보문 : 국내에서 분리된 Verticillium dahliae의 유전적 유연관계 분석

( Shang Fei ),최유리 ( You Ri Choi ),송정영 ( Jeong Young Song ),김홍기 ( Hong Gi Kim ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.1

To provide basic information for Verticillium spp., molecular methods were applied to analyze genetic characteristics within Verticillium spp. including Verticillium dahliae, isolated from diseased plants in two regions of Korea. Five Korean isolates of V. dahliae causing Verticillium wilt on chrysanthemum were analyzed, together with six other Verticillium spp., using mitochondrial small subunit rRNA gene (rns) sequence and random-amplified polymorphic DNA (RAPD). In a phylogenetic tree based on rns region sequences, Korean V. dahliae isolates formed a single clade with foreign isolates, whereas the other Verticillium spp. formed separate groups. In addition to rns sequence analysis, a dendrogram based on RAPD fragment patterns also showed clustering of all V. dahliae isolates into one group, separate from the six different Verticillium spp., and the V. dahliae isolates formed three subgroups which corresponded to the regions of origin, Kumi, Busan city and Canada. This indicates that high genetic variation exists between regions, although the fungus was isolated from the same host plant, chrysanthemum. These results provide the foundation for the study of genetic diversity and relationships among V. dahliae isolates in Korea.

Crystal structure of the nicotinamidase/pyrazinamidase PncA from <i>Bacillus subtilis</i>

Shang, Fei,Chen, Jinli,Wang, Lulu,Jin, Liming,Zou, Linhai,Bu, Tingting,Dong, Yuesheng,Ha, Nam-Chul,Nam, Ki Hyun,Quan, Chunshan,Xu, Yongbin Elsevier 2018 Biochemical and biophysical research communication Vol.503 No.4

<P><B>Abstract</B></P> <P>The nicotinamidase/pyrazinamidase PncA is a member of a large family of hydrolase enzymes that catalyze the deamination of nicotinamide to nicotinic acid. PncA also functions as a pyrazinamidase in a wide variety of eubacteria and is an essential coenzyme in many cellular redox reactions in living systems. We report the crystal structure of substrate-free PncA from <I>Bacillus subtilis</I> (BsPncA) at 2.0 Å resolution to improve our understanding of the PncA family. The structure of BsPncA consists of an α/β domain and a subdomain. The subdomain of BsPncA has a different conformation than that of PncA enzymes from other organisms. The B-factor analysis revealed a rigid structure of the α/β domain, while the subdomain is highly flexible. Both dimers and tetramers were observed in BsPncA protein crystals, but only dimers were observed in solution. Our results provide useful information that will further enhance our understanding of the molecular functions of PncA family members.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Crystal structure of <I>B. Subtilis</I> PncA consists of an α/β domain and a subdomain. </LI> <LI> The metal-binding site of BsPncA is located at the bottom of a large cavity between the core and the subdomain. </LI> <LI> Zinc ion is pentahedrally coordinated within several highly conserved amino acid residues and one water molecule. </LI> <LI> Both dimers and tetramers were observed in BsPncA protein crystals, but only dimers were observed in solution. </LI> </UL> </P>

Bacillus amyloliquefaciens NAD+-dependent protein deacetylase: purification, crystallization and X-ray crystallographic analysis

Fei Shang,Jinli Chen,Lulu Wang,Yuanyuan Chen,Jing Lan,Wei Liu,Liming Jin,하남철,Chunshan Quan,Yongbin Xu 한국구조생물학회 2019 Biodesign Vol.7 No.1

Sirtuins are NAD+-dependent deacetylase that are broadly conserved throughout bacteria, archaea, and eukaryotes. Themembers of sirtuins are important in regulating diverse biological pathways, including gene silencing, DNA repair, genomestability, longevity, metabolism, and cell physiology. Sirtuin from Bacillus amyloliquefaciens (BaSrtN) is a particularlyinteresting bacterial Sir2 homologue. In this study, to further understand the function and mechanisms of this protein,BaSrtN was successfully expressed and purified using Ni-NTA affinity, Q anion-exchange, and gel-filtration chromatography. Purified BaSrtN was crystallized and diffracted to the resolution of 1.45 Å. The preliminary crystallographic analysissuggested that BaSrtN crystal belongs to the trigonal space group P31 or P32, with unit-cell parameters of a = b = 90.115and c = 86.306 Å. Size-exclusion chromatography suggested that BaSrtN prefer to exit as monomers in solution.

