Molecular and Cytogenetic Analysis of Transgenic Plants of Rice(Oryza sativa L.) Produced by Agrobacterium-mediated Transformation

Cho,Joon-Hyeong,Kim,Yong-Wook 한국자원식물학회 2004 Plant Resources Vol.7 No.1

To demonstrate the importance of transformation efficiency in independent event, molecular and cytogenetic analysis were conducted with genomic DNA and chromosome of transgenic plants produced by Agrobacterium tumefeciens LBA4404 (pSBM-PPGN: gusA and bar). Selection ratios of putative transgenic calli were similar in independent experiments, however, transformation efficiencies were critically influenced by the type of regeneration media. MSRK5SS-Pr regeneration mediun, which contains 5 mgL$^{-1}$ 수식 이미지 kinetin, 2% (w/v) sucrose in combination with 3% (w/v) sorbitol, and 500 mgL$^{-1}$ 수식 이미지 proline, was efficient to produce transgenic plant of rice from putative transgenic callus in the presence of L-phosphinotricin (PPT). With MSRK5SS-Pr medium, transformation efficincies of Nagdongbyeo were significantly enhanced from 3.7% to 6.3% in independent callus lines arid from 7.3% to 19.7% in plants produced, respectively. Stable integration and expression of bar gene were confirmed by basta herbicide assay, PCR amplification and Southern blotting of bar gene, and fluorescence in situ hybridization (FISH) analysis using pSBM-PPGN as a probe. In Southern blot analysis, diverse band patterns were observed in total 44 transgenic plants regenerated from 20 independent PPT resistant calli showing from one to five copies of T-DNA segments, however, the transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

韓國의 經濟成長과 國民意識의 關聯性에 關한 硏究 : 社會淨化 運動을 中心으로

趙俊衡 단국대학교 경영대학원 1982 經院論叢(壇國大學校) Vol.2 No.-

Southern hybridization에 의한 옥수수α-amylase 遺傳子分析

趙駿衡,金容旭 동국대학교 농림과학연구소 1993 農林科學 論文集 Vol.17 No.-

Total genomic DNA isolation from maize shoot was easier and more efficient than isolation from aleurone layers of seeds. During DNA isolation, it was possible to purify total genomic DNA mays by RNase A and phenol : chloroform extraction without repeated processes. As a result of agarose gel electrophoresis, total genomic DNA of maize was a macromolecule which had more than 23 kilo-base in comparison with the size marker, λ/Hind Ⅲ. DNA fragments digested by BamH Ⅰ, EcoR Ⅰ, and Hind Ⅲ were more than 1 kilo-base and DNA fragments digested with Sau 3A Ⅰ and Taq Ⅰ was less than 1 kilo-base in length. At the same treatment time, the reason of different moving distance of digested DNA fragments, DNA sequance sites recignized and digested with each restriction endonucleases would be specific. DNA fragments by Sau3A Ⅰ and Taq Ⅰ were more migrated than those by BamH Ⅰ, EcoR Ⅰ, and Hind Ⅲ at the same time. Enhanced chemilluminescence (ECL) gene detection system was better than then ^32P― radiolabelling method for α-amylase gene detection. Southern hyridization detection signal was similar to agaross gel electrophoretic band pattern observed. It was observed that binding was specific and α-amylase genes were present in isolate total genomic DNA of maize. Also, maize α-amylase genes might have conserved region which appeared in other cereals, and high level homology would be expected among these genes, maize, and other cereal crops. It seemed that utilization of DNA framents digested by EcoR Ⅰ, Hind Ⅲ, and Sau3A Ⅰ would be mostly efficient in α-amylase genomic DNA cloning.

