구강 내 디지털 인상채득을 통한 맞춤형 지대주와 시멘트 합착 후 나사형 임플란트 보철 수복 증례

홍용신,박은진,김선종,김명래,허성주,박지만 대한치과보철학회 2012 대한치과보철학회지 Vol.50 No.1

최근 디지털 인상채득 시스템이 개발되면서 이를 기존의 CAD/CAM 시스템과 접목한 인레이, 온레이, 크라운이나 간단한 계속가공의치 등의 고정성 보철물의 제작이 활발히 소개되고 있다. 그러나 임플란트 보철에서는 주로 기존의 아날로그식 인상채득 후 이를 스캔하여 제작하는 방법이 사용되어 왔으나, 구강 내 디지털 스캔한 데이터를 이용하여 나사형 보철물을 제작하는 술식은 아직 활발히 이루어지지 않았다. 이에 본 증례보고에서는 6명의 환자를 대상으로 구강 내 스캐너인 iTero를 사용하여 디지털 스캔을 시행한 후 그중 다섯명은 맞춤형 지대주와 시멘트 합착 후 나사형 임플란트 보철물(screw-retained implant prosthesis after cementation, or SCRP)을 제작하였고, 임상적으로 기능과 심미적인 측면으로 만족할 만한 결과를 얻었기에 이를 보고하고자 한다. Fixed dental prostheses such as inlay, onlay, crown, and bridge fabricated by CAD/CAM technique combined with digital impressions is getting popular due to the recent rapid progress of digital impression taking system. For the scope of implant prosthesis, however, digital intra-oral scan hasn’t been actively utilized for the fabrication of superstructures. In this case report, 6 cases of titanium-milled custom abutment based on the iTero intra-oral scan data were introduced, five of them were restored with screw-type prosthesis after cementation (SCRP) and the clinical results were satisfactory on restoring the function and esthetics.

뉴럴네트워크를 이용한 이동로봇의 지능제어

김홍래,김용태,한성현 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

This paper presents a new approach to the design of cruise control system of a mobile robot with two drive wheel. The proposed control scheme uses a Gaussian function as a unit function in the fuzzy neural network, and back propagation algorithm to train the fuzzy neural network controller in the framework of the specialized learning architecture. It is proposed a learning controller consisting of two neural network-fuzzy based on independent reasoning and a connection net with fixed weights to simply the neural networks-fuzzy. The performance of the proposed controller is shown by performing the computer simulation for trajectory tracking of the speed and azimuth of a mobile robot driven by two independent wheels.

환경시료로부터 미량 페놀류의 분석을 위한 isoButoxycarbonyl 유도체화 분석방법 적용 및 최적화

김협,홍종기,김용화,김경래 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.1

Eleven phenols including two chlorophenols, eight alkylphenols and bisphenol A were derivatized with isobutylchlomformate to form their isobutoxycarbonyl derivatives. Standard phenol mixture was concentrated for the isebutoxycarbonyl (isoBOC) derivatization and analysed by gas chromatography/mass spectrometry (GC/MS). The recoveries of the derivatization method of alkylphenols, chlorophenols, and bisphenol A were calculated by gas chromatography/mass spectrometry -selected ion monitoring mode using two work-up methods for comparison; shaking and heating method. The linear detector responses were obtained in the concentration range of 5 -400 ng, with correlation coefficients varying from 0.9755-0,9981. Recoveries of the alkylphenols, chlorophenols, and bisphenol A were determined by gas chromatography /mass spectrometry -selected ion monitoring mode using two work-up methods for comparison; the US-EPA method and the isoBOC derivatization method. Eleven phenols in water samples were extracted with dichloromethane and then concentrated. Also, solid-phase extraction (SPE) with XAD-4 and subsequent conversion to isobutoxymbony derivatives for sensitive analysis with the selected ion-monitoring (SIM) mode. The recoveries were 85.1~109.9% and 90.3~126.6% for the US-EPA method and the isoBOC derivatization method, respectively. The method detection limits of bisphenol A for SIM were 0.732 ㎍/ℓ and 0.002 ㎍/ℓ for the two methods above, respectively.

