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Effect of Chlorella Diet Supplementation on Blood and Urine Cadmium Levels in Cadmium Poisoned Rats
김용호,--,--,--,--,-- THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.3
This study was conducted to investigate the influence of chlorella diet supplementation. Blood accumulation and urine excretion levels were measured after the Sprague-Dawley (SD) rats were fed on a chlorella diet supplementation mixed with 40 ppm of CdCl₂. Four groups tested for blood accumulation and urine excretion levels. All four groups fed on a basic diet with a cadmium mixture. The diet for the first group contained only basic diet and the cadmium added to the drinking water. The diet for the three other groups contained cadmium to the drinking water, and 1%, 5% and 10% of chlorella added to the basic diet. A concentration of cadmium for the first group showed a 3.2±0.4 ㎍/l blood accumulatior. level and 41.5±32.9 ㎍/l urinary excretion level, and the second group, which was fed on the basic diet with 1% of chlorella added and cadmium to the drinking water showed a 1.5±0.6 ㎍/l blood level and only 14.1±1.6 ㎍/l urinary excretion level. The other two groups, which were fed on 5% and 10% of chlorella concentration and cadmium to the drinking water did not exhibit any notable effects greater than the group fed on 1% concentration of chlorella. The results suggest that the blood accumulation and urinary excretion of Cadmium are influenced by the chlorella diet supplementation from the concentration of 1% of the basic diet.
기립자세에서 발위치가 무릎 폄근의 등척성수축 근전도 활성도에 미치는 영향
김성중,권오윤,조상현,황지혜 한국전문물리치료학회 2001 한국전문물리치료학회지 Vol.8 No.2
This study was designed to identify the effects of foot position on electromyographic (EMG) activity of the quadriceps femoris during maximum voluntary contraction (MVC) in standing. Twenty young adults who had not experienced any knee injuries were recruited. Their Q-angles were within a normal range. They were asked to stand in five different foot positions (40˚externally rotated, 30˚ internally rotated, neutral, 20˚ plantarflexed, and 10˚dorsiflexed foot position). The EMG activities of the vastus lateralis (VL), rectum femoris (RF), and vastus medialis oblique (VMO) were recorded in standing by surface electrodes and normalized by MVC EMG values derived from manual muscle test. The normalized EMG activity levels (%MVC EMG) of muscles in the five foot positions were compared using repeated measures ANOVA. The EMG activity levels of the VL, RF, and VMO were the highest when foot was externally rotated. The EMG activity levels of the VL and RF were significantly different among the foot positions (p<.05). However, EMG activity levels of the VL, RF, VMO, and VMO/VL ratio did not show significant differences in each foot position (p> .05). The results suggest that the quadriceps femoris may be effectively activated by performing MVC a: an externally rotated foot position. Therefore, the externally rotated foot position can be considered as an effective foot position for quadriceps femoris strengthening exercise. Further studies are needed to identify whether there are differences in the effects of foot position on muscle strength after MVC exercise of quadriceps femoris in standing.
색상 측정 기기를 이용한 복합레진 적층 수복과 단일 수복의 색상 비교 분석
송영상,김자현,이빈나,장지현,장훈상,황윤찬,오원만,황인남 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.2
Objectives: This study analyzed the difference in color caused by different thickness in enamel layer of composite resins when applied with single and layering placement technique, and evaluated if the results agreed with the shade guide from the manufacturers to verify reliability of the color matching process of the manufacturers. Materials and Methods: For single composite resin samples, 6 mm diameter and 4 mm thickness cylindrical samples were fabricated using Ceram-X mono (DENTSPLY DeTrey) and CIE L*a*b* values were measured with spectrophotometer. Same process was done for layering compositie resin samples, making 3 dentinal shade samples, 4 mm thickness, for each shade using Ceram-X duo (DENTSPLY DeTrey) and enamel shade resins were layered in 2 mm thickness and CIE L*a*b* values were measured. These samples were ground to 0.2 mm thickness each time, and CIE L*a*b* values were measured to 1 mm thickness of enamel shade resin. Results: Color difference (△E*) between single and layering composite resin was 1.37 minimum and 10.53 maximum when layering thicknesses were between 1 mm and 2 mm and 6 out of 10 same shade groups suggested by manufacturer showed remarkable color difference at anythickness (△E* > 3.3). Conclusion: When using Ceram-X mono and duo for composite resin restoration, following the manufacturer's instructions for chossing the shade is not appropriate, and more accurate information for Ceram-X duo is needed on the variation and expression of the shades depending on the thickness of the enamel. (Restor Dent Endod 2012;37(2):84-89)
RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향
박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-
TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.
냉수 가글링과 젖은 거즈를 이용한 구강간호가 비강수술 환자의 갈증 및 구강상태에 미치는 효과 비교
이인선,심명숙,나은희,강재연,김지혜,황민영,윤계숙,성영희 병원간호사회 2006 임상간호연구 Vol.11 No.2
Purpose: This study researched the comparison of oral care methods between the wet gauze and the water gargling for nasal surgery patients. It executed to find out more effective method to relieve thirst. Method: We divided two groups; the wet gauze and the cold water garaling. We assessed patient's oral cavity state and sebjective oral dryness for three times; firstly before the intervention, secondly 15 minutes after 1st intervention and thirdly 15 minutes afrer 2nd intervention. Result: As the intervention, there was no difference on oral condition subhectvely, but there was a difference on thirsty reduction objectively. As the aral dryness has relieved through both methods. It showed the repeated gargling helps more to relieve oral dryness effectively than the wet gauge. At PACU, post nasal surgery patient's oral dryness was decreased with gauze more than two times, patient's oral dryness was relieved. Whereas the dryness was relieved after applying the cold water gargling for just one time. Conclusion: The water gargling is more effective intervention than the wet gauze. Especially, oral dryness was improved in the section of objetive oral assessment index.