Selection and evaluation of reference genes for gene expression using quantitative real‐time PCR in Mythimna separata walker (Lepidoptera: Noctuidae)

Bai‐Zhong Zhang,Jun-Jie LIU,Xi-Ling CHEN,Guo-Hui YUAN 한국곤충학회 2018 Entomological Research Vol.48 No.5

In order to precisely assess gene expression levels, the suitable internal reference genes must be served to quantify real‐time reverse transcription polymerase chain reaction (RT‐qPCR) data. For armyworm, Mythimna separata, which reference genes are suitable for assessing the level of transcriptional expression of target genes have yet to be explored. In this study, eight common reference genes, including β‐actin (β‐ACT), 18 s ribosomal (18S), 28S ribosomal (28S), glyceraldehyde‐3‐phosphate (GAPDH), elongation fator‐alpha (EF1α), TATA box binding protein (TBP), ribosomal protein L7 (RPL7), and alpha‐tubulin (α‐TUB) that in different developmental stages, tissues and insecticide treatments of M. separata were evaluated. To further explore whether these genes were suitable to serve as endogenous controls, three software‐based approaches (geNorm, BestKeeper, and NormFinder), the delta Ct method, and one web‐based comprehensive tool (RefFinder) were employed to analyze and rank the tested genes. The optimal number of reference genes was determined using the geNorm program, and the suitability of particular reference genes was empirically validated according to normalized HSP70, and MsepCYP321A10 gene expression data. We found that the most suitable reference genes for the different experimental conditions. For developmental stages, 28S/RPL7 were the optimal reference genes, both RPL7/EF1α were suitable for experiments of different tissues, whereas for insecticide treatments, 28S/α‐TUB were suitable for normalizations of expression data. In addition, 28S/α‐TUB were the suitable reference genes because they have the most stable expression among different developmental stages, tissues and insecticide treatments. Our work is the first report on reference gene selection in M. separata, and might serve as a precedent for future gene expression studies.

Association of Polymorphisms in Stress-Related TNFα and NPY Genes with the Metabolic Syndrome in Han and Hui Ethnic Groups

Bu, De-Yun,Ji, Wen-Wu,Bai, Dan,Zhou, Jian,Li, Hai-Xia,Yang, Hui-Fang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.14

Background: Metabolic syndrome (MS) is a cluster of complicated disorders caused by the interactive influencing factors of heredity and environment, which predisposes to many cnacers. Results from epidemic research indicate that stress is tightly related to the pathogenesis of MS and neoplasia. This paper aims to investigate the association between psychological stress and MS with respect to the tumor necrosis factor alpha (TNF${\alpha}$) and neuropeptide Y (NPY) genes in the Han and Hui ethnic groups. Methods: All subjects for this case-control study matched strict enrollment criteria (nationality, gender and age) and lived in the city of Wu Zhong of Ningxia Province in China. The enrolled group contained 102 matched pairs of Hui ethnic individuals and 98 matched pairs of Han ethnic individuals. Enrolled subjects completed the general Symptom Checklist-90 (SCL-90). The TNF${\alpha}$-308G/A variant and NPYrs16147 polymorphism were detected in case (81 males, 119 females) and control (81 males, 119 females) groups by polymerase chain reaction (PCR) amplification. Results: Nine factors of the SCL-90 were found to be statistically different (p<0.05) between case and control groups. The homozygous mutant genotype (AA) and the mutant allele (A) of the TNF${\alpha}$-308G/A gene were less frequently observed in the control population compared to the case group. The odds ratio (95% confidence interval) in "Allele" for MS was 2.28 (1.47-3.53), p=0.0001, while "OR" was 1.11 (0.83-1.47), p=0.15, for the NPYrs16147 gene polymorphism. Conclusions: Psychological stress has been positively associated with MS. A previous study from our group suggested there were differences in the level of psychological stress between Hui and Han ethnic groups. Furthermore, we found that the stress-related TNF${\alpha}$ gene was associated with MS for both Han and Hui ethnic groups. In contrast, NPY may be a possible contributor to MS and associated cancer for the Han ethnic group.

