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활성형 Vitamin D₃ 전환능을 소유한 방선균에서 P-450 Hydroxylase유전자의 클로닝 전략
서주원,현창구,김정미,김승영,홍순광 명지대학교 자연과학연구소 1998 자연과학논문집 Vol.17 No.-
비타민 D₃는 포유동물이나 새의 간에서 25-hydroxyvitamin D₃[25(OH)D₃]로 전환되는데, 이 hydroxylation은 P-450 hydroxylase에 의해 촉매된다고 알려져있다. 본 연구에서는 활성형 비타민 D₃전환능을 소유한 방선균에서P-450 hydroxylase효소를 신속하게 분리가능한 PCR방법을 보고하고자 한다. Primer는 방선균에서 보고된 P-450 hydroxylase들 사이의 아미노산 서열의비교를 통해 산소결합부위와 heme-ligand pocket 주의에서 제작되었고,5종의 방선균에서 효과적으로 PCR증폭산물을 획득할 수 있었다. 증폭산물을 획득할 수 있었다.증폭 산물들의 아미노산서열을 분석한 결과 기존에 밝혀진 많은P-450 hydroxylase유전자들과 상동성을 보이고 있었으며, 특히 Streptomyces griseolus에서 분리되어 sulfonylurea 등의 제초제의 분해능력을 가진 SuaC, SubC 효소 등과 높은 아미노산 상동성을 지니고 있었다. 이러한 PCR전략은 방선균에서 유용한 물질의 대사에 관여하는 P-450 hydroxylase효소의 클로닝에 도움을 줄 것이라 사료된다 In mammals and birds, vitamin D₃is converted to 25-hydroxyvitamin D₃[25(OH)D₃] and then to 1α25-dihydroxyvitamin D₃[25(OH)₂D₃]in the liver and kideny, respectively these hydroxylating reactions are known to be catalyzed by P-450 hydroxylases. In this paper we report a PCR method which can be used for the repid amplification of DNA fragments for _450 hydroxylase from actinomycetes which can convert to active from of vitamin D₃. Primers were designed based on several regions sharing strong similarities in amino acid sequence of P-450 hydroxylases from a variety of actinomycetes, primarily in the regions of an oxygen binding site and a heme ligand pocket. These primers were used to amplify DNA fragments from five different actinomycetes. The deduced amino acid sequences of the isolated fragments revealed significant similarities to known P-450 hydroxylase including the product of the suaC or subC genes from Streptomyces griseolus that is capable of metabolizing a number of sulfonylurea herbicides and to the product of the P450sca2 from S. carbophilus that produces a specific HMG-CoA reductase inhibitor. This method will be helpful for the researchers in cloning the genes for P-450 hydroxylase involved in the biosynthesis of useful compounds.
HPLC를 이용한 고추의 품종별 Capsaicin 및 Dihydrocapsaicin 성분의 함량 분석
맹주양;이용구;이숙재;정진아;나성희;김창원;심혜진 순천향대학교 기초과학연구소 2010 순천향자연과학연구 논문집 Vol.16 No.2
Five kinds of Korean red pepper-pinkish, perfect, cucumber, blue, and ground cherry-were used, respectively, to analyze the contents of Capsaicin and Dihydrocapsaicin. The comparative analysis was performed separately in the seed removed red pepper and the seed included red pepper by HPLC. Among the seed removed pepper, the results for the content of Capsaicin were shown as follows: ground cherry(2204.0ppm) > pinkish(1324.9ppm) > blue(906.7ppm) > cucumber (77.2ppm) > perfect(12.9ppm), and the results for the content of Dihydrocapsaicin were shown as follows: ground cherry (1004.8ppm) > pinkish(248.3ppm) > blue(165.7ppm) > cucumber(13.9ppm) > perfect(0.0ppm). Among the seed included red pepper, the results for the content of Capsaicin were shown as follows: ground cherry(2190.lppm) > pinkish(1324.4ppm) > blue (919.9ppm) > cucumber(79.5ppm) > perfect(11.7ppm), and the results for the content of Dihydrocapsaicin were shown as follows: ground cherry(1017.4ppm) > pinkish(270.6ppm) > blue(189.0ppm) > cucumber(39.4ppm) > perfect(0.0ppm). Overall results suggest that in the contents of Capsaicin and Dihydrocapsaicin, the seed removed pepper and the seed included red pepper both were decreased in the order of ground cherry > pinkish > blue > cucumber > perfect, and this order is proportional to the strength of hot taste of red pepper.
편측성 하악과두증식증에서 수술방법에 의한 술후 골격구조의 대칭성에 관한 비교연구
홍광진,이원학,조윤주,이정구,민병진 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.2
In patients with unilateral condylar hyperplasia, increased length of the condylar neck causes deviation of the mandible to the opposite site, resulting in facial asymmetry, but the arch form and general contour of the displaced mandible is approximately symmetric. So, the unilateral condylectomy have been prefered surgical method among the surgeons. The purpose of this study is to analyze the postoperative results comparatively following two different surgical methods, condylectomy and ramus surgery, in unilateral condylar hyperplasia. Case of 6 patients with the unilateral condylar hyerplasia were analyzed in this study, 3 case of the condylectomy only, 3 cases of the ramus surgery, Clinical and radiographic evaluations were tried for comparison. In conclusion, comparative results between two groups did not show any significant difference,but we confirmed that unilateral condylectomy could be a excellent surgical methods in unilateral condylar hyperplasia thinking over the unique morphological characteristics of it.
