RFID 사용요인이 신뢰, 지각된 위험, 모바일RFID환경 하에서 사용의도에 미치는 영향 -약국RFID 시스템을 중심으로-

최재영 ( Jae Young Choi1 ),차재빈 ( Jae Bin Cha1 ),김상만 ( Sang Man Kim ) 경희대학교 경영연구원 2013 의료경영학연구 Vol.7 No.1

This study examined the influence of various factors of RFID use in mobile RFID environment, focusing on pharmacies` RFID systems. The purpose of the study was to verify the possibility of a more widespread use of RFID and mobile RFID in medical industry by exploring what impact RIFD use factors have on the intention to use in RFID environment. The subjects were pharmacists who have their own pharmacies in Seoul and Gyeonggi Province. Of 400 questionnaires distributed to the respondents, 392 were used in the final analysis except 8 with insufficient answers. The empirical analysis results showed that perceived security, perceived cost, and social influence had significant impacts on trust, while social influence and perceived cost had significant impacts on perceived risk. Among RFID use factors, social influence, perceived security, and perceived risk had significant impacts on intention to use in mobile RFID environment. Trust and perceived cost did not have a significant impact. The summary of analysis results, implications, and limitations are presented in the conclusion.

Basic : Lactococcus garvieae septicemia with Liver abscess in an immunocompetent patient (초)

( Sang Wook Lee ),( Hyung Joon Kim ),( Suh Yoon Yang ),( Hyun Woong Lee ),( Bong Ki Cha ),( Beom Jin Kim ),( Hyoung Chul Oh ),( Chang Hwan Choi ),( Jeong Wook Kim ),( Jae Hyuk Do ),( Jae Gyu Kim ),( S 대한간학회 2011 Clinical and Molecular Hepatology(대한간학회지) Vol.17 No.3(S)

HCV, Alcoholic : PE-080 ; Hepatitis C virus infection sensitizes human hepatocytes to TRAIL-induced apoptosis in a caspase 8 and 9 dependent manner

( Jae Young Jang ),( Eun Kyung Cho ),( Soung Won Jeong ),( Ji Eun Lee ),( Jin Woo Choo ),( Jin Nyoung Kim ),( Soon Ha Kwon ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang-woo Cha ),( Young Seok Kim ),( Y 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background/Aim: Chronic hepatitis C virus (HCV) infection increases hepatocyte apoptosis in response to the Tumor Necrosis Factor-alpha (TNF-α) family. We investigated the mechanism of apoptosis, particularly the intracellular pathway in JFH-1 (HCV) infected Huh 7.5.1 cells using recombinant TRAIL (TNF-Related Apoptosis-Inducing Ligand) and caspase inhibitors. Methods: Apoptosis was measured by western blotting for cleaved PARP. Expression of caspase 8, 9 and inactivation of caspase pathways using recombinant TRAIL and caspase inhibitors were assessed, respectively. Results: 1) We found increased cleaved PARP in JFH-1 infected Huh 7.5.1 cells compared to Huh 7.5.1 cells (p < 0.001). 2) Optimal concentration and time of recombinant TRAIL were 100 ng/mL of concentration and 4 hours of incubation time. 3) Cleaved PARP in JFH-1 infected Huh 7.5.1 cells in the presence of recombinant TRAIL was more increased compared to those without recombinant TRAIL (p < 0.001). 4) Both TRAIL R1 (DR4) and R2 (DR5) mRNA and protein expression were increased in JFH-1 infected Huh 7.5.1 cells compared to Huh 7.5.1 cells (p < 0.05). 5) HCV induced apoptosis was decreased by DR4 and DR5 siRNA (p < 0.05). 6) Increased caspase 8 and 9 activity was found in JFH-1 infected Huh 7.5.1 cells in the presence of recombinant TRAIL compared to those without recombinant TRAIL (p < 0.001). 7) The degree of increased caspase 8 and caspase 9 activity was similar in JFH-1 infected Huh 7.5.1. 8) Caspase 8 and 9 inhibitors blocked apoptosis induced by HCV comparably to pancaspase inhibitor. Conclusion: Our findings indicate that hepatocyte apoptosis is increased in the presence of HCV compared to Huh 7.5.1 cells alone, and this increase is mediated by sensitizing human hepatocytes to TRAIL-induced apoptosis in a caspase 8 and 9 dependent manner.