Soil fungal communities of montane natural secondary forest types in China

Fei Cheng,Xin Wei,Lin Hou,Zhengchun Shang,Xiaobang Peng,Peng Zhao,Zhaoxue Fei,Shuoxin Zhang 한국미생물학회 2015 The journal of microbiology Vol.53 No.6

Distinctive plant communities may provide specific physical and chemical properties with soils by specific litters and root exudates to exert effects on soil microorganisms. Past logging activities in the Qinling Mountains induced diverse natural secondary forest types (NSFTs). How these recovered NSFTs regulate patterns of soil microbial communities remain limited. In the study, we used terminal-restriction fragment length polymorphism (T-RFLP) to precisely determine forest type-specific soil fungal diversity and composition in five NSFTs. Our results indicated that NSFTs had significant impacts on the soil fungal communities. The most diverse fungal species were found in the Armand pine (Pinus armandi) and Chinese pine (Pinus tabulaeformis) forest soils, followed by sharptooth oak (Quercus aliena var. acuteserrata) and Chinese pine-sharptooth oak forest soils, the wilson spruce (Picea wilsonii) forests had the lowest soil fungal diversity. The analyses of community composition suggested that the fungal communities of Armand pine forest soils were similar to those of Chinese pine forest soils, while other communities prominently differed from each other. Stepwise multiple regression analysis revealed that soil silt, clay, pH, and ammonium nitrogen had intimate linkages with soil fungal diversity. Furthermore, the patterns of soil fungal communites were strongly governed by the specific soil environments of the tested NSFTs, as described by canonical correspondence analysis (CCA). Finally, our study showed that soil fungal communities may be mediated by NSFTs via specific soil edaphic status. Hence, such a comparable study may provide fundamental information for fungal diversity and community structure of natural forests and assist with better prediction and understanding how soil fungal composition and function alter with forest type transformation.

Structural and functional analyses of the lipase CinB from <i>Enterobacter asburiae</i>

Shang, Fei,Lan, Jing,Liu, Wei,Chen, Yuanyuan,Wang, Lulu,Zhao, Jing,Chen, Jinli,Gao, Peng,Ha, Nam-Chul,Quan, Chunshan,Nam, Ki Hyun,Xu, Yongbin Elsevier 2019 Biochemical and biophysical research communication Vol.519 No.2

<P><B>Abstract</B></P> <P>Lipases are widely present in various plants, animals and microorganisms, constituting a large category of enzymes. They have the ability to catalyze the cleavage of ester bonds. The lipase CinB from <I>Enterobacter asburiae</I> (<I>E. asburiae</I>) is an acetyl esterase. The primary amino acid sequence suggests that the EaCinB protein belongs to the α/β-hydrolase (ABH) superfamily of the esterase/lipase superfamily. However, its molecular functions have not yet been determined. Here, we report the crystal structure of <I>E. asburiae</I> CinB at a 1.45 Å resolution. EaCinB contains a signal peptide, cap domain and catalytic domain. The active site of EaCinB contains the catalytic triad (Ser180-His307-Asp277) on the catalytic domain. The oxyanion hole is composed of Gly106 and Gly107 within the conserved sequence motif HGGG (amino acid residues 106–109). The substrate is accessible between the α1 and α2 helices or the α1 helix and catalytic domain. Narrow substrate pockets are formed by the α2 helix of the cap domain. Site-directed mutagenesis showed that EaCinB-W208H exhibits a higher catalytic ability than EaCinB-WT by approximately nine times. Our results provide insight into the molecular function of EaCinB.</P> <P><B>Highlights</B></P> <P> <UL> <LI> EaCinB structure contains a signal peptide, cap domain and catalytic domain. </LI> <LI> The active site of EaCinB contains the catalytic triad (Ser180-His307-Asp277). </LI> <LI> The oxyanion hole is composed of Gly106 and Gly107. </LI> <LI> Substrate-binding mode of EaCinB are proposed. </LI> <LI> Site-directed mutagenesis showed that EaCinB-W208H exhibits a higher catalytic ability than EaCinB-WT. </LI> </UL> </P>

Analyzing Chinese Online P2P Financial Product Purchase Decisions Utilizing the Framing Effect 7)

Yu Fei Shang,김순홍 한국유통과학회 2015 유통과학연구 Vol.13 No.10

Purpose – This study examines Chinese P2P investment decision processes from a behavioral economics approach. Research design, data, and methodology – We analyze the online P2P investment product purchase decisions of 241 respondents in China, March 2015 to May 2015. T-tests were conducted to determine whether the framing effect influenced investor investment preferences. The Association Rule was used to identify the framing effect of respondent demographic characteristics on joint decisions regarding stable or risky investment products. Results – There are significant differences between the two groups (positive framing and negative framing) and their product- choosing behavior. In the positive framing group, female investors, young investors, investors with non-financial occupations and with limited or no experience, preferred stable P2P investment products. In contrast, in the negative framing group, investors with extensive investment experience preferred risky investment products. Conclusions – The framing effect influences investor choices in online P2P investment products. It is necessary to implement comprehensive supervision and full information disclosure regarding P2P investment products. P2P investment websites can also adopt different marketing strategies according to investor gender and age.

