Xanthones from the stems of <i>Cudrania tricuspidata</i> and their inhibitory effects on pancreatic lipase and fat accumulation

Jo, Yang Hee,Kim, Seon Beom,Ahn, Jong Hoon,Turk, Ayman,Kwon, Eun-Bin,Kim, Mun-Ock,Hwang, Bang Yeon,Lee, Mi Kyeong Elsevier 2019 Bioorganic chemistry Vol.92 No.-

<P><B>Abstract</B></P> <P>Nine new xanthones, cudracuspixanthones I - Q (<B>12</B>–<B>14</B>, <B>25</B>, <B>32</B>–<B>36</B>), and 30 known xanthones (<B>1</B>–<B>11</B>, <B>15</B>–<B>24</B>, <B>26</B>–<B>31</B>, <B>37</B>–<B>39</B>) were isolated from the stems of <I>Cudrania tricuspidata</I> (Moraceae). The structures of isolated compounds were established by using 1D and 2D NMR in combination with HR-TOF-MS. Xanthones from the stems of <I>C. tricuspidata</I> exerted pancreatic lipase inhibitory activity. In addition, cudracuspixanthone P (<B>35</B>), a new xanthone, reduced the fat accumulation in liver cells stimulated with fatty acids. Therefore, these compounds might be beneficial in the treatment of metabolic diseases.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Thirty nine xanthones were isolated from the stem of <I>Cudrania tricuspidata</I>. </LI> <LI> Nine xanthones, cudracuspixanthones I – Q, were previously undescribed. </LI> <LI> These xanthones showed inhibitory effects on pancreatic lipase and fat accumulation. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Isolation of Bacteria with Protease Activity from Cheonggukjang and Purification of Fibrinolytic Enzyme

Yeon Hee Choi(최연희),Jun Seung Lee(이준승),So Young Bae(배소영),Keun Jae Yang(양근재),Kyu Won Yeom(염규원),Dong Hyeok Jo(조동혁),Ock hwa Kang(강옥화),Hyung Suk Baik(백형석) 한국생명과학회 2013 생명과학회지 Vol.23 No.2

혈전용해효소를 생산하는 균주 분리를 위해서 우선 한국, 일본 등지에서 모은 21개의 청국장 시료를 준비하였고 총 268개의 균주를 분리하였다. 이 중에서 1% skim milk가 포함된 nutrient agar 배지에서 protease를 생산하는 bacteria를 분리하였고, 이 결과로 22개의 균주가 분리되었다. 균주들은 apiweb을 통해 생화학적 특성에 근거하여 동정하였다. 또한 세균동정을 위해 16S rRNA 염기서열 분석을 수행하였다. 분리된 대부분의 균주는 Bacillus subtilis와 Bacillus amyloliquefaciens였다. 혈전용해효소의 활성은 fibrin plate 방법에 의해 측정되었고 A2-14, A2-20, C1-05, C1-09, F2-01로 명명된 다섯 균주가 선택되었다. 이중에서 A2-20 균주는 강한 혈전용해 활성을 보였고 동정결과 Bacillus amyloliquefaciens에 가까웠다. A2-20 균주에 의해 생산되는 혈전용해효소는 균 상등액을 이용한 gel filtration과 ion exchange chromatography를 거쳐 부분정제 되었다. 부분 정제된 효소의 최적 pH는 7.0이었고, 최적 온도는 35℃였다. 정제된 단백질의 분석은 SDS-PAGE와 zymography로 이루어졌다. 이와 더불어 혈전용해효소의 유전자적 분석도 수행되었으며 A2-20 균주가 생산하는 혈전용해효소의 부분적인 염기서열과 유전적 상동성을 보이는 서열을 규명하였다. To isolate the fibrinolytic enzyme, 268 strains from 21 samples were morphologically isolated from Cheonggukjang collected from Korea and Japan. Among the 268 strains, protease-producing bacteria were isolated in nutrient agar medium including 1% skimmed milk. As a result of this, 22 strains were isolated. Apiweb site was used to identify these strains based on their biochemical properties. In addition, 16S rRNA sequencing was performed to identify the strain. Most of the identified strains were Bacillus subtilis and B. amyloliquefaciens. Fibrinolytic enzyme activity was measured with the fibrin plate method. Five strains were finally selected: A2-14, A2-20, C1-05, C1-09, and F2-01. Of those five strains, the A2-20 strain, which is close to B. amyloliquefaciens, showed the strongest fibrinolytic activity. The fibrinolytic enzyme produced by the A2-20 strain was partially purified from culture supernatant by gel filtration and ion exchange chromatography. The optimal pH and temperature values of the partially purified enzyme were 7.0 and 35°C, respectively. Purified protein analysis was carried out with SDS-PAGE and zymography. A genetic analysis was also conducted by PCR based on the consensus sequence of fibrinolytic enzyme. Corresponding genes with a partial sequence of the A2-20 strain were identified.

