Ortho-Chlorobenzylidene Malononitrile(CS) 이 흰쥐 피부조직에 미치는 조직학적 영향에 관한 연구

이우용,최봉근,김잉곤,엄기일,정호삼,정호삼,류재만 한양대학교 의과대학 1998 한양의대 학술지 Vol.18 No.1

This study was undertaken to describe the fine structural changes of cytoplasmic organelles in epidermal cells, and the changes of volume and localization of collagen type Ⅰ in the dermis of rat skin by CS(Ortho-chlorobenzylidene malononitrile) application. The experimental animals, 100∼150gm of body weight rats(Sprague-Dawley strain), were sacrificed at 12 hours, 24 hours, 3 day and 5 day after CS application on the hair-shaved back skin. The specimens were prepared for ultrastructural findings, ultrathin sections were stained with uranyl acetate and lead citrate double stain and observed with Hitachi-600 electron microscrope. The others were incubated with rabbit anti rat type Ⅰ collagen antibody as primary antibody, and Goat anti rabbit IgG antibodies as secondary antibody And advidin-biotin-conjugated(ABC) peroxidase procedure was used. All of specimens for immune reactions were observed with light microscope. The results obtained were as follows. 1 Keratinocytes appeared fully necrotized after 3 days of CS application. The epidermis showed degeneration of basal lamina, enlargement of intercellular spaces and necrotic keratinocytes with vacuole and vesicles. And large keratohyalin granules were seen in the shrunken epidermal cells. 2 At 3 or 5 day after CS treatment, the normal cytoplasmic organelles of keratinocytes began to appear on the basal and prickel cells. 3 At 12 hour an 24 hour after CS treatment. The immune reaction of type Ⅰ collagen showed weak reaction in reticular layer of dermis. But at 3 day and 5 day after CS treatment, moderate immune reaction were seen in throughout the dermis. These results suggest that CS application to rak skin may induce changes of keratinocytes and reductive changes of type Ⅰ collagen in the dermis.

Interleukin-2가 Mouse 비장의 혈구전구체세포에 미치는 영향에 관한 전자현미경적 연구

정호삼 한양대학교 의과대학 1989 한양의대 학술지 Vol.9 No.2

Interleukin-2, T-cell growth factor, is a glycoprotein produced by the lymphocytes and it is widely used as an immunotherapeutic agent on the hepatoma and various carcinoma. Interleukin-2 mediate antitumor effect by stimulating the proliferation of helper and cytotoxic T-cells and increasing the generation of lymphokine activated killer cells. Thus interleukin-2 was administered to the cancer patients and experimental animals, it could also effect on the lymphoblasts and reticular cells in the spleen. In this experiment, the author pursued the effect of interleukin-2 on the ultrastructure of the reticular cell of the mouse spleen. Albino mice, DDY strain, weighing 20gm were used as experimental animals. The experimental animals were killed at 3, 6, 12, 24 and 48 hours after administration of 2,000,000 unit/kg interleukin-2. The specimens obtained from the spleen were prefixed in 2% glutaraldehyde-2.5% paraformaldehyde solution and postfixed in the 1% osmic acid. After dehydration, the specimen were embedded in Epon 812 and then ultrathin sections(600-800A??) were made and stained with urarnyl acetate and lead nitrate. And these preparations were observed with JEM 100cx-II electron microscope. The results were as follows. 1. Medium sized lymphocytes, large lymphocytes and reticular cells were increased in the white pulp of the 3, 6 and 12 hours interleukin-2 treated mouse spleen. A few rod shaped rough endoplasmic reticulum were observed and mitochondria and vesicles were abundant in its cytoplasm. 2. Small lymphocytes, medium sized lymphocytes, large lymphocytes and reticular cells were seen as regular shapes and their cytoplasmic organelles were normal in the white pulp of the spleen in 24 and 48 hour interleukin-2 treated Mice group. Consequently, it is suggested that interleukin-2 would induce the development of the cytoplasmic organelles in the reticular cells but the cytoplasmic organelles in the reticular cells would return to its normal state as the time goes by.

