항정신병약물을 처음으로 사용하는 초발 정신증 환자에서 나타나는 체중 및 대사성 지표의 변화 : 후향적 연구

김우진,심주철,공보금,강제욱,문정준,김정은,김민걸,박민경,김성진,김현정,정도운 대한생물치료정신의학회 2011 생물치료정신의학 Vol.17 No.2

Objectives:This study was to identify weight & metabolic changes in first-episode psychotic patients with antipsychotics use and investigate the differences of weight & metabolic changes between first-episode psychotic patients and controls with antipsychotics use. Method:In this retrospective study, twenty eight first-episode psychotic patients and twenty eight controls with schizophrenia, schizoaffective or bipolar disorder defined by DSM-IV criteria were included. Information about demographic and clinical characteristics of the subjects was gathered from the medical records. Also body weight, sBP, dBP, fasting glucose, lipid profile and WBC count were evaluated before and after antipsychotics use. Rogistic regression was conducted to assess factors associated with weight gain. Results:First-episode psychotic patients showed more weight and BMI changes than controls after antipsychotics use, and these changes continue over 12 months. On the other hand, there were no significant factors associated with weight gain. Conclusion:The results of present study suggest that antispychotics is one of the major causes inducing weight gain of psychotic patients and antipsychotics-induced weight gain is more vulnerable to drug-naive first-episode psychotic patients.

블랙푸드가 첨가된 약콩청국장분말의 영양소 및 생리활성물질 분석

공현주(Hyun-Joo Kong),박현숙(Heyun-Sook Park),김태훈(Tae-Hoon Kim),신승렬(Seung-Ryeul Shin),홍주연(Ju-Yeon Hong),양경미(Kyung-Mi Yang) 한국식품영양과학회 2013 한국식품영양과학회지 Vol.42 No.11

본 연구는 대두청국장분말, 약콩청국장분말, 그리고 흑미, 흑임자, 다시마와 같은 블랙푸드를 첨가한 약콩청국장분말의 일반성분과 영양소 및 항산화 물질을 분석ㆍ비교하여 향후 알츠하이머성 치매관리나 인지능력 향상을 위한 블랙푸드가 첨가된 청국장분말의 in vivo 실험을 위한 기초자료로 활용하고자 실시하였다. 그 결과 대두와 약콩청국장에 비해서 블랙푸드를 첨가한 약콩청국장에서 수분함량이 높았으며, 유리당 중 sucrose, fructose, glucose, maltose 함량이 높았다. 또한 수용성과 조단백질 함량은 P<0.05 수준에서 약콩청국장과 블랙푸드를 첨가한 약콩청국장에서 높았다. 불포화지방산과 포화지방산의 비율은 대두청국장에 비해서 약콩청국장과 블랙푸드를 첨가한 약콩청국장에서 높았다. 무기질 함량은 대두청국장이 가장 높았으나 약콩청국장에서는 Zn, Cr, Mn이, 블랙푸드를 첨가한 약콩청국장에서는 Fe의 함량이 높았다. Isoflavone 중 diadzein 함량은 약콩청국장에서 가장 높았으나, genistein과 anthocyanin 함량은 블랙푸드를 첨가한 약콩청국장에서 가장 높았다. 따라서 본 연구에서는 일반 및 영양적 특성에서 대두청국장에 비해서 블랙푸드로 만든 청국장분말이 유리당과 뇌조직의 구성과 기능에 필요한 수용성 및 단백질 함량과 불포화지방산과 포화지방산의 비율이 높을 뿐만 아니라 우수한 항산화력으로 보고되고 있는 diadzein, genistein 그리고 anthocyanin 함량이 높은 것으로 나타났다. 그러므로 향후 알츠하이머성 치매의 원인 및 치료기전으로 보고되고 있는 산화적 스트레스조절에 대한 in vivo 실험에 블랙푸드청국장분말을 활용할 수 있을 것으로 판단된다. This study is carried out to investigate the analysis of nutrition and antioxidants of soybean Chungkukjang powder (SCP), Yak-Kong Chungkukjang powder (YCP) and Yak-Kong Chungkukjang powder added black foods (YCBP) for the management of Alzheimer"s disease. The water content of YCBP was higher than that of SCP and YCP. The soluble and crude protein contents of YCP were the highest among three Chungkukjang powders. In SCP, YCP and YCBP, the ratios of unsaturated and saturated fatty acids were 5.17, 5.76, and 5.78, respectively. The mineral content of SCP was higher than that of YCP and YCBP. Antioxidants analysis showed that the content of diadzein was the highest in YCP and genistein, and anthocyanin contents were higher than the others in YCBP. In conclusion, YCP and YCBP had higher contents than SCP in soluble and crude proteins, and unsaturated fatty acids, which are needed for composition and function of the brain tissue. Also, it was found that the contents of diadzein, genistein and anthocyanin with outstanding antioxidative ability were high. Thus, this study suggests that Chungkukjang powder, which is made with Yak-Kong, black sesame, black rice and sea tangle, can be utilized for in vivo experiment for the control of oxidative stress, reported as cause and therapy for Alzheimer"s Disease.