김치를 이용한 타블렛 조성물이 고지방식이로 유도된 흰쥐의 비만 억제에 미치는 효과

공연희(Yeon?Hee Kong),최홍식(Hong?Sik Cheigh),송영옥(Young?Ok Song),조연옥(Youn?Ock Jo),최상윤(Sang Yoon Choi) 한국식품영양과학회 2007 한국식품영양과학회지 Vol.36 No.12

동결건조된 김치분말을 주성분으로 하여 부형제로 glucose, 고춧가루, 식이섬유소 등을 혼합하여 제조된 김치 타블렛 제조용 조성물(KTC)의 항비만 효과를 알아보기 위하여 4주령의 SD계 수컷 흰쥐를 식이에 따라 정상대조군, 고지방식이군, 고지방 식이에 KTC 5%와 10% 첨가군의 4군으로 나누어 8주간 사육하고 체중, 피하지방, 혈당, 혈 중 지질농도, 간조직의 지질 농도와 HMG-CoA reductase 활성, 분변으로의 지질 배설능 등을 측정한 결과는 다음과 같다. 고지방 식이에 KTC 첨가량이 늘어날수록 흰쥐의 혈중 중성지질, 총콜레스테롤치를 농도 의존적으로 감소시켜 혈액내 지방의 축적을 저해하였고, 복부 피하지방의 양은 KTC 5%, 10% 첨가군이 고지방군에 비해 13.6%, 21.3% 감소시켰다(p<0.05). 또한 간과 심장, 고환의 중성지방, 콜레스테롤 농도는 KTC 첨가군이 고지방식이군에 비해 농도 의존적으로 감소하였고(p<0.05), HMG-CoA reductase 활성 및 혈당 농도를 고지방식이군에 비해 유의적으로 감소시켜 KTC가 흰쥐의 비만을 억제함을 확인할 수 있었다. Anti-obesity effects of kimchi tablet composition (KTC) were investigated with male Sprague-Dawley rats fed high-fat diet for 8 weeks. Fifty rats were classified to 4 groups: normal diet (ND), high fat diet (HFD), HFD+KTC 5% and HFD+KTC 10% for feeding experiments. This research showed that the final weight, weight gain, food efficiency ratio and organ weight were decreased by the addition of KTC compared to those of HFD group. Amount of subcutaneous fat of KTC groups were 13.6% and 21.3%, respectively. The plasma triglyceride concentration of KTC groups was lower than that of high fat diet group. Plasma cholesterol concentration of all four groups were not significantly different. HDL-cholesterol concentration of KTC groups was higher than those of high fat diet group. In addition, triglyceride and cholesterol concentrations in the liver and heart were reduced by the addition of KTC. Moreover, triglyceride concentration of fecal and hepatic HMG-CoA reductase activity were increased in KTC group. These results suggested that KTC might be useful for obesity control and good source of functional kimchi tablet.

김치를 이용한 타블렛 조성물이 고지방식이로 유도된 흰쥐의 비만 억제에 미치는 효과

공연희 ( Yeon Hee Kong ),최홍식 ( Hong Sik Cheigh ),송영옥 ( Young Ok Song ),조연옥 ( Youn Ock Jo ),최상윤 ( Sang Yoon Choi ) 부산대학교 김치연구소 2009 김치의 과학과 기술 Vol.12 No.-

Anti-obesity effects of kimchi tablet composition (KTC) were investigated with male Sprague-Dawley rats fed high-fat diet for 8 weeks. Fifty rats were classified to 4 groups: normal diet (ND), high fat diet (HFD), HFD + KTC 5% and HFD + KTC 10% for feeding experiments. This research showed that the final weight, weight gain, food efficiency ratio and organ weight were decreased by the addition of KTC compared to those of HFD group. Amount of subcutaneous fat of KTC groups were 13.6% and 21.3%, respectively. The plasma triglyceride concentration of KTC groups was lower than that of high fat diet group. Plasma cholesterol concentration of all four groups were not significantly different. HDL-cholesterol concentration of KTC groups was higher than those of high fat diet group. In addition, triglyceride and cholesterol concentrations in the liver and heart were reduced by the addition of KTC. Moreover, triglyceride concentration of fecal and hepatic HMG-CoA reductase activity were increased in KTC group. These results suggested that KTC might be useful for obesity control and good source of functional kimchi tablet.