cis-Diamminedichloroplatinum (Ⅱ)이 Hamster 근위곡요세관 상피세포의 미세구조에 미치는 영향

정호삼,이창수,이규식 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.2

cis-Diamminedichloroplatinum (II) (cis-DDP), a metallic compound, similar to bifunctional alkylating agent and has been known as an effective anticancer drug. Cis-DDP is known to inhibit cell division and is so effective to suppress the solid tumors that it has been used in the therapy of acute ovarian tumor, acute epithelial tumor in the neck, bladder cancer and thyroid cancer. cis-DDP reacts with guanine bases of DNA in the cell that it inhibits DNA synthesis, cell division and protein synthesis consequently. It exhibits no specificity with regard to type of cells that it acts on the normal cells as well as cancer cells during therapy. So the author undertook the present study to pursue the effect of cis-DDP on the cytoplasmic organelles of epithelial cell in kidney. Hamster was used as an experimental animals. The experimental animals were killed at 12, 24 and 48 hours after administrations of 6 mg/kg of cis-DDP. The specimen obtained from the renal cortex were fixed and stained with uranyl acetate and lead nitrae. And these preprations were observed with the JEM 100B electron microscope. The results obtained were as follows. 1. The cisternae of rough endoplsmic reticulum were sacculated and membrane bound ribosomes were detached at the epithelium of proximal convoluted tubles in the 24 and 48 hours cis-DDP treated hamster group. 2. Mitochondria were swollen and cristae of mitochondria were not seen at the epithelian cells of proximal convoluted tubules in cis-DDP 48 hours treated hamster group. 3. Vesicles and vacuoles increased in number with time at the epithelium of proximal convoluted tubules in cis-DDP treated hamster groups. 4. Microvilli were segmented at the epithelium of proximal convoluted tubules in cis-DDP 48 hours treated hamster group. Consequently it is suggested that cis-DDP induces the damage in the epithelial cells of proximal convoluted renal tubule.

2인과 3인의 기본 심페소생술 비교

정준영,이창현,김현정,도호석,이삼범,도병수 大韓應急醫學會 1997 대한응급의학회지 Vol.8 No.1

Study objectives: To compare the quality of CPR provided by medical students and pre-EMTs perfoming three-rescuer CPR with that achieved by same students trained to provide standard two-rescuer CPR and to find the most effective method of resuscitation and to educate this methods to students. Material and methods: From July 1 to September 30, 1996, we had trained 60 groups consisted of the medical students, students of EMS technology and pre-EMT. Each group practiced two-rescuer CPR and three-rescuer CPR for 120 minutes with Resusci? Anne and perfomed CPR for at least 3 minutes without feedback. To eliminate any visual cues of perfomance the mannequin's recorder was hidden. Each actions were recorded by Resusci? skillmeter and Laerdal printer. To compare the quality of two-rescuer CPR and three-rescuer CPR, we analyzed the data by SPSS with paired t-test. Results: Three-rescuer teams delivered a mean minute ventilation and a mean minute cardiac compression substantially greater than that produced by two-rescuer teams(11.30±1.85 vs 9.09±2.22, p<0.001; 99.22±12.42 vs 95.12±15.75, p<0.001). Three-rescuer teams produce the more correct actions in chest compression and ventilation(93.84±7.56% vs 83.49±16.94%, 90.90±9.33% vs 77.00±17.06%). Conclusion: Three-rescuers can produce better CPR than two rescuers when a bag-valve-mask devices is used and the technique is easily learned and readily retrained, so we think that repeated education and training of this CPR methods to students is needed.

흰쥐비장내 백수모세혈관의 기원과 분화

鄭鎬三 건국대학교 1977 論文集 Vol.6 No.1

Problems of the follicular capillaries and the marginal sinuses in the white pulp of the spleen have long been a matter of general interest in relation to the production of the antibody in the spleen. It has been reported that the histologists in the field of the spleen studies (Altschul and Hummason '47, Snook '50 '64, Andrew '46, Galindo and Imaeda '62, and Weiss. '73) have defined that the follicular capillaries are branched from the central arteries of the white pulp and that the blood from the central arteries are supplied into the germinal center and marginal zones. As follows, a series of different results have been obtained in the present study of the developing spleen tissue of the neonatal albino rats. 1.The capillaries from the cetral artery in the neonatal rat spleen did not branch within 13 days after birth. 2.At the stage of the 3rd day after birth, the follicular capillaries began to branch from the penicillar arterioles in the zone between the periphery of the white pulp and red pulp. 3.At the stage of 3rd day after birth, the reticular fibers in the white pulp began to form; and at the stage of 7th day after b]rth, a reticular fiber formation was completed almost as that of the adult rat. 4.At the stage of 14th day after birth, the central artery began to locate peripherally in the white pulp : and the germinal center was formed completely. 5.At the stake of 14th day after birth, the formation of the marginal zone and marginal sinuses was completed: and the follicular capillaries and marginal sinuses began to anastomose.

