흰쥐 생체시료 중 5-플루오로우라실 및 테가푸르의 안정성

장지현,박종국,강진형,정석재,심창구,구효정 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.3

5-Fluorouracil (5-FU) is an antimetabolite anticancer agent active against many types of solid tumors. Tegafur (TF), a prodrug of 5-FU, is frequently used in combination with uracil as dihydropyrimidine dehydrogenase (DPD) inhibitory fluoropyrimidine. We studied the stability of 5-FU and TF in biological fluids of rats and determined their bioavailability (BA) and excretion into bile, and urine. The drug concentrations were analyzed by an HPLC method. At room temperature, there was a 14-30% decrease in the concentration of 5-FU and TF in bile, urine, and plasma specimen at 10 and 100 μg/ml over 240 min. No significant difference was noted among the sample types or between two different concentrations of 10 and 100 μg/ml. The decrease in drug concentration was significantly less in samples kept on ice (6-12%) for both drugs. These data indicate that biological fluid samples containing 5-FU or TF in plasma, urine, or bile should be placed on ice during the sample collection. Following these storage guidelines, samples were collected after administration 50 ㎎/㎏ of each drug via i.v. or oral route. BA was 1.5 folds greater for TF (60%) than that of 5-FU (42%). Approximately 0.52 and 3.3% of the i.v. doses of 5-FU and TF was excreted into bile, respectively. Renal clearance of 5-FU was about 16% of its total body clearance. These results suggest that instability of 5-FU and TF in biological fluids should be considered in pharmacokinetic or pharmacogenomic studies.

미세혈관 협심증 환자의 임상상 및 지표에 관한 연구

박상호,온영근,김현정,이태훈,길효욱,김현건,박수진,송해정,한대희,방덕원,신원용,이내희,김성구,권영주 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.1

Background : Microvascular angina or syndrom X is heterogenous and encompasses different pathogenetic entities. Several studies suggest that microvascular angina have good prognosis and occurs predominantly in postmenopausal women. Lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and fibrinogen are the independent risk factor of acute coronary syndrome and are the chief underlying cause of atherosclerosis. We investigated clinical characteristics and markers in patients with microvascular angina. Methods : We studies 23 patients diagnosed with microvascular angina and 16 control group with negative treadmill exercise test and no significant coronary angiogram. We investigated clinical characteristics in patients group and evaluated symptom during a follow-up period of 4.2± 2.5 month. Exercise time in treadmill exercise test was compared between patients and control group. We assessed the level of markers, such as lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, CRP and ESR in two groups. Results : There is no difference in age between two groups. Patients group had a tendency to increase in female, hypertension, DM, and smoking history, but there was no statistical significance difference. Exercise duration time was significantly decreased in patients with microvascular angina (p<0,05). Serum lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholestrol, Triglyceride, ESR, Fibrinogen and Homocystein value were no significant difference between microvascular angina and control group. Only CRP was significantly high in patients with microvascular angina (p<0.05). Conclusion : There was no statistical significance difference in age, sex, hypertension, DM and smoking history between two groups but patients group had a tendency to increase the prevalence in hypertension, DM and smoking history, female. Also, Symptom improvement and disappearance in follow-up was presented in 11 patients of 19(57.9%). Exercise tolerance was worse in patients group than control group. CRP value was significantly higher in patients group than control group. There was no statistical significance difference in lipoprotein (a), total cholesterol, LDL-cholesterol, HDL-cholesterol, triglyceride, homocystein, ESR and Fibrinogen between two groups.