Inhibitory Effects of 2-(4-Chlorophenyl)-1,3-selenazol-4-one on Lipopolysaccharide-induced Nitric Oxide Production in RAW 264.7 Cells

( Sang Yoon Choi ),( Yeon Ock Jo ),( Mamoru Koketsu ),( Hideharu Ishihara ),( Sung Hoon Kim ),( Sun Yeou Kim ) 한국응용생명화학회 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.4

In this study, the inhibitory effects of selenium-containing heterocyclic compound, selenazol derivatives on LPS-induced nitric oxide production in macrophage cells (RAW 264.7) were assessed. Five kinds of selenazol derivatives were shown to exhibit 5.4~41.7% of inhibitory effects at 10 μM. Among them, 2-(4-chlorophenyl)-1,3-selenazol-4-one generally evidenced a high degree of inhibitory activity with IC50=11.8 μM, and this compound also profoundly suppressed iNOS expression in LPS-treated RAW 264.7 cells. These results indicated that 2-(4-chlorophenyl)-1,3-selenazol-4-one might function as an anti-inflammatory agent via the inhibition of iNOS-mediated nitric oxide production.

The role of postoperative pelvic radiation in stage IV rectal cancer after resection of primary tumor

Lee, Joo Hwan,Jo, In Young,Lee, Jong Hoon,Yoon, Sei Chul,Kim, Yeon-Sil,Choi, Byung Ock,Kim, Jun-Gi,Oh, Seong Taek,Lee, Myeong A,Jang, Hong-Seok The Korean Society for Radiation Oncology 2012 Radiation Oncology Journal Vol.30 No.4

Purpose: To evaluate the effect of pelvic radiotherapy (RT) in patients with stage IV rectal cancer treated with resection of primary tumor with or without metastasectomy. Materials and Methods: Medical records of 112 patients with stage IV rectal cancer treated with resection of primary tumor between 1990 and 2011 were retrospectively reviewed. Fifty-nine patients received synchronous or staged metastasectomy whereas fifty-three patients did not. Twenty-six patients received pelvic radiotherapy. Results: Median overall survival (OS), locoregional recurrence-free survival (LRFS), and progression-free survival (PFS) of all patients was 27, 70, and 11 months, respectively. Pathologic T (pT), N (pN) classification and complete metastasectomy were statistically significant factors in OS (p = 0.040, 0.020, and 0.002, respectively). RT did not improve OS or LRFS. There were no significant factors in LRFS. pT and pN classification were also significant prognostic factors in PFS (p = 0.010 and p = 0.033, respectively). In the subgroup analysis, RT improved LRFS in patients with pT4 disease (p = 0.026). The locoregional failure rate of the RT group and the non-RT group were 23.1% and 33.7%, showing no difference in the failure pattern of both groups (p = 0.260). Conclusion: Postoperative pelvic RT did not improve LRFS of all metastatic rectal cancer patients; however, it can be recommended to patients with pT4 disease. A complete resection of metastatic masses should be performed if possible.

Inhibitory Effects of 2-(4-Chlorophenyl)-1, 3-selenazol-4-one on Lipopolysaccharide-induced Nitric Oxide Production in RAW 264.7 Cells

( Sang Yoon Choi ),( Yeon Ock Jo ),( Mamoru Koketsu ),( Hideharu Ishihara ),( Sung Hoon Kim ),( Sun Yeou Kim ) 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.4

Changes in chest X-ray findings in 1- and 2-month group after treatment initiation for suspected pulmonary tuberculosis

Jang Ho Lee,Ock-Hwa Kim,Yeon Joo Kim,Tae Sun Shim,Kyung-Wook Jo 대한내과학회 2020 The Korean Journal of Internal Medicine Vol.35 No.5