Impact of non-hepatic hyperammonemia on mortality in intensive care unit patients: a retrospective cohort study

( Jae Heon Kim ),( Hankyu Jeon ),( Sang Soo Lee ),( I Re Heo ),( Jung Woo Choi ),( Hee Jin Kim ),( Ra Ri Cha ),( Jae Min Lee ),( Hyun Jin Kim ) 대한내과학회 2021 The Korean Journal of Internal Medicine Vol.36 No.6

Background/Aims: The effect of hyperammonemia on the mortality in patients with liver cirrhosis is well documented. However, little is known about the impact of hyperammonemia on mortality among intensive care unit patients without hepatic disease. We aimed to investigate factors associated with non-hepatic hyperammonemia among intensive care unit patients and to evaluate the factors related to the 7- and 90-day mortality. Methods: Between February 2016 and February 2020, 948 patients without hepatic disease who had 972 episodes of admission to the intensive care unit were retrospectively enrolled and classified as hyperammonemia grades 0 (≤ 80 μg/dL; 585 [60.2%]), 1 (≤ 160 μg/dL; 291 [29.9%]), 2 (≤ 240 μg/dL; 55 [5.7%]), and 3 (> 240 μg/dL; 41 [4.2%]). Factors associated with hyperammonemia and the 7- and 90-day mortality were evaluated by multivariate logistic regression analysis and Cox regression analysis, respectively. Kaplan-Meier survival curves for the 7- and 90-day mortality were constructed. Results: The independent risk factors for hyperammonemia were male sex (odds ratio, 1.517), age (0.984/year), acute brain failure (2.467), acute kidney injury (1.437), prothrombin time-international normalized ratio (2.272/unit), and albumin (0.694/g/dL). The 90-day mortality rate in the entire cohort was 24.3% and gradually increased with increasing hyperammonemia grade at admission (17.9%, 28.2%, 43.6%, and 61.0% in patients with grades 0, 1, 2, and 3, respectively). Additionally, non-hepatic hyperammonemia was an independent predictor of the 90- day mortality in intensive care unit patients. Conclusions: Non-hepatic hyperammonemia is common (39.8%) and associated with the 90-day mortality among intensive care unit patients.

HCV, Acute, LT : Hepatitis C Virus Infection Elevates TRAIL-DR4/DR5-Induced Apoptosis via Caspase-8 Activation

( Jae Young Jang ),( Seong Jun Kim ),( Eun Kyung Cho ),( Soung Won Jeong ),( Yun Nah Lee ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ),( 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background: Persistent hepatitis C virus (HCV) infection induces apoptosis of human hepatocytes. We and others have recently shown that HCV infection sensitizes host cells to mito- chondrial apoptosis via TRAIL death receptor-R1 (DR4)/-R2 (DR5). However, critical determinants of TRAIL-DR4/DR5- mediated apoptosis induced by HCV infection still remain to be clarified. Here we extend our study to examine if HCV infection elevates TRAIL-DR4/DR5-mediated apoptosis via caspase- 8 activation. Methods: Using HCV JFH-1 cell-culture system (HCVcc), the expression level of DR4 and DR5 in HCV-infected Huh7.5.1 cells was analyzed by qRT-PCR and Western blotting. Caspase activity assay and Western blotting were conducted for analysis of TRAIL-DR4/DR5-mediated caspase activation cascade in infected cells by using recombinant TRAIL, caspase inhibitors, and siRNAs specific to DR4 and DR5. Results: HCV infection stimulates DR4 and DR5 gene expression at both levels of transcription and translation. HCV-induced apoptosis via DR4/DR5 was evidenced by the reduction of caspase-3/7 activity by both DR4 and DR5 silencing and the increase of cleavage of caspases including caspase-8, caspase-9, and caspase-3 by TRAIL treatment. Treatment of infected cells with caspase-8 specific inhibitor resulted in the decline of HCV-induced cleavage of PARP and Bid, a pro-apoptotic protein. Conclusions: Our data identify that HCV infection elevates- TRAIL-DR4/DR5-mediated apoptosis of human hepatoma cells via caspase-8 activation. Given the importance of apoptosis in promoting hepatic fibrogenesis, these results suggest potential utility for TRAIL inhibition in chronic hepatitis C.

Simple method for the temporal characterization of amplified spontaneous emission in femtosecond terawatt Ti:sapphire lasers

Cha, Yong-Ho,Lee, Yong-Woo,Nam, Sung Mo,Han, Jae Min,Rhee, Yong Joo,Yoo, Byung Duk,Lee, Byoung Chul,Jeong, Young Uk The Optical Society 2007 Applied Optics Vol.46 No.28

<P>We have analyzed the temporal characteristics of amplified spontaneous emission (ASE) in femtosecond terawatt Ti:sapphire lasers by using a simple method based on fast photodiodes. Instead of measuring ASE directly with fast photodiodes, we created a narrow gap in the spectrum of seed pulses and, after amplification, detected the pure ASE signal through the gap by using a fast photodiode covered with a bandpass filter with high transmission at the gap. Because the detected ASE signal was completely separated from amplified main pulses, preceding and even trailing ASEs could be characterized quantitatively in a single-shot measurement. We believe that our method is a good alternative or a complement to conventional methods for ASE measurements.</P>

