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고속액체크로마토그래피에 의한 나프록센 광학이성질체의 분리 및 품질관리
정수진,진전성,이은주,이은실,강종성 忠南大學校 生命科學硏究院 醫藥品開發硏究所 2006 藥學論文集 Vol.21 No.-
Enantiomeric separation of naproxen is necessary in drug evaluation processes as the pharmacological activity of naproxen resides mainly in the (S)-enantiomer, The enantioseparation of naproxen was carried out with three different stationary phases. The methods were validated with the terms of linearity, intraday and interday precision, accuracy, selectivity and recovery. Among the selected stationary phases, ChiroSil DMB column with hexane : t-butylmethylether : acetic acid (60:40:0.1, v/v/v) as mobile phase showed the best enantioseparation of the naproxen in the commercial preparations. The wavelength for detection was 270 nm and flow rate was 2.0 mL/min. The contents of (S)-naproxen in the commercial preparations were 92.7 - 98.3%, indicating that all tested preparations were satisfactory to current regulation. However, the contents of (R)-naproxen as impurity in the commercial preparations were varied from 0.08% to 1.08%.
Cytotoxic Constituents from Angelicae Sinensis RadixKi-Hwan Bae
Chen, Quan Cheng,Lee, Jong-Pill,Jin, Wen-Yi,Youn, Ui-Joung,Kim, Hong-Jin,Lee, Ik-Soo,Zhang, Xin-Feng,Song, Kyung-Sik,Seong, Yeon-Hee,Bae, Ki-Hwan 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5
Cytotoxic bioassay-guided fractionation of methanol extract of Angelicas Sinensis Radix led to the isolation of a new dimeric Z-ligustilide, named neodiligustilide (1), together with three known compounds, Z-ligustilide (2), 11(S),16(R)-dihydroxy-octadeca-9Z,17-dien-12, 14-diyn-1-yl acetate (3), and 3(R),8(S)-falcarindiol (4). Among them, 2 showed the strongest cytotoxicity against L1210 and K562 cell lines with IC$_{50}$ values of 2.27 ${\pm}$ 0.10 and 4.78 ${\pm}$ 0.18 ${\mu}$M, respectively, while 1 showed moderate cytotoxicity wiith IC$_{50}$ values of 5.45 ${\pm}$ 0.19 and 9.87 ${\pm}$ 0.14 ${\mu}$M. Two polyacetylenes, 3 and 4, showed cytotoxicity only against L1210 cell lire with IC$_{50}$ values of 2.60 ${\pm}}$ 0.90 and 2.87 ${\pm}$ 0.49 ${\mu}$M, respectively.
Aeromonas hydrophila cytosolic 5’-methylthioadenosine/S-adenosylhomocysteine nucleosidase MtaN-2
Jinli Chen,Fei Shang,Lulu Wang,Wei Liu,Yuanyuan Chen,Jing Lan,Liming Jin,Nam-Chul Ha,Chunshan Quan,Yongbin Xu 한국구조생물학회 2018 Biodesign Vol.6 No.3
5’-methylthioadenosine/S-adenosylhomocysteine nucleosidase (MTAN) plays a critical role in diverse pathways in bacterial cells such as biological methylation, polyamine biosynthesis, methionine recycling, and bacterial quorum sensing. It has been known that MtaN catalyzes the hydrolysis of N-ribosidic bond of adenosine-based substrates such as S-adenosyl-L-homocysteine (SAH), S-methyl-5’-thioadenosine (MTA) and 5’-deoxyadenosine (5’-DOA). In Aeromonas hydrophila, there are two MtnN subfamily proteins: MtaN-1, a periplasmic protein with an N-terminal signal peptide; and MtaN-2, a cytosolic protein. In this study, MtaN-2 from A. hydrophila was successfully expressed and purified using Ni-NTA affinity, Q anion-exchange, and gel-filtration chromatography. We first crystallized apo MtaN-2 but it diffracted to a low resolution of 5.1 Å. New crystals suitable for diffraction were obtained by adding 2 mM adenosine, a substrate analog of MtaN-2 during purification process and the crystals diffracted to the resolution of 2.0 Å. The crystals belong to the trigonal space group P31 or P32, with unit-cell parameters of a = b = 74.94 Å and c = 185.21 Å. The asymmetric unit contains four complexes of MtaN-2 with hydrolysis products of adenosine.
Liu Quan-quan,Zhang Chen,Zhou Jin-cheng,Dong Qian-jin,Huo Liang-xiao,Dong Hui 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.2
Ploidy diversity provides valuable scientific information, thus making the detection technique of ploidy important. However, traditional methods of cytological observation and flow cytometry are either laborious or expensive. We here report a simple and rapid, effective and economical quantitative PCR (qPCR) approach to determine the ploidy of a parasitoid species Trichogramma dendrolimi Matsumura, an economically important biocontrol agent. We applied a mitochondrial gene cytochrome oxidase (COI) and a nuclear gene forkhead to evaluate the mitochondrial number per nuclear copy in a thelytokous Wolbachia-infected strain of T. dendrolimi and its bisexual uninfected counterparts. The 2 −ΔCq values calculated from C q values which resulted from qPCR experiments were significantly larger in haploid males than that in diploid females. Haploid males possessed about 2.69 times mitochondrial number per nuclear copy as diploid females. Not a single significant difference was found between diploid females from thelytokous and bisexual strains. Based on the differences in relative mitochondrial content, we were allowed to distinguish between haploid males and diploid females. Moreover, the number of mitochondria significantly decreased with higher ploidy level but was not affected by Wolbachiainfection. Our study supplied an available tool to investigate the ploidy diversity in sex determination of T. dendrolimi and thelytokous manipulation of Wolbachia, which is the crucial step to further study their underlying mechanisms. This will in turn contribute to the biocontrol efficiency by enhancing the female production and hence the parasitism rate.