Surface plasmon- resonance analysis for the interaction between human sweet taste receptors and sweeteners

Hyun-Joo Jo,Su-Yeon Kim,Ju-Hee Jung,Kwang-Hoon Kong 한국분석과학회 2017 학술대회논문집 Vol.2017 No.11

ANALYSIS OF ALLELE AND GENOTYPE FREQUENCIES AT TWO POLYMORPHIC LOCI ( HLA - DQα AND D1S80 ) IN KOREAN POPULATION BY PCR AMPLIFICATION

Hyun Suk Nam,Eun Hee Kim,Kong Joo Lee 생화학분자생물학회 1993 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

A Phi Class Glutathione S-transferase from Oryza sativa (OsGSTF5): Molecular Cloning, Expression and Biochemical Characteristics

( Hyun Young Cho ),( Hae Joo Lee ),( Kwang Hoon Kong ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.4

Detection of Fragment Length Polymorphism of the VNTR Loci D1S80 and D2S123 by PCR Amplification , PAGE and Silver Staining

Hyun Suk Nam,Eunhee Kim,Wan Hee Yoon,Kong Joo Lee 생화학분자생물학회 1995 BMB Reports Vol.28 No.4

The highly polymorphic variable number of tandem repeat (VNTR) loci in the human genome are informative markers for the genetic characterization of individuals in the paternity test and forensic science as well as for the study of human disease. In this study, VNTR loci D1S80 and D2S123 have been amplified by PCR and the amplified length polymorphic alleles were detected with a discontinuous vertical PAGE system and silver staining. For explicit DNA typing, PCR optimization, in which amplification efficiencies are similar over a wide range of allele sizes, non-specific amplifications are minimal, and new longer alleles have high amplification efficiency, has been performed by changing the PCR reaction buffer composition and thermal cycling conditions. It turned out that adding an appropriate amount of Tween 20 and NP40 to the PCR reaction buffer and raising the annealing temperature to 68℃ in thermal cycling made it possible for optimal VNTR loci amplification. A modified PAGE system for VNTR separation was established. Under these conditions, new longer alleles in the D1S80 locus were discovered and D2S123 pattern changes in colorectal tumors were observed. These technical tips are valuable for detecting various amplified fragment length polymorphisms.

Antitumor activity of ZD6474 in a metastatic orthotopic brain tumor model.

Kong, Doo-Sik,Kim, Mi-Hyun,Jeon, Ji-Won,Kim, Shi-Yeon,Kim, Maeng Sup,Joo, Kyeung Min,Park, Kwan,Nam, Do-Hyun D. A. Spandidos 2008 MOLECULAR MEDICINE REPORTS Vol.1 No.3

<P>The objective of this study was to examine the antitumor effect of ZD6474, an orally available inhibitor of the vascular endothelial growth factor receptor-2 (VEGFR-2) and the epidermal growth factor receptor (EGFR), on tumor growth in an orthotopic metastatic brain tumor model. In order to determine the antitumor mechanism of ZD6474 treatment, in vitro and in vivo studies were performed. Human breast carcinoma cells (MDA-MB-435) were injected using direct intracranial (IC) inoculation (5x105 cells/100 ?l) and internal carotid artery (ICA) injection (5x104 cells/100 ?l) in Balb/c-nu female mice. Daily oral treatment with ZD6474 (50 mg/kg) was initiated on day 14 after the establishment of micrometastasis. Mice (n=12 per group) were sacrificed on day 28. Western blot analysis revealed that the autophosphorylation of EGFR and Akt was increasingly decreased with ZD6474 treatment in lung and brain endothelial cells and the MDA-MB-435 cell line. MTT assay also showed that the in vitro antitumor activity of ZD6474 was dependent on EGFR tyrosine kinase inhibition at a higher dose. Daily oral treatment with ZD6474 led to marked inhibition of metastatic tumor growth in the ICA injection and the direct IC inoculation models (median size 3.5 mm3, range 1.6-13.9 mm3) as compared to the control group (median size 62.4 mm3, range 11.5-206.9 mm3). These results suggest that simultaneous inhibition of both the EGFR and VEGFR-2 signaling pathways has a valuable therapeutic effect through its inhibition of the growth of metastatic brain tumors.</P>

Endometrial surveillance strategy for detection of tamoxifen-related uterine cancers

