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인체 폐암 세포주에 대한 무의 에탄올 추출물의 세포독성
임효빈(Hyo-Bin Yim),이건순(Gunsoon Lee),채희정(Hee Jeong Chae) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.2
무의 에탄올 추출물을 이용하여 폐암에 대한 세포 독성을 조사하였다. 청운 무를 무줄기와 무뿌리로 나누어 수세, 정선, 탈수한 후 에탄올과 물(5 : 5, v/v)의 혼합용매로 추출하고 폐암 세포주 A-549를 사용하여 MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide)법을 사용하여 항암 활성을 분석하였다. 각 well의 570 nm에서의 흡광도를 측정하고 대조군의 흡광도에 대한 백분율을 산출하였다. 실험 결과 무줄기 추출물과 무뿌리 추출물은 모두 A-549에 대한 세포 독성을 가지고 있었다. 무줄기 추출물의 IC_(50)(50% inhibitory concentration)은 0.015%이었고 무뿌리 추출물의 IC_(50)은 0.03%이었다. 동일 농도(0.01%)에서 무줄기 추출물이 무뿌리 추출물보다 A-549에 대한 항암활성이 더 뛰어났다. This study was designed to investigate the cytotoxic effect of ethanonl extract of radish, Raphanuse sativus on human lung cancer cell lines (A-549) using MTT(3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Radish trunk and radish root were extracted by a mixed solvent of water and ethanol (5 : 5, v/v) after drying. The extracts were used for anticancer activity assay. IC_(50) (50% inhibitory concentration) of radish trunk and radish root were 0.015% and 0.03%, respectively. Radish trunk extract showed higher anticancer activity than radish root extract at the same concentration of 0.01%.
김효빈(Hyo Bin Kim),임재욱(Jae Wook Yim),박경순(Kyeong Sun Park) 대한설비공학회 2019 대한설비공학회 학술발표대회논문집 Vol.2019 No.-
In general, power, communication, signal, and control ships are installed in underground facility for public utilities rather than on the ground to improve the beauty and maintenance of the city by co-accepting the electricity, gas and water supply facilities, communication facilities, and sewerage facilities, which are essential to the people"s daily lives. If the underground facility for public utilities is damaged, it will have a huge impact on national security as well as social security. We should analyze the weaknesses and problems of fire prevention and firefighting activities and draw up measures that are suitable for the actual conditions of our country. Since underground facility for public utilities are enclosed and closed, there are problems in which it is difficult to enter due to the occurrence of toxic gases such as zinc, carbon monoxide, and others due to incomplete combustion due to lack of oxygen. Despite repeated accidents, however, the government did not actively revise laws to solve problems or make appropriate plans for disaster prevention measures. Therefore, it is hoped that the fire prevention law and disaster prevention measures suitable for the conditions of the underground facility for public utilities in Korea can be reviewed to improve the performance of existing communities with poor disaster prevention performance and to recognize the importance of the disaster prevention in order to be readjusted in a more appropriate plan for fire response in the future.
Lee, Boeun,Cho, Jae-Hyun,Seo, Hyo Ree,Na, Su Bin,Kim, Jong Hak,Cho, Byung Won,Yim, Taeeun,Oh, Si Hyoung Royal Society of Chemistry 2018 Journal of Materials Chemistry A Vol.6 No.7
<P>Ionic liquids are effective additives in terms of improving the key electrolyte properties including the ionic conductivity and the oxidative solubility of Grignard reagent-based electrolytes for rechargeable magnesium batteries. However, a precise understanding of their working mechanisms remains elusive to date. Here, we unravel for the first time the mechanism behind the drastic improvement of key electrolyte properties of Grignard reagents upon the addition of allyl-functionalized pyrrolidinium-based ionic liquids. We show that the Grignard reagents selectively abstract acidic protons in the allyl functional group to create a series of Mg-complexes that are remarkably stabilized by the formation of resonance structures. Moreover, the properties of the resulting electrolytes are tuned by adjusting the molar concentration of the ionic liquids which determines the chemical reaction pathway for the formation of new Mg-complexes. Overall, this study demonstrates a novel strategic approach for developing highly efficient new electrolyte systems for rechargeable magnesium batteries.</P>
두부 손상 정도에 따른 세포 매개성 면역의 변화에 관한 연구
이시우,손은익,이장철,김동원,임만빈,김인홍,전효진 대한신경외과학회 1995 Journal of Korean neurosurgical society Vol.24 No.10
