Lactobacillus casei AHU 1055 및 Lactobacillus acidophilus JAM 1043의 凍結乾燥와 生存率에 關한 硏究

金昌漢,金明浩,朴芝賢,李明燮,朴相瑨 건국대학교 동물자원연구센터 1992 動物資源硏究誌 Vol.17 No.-

In order to find the most suitable conditions of freeze-drying for the long term preservation of lacto-bacillus casei AHU 1055 and Lactobacillus acidophilus JAM 1043, the growth phase, the cryoprotection medium and inoculum size have been studied. The results obtained in this study are summerized as follows : 1. L. casei AHU 1055 and L.acidophilus JAM 1043 reached to stationary phase after incubating at 37˚C for 16hrs and 12hrs in MRS broth, respectively. 2. The highest viability of each strains was obtained in 11% skim-milk for L. casei AHU 1055 and 9% skim-milk for L. acidophilus JAM 1043 as basic cryoprotection medium. 3. The complex medium contained 11% skim milk powder, 2.5% arginine and 3% glucose was the best suspending midium for freeze-drying of L. casei AHU 1055, and the medium contained 9% skim milk powder, 7% glycerol, 9% lactose, 0.5% arginine was the best suspending medium for freeze-drying of L. acidophilus JAM 1043. 4. The strong positive correlation was found between inoculum size and the survival rate in both strains. 5. When the tested strains were cultured and freeze-dried under the best optimal conditions, the survival rates of freeze- dried L. casei AHU 1055 and L. acidophilus JAM 1043 were 85.8% and 84.0%, respectively.

자돈에 있어 게르마늄 용질액의 급여가 성장 및 혈액학적 변화에 미치는 영향

홍종욱,권오석,민병준,조진호,진영걸,손경승,강종옥,김인호 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.4

본 연구의 목적은 이산화게르마늄을 함유한 게르마늄 용질액을 자돈에 급여하였을 때 성장 및 혈액학적 변화에 미치는 영향을 조사하기 위하여 실시하였다. 3원 교잡종 자돈 60두 (평균체중 11.22±0.10㎏)를 공시하였다. 시험설계는 옥수수-대두박 위주의 대조구(CON)와, 대조구 사료내 게르마늄 용질액을 0.5 ppm 첨가한 구(GC0.5) 및 대조구 사료내 게르마늄 용질액을 1.0 ppm 첨가한 구(GC1.0)로 3개 처리를 하였다. 0-10일간 사양시험기간동안, 일당증체량에 있어서 GC0.5 처리구가 약간 높게 평가되었다(quadratic effect, P<0.02). 그러나 전체 시험기간동안, 일당증체량, 일당사료섭취량 및 사료효율에 있어서 처리구간에 유의적인 차이는 보이지 않았다. 영양소 소화율에 있어서는 GC0.5 처리구가 조회분(linear effect, P<0.01; quadratic effect, P<0.04), 칼슘(linear effect, P<0.01; quadratic effect, P<0.01), 인 (linear effect, P<0.02; quadratic effect, P<0.04) 소화율이 유의적으로 높게 평가되었다. 혈액내 neutrophil 농도에 있어서는 게르마늄 용질액의 첨가 수준이 증가함에 따라 유의적으로 증가하는 경향을 나타내었다(quadratic effect, P<0.02). 결론적으로, 자돈 사료내 게르마늄 용질액의 급여가 성장 및 혈액학적 수치에는 영향을 미치지 못하는 것으로 사료된다. This study was conducted to investigate the effects of dietary germanium colloid on the growth performance and hematological values in nursery pigs. Sixty pigs (11.22(0.10 ㎏ average initial body weight) were used in a 20 d growth assay. Dietary treatments included 1) CON (basal diet), 2) GC0.5 (basal diet+0.5 ppm germanium colloid) and 3) GC1.0 (basal diet+1.0 ppm germanium colloid). For d 0 to 10, pigs fed GC0.5 diet grew faster than pigs fed CON and GC1.0 diets (Quadratic effect, P<0.02). However, through the entire experimental period, no statistical differences were found for aver-age daily gain, average daily feed intake and gain/feed. Apparent digestibilities of crude ash (Linear effect, P<0.01; Quadratic effect, P<0.04), calcium (Linear effect, P<0.01; Quadratic effect, P<0.01) and phosphorus (Linear effect, P<0.02; Quadratic effect, P<0.04) in pigs fed GC0.5 diet were greater than for pigs fed CON and GC1.0. Neutrophil concentration in blood increased as the concentration of germanium colloid in the diets was increased with significant difference (Quadratic effect, P<0.02). In conclusion, growth performance and hematological values were not influenced by dietary germanium colloid.