Background/Aims: Although re-evaluation of radiographic follow-up after 2 to 3 months of therapy is recommended for patients administered anti-tuberculosis medication owing to suspected pulmonary tuberculosis, reported findings are limited. Therefore, this study aimed to investigate changes in 1- and 2-month chest X-ray (CXR) findings after the treatment initiation and compared them according to the final diagnosis of tuberculosis or non-tuberculosis. Methods: Patients who started anti-tuberculosis medication for suspected pulmonary tuberculosis were selected at a tertiary referral hospital in South Korea between January 2012 and December 2015. Changes in the 1- and 2-month CXR findings were classified as improved, unchanged, and aggravated. Results: Among the 120 patients enrolled in the 1-month CXR group, 76 (63.3%) had the final diagnosis of tuberculosis. Comparison between the 1-month CXR changes and diagnosis showed that the final diagnosis was tuberculosis in 81.8% (45/55), 50.0% (26/52), and 38.5% (5/13) of patients whose 1-month CXR was improved, unchanged, and aggravated, respectively. In the 2-month CXR group, 167 patients were enrolled, and 139 (83.2%) of them were diagnosed with tuberculosis. Tuberculosis was the final diagnosis in 92.6% (100/108), 70.0% (35/50), and 44.4% (4/9) patients with improved, unchanged, and aggravated 2-month CXR findings, respectively. In patients with the final diagnosis of non-tuberculosis, nontuberculous mycobacteria and malignancy were the most common causes of improved and aggravated 1- and 2-month CXR findings, respectively. Conclusions: Two-month CXR findings were of limited value for deciding on whether to continue anti-tuberculosis treatment. One-month CXR findings could help determine the need for further work-up.

Inhibitory Effects of 2-(4-Chlorophenyl)-1,3-selenazol-4-one on Lipopolysaccharide-induced Nitric Oxide Production in RAW 264.7 Cells

Choi, Sang-Yoon,Jo, Yeon-Ock,Koketsu, Mamoru,Ishihara, Hideharu,Kim, Sung-Hoon,Kim, Sun-Yeou The Korean Society for Applied Biological Chemistr 2009 Applied Biological Chemistry (Appl Biol Chem) Vol.52 No.4

In this study, the inhibitory effects of selenium-containing heterocyclic compound, selenazol derivatives on LPS-induced nitric oxide production in macrophage cells (RAW 264.7) were assessed. Five kinds of selenazol derivatives were shown to exhibit $5.4{\sim}41.7%$ of inhibitory effects at $10{\mu}M$. Among them, 2-(4-chlorophenyl)-1,3-selenazol-4-one generally evidenced a high degree of inhibitory activity with $IC_{50}=11.8{\mu}M$, and this compound also profoundly suppressed iNOS expression in LPS-treated RAW 264.7 cells. These results indicated that 2-(4-chlorophenyl)-1,3-selenazol-4-one might function as an anti-inflammatory agent via the inhibition of iNOS-mediated nitric oxide production.

The role of postoperative pelvic radiation in stage IV rectal cancer after resection of primary tumor

Joo Hwan Lee,In Young Jo,Jong Hoon Lee,Sei Chul Yoon,Yeon-Sil Kim,Byung Ock Choi,Jun-Gi Kim,Seong Taek Oh,Myeong A Lee,Hong-Seok Jang 대한방사선종양학회 2012 Radiation Oncology Journal Vol.30 No.3

Purpose: To evaluate the effect of pelvic radiotherapy (RT) in patients with stage IV rectal cancer treated with resection of primary tumor with or without metastasectomy. Materials and Methods: Medical records of 112 patients with stage IV rectal cancer treated with resection of primary tumor between 1990 and 2011 were retrospectively reviewed. Fifty-nine patients received synchronous or staged metastasectomy whereas fifty-three patients did not. Twenty-six patients received pelvic radiotherapy. Results: Median overall survival (OS), locoregional recurrence-free survival (LRFS), and progression-free survival (PFS) of all patients was 27, 70, and 11 months, respectively. Pathologic T (pT), N (pN) classification and complete metastasectomy were statistically significant factors in OS (p = 0.040, 0.020, and 0.002, respectively). RT did not improve OS or LRFS. There were no significant factors in LRFS. pT and pN classification were also significant prognostic factors in PFS (p = 0.010 and p = 0.033, respectively). In the subgroup analysis, RT improved LRFS in patients with pT4 disease (p = 0.026). The locoregional failure rate of the RT group and the non-RT group were 23.1% and 33.7%, showing no difference in the failure pattern of both groups (p = 0.260). Conclusion: Postoperative pelvic RT did not improve LRFS of all metastatic rectal cancer patients; however, it can be recommended to patients with pT4 disease. A complete resection of metastatic masses should be performed if possible.