( Yun Ju Jo ),( Byung Kwan Son ),( Sang Bong Ahn ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Backgrounds: Primary intestinal lymphangiectasia (PIL) is a rare disease resulting by lymph leakage into the small bowel lumen. Less than 200 PIL cases have been reported globally after 1961. As lymphatic fi uid contains a lot of protein, fat, and lymphocytes, leakage of lymph will cause hypoproteinemia, hypoalbuminemia, hypogammaglobulinemia, and lymphocytopenia. 2 The most common symptoms of patients with PIL are intermittent diarrhea, nausea, and vomiting. Peripheral edema by protein-losing enteropathy, chylous ascites and chylous pleural effusion are often presented. 3 The diagnosis of PIL should exclude the secondary intestinal lymphangiectasia and be confi rmed by pathlogic fi nding from enteroscopic biopsy or surgical resection. Aims: Our purpose is to investigate the clinical characteristics of PIL, the process of distinguish the PIL from secondary intestinal lymphangiectasia and pathologic confi rmation from enteroscopic biopsy. Case report : A 73-year-old male was admitted for endoscopic submucosal dissection of gastric adenoma without any complaining symptom. In the contrast-enhanced abdominal CT, there are nodularity of inner layer with mucosal enhancement of gastric antrum, and large amount ascites without other clue of malignancy, pancreatitis or cirrhosis. In the peritoneal analysis, the fi uid was chylous ascites and negative result for infi ammation, infection and malignancy using neutrophil count, bacterial culture and cytology. By enteroscopic biopsy, lymphangiectasia was comfirmed. Based on these results, low-fat and medium-chain triglyceride diet started. Discussion : We presented a case of patient with chylous ascites accidentally detected. After exclusion of secondary cause, we confi rmed the PIL by enteroscopic biopsy. We reported a rare intestinal disease in adults.
기미 병변에서 신경 펩티드와 그 수용체의 발현에 대한 연구
이현주 ( Hyun Ju Lee ),박하나 ( Ha Na Bak ),장성은 ( Sung Eun Chang ),최지호 ( Jee Ho Choi ),김명남 ( Myeung Nam Kim ),김범준 ( Beom Joon Kim ) 대한피부과학회 2008 大韓皮膚科學會誌 Vol.46 No.5
Background: The pathogenesis of melasma has not yet been clearly demonstrated. But, clinical manifestations such as remarkable lesional symmetry and the distribution related to trigeminal nerves, suggest that the neural system could play a pathogenic role in melasma. Objective: This study was carried out to examine the expression of some neuropeptides and their receptors, which are well known to be major contributors of neuroinflammation in many dermatoses, in melasma lesions. Methods: Skin biopsies were obtained from the lesional and non-lesional facial skin of 6 Korean women with melasma. Immunofluorecence staining and confocal laser scanning microscopy were performed. Results: In our results, no difference could be detected with regard to the intensity of immunoreactivity for vasoactive intestinal peptide (VIP), calcitonin gene-related peptide (CGRP), calcitonin gene-related peptide receptor (CGRPR), substance P (SP), substance P receptor (SPR), somatostatin (SOM), pituitary adenylate cyclase activating peptide (PACAP) and pituitary adenylate cyclase activating peptide receptor (PACAPR) in the lesional skins compared with the non-lesional skins. Conclusion: These results suggest that neuroinflammation induced by neuropeptides such as substance P, calcitonin gene-related peptide, vasoactive intestinal peptide, and somatostatin and their receptors included in this study, are not directly associated with melasma pathogenesis. (Korean J Dermatol 2008;46(5):627∼632)