L-carnitine treatment attenuates renal tubulointerstitial fibrosis induced by unilateral ureteral obstruction

( Hai Yan Zhao ),( Hui Ying Li ),( Jian Jin ),( Ji Zhe Jin ),( Long Ye Zhang ),( Mei Ying Xuan ),( Xue Mei Jin ),( Yu Ji Jiang ),( Hai Lan Zheng ),( Ying Shun Jin ),( Yong Jie Jin ),( Bum Soon Choi ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.0

Background/Aims: Accumulating evidence indicates that L-carnitine (LC) protects against multiorgan damage through its antioxidant properties and preservation of the mitochondria. Little information is available about the effects of LC on renal fibrosis. This study examined whether LC treatment would provide renoprotection in a rat model of unilateral ureteral obstruction (UUO) and in vitro. Methods: Sprague-Dawley rats that underwent UUO were treated daily with LC for 7 or 14 days. The influence of LC on renal injury caused by UUO was evaluated by histopathology, and analysis of gene expression, oxidative stress, mitochondrial function, programmed cell death, and phosphatidylinositol 3-kinase (PI3K)/ AKT/forkhead box protein O 1a (FoxO1a) signaling. In addition, H₂O₂-exposed human kidney cells (HK-2) were treated with LC. Results: LC treatment inhibited expression of proinflammatory and profibrotic cytokines, and was followed by a significant attenuation of tubulointerstitial inflammation and fibrosis. The increased oxidative stress caused by UUO was associated with mitochondrial dysfunction and excessive apoptosis and autophagy via PI3K/AKT/FoxO1a-dependent signaling, and this was abrogated by administration of LC. In H₂O₂-exposed HK-2 cells, LC decreased intracellular production of reactive oxygen species, and suppressed expression of profibrotic cytokines and reduced the number of apoptotic cells. Conclusions: LC protects against the progression of tubulointerstitial fibrosis in an obstructed kidney.

Defect Dominated Hierarchical Ti-Metal-Organic Frameworks via a Linker Competitive Coordination Strategy for Toluene Removal

( Jin Jie ),( Ma Zhongyuan ),박종혁 한국공업화학회 2021 한국공업화학회 연구논문 초록집 Vol.2021 No.0

Microstructure Distribution and Grain Coarsening Model of GCr15 Steel in the Laser Surface Treatment

Jin‑shan Chen,Zhen‑xing Li,Ya‑jie Chu,Jie Chen, Xin‑jun Shen 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.10

The GCr15 steel was subjected to laser surface treatment using a diode laser with rectangular spot. Based on the simulatedtemperature field, the microstructure distribution of the laser hardened layer was analyzed, and an empirical equation wasproposed to predict the peak temperature at different depths. The results indicated that with the decrease of depth, the peaktemperature increased exponentially, and the peak temperature at different depths was approximately linear with the powerdensity and the reciprocal of square root of scanning speed. High peak temperature led to the coarsening of grain and thesignificant dissolution of cementite near the surface of hardened layer. At the deeper position of hardened layer, the prioraustenite grain size decreased slightly due to high nucleation rate. The cementite dissolution and coarsening occurred simultaneouslynear the surface, and the cementite dissolution process was postponed due to the significant enrichment of Cr nearthe interface of austenite and cementite. Additionally, an empirical model about grain coarsening was established, which canbe used to predict the prior austenite grain size near the surface during the laser surface treatment.

PIV experimental study on flow structure and dynamics of square stirred tank using modal decomposition

Jie Jin,Ying Fan 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.5

Stirred mixing is one of the important unit operations in the chemical, petroleum, pharmaceutical and food industries. The mixing of liquids is achieved by a rotating shear flow field formed by a periodic jet flow from the impeller. In this work, we investigated the flow structure in a square stirred tank without baffles and with a Rushton impeller (RT) using particle image velocimetry (PIV) technique. The instantaneous flow fields were obtained as a function of various rotations per minute (rpm) for the impeller (N=120, 150, 180, 210 and 240 rpm), while phase-resolved velocity information was obtained for N=150 rpm. The proper orthogonal decomposition (POD) and dynamic mode decomposition (DMD) methods were applied to analyze the velocity fields, flow structure and dynamic information in the absence of impeller area. As demonstrated by the results, there is a wide range of spatial and temporal scales throughout the process. The high energy parts exist in two kinds of structures except for the average fluid flow. The instability phenomenon results from the cyclic shear flow and the trailing vortices structure caused by the periodic jet near the blade passage frequency. As the Reynolds number is on the rise, the periodic flow increases, the random turbulence is reduced, and the flow tends to the ultimate stable state. The square section acts like baffles to change the direction of the fluid circumferential velocity while increasing the radial and tangential flow, which is conducive to mixing. This study provides a basis for understanding the flow structure and unsteady characteristics in a square stirred mixing tank.

