성인 남자의 식습관과 혈중 지질 농도에 관한 연구

신승렬,황보진향,박동철 慶山大學校 1997 論文集 Vol.15 No.1

This paper was carried out to study on the food & daily habit score and lipid contents in serum of adult man as basal research for research on the effects of food & daily habits on the lipid contents in serum of adult man. The number of normal, dangerous leval and hypercholesterolemia was each 133(60.5%), 62(28.2%) and 25(11.3%) of adult man. respectively. The mean age. weight. Broca's index(RBW) and Quetelet index(BMI) was 48.4 9.5. 67.7 10.2. 109.1 14.3, 23.6 3.0. respectively. The numbers of good food habits was 5.4 percentage, but those of bad food habits was 35.5 percentage, It was appeared to bad the mode of dietary and hormony of food. The contents of trigryceride and cholesterol in serum was 115.8 and 192.4mg/dℓ, respectively. The numbers of triglyceride contents(90∼120mg/dℓ in serum was 53(24.1%) of the subjects of research, and those of triglyceride contents (150-210mg/dℓ) in serum was 133(60.5%) of the adult man.

Carbon disulfide induces mitochondria-mediated apoptosis in Sertoli-germ cells coculture

Wei Wang,Zhen Zhang,Yinsheng Guo,Yu Dong,Xiaoyu Huang1,Yijun Zhou,Guoyuan Chen 대한독성 유전단백체 학회 2015 Molecular & cellular toxicology Vol.11 No.2

Carbon disulfide (CS2), a common organic solvent, induces a variety of adverse effects in the male reproductive system. In this study, we investigated the cytotoxicity, ultrastructural changes, and potential apoptotic induction mechanisms of CS2 in mixed cultures of Sertoli and germ cells. Sertoli and germ cells were cocultured and treated with CS2 for 24 h. Growth rates were noted, and apoptotic cells were identified by Hoechst 33258 staining. Ultrastructure changes were observed via transmission electron microscopy (TEM). Mitochondrial membrane potential and expressions of apoptosis-related factors (cytochrome c, Bax, Bcl- 2, caspase-3 and caspase-9) were examined by JC-1 staining, western blot, and real-time PCR. The results showed that CS2 treatment was associated with reduced growth rates of Sertoli-germ cells. Ultrastructure changes in Sertoli-germ cells treated with CS2 were typical of apoptosis. In addition, CS2 treatment depolarized mitochondrial membrane potential, upregulated Bax levels and downregulated Bcl-2 levels, released cytochrome c from the mitochondrial intermembrane space to the cytosol, and triggered mitochondria-mediated apoptosis. Subsequently, caspase-9 and caspase-3 were activated, resulting in Sertoli-germ cells apoptosis. The above data suggest that CS2 has adverse effect on the viability of Sertoli-germ cells and induces apoptosis through mitochondrial pathway.

C-type natriuretic peptide attenuates renal osteodystrophy through inhibition of FGF-23/MAPK signaling

Dong Dong Zhang,Yang Fang Wu,Wei Xia Chen,Yao Xu,Si Yan Liu,Huang Huang Luo,Guang Mei Jiang,Yue Wu,Peng Hu 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

Renal osteodystrophy (ROD) occurs as early as chronic kidney disease (CKD) stage 2 and seems ubiquitous in almost all pediatric patients with CKD stage 5. Fibroblast growth factor (FGF)-23, a bone-derived endocrine regulator of phosphate homeostasis, is overexpressed in CKD and disturbs osteoblast differentiation and matrix mineralization. In contrast, C-type natriuretic peptide (CNP) acts as a potent positive regulator of bone growth. In the present study, we infused CNP into uremic rats and observed whether CNP could attenuate ROD through the inhibition of FGF-23 cascades. In uremic rats, CNP administration significantly alleviated renal dysfunction, calcium phosphate metabolic disorders, hypovitaminosis D, secondary hyperparathyroidism, the decrease in bone turnover markers and retarded bone pathological progression. More importantly, within FGF-23/mitogen-activated protein kinase (MAPK) signaling, the fibroblast growth factor receptor-1, Klotho and alternative (STAT-1/phospho-STAT-1) elements were upregulated by CNP, whereas FGF-23, RAF-1/phospho-RAF-1, and downstream (ERK/phospho-ERK and P38/phospho-P38) elements were paradoxically underexpressed in bone tissue. Therefore, CNP exerts a therapeutic effect on ROD through inhibition of FGF-23/MAPK signaling at the RAF-1 level.