The Effect of Chinese Martial Arts SANDA on the Physical Self-Reliance and Life Satisfaction of University Students

Bai, Hui-Zhong,Song. Jeho 한국스포츠학회 2020 한국스포츠학회지 Vol.18 No.1

본 연구의 목적은 중국 무술 싼다가 지역 대학생들의 신체자존 및 삶의 만족도에 미치는 영향을 규명하기 위해 수행되었다. 연구대상은 산동체육대학(山東體育學院) 2학년 3개 반 비체육전공 여대생반 26명, 체육전공 여대생반 22 명, 체육전공 남대생반 24명, 총 72명의 대학생을 대상으로 비교 실험을 진행하였다. 실험 전, 중, 후 세 번 조사하고, 그 중 실험 대상자는 216명의 학생으로 구성되어 있고, 전체 연구는 중등 단련 강도로 16주간의 무술 싼다 연습을 진행하 였다. 이상과 같은 연구방법을 통해 도출된 연구결과는 다음과 같다. 무술 싼다 단련이 대학생들의 심리건강증진에 도움이 된다는 것을 밝혔다. 조사데이터를 SPSS 통계 분석을 통해 본 결과, 실험 시간에 따라 대학생들의 신체자존감과 생활만족 도에 미친 영향이 다르다는 결과를 보였다. 16주 훈련을 한 여대생의 단련 효과가 8주 단련효과 보다 더 낮았으며, 남학생 의 경우신체자존감 면에서 8주의 단련 효과가 16주보다 뛰어났다. 본 연구를 통하여 무술 싼다 운동이 심리건강증진에 대한 기초자료로 활용될 것으로 사료된다. The purpose of this study was carried out to establish the effect of Chinese martial arts SANDA on the physical self-reliance and life satisfaction of university students. The subjects of the study were 26 non-athletic female college students in three classes of the second year of the Shandong Sports University, 22 female college students majoring in physical education, 24 male college students majoring in physical education, and 72 university students in total. Three surveys before, after, and after the experiment consisted of 216 students, and the whole study conducted 16 weeks of martial arts SANDA practice with secondary training intensity. The results of the studies produced by the above methods are as follows. It revealed that training in martial arts, "SANDA," helps college students improve their psychological health. According to the analysis of SPSS statistics, the impact of the survey data on self-esteem and satisfaction of college students varies depending on the time of the experiment. The 16-week training for female college students was lower than the eight-week training, and the eight-week training for male students was better than the 16th week in terms of physical self-esteem. Through this research, it is believed that the martial art "SANDA Movement" will be used as basic data for the promotion of psychological health.

Correlations Between Fasciology and Yin Yang Doctrine

Hui Tao,Mei-chun Yu,Hui-ying Yang,Rong-mei Qu,Chun Yang,Xin Zhou,Yu Bai,Jing-peng Wu,Jun Wang,Ou Sha,Lin Yuan 사단법인약침학회 2011 Journal of Acupuncture & Meridian Studies Vol.4 No.2

The aim of this study is to explore the correlations between fasciology and yin yang doctrine. Professor Yuan developed fasciology by three-dimensional reconstruction of connective tissue (fascia) in the trunk and limbs of the human body and tracing back to tissue origins in light of biological evolution and developmental biology. Fasciology states that the human body can be divided into two systems: the supporting-storing system and the functional system. This article elaborates on the roles of the two systems and their mutual relationship. The two systems are used to analyze the yin,the yang, and their relationship. The two systems are promoted but also restricted in different contexts. The supporting-storing system is formed by undifferentiated connective tissue and provides undifferentiated cells and nutrients for differentiated cells of the functional system. Thus, the supporting-storing system could be classified as quiet, similar to yin. The functional system continuously maintains the various functional activities of the human body. Thus, the functional system could be classified as active, similar to yang. In interpreting the yin yang doctrine from the point of view of fasciology, yin can be compared with the supporting-storing system and yang can be compared with the functional system.

Bacterial Microbiome Differences between the Roots of Diseased and Healthy Chinese Hickory (Carya cathayensis) Trees

Bai Xiao-Hui,Yao Qi,Li Genshan,Guan Guan-Xiu,Fan Yan,Cao Xiufeng,Ma Hong-Guang,Zhang Mei-Man,Fang Lishan,Hong Aijuan,Zhai Dacai 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.10

Carya cathayensis is an important economic nut tree that is endemic to eastern China. As such, outbreaks of root rot disease in C. cathayensis result in reduced yields and serious economic losses. Moreover, while soil bacterial communities play a crucial role in plant health and are associated with plant disease outbreaks, their diversity and composition in C. cathayensis are not clearly understood. In this study, Proteobacteria, Acidobacteria, and Actinobacteria were found to be the most dominant bacterial communities (accounting for approximately 80.32% of the total) in the root tissue, rhizosphere soil, and bulk soil of healthy C. cathayensis specimens. Further analysis revealed the abundance of genera belonging to Proteobacteria, namely, Acidibacter, Bradyrhizobium, Paraburkholderia, Sphaerotilus, and Steroidobacter, was higher in the root tissues of healthy C. cathayensis specimens than in those of diseased and dead trees. In addition, the abundance of four genera belonging to Actinobacteria, namely, Actinoallomurus, Actinomadura, Actinocrinis, and Gaiella, was significantly higher in the root tissues of healthy C. cathayensis specimens than in those of diseased and dead trees. Altogether, these results suggest that disruption in the balance of these bacterial communities may be associated with the development of root rot in C. cathayensis, and further, our study provides theoretical guidance for the isolation and control of pathogens and diseases related to this important tree species.