박재용,이원구,유진식,김용대,한석영,맹주성 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-
An active micro-mixer, which was composed of an oscillating micro-stirrer in the microchannel to provide rapid, effective mixing at high flow rates was analyzed. The effects of molecular diffusion and disturbance by the stirrer were considered with regard to two types of mixer models: the simple straight microchannel and microchannel with an oscillating stirrer. Two types of mixer models were studied by analyzing mixing behaviors such as their interactionafter the stirrer. The mixing was calculated by Lattice Boltzmann methods using the D2Q9 model, In this study, the time-averaged mixing index formula was used to estimate the mixing performance of time-dependent flow. The mixing indices of the two models were compared. From the results, it was found that the mixer with an oscillating stirrer was much more enhanced and stabilized. Therefore, an approximate optimization of an active micro-mixer with an oscillating stirrer was performed using Kriging method with OLHD(Optima1 Latin Hypercube Design) in order to obtain a optimum solution. The design parameters were established as the frequency, the length and the angle of the stirrer, and the optimal values were determined to be 1.0346, 0.66D and ±45°, respectively. It was found that the mixing index of the optimal design increased by 88.72% compared with that of the original design.
박재용,김상락,이원구,유진식,김용대,한석영,맹주성 한국공작기계학회 2007 한국공작기계학회 추계학술대회논문집 Vol.2007 No.-
An active micro-mixer, which was composed of an oscillating micro-stirrer in the microchannel to provide rapid, effective mixing at high flow rates was analyzed. The effects of molecular diffusion and disturbance by the stirrer were considered with regard to two types of mixer models: the simple straight microchannel and microchannel with an oscillating stirrer. Two types of mixer models were studied by analyzing mixing behaviors such as their interactionafter the stirrer. The mixing was calculated by Lattice Boltzmann methods using the D2Q9 model. In this study, the time-averaged mixing index formula was used to estimate the mixing performance of time-dependent flow. The mixing indices of the two models were compared. From the results, it was found that the mixer with an oscillating stirrer was much more enhanced and stabilized. Therefore, an optimum design for a dynamic micro-mixer with an oscillating stirrer was performed using Kriging method in order to obtain a optimum solution. The design parameters were established as the frequency, the length and the angle of the stirrer, and the optimal values were determined to be 2.0932, 0.8D and ± 75°, respectively. It was found that the mixing index of the optimal design increased by 88.47% compared with that of the original design.
( Won Gu Kim ),( Tae Yong Kim ),( Tae Hyuk Kim ),( Hye Won Jang ),( Young Suk Jo ),( Young Joo Park ),( Sun Wook Kim ),( Won Bae Kim ),( Min Ho Shong ),( Do Joon Park ),( Jae Hoon Chung ),( Young Kee 대한내과학회 2014 The Korean Journal of Internal Medicine Vol.29 No.3
Background/Aims: Follicular thyroid carcinoma (FTC) and Hurthle cell carcinoma(HCC) of the thyroid are relatively uncommon thyroid malignancies in iodine-sufficient areas. In this study we evaluated the clinical behavior, prognosticfactors and treatment outcomes of FTC and HCC in Korea. Methods: This multicenter study included 483 patients with FTC and 80 patientswith HCC who underwent an initial surgery between 1995 and 2006 in one of thefour tertiary referral hospitals in Korea. We evaluated clinicopathological factorsassociated with distant metastases and recurrence during a median of 6 years offollow-up. Results: HCC patients were significantly older (49 years vs. 43 years; p < 0.001) andhad more lymphovascular invasions (22% vs. 14%; p = 0.03) compared with FTCpatients. Distant metastases were confirmed in 40 patients (8%) in the FTC groupand in two patients (3%) in the HCC group (p = 0.07). Distant metastases weresignificantly associated with older age, widely invasive cancer and extrathyroidalinvasion. Only 14 patients (3%) had recurrent disease and there was no significantdifference between FTC and HCC groups (p = 0.38). Recurrence was associatedwith larger tumor size and cervical lymph node metastasis. Conclusions: HCC patients were older and had more lymphovascular invasionsthan FTC patients. However, FTC and HCC patients had similar initial clinicopathologicalfeatures. Older age, wide invasiveness and extrathyroidal invasionwere independent risk factors for predicting distant metastases in FTC and HCCpatients.
주원구(Won-Gu Joo) 한국유체기계학회 1999 유체기계 연구개발 발표회 논문집 Vol.- No.-
The comming of high speed computers with large memory size in recent years has allowed the practical development of codes which solve the Reynolds-averaged NAvier-Stokes (RANS). equations in three dimensions. Such codes are already used by the large engine manufacturers for the advanced design of some engine components. Different computational fluid dynamics approaches and turbulence models exist, and it seems essential today to establish their degree of validity for application to typical configurations in turbomachinery. In 1993 the Turbomachinery Committee of the IGTI of ASME has issued an open invitation to predict the flow details of an isolated transonic fan rotor called as NASA ROTOR 37. This paper reports this test case.