Sediment Provenance and Transport mechanism in Cnetral Yellow Sea Mud

Hyo Jin Koo(구효진),Bu Yeong Lee(이부영),Hyen Goo Cho(조현구) 한국암석학회 2017 한국암석학회 학술발표회 논문집 Vol.2017 No.5

Growth and Phosphorous Absorption Rate of Red Pepper as Affected by Top Dressing Applications of Phosphorus Fertilizer in Different Cropping System

Hyo-Jung Choi,Byung-Koo Ahn,Do-Young Ko,Hyo-Jin Kim,Hyong-Gwon Chon,Ye-Jin Lee,윤석인 한국토양비료학회 2020 한국토양비료학회지 Vol.53 No.3

An experiment was conducted to study the effect of top dressing application of Phosphorus (P) fertilizer onthe growth and P absorption rate of red pepper in different cropping systems of the upland. P fertilizer wasapplied with no treatment as control (CON), basal dressing application (BDA), 2 times-split of top dressingapplications (2-TDA) and 3 times-split of top dressing applications (3-TDA). Red peppers were grown for110 days and soil characteristics and crop growth were investigated every 20 days. Dry matter yield of redpepper significantly increased at the 2-TDA and 3-TDA but decreased at the CON and BDA due probably tothe decrease or increase in available P. In particular, at 3-TDA there was an increase in growth and P absorptionrate of red pepper. The fruit dry matter weight of red pepper receiving 3-TDA was 961.7 kg 10a-1, which wassignificantly higher than those receiving CON and BDA in Rainproof cultivation. In case of the Rainproofcultivation, we found out both P fertilization use effect and top dressing applications effect because there waslow available P content of soil used in the experiment in comparison other cultivation type. Therefore, consideringthe dual goal of optimum crop growth and maximum P distribution instead of immobilization with soil, thisstudy suggests that a testing of a site-specific proper application method of P including top dressing applications isprerequisite to achieving optimum agricultural productivity while minimizing nutrition quality misuse.

A Study on the Burnout Differences by Job Characteristics

Koo Hyo-Jin(구효진),Kim Byeong-Yong(김병용) 한국관광레저학회 2011 관광레저연구 Vol.23 No.6

Regulation of the Endoplasmic Reticulum Stress by BIP/GRP78 is involved in Meiotic Maturation of Porcine Oocytes In Vitro

Hyo-Jin Park,Jae-Young Park,Jin-Woo Kim,Seul-Gi Yang,Jae-Min Jung,Min-Ji Kim,Joung Jun Park,Deog-Bon Koo 한국발생생물학회 2017 발생과 생식 Vol.21 No.4

In the present study, we investigated the role of binding immunoglobulin protein/glucose-regulated protein, 78-kDa (BIP/GRP78)-regulated endoplasmic reticulum (ER)-stress on meiotic maturation and cumulus cells expansion in porcine cumulus-oocyte complexes (COCs). Previously, it has been demonstrated that unfolded protein response (UPR)- related genes, such as molecules involved in ER-stress defense mechanisms, were expressed in matured oocytes and cumulus cells during in vitro maturation (IVM) of porcine oocytes. However, BIP/GRP78-mediated regulation of ER stress in porcine oocytes has not been reported. Firstly, we observed the effects of knockdown of BIP/GRP78 (an UPR initiation marker) using porcine-specific siRNAs (#909, #693, and #1570) on oocyte maturation. Among all siRNAs, siRNA #693 significantly reduced the protein levels of UPR marker proteins (BIP/GRP78, ATF4, and P90ATF6) in porcine COCs observed by Western blotting and immunofluorescence analysis. We also observed that the reduction of BIP/GRP78 levels by siRNA#693 significantly inhibited the meiotic maturation of oocytes (siRNA #693: 32.5±10.1% vs control: 77.8±5.3%). In addition, we also checked the effect of ER-stress inhibitors, tauroursodeoxycholic acid (TUDCA, 200 μM) and melatonin (0.1 μM), in BIP/ GRP78-knockdown oocytes. TUDCA and melatonin treatment could restore the expression levels of ER-stress marker proteins (BIP/GRP78, p-eIF2α, eIF2α, ATF4, and P90ATF6) in siRNA #693-transfected matured COCs. In conclusion, these results demonstrated that BIP/GRP78-mediated regulation of UPR signaling and ER stress plays an important role in in vitro maturation of porcine oocytes.