Assessment of Landslide Susceptibility using the PCA and ANFIS with Various Metaheuristic Algorithms
Zelu Chen,Hechun Quan,Ri Jin,Aifen Jin,Zhehao Lin,Guangri Jin,Guang-Zhu Jin 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.4
It is very important for the susceptibility assessment and disaster prediction of the region to effectively evaluate the landslide susceptibility. In this study, Particle Swarm Optimization (PSO), Artificial Bee Colony algorithm (ABC), Shuffled Frog Leaping Algorithm (SFLA) and Bat algorithm (BAT) are used to optimize Adaptive Neuro-Fuzzy Inference System (ANFIS) to evaluate the landslide susceptibility. 811 sample points were collected through remote sensing analysis and field investigation for susceptibility analysis. Fifteen landslide evaluation factors were quantified and normalized, and the Principal Component Analysis (PCA) method was used to compress them into 6 main factors. The accuracy analysis results of the area under the curve (AUC), Root Mean Square Error (RMSE) and Mean Absolute Error (MAE) evaluation models show that the AUC values of PSO, ABC, SFLA and BAT are 93.6%, 96.2%, 90.8% and 86.1%, respectively. Among them, the accuracy of ABC is the highest. This study effectively evaluates the landslide susceptibility through a new neural network hybrid method, which provides a theoretical basis for landslide disaster susceptibility management.
Aerodynamic shape optimized design for wind turbine blade using new airfoil series
Quan Wang,Jun Wang,Jin Chen,Song Luo,Jinfeng Sun 대한기계학회 2015 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.29 No.7
This paper introduces a new airfoil series to optimize the aerodynamic shape of wind turbine blades. It is verified that the CQU-A airfoilseries exhibits high aerodynamic performance by using the wind tunnel experimental data and RFOIL. The geometry of a 2 MWwind turbine blade with new airfoil families is designed preliminarily based on the shape of Tjaereborg 2 MW rotor. A multi-objectiveoptimized model combining the maximum power coefficient of the wind turbine with minimum area of the blade surface is proposed forthe pitch regulated wind turbine. An optimized code is developed based on the corrected blade element momentum (BEM) theory andparticle swarm optimization (PSO) algorithm. The optimization results show that, compared with that of the original rotor and the Tjaereborgrotor, not only the power coefficient and annual power production is improved, but also the area of the blade surface is reduced. The decreased area indicates that the mass of the optimized blades is reduced. It is beneficial for increasing the fatigue life and reducingcost of composite materials if the internal structure of the wind turbine blades is unchanged. Furthermore, the load of the blade root iseffectively controlled by using this alternative optimization program.
An Intelligent Management System for Evaluating Science Research Projects
Chen, Zhi-Yu,Chen, Shi-Quan,Wu, Jin-Pei Korean Institute of Industrial Engineers 2005 Industrial Engineeering & Management Systems Vol.4 No.1
Proposed in this paper is an intelligent management system for evaluating science research projects based on fuzzy neural networks with genetic algorithms. This system was planned, designed and tested employing theories and approaches of software engineering. This system was then applied to evaluate science research projects of the Natural Science Foundation of Guangdong Province, People’s Republic of China. The outcome / results shows the feasibility and validity of the system and its possible application to other intelligent management systems.
Cytotoxic Constituents from Angelicae Sinensis Radix
Quan Cheng Chen,JongPill Lee,WenYi Jin,윤의중,HongJin Kim,Ik Soo Lee,XinFeng Zhang,송경식,성연희,배기환 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.5
Cytotoxic bioassay-guided fractionation of methanol extract of Angelicae Sinensis Radix led to the isolation of a new dimeric Z-ligustilide, named neodiligustilide (1), together with three known compounds, Z-ligustilide (2), 11(S),16(R)-dihydroxy-octadeca-9Z,17-dien-12,14-diyn-1- yl acetate (3), and 3(R),8(S)-falcarindiol (4). Among them, 2 showed the strongest cytotoxicity against L1210 and K562 cell lines with IC50 values of 2.27 ± 0.10 and 4.78 ± 0.18 µM, respectively, while 1 showed moderate cytotoxicity with IC50 values of 5.45 ± 0.19 and 9.87 ± 0.14 µM. Two polyacetylenes, 3 and 4, showed cytotoxicity only against L1210 cell line with IC50 values of 2.60 ± 0.90 and 2.87 ± 0.49 µM, respectively.
Chen, Quan Cheng,Jeong, Su-Jin,Hwang, Gwi-Seo,Kim, Kyeong-Ho,Kang, Jong-Seong 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.4
A chiral mobile phase HPLC method is described for chiral separation and determination of chlorpheniramine (CP) enantiomers in various commercial preparations. Chromatographic separation was achieved on a conventional ODS column with a mixture of aqueous sodium phosphate (5 mM) containing 0.5 mM $carboxymethyl-{\beta}-cyclodextrin$, methanol and, triethylamine (73:25:2, v/v/v, pH 4.3) as the mobile phase. The flow rate of isocratic elution was 0,24 mL/min and peaks were detected at 224 nm. The method was applied to nine commercial CP preparations in six dosage forms and CP enantiomers were well separated without any disturbance of other ingredients or impurities present. The results showed that only one preparation was d-CP and the others were dl-CP preparations. The contents of all the preparations were found to be in the range of 97%-104% of labeled contents. This method was economical and convenient, affording sufficient accuracy, precision and reproducibility, as well as sensitivity and selectivity.