고지방식을 섭취한 랫드에서 김치유산균의 콜레스테롤 저하효과

조미선, 노정미, 임형준, 한선경, 신명수, 이완규 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.2

본문참고

과민성 대장 증후군 환자에서 Colpermin 투여의 임상 효과

금민수,조창민,김대현,이창형,권영오,김성국,최용환,정준모 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.1

목적 : 과민성 대장 증후군은 장기능 이상의 증상군으로, 인지할 수 있는 기질적 위장관 병태 생리가 없는 경우로 기능적인 장관의 이상 경축과 가장 밀접한 관련이 있을 것으로 생각된다. 그러나 치료면에서는 아직 뚜렷한 방침이 정립되어 있지 않고 다양한 방법들이 제시되고 있는 실정이다. 저자들은 장용성 캅셀로 조제된 박하유 성분인 Colpermin® 의 임상 효과를 평가하고자 본 연구를 시행하였다. 대상 및 방법 : 1996년 2월부터 1996년 11월까지 경북대학교병원 소화기 내과를 방문하여 과민성대장 증후군으로 진단된 34명의 환자를 대상으로 Colpermin® 을 1회 2캅셀씩, 1일 3회, 경구로 2주간 투여하였다. 결과 : 투여전의 증상의 빈도는 복통 88.2%(30/34) 및 설사 73.5%(25/34)를 호소하는 경우가 가장 많았으며, 그 다음으로 변비 50.0%(17/34), 복부 팽만 47.1%(16/34), 고창 38.2%(13/34)순이었다. 약물 투여후 증상별 개선율은 설사를 보였던 환자들에서 호전 11예와 매우 호전 11예로 88.0%(22/25)의 가장 높은 개선율을 보였고, 그 다음으로 복통 73.3%(22/30), 고창 69.2%(9/13), 변비 64.7%(11/17), 복부 팽만 56.3%(9/16)의 순이었다. 종합적인 증상의 개선도는 현저한 개선이 2예(5.9%), 중등도 개선이 9예(26.5%), 약간 개선이 18예(52.9%), 불변이 4예(11.8%), 악화가 1예(2.9%)였으며, 종합적인 유효율은 85.3%(29/34)였다. 약물의 부작용으로 3예의 환자에서 각각 복부 팽만의 발생, 변비의 악화, 복통의 발생이 있었으나, 그 외의 환자들에서는 아무런 부작용도 관찰되지 않았다. 결론 : 과민성 대장 증후군의 약물치료에 있어서 Colpermin®이 유용한 약제가 될 수 있을 것으로 기대된다. Irritable bowel syndrome (IBS) is a disease characterized by a collection of symptoms of intestinal dysfunctions, without any recognized organic gastrointestinal pathology. This study was performed to evaluate clinical effectiveness of peppermint oil (Colpermin®) in the treatment of patients with IBS. 34 patients (18 males and 16 females) with IBS were treated with peppermint oil 0.4ml (as menthol 176㎎) three times daily for 2 weeks and the efficacy of the treatment was evaluated according to the degree of the improvement. The improvement of symptoms was 88.0% (22/25) in diarrhea, 73.3% (22/30) in abdominal pain, 69.2% (9/13) in flatulence, 64.7% (11/17) in constipation, and 56.3% (9/16) in abdominal distension, respectively. Overall effectiveness of subjective symptoms was 85.3% (29/34). Side effects of the drug were noted in only 3 cases, those were aggravation of constipation, development of abdominal distension and abdominal pain. As a result, Colpermin® might be considered as an effective drug in relieving symptoms of IBS.