ECG PVC Classification Algorithm based on Fusion SVM and Wavelet Transform

Huang Dong,Liao Zhengquan,Li Changbin,Li Dan,Huang Wendong 보안공학연구지원센터(IJSIP) 2015 International Journal of Signal Processing, Image Vol.8 No.1

In the process of ventricular premature beat (PVC) and normal sinus rhythm (NSR) identification base on electrocardiogram (ECG), there exists problems like negative effect from ECG rhythm and low recognition rate. This paper proposes the electrocardiogram PVC classification algorithm based on support vector machine (SVM) and wavelet algorithm. The algorithm uses the wavelet transform to analyze ECG beating model, which is not influenced by the change of ECG waveform. The two feature sets respectively compose of statistical parameters of the wavelet coefficients and the selected wavelet coefficients. PVC and NSR are analyzed by using SVM. The experimental results show that this method improves the recognition rate of ECG.

Transcriptional Profiling of the Trichoderma reesei Recombinant Strain HJ48 by RNA-Seq

Huang, Jun,Wu, Renzhi,Chen, Dong,Wang, Qingyan,Huang, Ribo The Korean Society for Microbiology and Biotechnol 2016 Journal of microbiology and biotechnology Vol.26 No.7

The ethanol production of Trichoderma reesei was improved by genome shuffling in our previous work. Using RNA-Seq, the transcriptomes of T. reesei wild-type CICC40360 and recombinant strain HJ48 were compared under fermentation conditions. Based on this analysis, we defined a set of T. reesei genes involved in ethanol production. Further expression analysis identified a series of glycolysis enzymes, which are upregulated in the recombinant strain HJ48 under fermentation conditions. The differentially expressed genes were further validated by qPCR. The present study will be helpful for future studies on ethanol fermentation as well as the roles of the involved genes. This research reveals several major differences in metabolic pathways between recombinant strain HJ48 and wild-type CICC40360, which relates to the higher ethanol production on the former, and their further research could promote the development of techniques for increasing ethanol production.

A Hybrid Double Self-lift Switched-Coupled Inductor Quasi-Z-Source Inverter

Huang Dong,Xue Pengfei,Chen Guang,Zhang Chaozhong,Zhao Chang,Huang Yuchen,Ding Xinping,Zhang Min 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.4

A hybrid double self-lift switch-coupled-inductor quasi-Z-source inverter (HDSCL-qZSI) is proposed in this paper. The voltage boost capability is higher and the voltage and current stress on components are lower than the existing quasi-Z-source topologies. Owing to the strong current handling ability and step-down coupled-inductor, the magnetic elements are smaller than that of mSSCL-qZSI and SCL-qZSI whose structure are similar to that of HDCSL-qZSI. The operation principles, comparisons of voltage and current stress on components and the size of magnetic elements are analyzed theoretically. Finally, the eff ectiveness of the proposed HDSCL-qZSI was validated by a 1 kW prototype fabricated in the laboratory.

Impacts of Upstream Building Width and Upwind Building Arrangements on Airflow and Pollutant Dispersion in a Street Canyon

Huang, Yuan-dong,Long, Cui,Deng, Ji-tong,Kim, Chang-Nyung Taylor and Francis 2014 Environmental forensics Vol.15 No.1

Impacts of shape and height of upstream roof on airflow and pollutant dispersion inside an urban street canyon.

Huang, Yuan-Dong,He, Wen-Rong,Kim, Chang-Nyung Ecomed 2015 Environmental Science and Pollution Research Vol.22 No.3

<P>A two-dimensional numerical model for simulating flow and pollutant dispersion in an urban street canyon is firstly developed using the FLUENT code and then validated against the wind tunnel results. After this, the flow field and pollutant dispersion inside an urban street canyon with aspect ratio W/H?=?1 are examined numerically considering five different shapes (vaulted, trapezoidal, slanted, upward wedged, and downward wedged roofs) as well as three different roof height to building height ratios (Z H /H?=?1/6, 1/3, and 1/2) for the upstream building roof. The results obtained reveal that the shape and height of an upstream roof have significant influences on flow pattern and pollutant distribution in an urban canyon. A large single clockwise vortex is generated in the canyon for the vaulted upstream roof at Z H /H?=?1/6, 1/3, and 1/2, the trapezoidal and downward wedged roofs at Z H /H?=?1/6 and 1/3, and the slanted and upward wedged roofs at Z H /H?=?1/6, while a main clockwise vortex and a secondary counterclockwise vortex are established for the trapezoidal and downward wedged roofs at Z H /H?=?1/2 and the slanted and upward wedged roofs at Z H /H?=?1/3 and 1/2. In the one-vortex flow regime, the clockwise vortex moves upward and grows in size with increasing upstream roof height for the vaulted, trapezoidal, and downward wedged roofs. In the two-vortex flow regime, the size and rotational velocity of both upper clockwise and lower counterclockwise vortices increase with the upstream roof height for the slanted and upward wedged roofs. At Z H /H?=?1/6, the pollution levels in the canyon are close among all the upstream roof shapes studied. At Z H /H?=?1/3, the pollution levels in the canyon for the upward wedged roof and slanted roof are much higher than those for the vaulted, trapezoidal, and downward wedged roofs. At Z H /H?=?1/2, the lowest pollution level appears in the canyon for the vaulted upstream roof, while the highest pollution level occurs in the canyon for the upward wedged roof.</P>