Biological characterization of mesenchymal stem cells from bovine umbilical cord

Hui Xiong,Wei Jun Guan,Yue Hui Ma,Chunyu Bai,Shuang Wu,Yuhua Gao,Taofeng Lu,Qingyun Hu 한국통합생물학회 2014 Animal cells and systems Vol.18 No.1

Mesenchymal stem cells (MSCs) are multi-potential cells that are able to proliferate and differentiate into othercell types. Much research has been done on the MSCs from the umbilical cord (UCMSCs) in human, mice, andavian, but little literature has been published about these cells in big livestock. Here, we choose Luxi cattle asthe experimental animal, we describe an external culture of the UCMSCs from it and summarize the biologicalcharacteristics of these cells, e.g., morphologic appearance, surface antigens, colony-forming ability, geneexpression, and differentiation potential were detected via using immunofluorescence and reverse transcriptionpolymerase chain reaction (RT-PCR). The induced cells, osteoblast, lipoblast, hepatocyte, islet cells, andneurocyte were identified by Alizarin red staining, Oil-red-O staining, Periodic acid-schiff staining, andDithizone staining and RT-PCR detection for specific genes. Results suggest that biological characteristics ofthe UCMSCs were similar to those of MSCs previously analyzed. The primary UCMSCs were sub-cultured topassage 32, the UCMSCs expressed gene CD29, CD44, CD73, CD90, and CD166, induced cells illustratedtypical staining, and expressed specific genes, which indicate that the UCMSCs could be a novel alternativesource of MSCs for experimental and clinical applications.

Hippophae rhamnoides L. Leaves Extract Enhances Cell Proliferation and Neuroblast Differentiation through Upregulation of Intrinsic Factors in the Dentate Gyrus of the Aged Gerbil

Bai Hui Chen,Ji Hyeon Ahn,Joon Ha Park,In Hye Kim,Jeong-Hwi Cho,Jae-Chul Lee,Bich-Na Shin,Hyun-Jin Tae,Jung Hoon Choi,Yun Lyul Lee,Moo-Ho Won 한국실험동물학회 2014 한국실험동물학회 학술발표대회 논문집 Vol.2014 No.8

Ethanol extract of <i>Oenanthe javanica</i> increases cell proliferation and neuroblast differentiation in the adolescent rat dentate gyrus

Chen, Bai Hui,Park, Joon Ha,Cho, Jeong Hwi,Kim, In Hye,Shin, Bich Na,Ahn, Ji Hyeon,Hwang, Seok Joon,Yan, Bing Chun,Tae, Hyun Jin,Lee, Jae Chul,Bae, Eun Joo,Lee, Yun Lyul,Kim, Jong Dai,Won, Moo-Ho,Kang Medknow PublicationsMedia Pvt Ltd 2015 Neural regeneration research Vol.10 No.2

<P><I>Oenanthe javanica</I> is an aquatic perennial herb that belongs to the <I>Oenanthe genus</I> in Apiaceae family, and it displays well-known medicinal properties such as protective effects against glutamate-induced neurotoxicity. However, few studies regarding effects of <I>Oenanthe javanica</I> on neurogenesis in the brain have been reported. In this study, we examined the effects of a normal diet and a diet containing ethanol extract of <I>Oenanthe javanica</I> on cell proliferation and neuroblast differentiation in the subgranular zone of the hippocampal dentate gyrus of adolescent rats using Ki-67 (an endogenous marker for cell proliferation) and doublecortin (a marker for neuroblast). Our results showed that <I>Oenanthe javanica</I> extract significantly increased the number of Ki-67-immunoreactive cells and doublecortin-immunoreactive neuroblasts in the subgranular zone of the dentate gyrus in the adolescent rats. In addition, the immunoreactivity of brain-derived neurotrophic factor was significantly increased in the dentate gyrus of the <I>Oenanthe javanica</I> extract-treated group compared with the control group. However, we did not find that vascular endothelial growth factor expression was increased in the <I>Oenanthe javanica</I> extract-treated group compared with the control group. These results indicate that <I>Oenanthe javanica</I> extract improves cell proliferation and neuroblast differentiation by increasing brain-derived neurotrophic factor immunoreactivity in the rat dentate gyrus.</P>