Anti-oxidant and anti-inflammatory activities of the various kinds of herbal tea

( Jin Wook Lee ),( Hyun Ji Eo ),( Gwang Hun Park ),( Hun Min Song ),( So Hee Woo ),( Mi Kyoung Kim ),( Jung Hye Eom ),( Man Hyo Lee ),( Jeong Rak Lee ),( Jin Suk Koo ),( Jin Suk Koo ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.2

Objectives : Reactive oxygen species (ROS) are involved in a wide spectrum of diseases including chronic inflammation and cancer. In this study, we investigated the antioxidant activities and anti-inflammatory effects of the extracts from the herbal teas such as Lonicera japonica Thunberg (L. japonica), Chrysanthemum morifolium Ramat (C. morifolium), Mentha arvensis L. (M. arvensis), and P.rhizoma. Methods : Anti-oxidant activity was evaluated using DPPH radical scavenging assay and Fe2+ chelating assay. And DNA cleavage assay was performed to evaluate an anti-oxidative effect. Anti-inflammatory effect was performed using NO generation assay and western blot in LPS-stimulated RAW264.7 cell line. Results : L. japonica scavenged DPPH radical by 9.8% at 12.5 μg/ml, 24.8% at 25 μg/ml, 34.3% at 50 μg/ml, 61.1% at 100 μg/ml and 75.8% at 200 μg/ml, respectively. In addition, C. morifolium and M. arvensis removed DPPH radical by 15.6% and 10.4% at 12.5 μg/ml, 34.8% and 22.8% at 25 μg/ml, 66.9% and 43.3% at 50 μg/ml, 87.4% and 69.1% at 100 μg/ml, and 92.1% and 73.2% at 200 μg/ml, respectively. However, P. rhizoma did not affect on DPPH radical scavenging. The Fe2+ chelating activity was highest in L. japonica, but lowest in P. rhizoma among the herbal teas. In addition, the extracts from L. japonica, C. morifolium and M. arvensis inhibited oxidative DNA damage via its anti-oxidant activity. In anti-inflammatory effect, the extracts from C. morifolium inhibited NO production. In addition, it suppressed the NF-κB signaling pathway in LPS-stimulated RAW 264.7 cells. Conclusions : Together, this study indicates that L. japonica, M. arvensis and C. morifolium possess the protective effect against the oxidative DNA damage. Furthermore, C. morifolium exerts an anti-inflammatory effect.

Bisphenol A Induced Mitochondrial Derived Reactive Oxygen Species Plays a Critical Role in Porcine Oocyte Maturation

Hyo-Jin Park,Soo-Yong Park,Jin-Woo Kim,Jae-Young Park,Seul-Gi Yang,Jae-Min Jung,Min-Ji Kim,Deog-Bon Koo 한국동물생명공학회(구 한국동물번식학회) 2016 발생공학 국제심포지엄 및 학술대회 Vol.2016 No.10

An Empirical Analysis on the Relationship between the Korean Popular Cultural and Self-Identity of Korean Adoptees