Factors Influencing Student Satisfaction with the Fieldwork Course in LIS in South Korea

Cha, Sung-Jong,Bae, Kyung-Jae Korea Institute of Science and Technology Informat 2020 Journal of Information Science Theory and Practice Vol.8 No.2

This study attempts to draw the implications of student satisfaction with fieldwork courses in the library and information science (LIS) program in South Korea by determining the level of satisfaction experienced by students with practicums at a library and information service agency. LIS students with fieldwork experience were surveyed to assess their satisfaction and the factors affecting their satisfaction. Results from a multiple regression analysis revealed that from among factors of practicum settings, major-related attributes, and general attributes of respondents, "satisfaction with majors" and "interpersonal relationships" had a significant effect on satisfaction with practicums. Examining the six components of satisfaction with the course revealed that "practicum program" and "practicum setting" had a significant relationship with overall satisfaction. A selective intervention program to improve satisfaction with the course needs to be developed based on factors influencing overall satisfaction, focusing on "interpersonal relationship" and "satisfaction with majors.

Association of α-adducin Gly460Trp polymorphism with coronary artery disease in a Korean population

Cha, Seung-Hun,Kim, Hung-Tae,Jang, Yangsoo,Park, Sungha,Kim, Jae-Jung,Song, Min Young,Park, Jin-Hyoung,Ryu, Ha-Jung,Park, Hyun-Young,Yoon, Sung-Joo Kim,Kimm, Kuchan,Lee, Jong-Keuk,Oh, Bermseok Lippincott Williams & Wilkins 2007 Journal of Hypertension Vol.25 No.12

OBJECTIVE: Coronary artery disease is caused by multiple genetic and environmental factors. The disease is also closely associated with cardiovascular conditions such as hypertension. In order to investigate any possible role of hypertension candidate genes in the disease development and progression, we examined the association of the polymorphisms of 31 hypertension candidate genes with coronary artery disease. METHODS: Genetic polymorphisms of 31 hypertension candidate genes were initially screened by resequencing DNA samples from 24 unrelated individuals in a Korean population. Association analysis was performed using 1284 unrelated Korean men, including 749 coronary artery disease subjects and 535 normal healthy controls. RESULTS: We identified a total of 409 single nucleotide polymorphisms including 40 nonsynonymous single nucleotide polymorphisms, 32 insertions/deletions and four microsatellites. Among 40 nonsynonymous single nucleotide polymorphisms, 29 were examined for an association with coronary artery disease. A significant association with coronary artery disease was observed in a polymorphism of the ADD1 gene (Gly460Trp; +29017G/T) (odds ratio 0.71–0.81; P = 0.01–0.04). The same polymorphism was also associated with the number of arteries with significant coronary artery stenosis in the coronary artery disease patients (P = 0.01) as well as the increase in systolic blood pressure (P = 0.02). CONCLUSIONS: The ADD1 Gly460Trp polymorphism is significantly associated with an increased risk of coronary artery disease as well as blood pressure, indicating that ADD1 plays a role in the pathogenesis of coronary artery disease as well as hypertension.

Anti-melanogenesis in B16F0 Melanoma Cells by Extract of Fermented Cordyceps militaris Containing High Cordycepin