Variation and haplotypes of NaCl salinity on rice at germination stage based on Genome-wide association study

Jie Yu,Tae-Sung Kim,Yong-Jin Park 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07

Salt toxicity is the major factor limiting crop productivity in saline soils. Rice is an important staple food crop of nearly half of the world population and is well known to be a salt sensitive crop. The completion and enhanced annotations of rice genome sequence has provided the opportunity to study functional genomics of rice. With the rapid development of the biotechnology techniques, we can use more accurate and reliable methods to study the mechanism and function in different stress conditions. In present study, 295 rice accessions of diverse origin were re-sequenced and used for genome-wide association study (GWAS) with several germination-related traits, including germination percentage (GP), germination energy (GE), germination rate (GR), germination index (GI), salt tolerance index (STI) in salt tolerant germination stage. Phenotyping of the rice accessions were carried out at 200mM NaCl to screen salt tolerance levels. GWAS was applied to detect the associated genes related to salt tolerance in rice germination stage. Variations and haplotypes of the associated genes were detected and correlation between the phenotypes and genotypes were validated using qRT-PCR.

miRNA-218 Inhibits Osteosarcoma Cell Migration and Invasion by Down-regulating of TIAM1, MMP2 and MMP9

Jin, Jie,Cai, Lin,Liu, Zhi-Ming,Zhou, Xue-Song Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Deregulated miRNAs participate in osteosarcoma genesis. In this study, the expression of miRNA-218 in human osteosarcomas, adjacent normal tissues and Saos-2 human osteosarcoma cells was first assessed. Then the precise role of miRNA-218 in osteosarcoma cells was investigated. Upon transfection with a miR-218 expression vector, the proliferation of Saos-2 human osteosarcoma cells determined using the ATPlite assay was significantly suppressed, whilw migration of Saos-2 cells detected by wound healing and invasion determined using transwells were dramatically inhibited. Potential target genes of miR-218 were predicted and T-cell lymphoma invasion and metastasis 1 (TIAM1) and matrix metalloproteinase 2 (MMP2) and 9 (MMP9) were identified. This was confirmed by western blotting, which showed that miR-218 expression inhibited TIAM1, MMP2 and MMP9 protein expression. Collectively, these data suggest that miR-218 acts as a tumor suppressor in osteosarcomas by down-regulating TIAM1, MMP2 and MMP9 expression.

V-I Curves of p-ZnO:Al/n-ZnO:Al Junction Fabricated by RF Magnetron Sputtering

Jin, Hu-Jie,Jeong, Yun-Hwan,Park, Choon-Bae The Korean Institute of Electrical and Electronic 2008 전기전자재료학회논문지 Vol.21 No.6

Al-doped p-type ZnO films were fabricated on n-Si (100) and homo-buffer layers in pure oxygen at $450^{\circ}C$ of by RF magnetron sputtering. Target was ZnO ceramic mixed with 2 wt% $Al_2O_3$. XRD spectra show that the Al-doped ZnO thin films have ZnO crystal structure and homo-buffer layers are beneficial to Al-doped ZnO films to grow along c-axis. Hall Effect experiments with Van der Pauw configuration show that p-type carrier concentrations are ranged from $1.66{\times}10^{16}$ to $4.04{\times}10^{18}\;cm^{-3}$, mobilities from 0.194 to $2.3\;cm^2V^{-1}s^{-1}$ and resistivities from 7.97 to $18.4\;{\Omega}cm$. p-type sample has density of $5.40\;cm^{-3}$ which is smaller than theoretically calculated value of $5.67\;cm^{-3}$. XPS spectra show that Ols has O-O and Zn-O structures and Al2p has only Al-O structure. P-ZnO:Al/n-ZnO:Al junctions were fabricated by magnetron sputtering. V-I curves show that the p-n junctions have rectifying characteristics.

Defect Analysis via Photoluminescence of p-type ZnO:N Thin Film fabricated by RF Magnetron Sputtering

Jin, Hu-Jie,So, Soon-Jin,Park, Choon-Bae The Korean Institute of Electrical and Electronic 2007 전기전자재료학회논문지 Vol.20 No.3

ZnO is a promising material to make high efficient ultraviolet(UV) or blue light emitting diodes(LEDs) because of its large binding energy and energy bandgap. In this study, we prepared ZnO thin films with p-type conductivity on silicon(100) substrates by RF magnetron sputtering in the mixture of $N_2$ and $O_2$. The process was accompanied by low pressure in-situ annealing in $O_2$ at $600^{\circ}C$ and $800^{\circ}C$ respectively. Hall effect in Van der Pauw configuration showed that the N-doped ZnO film annealed at $800^{\circ}C$ has p-type conductivity. Photoluminescence(PL) spectrum of the film annealed at $800^{\circ}C$ showed UV emission related to exciton and bound to donor-acceptor pair(DAP) as well as visible emission related to many intrinsic defects.