Long/Short 훈련심볼을 이용하는 개선된 채널추정기법에 의한 IEEE 802.11a 무선 LAN 시스템의 성능 개선

곽재민,정해원,조성준,이형호 한국항행학회 2002 韓國航行學會論文誌 Vol.6 No.3

본 논문에서 IEEE 802.11a 의 규격에 따라 OFDM 무선 LAN 모뎀과 프레임 포맷을 구성하여 시뮬레이션에 의해 BER 성능을 분석하고 수정된 채널 예측 알고리즘을 적용하여 채널 예측 성능이 개선됨을 보인다. 채널 예측에 이용되는 실내 무선채널로서는 AWGN과 TDL모델을 적용한 지연확산채널을 고려한다. 우선 AWGN환경에서 규격에 제시된 전송속도 및 부호율에 따른 OFDM 무선 LAN 시스템의 성능을 분석한다. 다음으로 실내무선 채널 환경에서 채널추정이 불완전한 경우에 대해, 기존의 채널추정방식으로서 프리앰블 내의 2개의 Long 훈련 심볼을 이용하여 채널을 추정하는 경우에 대한 성능을 분석하고, 수수정된 채널 추정기법으로는 IEEE 802.11a의 프리앰블 구조를 변경하지 않으면서 Long 훈련심볼뿐만 아니라 전체 10개의 Shot 훈련심볼 중 8개의 훈련심볼들을 채널추정에 부가적으로 사용하는 방식을 채용한 경우의 성능을 분석하여 기존의 방식과 비교한다. 시뮬레이션 결과의 분석으로부터 수정된 채널 추정방식을 적용하는 경우, 규격에 정의된 프리앰블 구조의 변경없이도 잡음을 억제하는 효과를 향상시킴으로써 채널추정오차가 감소되어 채널추정성능이 개선됨을 확인하였다. In this paper, the BER performance of IEEE 802.11a OFDM WLAN system is obtained by simulation and it is shown that the proposed modified channel estimation algorithm improves the channel estimation performance of the system. The wireless channel used in channel simulation includes AWGN and delay spread channel implemented by TDL model. At first, the performance of OFDM WLAN system according to data rate and coding rate defined in standard is evaluated in AWGN channel. Then, imperfect channel estimation in indoor wireless channel is considered. After the performance of conventional channel estimation scheme using only two long training symbols is evaluated, and that of proposed modified channel estimation scheme using additional 8 short training symbol is compared with it. From the simulation results, it is shown that modified channel estimation scheme provides reduced channel estimation error and improves the channel estimation performance due to noise averaging effect with the same preamble format as defined in specification.

Physiological Responses of Soybean Cultivars to Fusarium soleni f. sp. glycines Causing Sudden Death Syndrome

Joon Hyeong Cho 韓國作物學會 1999 Korean journal of crop science Vol.44 No.4

Six soybean cultivars having different SDS susceptibility were planted with sorghum seedinoculum infested with F. solani isolate 171 in the greenhouse. First leaf symptoms appeared on unifoliar leaves at 9 days after inoculation and all cultivars showed the typical leaf symptoms at 13 days after inoculation, when trifoliar leaves emerged. Leaf symptoms development in susceptible cultivars was faster than in resistant cultivars. Leaf symptom severities during the period of 25 to 29 days after inoculation showed a significant difference between cultivars which had SDS resistance and sus ceptibility. In this period, area under the diseaseprogress curve (AUDPC) of Hartz 6686 was the highest and that of PI 520733 was the lowest. SDS caused serious damage to the growth of soybean in all cultivars. Average reductions of growth rate of root fresh weight and dry weight were greater than those of plant tops. Duyu-kong showed less severe leaf symptoms than that of SDS suscetible cultivars; however, average growth rate of plants top and roots of this cultivar was less but not significantly different than those of SDS susceptible cultivars. In all cultivars, as severity of leaf symptoms increased, plant top weight decreased. Root rot symptoms were observed in all cultivars before leaf symptoms appeared. Average proportions of tap root reddish-brown discoloration of all cultivars was up to 75 % at 15 days after inoculati on; however there was no significant differenc between cultivars at each rating date. Appearances of leaf symptoms on leaves varied in each cultivar. SDS resistant cultivars had a significantly higher level of crinkling than susceptible cultivars and SDS susceptible cultivars had a significantly higher level of necrosis than resistant cultivars. Further study will be needed to identify the relationships between the physiological growth rate and SDS severities in soybeans.