Hsp90 Inhibitor Geldanamycin Enhances the Antitumor Efficacy of Enediyne Lidamycin in Association with Reduced DNA Damage Repair

Han, Fei-Fei,Li, Liang,Shang, Bo-Yang,Shao, Rong-Guang,Zhen, Yong-Su Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Inhibition of heat shock protein 90 (Hsp90) leads to inappropriate processing of proteins involved in DNA damage repair pathways after DNA damage and may enhance tumor cell radio- and chemotherapy sensitivity. To investigate the potentiation of antitumor efficacy of lidamycin (LDM), an enediyne agent by the Hsp90 inhibitorgeldanamycin (GDM), and possible mechanisms, we have determined effects on ovarian cancer SKOV-3, hepatoma Bel-7402 and HepG2 cells by MTT assay, apoptosis assay, and cell cycle analysis. DNA damage was investigated with H2AX C-terminal phosphorylation (${\gamma}H2AX$) assays. We found that GDM synergistically sensitized SKOV-3 and Bel-7402 cells to the enediyne LDM, and this was accompanied by increased apoptosis. GDM pretreatment resulted in a greater LDM-induced DNA damage and reduced DNA repair as compared with LDM alone. However, in HepG2 cells GDM did not show significant sensitizing effects both in MTT assay and in DNA damage repair. Abrogation of LDM-induced $G_2/M$ arrest by GDM was found in SKOV-3 but not in HepG2 cells. Furthermore, the expression of ATM, related to DNA damage repair responses, was also decreased by GDM in SKOV-3 and Bel-7402 cells but not in HepG2 cells. These results demonstrate that Hsp90 inhibitors may potentiate the antitumor efficacy of LDM, possibly by reducing the repair of LDM-induced DNA damage.

A Study on On-line P2P Financial Product Purchase Decision of Chinese By Framing Effect

Yu Fei Shang,Na Zhang 한국유통과학회 2015 KODISA ICBE (International Conference on Business Vol.2015 No.-

Chinese P2P on-line loan financing product's rapid growth : P2P loan scale of 6 billion yuan (1 trillion 80 billion won) in 2012 has reached 83 billion yuan (14 trillion 940 billion won) until 2014. Thousands of on-line P2P companies have appeared. P2P is the abbreviation of peer to peer, which is a financing service that intermediates investment and loan between individuals. Lending club, listed with market capitalization about 9 trillion won in New York Stock Exchange in the end of last year, is the representative example, which is a field of Fin Tech business which is getting the limelight in the world. P2P loan product investment interest rate is quite higher than bank deposit investment interest, however lots of investment products have high risk. However, there are not sufficient research on this new financing exchange yet. Particularly, there's little research on P2P investment intention decision process in behavioral economics approach.

Effect of Experiential Value on Customer Satisfaction and e-WOM under O2O Commerce

Yu-Fei Shang(상옥비),Yao Chen(진요),Hong-Seop Kim(김홍섭) 한국유통과학회 2017 유통과학연구 Vol.15 No.8

Purpose - The online-to-offline (O2O) business model has brought considerable changes to the traditional Chinese business model. The main difference between O2O and pure online consumption is that O2O offers a richer experience and word-of-mouth. it is easier to trigger online word-of-mouth. However, few scholars have been concerned about the impact of experiential value on customer satisfaction and online word-of-mouth (e-WOM) in the study of O2O. This study takes the O2O business model in China’s catering industry as its research object and uses structural equation modelling to analyze the impact of online and offline experiential values on customer satisfaction and e-WOM. Research design, data, and methodology - According to previous researches, consumer experiential value is mainly divided into return on investment (economy and efficiency), service excellence, playfulness and aesthetics. According to the characteristics of O2O in China’s catering industry, this study divides the online experience value into efficiency and economy (return on investment). The offline part is divided into return on investment (economy and efficiency), service excellence, playfulness and aesthetics. Using a web-based survey, we collected 303 valid samples. Structural equation modelling was used to create the research model. Results - The results show that efficiency (online) and service excellence (offline) have a significant effect on customer satisfaction. Economics (online) and playfulness (offline) have a positive impact on customers’ e-WOM. In addition, the higher the customer satisfaction, the greater the positive impact on the spread by word of mouth. However, aesthetic(offline) and return on investment(offline) have no significant impact to customer satisfaction and e-WOM. Conclusions - The study findings show that the key to boost customer satisfaction in the catering industry is to improve product quality and service. Although traditional competitive strategies such

Two-phase aqueous systems of cetyltrimethylammonium bromide/sodium dodecyl sulfate with and without polyethylene glycol

Fei Wang,Yazhuo Shang,Ting Chen,Honglai Liu 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.3

Aqueous two-phase systems of cetyltrimethylammonium bromide and sodium dodecyl sulfate mixtures without and with polyethylene glycol (PEG) added have been studied at 313.15 K. The results have shown that PEG has a strong effect on the phase diagram of ATPS and microstructures of surfactants aggregates. The addition of PEG leads to the formation of bigger surfactant aggregates, which can be attributed to both the screening effect and the connection effect of PEG. All ATPS, especially those with PEG added, may have potential applications in separation and purification of biomaterials.