최근 6개월간의 계명대학교 신경외과에 입원한 심한 전신 손상을 동반하지 않은 두부 손상 환자와 비교군에서 CMI multitest kit를 이용한 DTH 피부검사와 환자의 말초 혈액에서 T세포 및 그 아형과 B세포의 백분율을 조사함으로써 외상정도와 감염과의 상관 관계를 연구한 바 세포 매개성 면역 저하의 지표로 effecter T 임파구인 helper T세포(T4)와 cytotoxic T세포(T8)의 감소 DTH 피부검사에서 반응정도의 감소가 이용될 수 있음을 관찰 하였으며 이러한 현상은 종래의 심한 두부 손상 후 세포 매개성 면역 기능의 저하에 대한 보고와는 달리 비교적 경미한 두부 손상에 의해서도 유발될 수 있음을 알았다. 또한 DTH 피부검사의 Anergy 반응은 감염 가능성을 예측하는데 도움을 주는 지표로 이용 될 수 있음도 보여 주었으며, 통계적 유의성은 떨어지나 effector T 임파구의 백분율 검사는 외상 정도와 비례해 감소하는 경향을 보여 주었다. 그러나 두부 손상 정도가 심할수록 감염율이 증가하는 소견과 연관하여 설명하기에는 DTH 피부검사의 외상정도에 따른 차이성이나, effector T 세포 백분율 측정의 감염군과 비감염군간의 비교에서 각각 특이성이 떨어짐을 보이므로 앞으로 이에 대한 보완이 요구되며, 아울러 세포 매개성 면역이외의 감염에 중요하게 관여하는 다른 요인도 고려한다면, 두 검사 모두 두부 외상 환자에서 세포 매개성 면역성 조사나 환자의 감염에 대한 예측과 치료에 필요한 유용한 정보를 얻는데 비교적 감수성 높은 검사로 사료 되었다. Severe head injury results in the suppression of cellular immunity associated with dysfunctioning of effector lymphocytes, such as helper T cells(CD4) (and cytotoxic T cells(CD8). Despite progress in the management of increased intracranial pressure following head injury, infection remains the most common complication and the primary cause of prolonged hospitalization and death. This study attempts to assess the cellular immune function following head injury according to the degree of severity, and to establish the clinically available parameters of cell mediated immune(CMI) function, which can then be used for coherent prediction of infection risk. Eighteen head injury patients without severe systemic injury, who divided into three subgroups depending on the severity of head injury, were estimated with the use of CMI multitest kit(Merieux Institute, France) to test delayed-type hypersensitivity(DTH) and enumerated the circulating lymphocyte subpopulation(pan T-cell marker CD3, helper T cell marker CD4, cytotoxic T cell marker CD8 and B-cell marker CDl9) on the 1st, 7th, and 21th day of injury. Patients were monitored for evidence of infection for this period. Fourteen patients had no reaction to any antigens of the DTH skin test(anergy) and the remaining four patients had also some degree of anergy. Seven patients became infected and all of them were anergic. There were significant decrease of circulating effector T Iymphocytes, both CD4-positive and CD8-positive cells, within 24 hours of injury in the mild as well as the moderate and severe head injury group. CD4-positive cells were nearly completely recovered by the 7th day of injury. CD8-positive cells had sustained significant decrease even after 3 weeks of injury. There was no significant change in pan T-cells(CD3-positive cells) and B-cells(CDl9-positive cells). The results suggest that DTH skin test and effector T cell enumeration are both relatively simple and highly sensitive parameters for monitoring CMI function. Especially, anergy of DTH skin test can be used for indicator to predict risk of infection. Mild as well as moderate and severe head injuries may result in the suppresion of cellular immunity associated with the dysfunctioning of effector T cell.
납에 노출된 흰쥐에서 Chlorella 섭취가 혈청내 지방성분 및 효소활성에 미치는 영향
김성조,백승화,이주돈,김운성,문광현,임효빈,허종욱,정성윤 한국식품영양학회 2001 韓國食品營養學會誌 Vol.14 No.2
This study was performed to investigate the influence of Chlorella which effected the change of Pb contents, enzyme activity and lipid compounds on the rats fed the beverage involved Pb and the different contented Chlorella added-diets for 18weeks. The rat's weight of 200ppm Pb group was decreased 6.04% and the cause of that was Pb intake. But the rat's weight of Chlorella added-diets+200ppm Pb group was increased 4.02%(p<0.01). When feeding the different(0%, 2%, 5%, 10%) Chlorella added-diets with the Pb contented beverage to the rats, we could know that the Pb contents accumulated on tissue were decreased to 20.70(0%), 12.88(2%), 14.83 (5%) and 19.56(10%), compared with the quantity of Pb taken in. Total-cholesterol, triglyceride, glucose content and AST, ALT, ALP, LDH enzyme activity in serum were the highest on 200ppm Pb group and those were decreased by the order of different (2%∼5%>10%) Chlorella contents +200ppmPb group. A significance was recognized on the level of 1%. Therefore, when rats were exposed to Pb, it was thought that the amount of Chlorella intake was adequate on content 2∼5% for reducing the lead toxicity.