Improvement of Transformation Efficiencies using Agrobacterium-Mediated Transformation of Korean Rice

Joon Hyeong Cho,Jang Yong Lee,Yong Wook Kim,Myoung Hoon Lee,Seong Ho Choi 韓國作物學會 2004 Korean journal of crop science Vol.49 No.1

A reproducible transformation system via optimized regeneration media for Korean rice cultivars was established using Agrobacterium tumefeciens LBA4404 (pSBM-PPGN; gusA and bar). Although japonica rice genotypes were easier to produce transgenic plants compared to Tongil type cultivars, transformation efficiencies were not always correlated with regeneration efficiencies of non-transgenic callus on the control medium. Regeneration efficiencies of Donganbyeo, Ilmibyeo, and Manchubyeo were over 50% in non-transgenic control, however, transformation efficiencies were significantly low when only sucrose was added to the media as a carbon source. However, the medium, MSRK5SS-Pr (or MSRK5SM-Pr), that contains 5~textrmmgL-1 kinetin, 0.5~textrmmgL-1 NAA, 2 % sucrose (or maltose), 3% sorbitol, and 500~textrmmgL-1 proline, was the most efficient not only for regeneration of non-transgenic callus but also for regeneration of transgenic callus in the presence of L-phosphinotricin (PPT). Average transformation efficiencies of 16 Korean rice cultivars were significantly enhanced by using the optimized medium from 1.5% to 5.8% in independent callus lines and from 2.9% to 19.4% in tromsgenic plants obained. Approximately 98.9% (876 out of 885) transgenic plants obtained on optimized media showed basta resistance. Stable integration, inheritance and expression of gusA and bar genes were continued by GUS assay and PCR and Southern analysis of the bar gene. With Pst1 digestion of genomic DNA of transgenic plants, one to five copies of T-DNA segment were observed; however, 76% (19 out of 25 transgenic plants) has low copy number of T-DNA. The transformants obtained from one callus line showed the same copy numbers with the same fractionized band patterns.

Improvement of Shoot Regeneration from Scutella-Derived Callus in Rice

Joon Hyeong Cho,Jang Yong Lee,Yong Wook Kim 韓國作物學會 2004 한국작물학회지 Vol.49 No.1

The optimized in vitro culture system was investigated for improvement of regeneration efficiencies by observing the responses of scutella-derived callus of Korean rice (Oryza sativa L.). Large variations of callus induction (43.9-93.9%) and shoot regeneration (0-88.7%) were observed among the rice cultivars depending on medium. However, shoot regeneration was significantly improved by selected utilization of basal medium, growth regulators, and carbon sources. N6 basal medium was more efficient for embryogenic callus induction than MS or LS basal medium, while MS was superior to N6 for shoot regeneration. The calli of highly regenerative cultivars grew faster and showed higher rates of green tissue formation (GT) and shoot regeneration (SR) and lower rate of callus browning (CB) than those of recalcitrant cultivars. Although a higher level of kinetin stimulated the GT and SR in highly regenerative cultivars, 10~textrmmgL-1 kinetin generally suppressed the GT and SR, while CB was accelerated compared to 2~textrmmgL-1 kinetin. Additional benefits of sorbitol combined with maltose (or sucrose) under 5~textrmmgL-1 kinetin were certainly confirmed on regeneration efficiencies compared to sucrose alone as carbon source and osmotic regulator. This combination showed high rate of GT and SR with multiple shoots while low rate of CB. With MSRK5SM-Pr medium (5~textrmmgL-1 kinetin, 3% sorbitol, 2% maltose, 500~textrmmgL-1 proline), the regeneration efficiencies of total 17 out of 24 cultivars were practically improved 160% on average compared to MSRK2S (2~textrmmgL-1 kinetin, 3% sucrose) control medium. Especially, the medium was most effective to the cultivars showing a medium level of regenerability such as Daesanbyeo and Dongjinbyeo and Suwon477, enhancing efficiencies more than 300-600% compared to MSRK2S medium.

Screening of Korean Rice Cultivars for their Shoot Regeneration Ability in vitro and the Effect of Maltose on Shoot Regeneration

Joon Hyeong Cho,Han Sun Hur,Jang Yong Lee 한국육종학회 2003 한국육종학회지 Vol.35 No.4

Regeneration ability of plant is very important for producing transgenic plants by genetic engineering. Fifty rice cultivars and lines were screened for their regeneration ability in vitro and an attempt was made to increase shoot regeneration by substitu