멧돼지 생식후 발생한 선모충증의 가족 내 집단발생사례

김에스더,편래현,박지한,김강호,최일,박홍훈,이용희,용태순,홍성관 대한감염학회 2003 감염과 화학요법 Vol.35 No.3

선모충증은 전 세계적으로 발생하는 기생충 인수공통감염의 한로 선모충의 유충에 감염된 육류를 덜 익혀 섭식시 발생하며 감염 초기에 설사, 복통의 증상이 나타나며 후기에 유충의 체내 이행으로 발열, 근염, 안검 부종 등이 동반된다. 확진은 근육 생검을 하여 피포 유충을 확인하는 것이고 최근에는 특이 항체를 측정하거나 유전자 검사와 같은 다양한 검사가 시도되고 있다. 저자 등은 4명의 한 가족이 야생 멧돼지를 생식한 후 선모충증의 전형적인 임상 증상 및 검사 소견을 보인 가족 내 집단발생사례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Trichinosis is a worldwide parasitic infection caused by ingesting an uncooked raw meat containing viable larvae of nematode Trichinella. Although most cases have been reported in Western countries, the incidence has decreased due to strict control of meat handlings and improved hygiene in these countries. In Korea, the first case of Trichinella spiralis was reported in a patient who ingested an uncooked badger. We have also experienced four cases of Trichinella spiralis infestation among family members who showed typical clinical manifestations and laboratory findings after ingesting raw wild swine meat, and herein report the finding.

RNA Interference of Apolipophorin-III Leads to Knockdown of Antioxidants in Hyphantira cunea

Hong Ja Kim,Young Jin Kang,Yong Min Kwon,Yong Il Kim,In Hee Lee,Byung Rae Jin,Yeon Soo Han,Hyang Mi Cheon,Nam Gyu Ha,Sook Jae Seo 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

Apolipophorin-Ⅲ (apoLp-Ⅲ) is a hemolymph protein whose function is to facilitate lipid transport in an aqueous medium in insect. Recently, apolipophorin-Ⅲ in Galleria mellonella and Hyphantria cunea was shown to play an unexpected role in insect immune activation. We show here a novel possible function/role of apoLp-Ⅲ in insects. To investigate the genes which have a relationship with apoLp-Ⅲ in fall webworm larvae, we reduction of endogenous Hc apoLp-Ⅲ mRNA levels in larvae via RNA interference (RNAi). The RNAi-mediated Hc apoLp-Ⅲ reduction resulted in the reduction of antioxidants, like MnSOD, catalase, and glutathione S transferase as well as immune proteins. In particular, expression of MnSOD commonly decreased in fat body, midgut, and hemocytes following the knockdown of Hc apoLp-Ⅲ, which induced an elevated level of superoxide anion in H. cunea larvae. The observed effect of Hc apoLp-Ⅲ RNAi suggests that Hc apoLp-Ⅲ is related to the action/expression of antioxidants.

Knockdown of apolipophorin-III is caused disruption of mitochondrial membrane potential in Hyphantria cunea

Hong Ja Kim,Yong Il Kim,Yong Min Kwon,In Hee Lee,Byung Rae Jin,Yeon Soo Han,Hyang Mi Cheon,Young Jin Kang,Sook Jae Seo 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

Apolipophorin-III (apoLp-III) is a hemolymph protein whose function is to facilitate lipid transport in an aqueous medium in insects. Recently, apolipophorin-III in Galleria mellonella and Hyphantria cunea was shown to play an unexpected role in insect immune activation. We show here a novel possible function/role of the apoLp-III in insects. To investigate the genes which have a relationship with apoLp-III in fall webworm larvae, we reduced endogenous Hc apoLp-III mRNA levels in larvae via RNA interference (RNAi). The RNAi-mediated Hc apoLp-III reduction resulted in the reduction of antioxidants, like MnSOD, catalase, and glutathione S transferase as well as immune proteins. In particular, expression of MnSOD commonly decreased in fat body, midgut, and hemocytes following the knockdown of Hc apoLp-III, which induced an elevated level of superoxide anion in Hyphantria cunea larvae. The observed effect of Hc apoLp-III RNAi suggests that Hc apoLp-III is related to the action/expression of antioxidants, especially MnSOD.

Comparison of MnSOD and Cu/ZnSOD induction by various stresses from Hyphantria cunea

Yong Il Kim,Hong Ja Kim,Yong Min Kwon,Byung Rae Jin,Young Jin Kang,Hyang Mi Cheon,Nam Gyu Ha,Sook Jae Seo 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Reactive oxygen species (ROS) is toxic to living organisms, because its high reactivity causes oxidative damage to proteins, nucleic acids, and lipids. Superoxide dismutase (SOD) is an enzyme facilitating the removal of superoxide anions from living organisms. This study focused on the cloning of MnSOD cDNA from Hyphantria cuneaand its induction upon bacterial infection and various stresses. The open reading frame of MnSOD is composed of 645 bp, encoding 215 amino acid residues. The theoretical molecular mass and pI of putative MnSOD was evaluated to be 24276 Da and 9.14, respectively. The MnSOD from H. cunea is highly similar to human MnSOD (59.5%) as well as Bombyx mori MnSOD (76.2%). MnSOD showed no big induction upon bacterial infection and stresses, compared to that of Cu/ZnSOD.