( Hyun-ji Bae ),( Joo-hyuk Son ),( Jeeyeon Kim ),( Tae-wook Kong ),( Suk-joon Chang ) 대한산부인과학회 2022 대한산부인과학회 학술대회 Vol.108 No.-

Objective: Tamoxifen-treated breast carcinoma survivors are at elevated risk of uterine cancers. Whether to recommend routine surveillance for uterine abnormalities in tamoxifen-treated breast carcinoma survivors is still under debate. This study aimed to evaluate the clinical characteristics of tamoxifen-related uterine cancers and investigated proper surveillance strategy. Methods: We retrospectively reviewed medical records of patients visited gynecologic cancer center after tamoxifen treatment from 2010 to 2021. Uterine cancer incidence and clinical characteristics including oncologic outcomes, duration of tamoxifen use and follow-up intervals were analyzed according to the initial symptoms. Results: A total of 2,349 patients with tamoxifen treated patients were confirmed during the study periods. The patients median age was 46 (22-87). Endometrial polyp, hyperplasia, endometrial intraepithelial neoplasia, and uterine malignancy were found in 252 (10.7%), 100 (4.2%), 44 (1.8%) and 14 (0.6%) patients respectively. Median age of the uterine cancer patients was 50 (37-61) and most of them were diagnosed in the early stage (stage I, 92.8%). However, only 8 (57%) patients were diagnosed after menopause and accompanied with presenting symptoms. The duration from tamoxifen use to the cancer diagnosis was 49 months (12-81) and 30% of the patients of were diagnosed after completion of tamoxifen. The duration from the completion of tamoxifen to the cancer diagnosis was median 10.5 (1-35) months. Among the patients without presenting symptoms, 50% received 6 months, 33% received 12 months, and 17% received 18 months follow-up exam. Conclusion: The number of asymptomatic or pre-menopause patients was relatively high enough to allow routine gynecologic screening in patients treated with tamoxifen. Considering the prognosis, annual surveillance can be suggested, and it should be continued at least 1-year after cessation of tamoxifen, possibly extended up to 3-years.

GO-27 : Prediction of lymph node metastasis in patients with apparent early endometrial cancer

( Joo Hyuk Son ),( Su Hyun Kim ),( Suk Joon Chang ),( Tae Wook Kong ),( Jiheum Paek ),( Hee Sug Ryu ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: The purpose of this study was to the investigate the incidence of lymph node metastasis in patients who were presumed to have endometrial cancer confined to the uterine corpus without enxtrauterine involvement and to evaluate preoperative clinicopathological factors predicting lymph node metastasis. 방법: We identified 142 consecutive patients with endometrial cancer between January 2000 and February 2013. All patents demonstrated endometrioid adenocarcinoma with grade 1 or 2 on preoperative endometrial biopsy. Preoperative magnetic resonance imaging (MRI)showed that tumors were confined to the uterine corpus with superficial myometrial invasion(less than 50%) and there was no any evidence of extrauterine metastasis. All patients had complete staging procedures ? total hysterectomy, adnexectomy, peritoneal cytology, bilateral pelvic lymphadenectomy, and para-aortic lymphadenectomy- and were surgically staged according to the 2009 FIGO staging system. Clinical and pathologic data were obtained from medical records and statistically analyzed. 결과: Of 142 patients, 127 patients (89.4%) presented with stage 1A, 8 (5.6%) had stage IB, 3 (2.1%) had stage II, and 4 (2.8%) had stage III diseases. Three patients (2.1%) had lymph node metastasis ? 2 IIIC1 and 1 IIIC2 diseases. Preoperative tumor grade was upgraded in 8 patients (5.6%) and 27 patients (19%) presumed to have no or superficial myometrial invasion on preoperative MRI postoperatively showed deep myometrial invasion. Age, preoperative tumor grade, and myometrial invasion less than 50% on preoperative MRI were not associated with lymph node metastasis. High preoperative serum CA-125 level (>35 IU/mL) was a statistically significant factor predicting lymph node metastasis on univariate and and multivariate analyses. Lymph node metastasis was only found in patients with preoperative grade 2 tumors or high serum CA-125 level. 결론: Preoparative tumor grade and serum CA-125 level can predict lymph node metastasis in apparent early endometrial cancer patients who had endometriod histology, grade 1- 2 tumor, and superficial myometrial invasion on preoperative on preoperative MRI. Keywords Tumor grade, CA-125, Lymph node metastasis, Early endometrial cancer

Session F : Eukaryotic Gene Structure and Expression : DEVELOPMENT OF HETERODUPLEX ANALYSIS TO DETECT POLYMORPHISMS AND MUTATIONS IN THE HLA DQ α GENE

Hyun Suk Nam,Kyun Eun Kim,Eun Hee Kim,Kong Joo Lee 생화학분자생물학회(구 한국생화학분자생물학회) 1993 추계학술대회집 Vol.- No.-