白鼠의 脛骨 骨端軟骨板의 分化에 관한 硏究

鄭鎬三,吳正一,李圭植 한양대학교 의과대학 1984 한양의대 학술지 Vol.4 No.1

It was well documented that in the course of development of rat tibia in formation of cartilage model from the mesenchymal cells and subsequent ossification generally starts as early as 15-16th day of embryonic stage and that development of tibia completes by the 8-10th day after birth. Rather extensive studies have been made on the differention of cartilage cells, including their secretory activities of matrix and composition of matrix as well. However, there are still much to be clarified on the more precise feature of differentiation and growth of epiphyseal plates in the rat tibia. Therefore the present study was designed to explore differentiation of epiphyseal plate biochemically and histologsically at each developing stage of rat tibia. The albino of Wistar strain were used as experimental animal. And expreimental animals were sacrificed at 16, 18, 20th of fetal life and 2nd, 4th and 10th day of neonatal life. The epiphyseal plates were removed from tibia and were fixed in 10% formalin and 2% glutraldehyde-2.5% paraformaldehyde phosphate. The PAS-hematoxylin stain was carried out for the study of general development of tibia and alcian blue (1.0), alcian blue (2.%) and aldehyde fuchsin-alcian blue stains for the determination of mucopolysaccharide variations. Also, thin sections were prepared and stained with uranyl acetate and lead citrate stain for the ultramicroscopic study of chondrocytes and matrix of epiphyseal plates at different stages of development of rat tibia. The results obtained were as fokkows: 1. A small amount of acid unsulfated mucin was found in the matrix of epiphyseal plate as early as 16th day of fetal life. 2. On the 18th day of fetal life existance of acid mucin and sulfated mucin were recognized in the matrix of epiphyseal plate and thereafter they were increased in amount. 3. The acid mucin reached maximum level at 2nd day of neonatal life and thereafter sulfated mucin gradually appeared in the mathix of epiphyseal plate. 4. In the proliferative zone of tibia of 18day old embryo, secretary granules were deposited in the all membranes of chondrocyte. As the fetus grew, the namber and size of secreted granules were increased approprocial by increase in salfated mucin: And the rough endoplasmic reticulum in the chondrocytes was increased also. To summerize above results the acid mucin start to appear in the matrix of epiphyeal plate as ealry as 16th day of fetal life and later a part of acid mucin is changed to neutral sufated mucin, the secretary granules observed on the cell membrane of chondrocytes of 12 day old embryo seems to be sulfated mucin.

L-Asparaginase가 Mouse의 肝細胞에 미치는 影響

鄭鎬三,李圭植,尹康默 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.1

L-asparaginase hydrolysis L-asparagine which is essential amino acid for tumor cells to produce an antitumorous effect. On the other hand, it was reported that this drug provoked cytotoxic effect following the inhibition of protein synthesis and that this was a consequence of the exhaustion of L-asparagine in the normal cell. Therefore, the authors have demonstrated the toxicity of L-asparaginase on the hepatic parenchymal cell observing with light and electron microscope: Male mice of ICR strain were used in this experiment and the weight of experimental animals was around 20gm. The animals were divided into two groups, the control and the L-asparaginase treated groups and subdivided into the 6th, 12th and 24th hours groups. L-asparaginase 2,500 I.U./kg, in the water for injection was injected intraperitoneally for the animals of L-asparaginase treatment group. The animals of control group were given water for injection only. The experimental animals were sacrificed at the 6,12 and 24 hours after an administration of L-asparaginase or water for injection. The liver specimens were stained with hematoxylin oesin, methyl green-pyronin and oil red 0 method and observed them under the light microscope. And the ultrathin sectioned specimens were stained with uranyl acetate and lead citrate and observed with the JEM 100B electron microscope. The results obtained were as follows: 1. Cellular swelling accompanied with the vesicular or vacuolar formation, reduction or disappearance of the pyroninophilic granules and fat infiltration were observed in the hepatic parenchymal cells of the L-asparaginase treated mouse. 2. Dilatation, sacculation and fragmentation of the cisternae of RER were accompanied with dissociation of membrane bound ribosomes, and proliferation of SER associated with depletion of glycogen particles were observed. And formation of numerous primary lysosomes, secondary lysosomes, autophagic vacuoles, multivesicular bodies and irregular sized lipid droplets were recognized in the hepatic parenchymal cells of the L-asparaginase treated mouse. Consequently, it is suggested that intraperitoreal injection of L-asparaginase would include the cellular degeneration of the hepatic parenchymal cells in the mouse.