Melatonin improves vascular cognitive impairment induced by ischemic stroke by remyelination via activation of ERK1/2 signaling and restoration of glutamatergic synapses in the gerbil hippocampus

Chen, Bai Hui,Park, Joon Ha,Lee, Yun Lyul,Kang, Il Jun,Kim, Dae Won,Hwang, In Koo,Lee, Choong-Hyun,Yan, Bing Chun,Kim, Young-Myeong,Lee, Tae-Kyeong,Lee, Jae Chul,Won, Moo-Ho,Ahn, Ji Hyeon Elsevier 2018 BIOMEDICINE AND PHARMACOTHERAPY Vol.108 No.-

<P><B>Abstract</B></P> <P>Vascular dementia affects cognition by damaging axons and myelin. Melatonin is pharmacologically associated with various neurological disorders. In this study, effects of melatonin on cognitive impairment and related mechanisms were investigated in an animal model of ischemic vascular dementia (IVD). Melatonin was intraperitoneally administered to adult gerbils after transient global cerebral ischemia (tGCI) for 25 days beginning 5 days after tGCI. Cognitive impairment was examined using a passive avoidance test and the Barnes maze test. To investigate mechanisms of restorative effects by melatonin, neuronal damage/death, myelin basic protein (MBP, a marker for myelin), Rip (a marker for oligodendrocyte), extracellular signal-regulated protein kinase1/2 (ERK1/2) and phospho-ERK1/2 (p-ERK1/2), and vesicular glutamate transporter (VGLUT)-1 (a glutamatergic synaptic marker) in the hippocampal Cornu Ammonis 1 area (CA1) were evaluated using immunohistochemistry. Melatonin treatment significantly improved tGCI-induced cognitive impairment. Death of CA1 pyramidal neurons after tGCI was not affected by melatonin treatment. However, melatonin treatment significantly increased MBP immunoreactivity and numbers of Rip-immunoreactive oligodendrocytes in the ischemic CA1. In addition, melatonin treatment significantly increased ERK1/2 and p-ERK1/2 immunoreactivities in oligodendrocytes in the ischemic CA1. Furthermore, melatonin treatment significantly increased VGLUT-1 immunoreactive structures in the ischemic CA1. These results indicate that long-term melatonin treatment after tGCI improves cognitive deficit <I>via</I> restoration of myelin, increase of oligodendrocytes which is closely related to the activation of ERK1/2 signaling, and increase of glutamatergic synapses in the ischemic brain area.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Melatonin (Mel) improved impaired memory function after ischemia. </LI> <LI> Mel did not protect ischemia-induced (i-i) neuronal loss in the hippocampus. </LI> <LI> Mel recovered i-i damaged myelinated nerve fibers and oligodendrocytes. </LI> <LI> Mel increased ERK1/2 and p-ERK1/2 in oligodendrocytes in i-i hippocampus. </LI> <LI> Mel increased i-i damaged VGLUT-1 in the hippocampus. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Pretreated quercetin protects gerbil hippocampal CA1 pyramidal neurons from transient cerebral ischemic injury by increasing the expression of antioxidant enzymes

Chen, Bai Hui,Park, Joon Ha,Ahn, Ji Hyeon,Cho, Jeong Hwi,Kim, In Hye,Lee, Jae Chul,Won, Moo-Ho,Lee, Choong-Hyun,Hwang, In Koo,Kim, Jong-Dai,Kang, Il Jun,Cho, Jun Hwi,Shin, Bich Na,Kim, Yang Hee,Lee, Y Medknow PublicationsMedia Pvt Ltd 2017 Neural regeneration research Vol.12 No.2

<P>Quercetin (QE; 3,5,7,3′,4′-pentahydroxyflavone), a well-known flavonoid, has been shown to prevent against neurodegenerative disorders and ischemic insults. However, few studies are reported regarding the neuroprotective mechanisms of QE after ischemic insults. Therefore, in this study, we investigated the effects of QE on ischemic injury and the expression of antioxidant enzymes in the hippocampal CA1 region of gerbils subjected to 5 minutes of transient cerebral ischemia. QE was pre-treated once daily for 15 days before ischemia. Pretreatment with QE protected hippocampal CA1 pyramidal neurons from ischemic injury, which was confirmed by neuronal nuclear antigen immunohistochemistry and Fluoro-Jade B histofluorescence staining. In addition, pretreatment with QE significantly increased the expression levels of endogenous antioxidant enzymes Cu/Zn superoxide dismutase, Mn superoxide dismutase, catalase and glutathione peroxidase in the hippocampal CA1 pyramidal neurons of animals with ischemic injury. These findings demonstrate that pretreated QE displayed strong neuroprotective effects against transient cerebral ischemia by increasing the expression of antioxidant enzymes.</P>