Koo, Hyo-Jin(구효진),Ju, Hyo-Jin(주효진) 한국지방정부학회 2019 지방정부연구 Vol.23 No.1

본 연구는 한국 대중문화와 자아정체감 간의 관계에 대해 분석하는 것을 목적으로 하였으며, 이에 따라 한국 대중문화가 한국계 해외입양인의 자아 정체감에 미치는 영향을 분석하였다. 본 연구의 대상자는 한국계 해외입양인 51명이었으며, 충분한 연구대상자 수를 확보하기 위해 눈두덩이 표집법이 사용되었다. 본 연구를 위해 Kim(2015)과 Choi, Lee, Han(1986)이 개발한 설문지를 수정 및 보완하여 사용하였으며, 수집된 데이터는 SPSS 22.0을 사용해 분석하였다. 한국 대중문화 경험에 따른 연구 대상자들의 자아 정체감을 분석한 결과, 한국 대중문화는 한국계 해외입양인의 자아정체감에 유의미한 영향을 미치는 것으로 나타났다. 이와 같은 연구 결과를 통해 한국 대중문화가 한국계 해외입양인의 자아정체감 형성에 유용할 수 있음을 알 수 있었으며, 이를 기반으로 해외에 거주하는 한국계 입양인의 한국 문화콘텐츠 대한 접근성이 향상될 수 있도록 연구 확장 및 정책추진의 필요성이 제안되었다. 또한 향후 한국 대중문화 소비 경험이 한국인 교포 내 다른 집단의 자아정체감에 미치는 유의미한 영향을 알기 위한 구체적인 연구의 필요성이 제시되었다. The purpose of this research is to analyze the relationship between the Korean cultural and self-Identity. It has been focusing on the analyzation of the influence of Korean popular culture on the self-identification of overseas transnational Korean adoptees. The participants of this study consisted of 51 overseas transnational Korean adoptees. Snowball sampling was used to locate a sufficient number of participants. A survey was conducted with a questionnaire adapted from the works of Kim(2015) and Choi, Lee, and Han(1986). The results of this study indicate that Korean popular culture has a significant influence on the self-identification of transnational Korean adoptees. Moreover, the findings of this study suggest that Korean popular culture may be useful in the formation of self-identification of overseas transnational Korean adoptees. It is suggested that research about developing relevant policies could be initiated, and research expanded to increase the accessibility of Korean cultural content for transnational Korean adoptees living abroad. Further research is needed to determine whether Korean popular culture consumption has a significant impact on the self-identification of other groups in the Korean diaspora.

Melatonin Reduces the Bisphenol A-induced Superoxide and Improves Oocyte Maturation through Reducing of Mitochondrial Derived Apoptosis in Pigs

Hyo-Jin Park,Jin-Woo Kim,Seul-Gi Yang1,Min-Ji Kim,Ho-Guen Jegal,Joung Jun Park,Deog-Bon Koo 한국동물생명공학회(구 한국동물번식학회) 2018 발생공학 국제심포지엄 및 학술대회 Vol.2018 No.06

Bisphenol-A (BPA) as an organic synthetic compound of exhibiting estrogen-mimicking and hormone-like properties, which is commonly used to induce cellular stress or female reproductive toxicity. In addition, BPA induces the increasing of mitochondrial derived reactive oxygen species (ROS) such as superoxide, and production of these ROS affects to the meiotic maturation and cumulus cells expansion on in vitro maturation (IVM) of porcine cumulus-oocyte complexes (COCs). However, anti-oxidative effect of melatonin for reduction of BPA-induced superoxide on porcine oocyte maturation has not been reported. Therefore, in present study, we confirmed that the reduction of BPA-derived superoxide by melatonin related to the reducing of mitochondria mediated apoptosis on meiotic maturation and cumulus cells expansion of porcine COCs. Then, to investigate the effects of superoxide specific scavenger, Mito-TEMPO, during porcine oocyte maturation progression, COCs cultured in maturation medium with Mito-TEMPO (0.1 μM) after pre-treatment of BPA (75 μM) for 22 h. Reduced meiotic maturation rate and cumulus cells expansion of COCs in the BPA (75 μM) treated group were recovered (p<0.01) by Mito-TEMPO treatment. Also, increasing of mitochondria derived apoptotic factors (AIF, Cleaved Caspase 3 and Cleaved PARP 1) protein levels by BPA treatment were reduced by Mito-TEMPO treatment in porcine COCs maturation. Positive effects of Mito-TEMPO for superoxide reduction on oocyte maturation and reducing mitochondrial apoptosis showed the same pattern in melatonin (0.1 μM) treated COCs. In case of supplemented with BPA and melatonin, superoxide production in COCs was not changed compared to control or melatonin treated groups. Based on these results, we concluded that melatonin as a regulator of superoxide such as Mito-TEMPO improves oocyte maturation through reduction of mitochondria derived apoptosis during IVM of porcine COCs.