장 폴 고티에 (Jean-Paul Gaultier)가 현대 패선에 준 영향에 관한 연구 : 아방가르드와 표현주의 개념을 중심으로 Based on Avantgarde and Fantastic Expressionism

정삼호,최은진 중앙대학교 생활과학연구소 2003 생활과학논집 Vol.17 No.-

This cultural change greatly helped to create an avant-garde fashion style which is characterized by nonconformity and diversity. Therefore, to study the most noticeable forerunner in avant-garde fashion style, Jean Paul Gautier, and his works will be equivalent to having insight on the most important trend of modern fashion, besides it will clarify his influence on modern fashion and strategy for his brand popularization. Because the aesthetic characteristics of Gautier fashion can be explained as expressionism, a study on the modern fashion in view of expressionism will be performed and the aesthetic value of Gautier fashion will be found in modern fashion trend from aspects of exotism, sexual liberalization, and fantastic grotesqueness. Gautier's exotism is to adopt exotic techniques from different fashion including traditional clothes, anachronistic style, and plebeian culture. Fantastic grotesqueness of Gautier fashion is lying on his design concept which creates an silly and humourous ambience, and it relinquishes all regularity to gain eccentricity. In this paper, avant-garde characteristics of Gautier fashion will be categorized into nonconformity, sexual image, and combination of heterogeneous things. Avant-garde disconnects itself completely from all existing customs and traditions, for it can be called as 'anti-art for anti art'. Creating genderlessness in clothes, challenging any existing prejudice on models, and radical design overthrowing traditional clothing shows nonconformity. And turning underwear out and using delicate religious theme in fashion show indicates his desire for challenging any taboo. Avant-garde style will lead future fashion trend by various techniques and features adopting many heterogeneous fashion styles adapted to itself.

Malathion이 Mouse 肝細胞의 微細構造에 미치는 影響

鄭鎬三,李圭植,金振宅 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.1

Malathin (0,0-dimethyl 5-[1,2 dicarboethoxyethyl] dithiophosphate) is one of the organic phosphorus compounds and has been widely used as an insecticide. It is well known that the organophosphorus compounds inactivate an enzyme, cholinesterase, and resultant accumulation of acetylcholine effects on a pattern of cholinergic stimulation. Present study has demonstrated the hepatotoxicity caused by malathion, an organophosphorus compound, observing the ultrastructural changes in the cytoplasmic organelles of hepatocytes in mice. The experimental animals were given 0.2ml of malathion diluted in olive oil(100mg/kg) by intraperitoneal injection. The animals of control group were killed at 6 hours after olive oil treatment, and the animals of the experimental groups were killed at 12, 24 and 36 hours after administration of malathion, respectively. The specimens obtained from the left anterior lobes of the liver were stained with uranyl acetate and lead citrate double contrast methods and observed with JEM 100B model electron microscope. The cytoplasmic organelles of hepatocyte in malathion trated mice showed the fallowing significant changes. 1) A slight morphological changes such as dilatation, sacculation and fragementation of the cisternae of rough endoplasmic reticulum associated with detachment of membrane-bound ribosomes and disaggregation of the free ribosomes are recognized. 2) Primary lysosomes were increased in number and formation of secondary lysosomes (autophagic vaculoes and residual bodies) were observed. 3) The smooth endoplasmic reticulum associated with depletion of the glycogen particles were noted. 4) Large amount of lipid droplets were originated. The results obtained in the present study suggested that malathion appeared to by cytotoxic to the liver cells of mice and that the organophosphorus compound would induce retrograde changes characterized by destruction of small organelles of the hepatocytes.

5-Fluorouracil이 흰쥐 肝臟의 燐酸鹽 分解酵素의 活性에 미치는 影響

鄭鎬三,蘇秉造,李圭植 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.1

The 5-Fluorouracil (5-FU) has been used as a synthetic antineoplastic agent with the synthesis of nucleic acid in the cell. Unfortunately this drug is known to cause toxic reaction in normal tissue also. In this experiment, the authors studied the effect of 5-FU on the rat liver histochemically observing the change in the activities of acid phosphatase and adenosine triphosphatase. Healthy albino male rats (Wistar strain) were used as experimental animals which were divided into the control and 5-FU treated groups. All experimental animals were given food and water unlimited. The animals of experimental group were administered 5-FU, in a dose of 50mg per kg of body weight by intraperitoneal injection. The animals of control groups were administered only water for injectio. The experimental animals were sacrificed at 6, 12, 24 and 36 hours after administration of 5-FU, respectively. The liver specimens, sectioned by cryostat were stained by Gomori method and by Wachestein and Meisel method for studying changes in the activities of acid phosphatase and that of ATPase, respectively. The results obtained were as follows: 1. The acid phosphatase activity was increased in the hepatic parenchymal cells of the 5-FU treated rat liver. 2. The adenosine triphophatase activity was decreased in the intralobular bile canaliculi of the 5-FU treated rat liver. Consequently, it is suggested that 5-FU would induce the change in the activities of possibly from production of toxic metabolites and subsequent damage in rat liver.