Characterization of Hemocyte STAT Gene in Hyphantria cunea

Hong Ja Kim,Young Jin Kang,Yong Min Kwon,Yong Il Kim,In Hee Lee,Byung Rae Jin,Yeon Soo Han,Hyang Mi Cheon,Nam Gyu Ha,Sook Jae Seo 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.05

Innate immunity responses are triggered by the immune challenge and therefore involve signaling processes. The cellular response is initiated by hemocytes and mainly involves phagocytosis and encapsulation of intruders by these cells. To address whether Hc-STAT is activated upon bacterial challenge, we examined the subcellular location of STAT protein in hemocyte by immunostaining. A new insect member of the STAT family of transcription factors (Hc-STAT) has been cloned from the lepidopteran, Hyphantria cunea. The domain involved in DNA interaction and the SH2 domain are well conserved. The gene is transcribed at a low level during all stages of development, and the protein is present in hemocytes, fat body, midgut, epidermis, and Malphigian tuble (Mt). Especially, hemocytes and Mt showed transcriptional activation of Hc-STAT upon Gram (-) bacteria and fungal challenge. Gram (-) bacteria and fungal challenge specifically results in nuclear translocation of Hc-STAT protein and induction of DNA-binding activity that recognizes a STAT target site in H. cunea hemocyte. In vitro treatment with pervanadate translocates Hc-STAT to the nucleus in hemocyte cells. Here we report the first evidence for the involvement hemocyte JAK/STAT pathway upon microbial infection in lepidopteran insect.

Molecular cloning and analysis of the hemocytic STAT gene from Hyphantria cunea

Hong Ja Kim,Young Jin Kang,Yong Min Kwon,Yong Il Kim,Byung Rae Jin,Hyang Mi Cheon,Nam Gyu Ha,Sook Jae Seo 한국응용곤충학회 2008 한국응용곤충학회 학술대회논문집 Vol.2008 No.10

Innate immunity responses are triggered by the immune challenge and therefore involve signaling processes. The cellular response is initiated by hemocytes and mainly involves phagocytosis and encapsulation of intruders by these cells. To address whether Hc-STAT is activated upon bacterial challenge, we examined the subcellular location of STAT protein in hemocyte by immunostaining. A new insect member of the STAT family of transcription factors (Hc-STAT) has been cloned from the lepidopteran, Hyphantria cunea. The domain involved in DNA interaction and the SH2 domain are well conserved. The gene is transcribed at a low level during all stages of development, and the protein is present in hemocytes, fat body, midgut, epidermis, and Malphigian tuble (Mt). Especially, hemocytes and Mt showed transcriptional activation of Hc-STAT upon Gram (-) bacteria and fungal challenge. Gram (-) bacteria and fungal challenge specifically results in nuclear translocation of Hc-STAT protein and induction of DNA-binding activity that recognizes a STAT target site in H. cunea hemocyte. In vitro treatment with pervanadate translocates Hc-STAT to the nucleus in hemocyte cells. Here we report the first evidence for the involvement hemocyte JAK/STAT pathway upon microbial infection in lepidopteran insect.

Comparative analysis of MnSOD and Cu/ZnSOD genes by various stress from Hyphantria cunea

Yong Il Kim,Hong Ja Kim,Yong Min Kwon,Byung Rae Jin,Hyang Mi Cheon,Nam Gyu Ha,Sook Jae Seo 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.05

A MnSOD gene was cloned from the fall webworm, H. cunea. The MnSOD cDNAs encode precursor proteins of 215 amino acid residues. The deduced amino acid sequences of the H. cunea MnSOD cDNA showed 76% identity to B. mori MnSOD and 62-56% to MnSOD sequences from other organisms. MnSOD and Cu/ZnSOD in H. cunea is expressed from all tissues. MnSOD expression is changed at a trace level in infected larvae, while Cu/ZnSOD expression is strongly changed against bacteria, and fungi. The expression level of Cu/ZnSOD increased by different artificial photoperiod (24L:0D), UV irradiation (312nm), and starvation condition, while the expression level of MnSOD only increased by starvation condition. Also, expression of MnSOD and Cu/ZnSOD showed no significant change in 0L:24D condition. In addition to expression levels of Cu/ZnSOD in H. cunea significantly increased by temperature stress and injection with paraquat, but reduced by injection with 10% H2O2. The expression level of MnSOD significantly increased by temperature stress and reduced by injection with 10% H2O2 and paraquat.