Jae-Young Cha(차재영),Sung-Young Kim(김성영) 한국생명과학회 2013 생명과학회지 Vol.23 No.12

본 연구는 Monascus purpureus (Mp), Aspergillus oryzae (Ao), Aspergillus kawachii (Ak) 및 Rhizopus oryzae (Ro) 균주로 Cordycepin-고함유 동충하초(Cordyceps militaris)(CMα)를 발효시켜 수용성 추출물을 얻어 페놀화합물 및 플라보노이드 농도와 항산화 및 티로시나제 저해 활성을 측정한 결과 Ak로 발효시킨 CMα (AkF-CMα)에서 각각 46 mg/g 및 093 mg/g과 6274% 및 7997%로 가장 우수한 효과를 나타내었다. 이러한 결과로부터 AkF-CMα를 선택하여 멜라닌 세포(B16F0 mouse melanoma cell)에서 미백효과를 검토하였다. 양성 대조구 arbutin 처리 B16F10 melanoma 세포는 92% 이상의 세포 생육과 43%의 멜라닌 생성 억제 효능을 보였고, AkF-CMα 1, 3 및 5 mg/ml 처리 시 멜라닌 생성은 각각 35, 45 및 53% 억제되었다. 또한 AkF-CMα은 멜라닌 세포 내 tyrosinase 활성과 mushroom tyrosinase 활성 모두를 저해시켰고, 멜라닌 생성 관련 tyrosinase 단백질 발현량도 무첨가군에 비해 처리 농도 의존적으로 억제되었다. 이상의 결과에 따라 Aspergillus kawachii 균주로 발효시킨 Cordycepin-고함유 동충하초(Cordyceps militaris)의 수용성 추출물은 미백 화장품 소재로 개발 가능성이 높은 것으로 사료된다. To find a novel skin whitening agent, the effect of cordycepin-enriched Cordyceps militaris (CMα) extract fermented by fungi on anti-melanogenesis in B16F0 mouse melanoma cells was investigated. Fermented CMα was prepared with fungi, including Monascus purpureus (Mp), Aspergillus oryzae (Ao), Aspergillus kawachii (Ak), and Rhizopus oryzae (Ro), respectively. When the content of the phenolics and the flavonoids and the activities of the antioxidant and the mushroom tyrosinase inhibition were measured in the CM fermented by Ak (AkF-CM), the highest content of the phenolics was 46 mg/g dry weight and the highest content of the flavonoids was 0.93 mg/g; the highest activity of the DPPH radical scavenging was 62.74% and the highest activity of the mushroom tyrosinase inhibition was 79.97% CMαCMα. From this result, AkF-CMα exhibited the highest mushroom tyrosinase inhibitory activity and so it was used in subsequent anti-melanogenesis. B16F0 melanoma cells were treated with 1-10 mg/ml concentrations of AkF-CMα and 200 μM arbutin as the positive control. The melanin content and cell viability of the melanoma cells by arbutin treatment decreased to 43% and 92% of the control, respectively. AkF-CMα treatment at 1, 3, and 5 mg/ml concentrations decreased the extracellular melanin release induced by IBMX treatment by 35%, 45%, and 53%, respectively. AkF-CMα showed inhibitory activity against both intracellular tyrosinase in melanoma cells and mushroom tyrosinase. AkF-CMα reduced the protein level of tyrosinase in the IBMX-stimulated cells. These results indicate that AkF-CMα suppressed the activity and protein content of cellular tyrosinase and decreased the total melanin content in cultured B16F0 melanoma cells.

Productivity Loss of Rheumatoid Arthritis Patients according to the Their Stages of the Disease Activity Score

( Sang-Cheol Bae ),( Jin-Hye Cha ),( Jung-Yoon Choe ),( Sung Jae Choi ),( Soo-Kyung Cho ),( Won-Tae Chung ),( Chung-Il Joung ),( Young-Ok Jung ),( Young Mo Kang ),( Dong-Wook Kim ),( Jinseok Kim ),( Y 대한류마티스학회 2018 대한류마티스학회지 Vol.25 No.2

Objective. Productivity loss was compared by 3-stage of disease activity and associations between higher disease activity and high productivity loss were identified. Methods. Data were extracted from Rheumatoid Arthritis (RA) Patient-reported Outcomes Research, which enrolled 2,000 RA patients (>20-year) on disease-modifying-antirheumatic-drugs (DMARDs) (≥ 6-month) from December 2012 to June 2013. This included 1,457 RA patients with the disease activity score (DAS-28-ESR) in their medical charts. Productivity loss in time and indirect cost was estimated using The World Health Organization Health and Work Performance Questionnaire (HPQ). Baseline characteristics and productivity loss outcomes were compared according to DAS-28-ESR groups. Results. 84.4% were females, 54.2% had low DAS-28-ESR (<3.2), and 38.2% and 7.6% had moderate (3.2∼5.1) and high DAS-28-ESR (>5.1). Patients with moderate to high DAS-28-ESR had higher lost productivity time (LPT) and monthly costs of LPT than those with low DAS-28-ESR (time in hours: 110.0±58.4 vs. 132.4±57.2 vs. 71.5±52.0, p<0.0001; monthly costs of LPT in 1,000 Korean won: 1,097±607 vs. 1,302±554 vs. 741±531, p<0.0001). Multiple regression analyses revealed significant associations with high LPT in high (adjusted odds ratio [OR]=3.87, 95% confidence interval [CI]: 2.18∼6.87) and moderate DAS-28-ESR (adjusted OR=1.88, 95% CI: 1.41∼2.52) compared to low DAS-28-ESR. In addition, positive associations with high monthly costs of LPT were observed in high (adjusted OR=3.45, 95% CI: 1.98∼5.99) and moderate DAS-28-ESR (adjusted OR=1.93, 95% CI: 1.43∼2.54) compared to low DAS-28-ESR. Conclusion. Timely therapeutic strategies should be taken into consideration given that the RA patients with moderate to high DAS-28-ESR showed strong associations with high productivity loss for effective management of RA. (J Rheum Dis 2018;25:122-130)