Phenotypic and genomic characteristics of Brevibacterium zhoupengii sp. nov., a novel halotolerant actinomycete isolated from bat feces

Huang Yuyuan,Dong Lingzhi,Gong Jian,Yang Jing,Lu Shan,Lai Xin-He,Jin Dong,Huang Qianni,Pu Ji,Liu Liyun,Xu Jianguo 한국미생물학회 2022 The journal of microbiology Vol.60 No.10

Two strictly aerobic, Gram-staining-positive, non-spore-forming, regular rod-shaped (approximately 0.7 × 1.9 mm) bacteria (HY170T and HY001) were isolated from bat feces collected from Chongzuo city, Guangxi province (22°20 54 N, 106°49 20 E, July 2011) and Chuxiong Yi Autonomous Prefecture, Yunnan province (25°09 10 N, 102°04 39 E, October 2013) of South China, respectively. Optimal growth is obtained at 25–28°C (range, 4–32°C) on BHI-5% sheep blood plate with pH 7.5 (range, 5.0–10.0) in the presence of 0.5– 1.0% NaCl (w/v) (range, 0–15% NaCl [w/v]). The phylogenetic and phylogenomic trees based respectively on the 16S rRNA gene and 845 core gene sequences revealed that the two strains formed a distinct lineage within the genus Brevibacterium, most closely related to B. aurantiacum NCDO 739T (16S rRNA similarity, both 98.5%; dDDH, 46.7–46.8%; ANI, 91.9–92.1%). Strain HY170T contained MK-8(H2), diphosphatidylglycerol (DPG) and phosphatidylglycerol (PG), galactose and ribose as the predominant menaquinone, major polar lipids, and main sugars in the cell wall teichoic acids, respectively. The meso-diaminopimelic acid (meso-DAP) was the diagnostic diamino acid of the peptidoglycan found in strain HY170T. Anteiso-C15:0 and anteiso-C17:0 were the major fatty acids (> 10%) of strains HY170T and HY001, with anteiso-C17:1A predominant in strain HY170T but absent in strain HY001. Mining the genomes revealed the presence of secondary metabolite biosynthesis gene clusters encoding for non-alpha poly-amino acids (NAPAA), ectoine, siderophore, and terpene. Based on results from the phylogenetic, chemotaxonomic and phenotypic analyses, the two strains could be classified as a novel species of the genus Brevibacterium, for which the name Brevibacterium zhoupengii sp. nov. is proposed (type strain HY170T = CGMCC 1.18600T = JCM 34230T).

Microarray Analysis of the Hypoxia-induced Gene Expression Profile in Malignant C6 Glioma Cells

Huang, Xiao-Dong,Wang, Ze-Fen,Dai, Li-Ming,Li, Zhi-Qiang Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

Hypoxia is commonly featured during glioma growth and plays an important role in the processes underlying tumor progression to increasing malignancy. Here we compared the gene expression profiles of rat C6 malignant glioma cells under normoxic and hypoxic conditions by cDNA microarray analysis. Compared to normoxic culture conditions, 180 genes were up-regulated and 67 genes were down-regulated under hypoxia mimicked by $CoCl_2$ treatment. These differentially expressed genes were involved in mutiple biological functions including development and differentiation, immune and stress response, metabolic process, and cellular physiological response. It was found that hypoxia significantly regulated genes involved in regulation of glycolysis and cell differentiation, as well as intracellular signalling pathways related to Notch and focal adhesion, which are closely associated with tumor malignant growth. These results should facilitate investigation of the role of hypoxia in the glioma development and exploration of therapeutic targets for inhibition of glioma growth.