Loss of cytosolic fructose-1,6-bisphosphatase limits photosynthetic sucrose synthesis and causes severe growth retardations in rice (<i>Oryza sativa</i>)

LEE, SANG-KYU,JEON, JONG-SEONG,BÖ,RNKE, FREDERIK,VOLL, LARS,CHO, JUNG-IL,GOH, CHANG-HYO,JEONG, SUK-WON,PARK, YOUN-IL,KIM, SUNG JIN,CHOI, SANG-BONG,MIYAO, AKIO,HIROCHIKA, HIROHIKO,AN, GYNHEUNG,CHO, Blackwell Publishing Ltd 2008 Plant, cell and environment Vol.31 No.12

<P>ABSTRACT</P><P>During photosynthesis, triose-phosphates (trioseP) exported from the chloroplast to the cytosol are converted to sucrose via cytosolic fructose-1,6-bisphosphatase (cFBPase). Expression analysis in rice suggests that OscFBP1 plays a major role in the cytosolic conversion of trioseP to sucrose in leaves during the day. The isolated <I>OscFBP1</I> mutants exhibited markedly decreased photosynthetic rates and severe growth retardation with reduced chlorophyll content, which results in plant death. Analysis of primary carbon metabolites revealed both significantly reduced levels of sucrose, glucose, fructose and starch in leaves of these mutants, and a high accumulation of sucrose to starch in leaves of rice plants. In the <I>oscfbp1</I> mutants, products of glycolysis and the TCA cycle were significantly increased. A partitioning experiment of <SUP>14</SUP>C-labelled photoassimilates revealed altered carbon distributions including a slight increase in the insoluble fraction representing transitory starch, a significant decrease in the neutral fraction corresponding to soluble sugars and a high accumulation of phosphorylated intermediates and carboxylic acid fractions in the <I>oscfbp1</I> mutants. These results indicate that the impaired synthesis of sucrose in rice cannot be sufficiently compensated for by the transitory starch-mediated pathways that have been found to facilitate plant growth in the equivalent <I>Arabidopsis</I> mutants.</P>

불로환(不老丸)을 투여한 흰쥐 뇌의 항산화효과에 관한 연구

박진성,고성규,이선동,Park Jin-Sung,Goh Seong-Kyu,Lee Sun-Dong 대한예방한의학회 2001 대한예방한의학회지 Vol.5 No.1

Objectives: Resently Oxidative stress of brain was proved the cause of Alzheimer and stroke sequel. It has important significance in prevention and treatment of cerebropathia that Bulnohwan used as formula of senescence delay have antioxidative effect. The purposes of this study is to investigate the effect of Bulnohwan on antioxidant defense systems such as thiobarbituric acid reactive substances(TBARS), Superoxide dismutase (SOD), Catalase (CAT), Glutathione peroxidase (GSH-PX), Glutathione S-transperase (GST), Glutathione (GSH) in rat brain. Method: Sprague - Dawley rats were divided into 3 groups; saline solution administered control group, Bulnohwan extract administered Experimental group I and Bulnohwan adminisrtrated, 40% dietary restricted Experimental group II. Animals were sacrificed at 12 weeks after treatment TBARS, SOD, CAT, GSH-PX, GST and GSH were measured in mts brain. Results: weight of brain was no stastical significance.(p>0.05) TBARS contents were significant decrease in Experimental group I, II. (p<0.001) SOD activity was stastical significance in Experimental group II, whereas it was no stastical significance Experimental group II.(p<0.0001) Catalase activites were significant increase in . (p<0.00l) Glutathione Peroxidase activites were significant increase in Experimental group I,II. (p<0.000l) Glutathione S-transferase activites were significant increase in Experimental group I, II. (p<0.000) However there were no statistical significance each other. Glutathione contents were significant increase in Experimental group I, II. (p<0.00l) Conclusions: According to the above results, it is considered that Bulohwan has antioxidants effect in rat brain. When Bulohwan goes with diet restriction, there has more Antioxidants effect in rat brain. but this study was perfored retrospectively. So more prospective studies about mutual relation of drugs are needed

형광등에서의 발화원인 감정사례

김윤희,박남규,박종찬,고재모,장성길,정진태 내무부 국립과학수사연구소 1994 國立科學搜査硏究所年報 Vol.26 No.-

오적산(五積散)을 투여한 흰쥐의 뇌중 금속농도변화에 관한 연구

민대기,고성규,이선동,문용,Min Dae-Ki,Goh Seong-Kyu,Lee Sun-Dong,Wen Yong 대한예방한의학회 2000 대한예방한의학회지 Vol.4 No.2

This study was to investigate the metal accumulation from Ohjeoksan-Decoction to rat brain(Sprague Dawley). 1. In Control group, each metal concentration was within 26.65mg/kg. and in each experimental groups, was within 28.39mg/kg. And there were no significant metal concentration between control and experimental groups. Exceptionally, Cr and Ni level of control group was lower than experimental groups significantly. (p<0.05) and Cr and Ni level of experimental I group was lower than other experimental groups too. (p<0.05). But, Pb level of control group was higher than experimental groups significantly (p<0.05) Pb level of experimental I group was higher than other experimental groups(p<0.01) 2. In non-hazardous, hazardous and total metal concentration, there was no increase tendency in brain according to the high dose of OD(Ohjeoksan-Decoction) intake. Reversely, in experimental groups, hazardous metal concentration was decrease by high OD intake. (P<0.05) 3. Correlation among each metal in brain was various in each groups Briefly under the intake of Ohjeoksan-Decoction, that if very busy herb prescription , this study was defined within safety in brain metal level by P.O. during 10 days. But, there should be a more research about Pb increase by high dose OD intake

Skin Safety Evaluation of Long-Term treatment of an Electrolyzed Neutral Oxidizing Water on C57BL/6 mice

Md. Habibur Rahman,Ailyn Fadriquela,Johny Bajgai,Seong-Hoon Goh,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Skin is a complex barrier organ that can fight against various endogenous and exogenous factors. The damaged skin barrier can lead to different skin problems that can exhibit the skin condition such as a decrease of elasticity and sebum level, an increase of water loss, strengthening of keratinocyte cohesion and wrinkle formation, which will eventually lead to different skin diseases. With the current use of chlorine-rich water as disinfectant and its potential in medical field, it is important to check its safety use. The objective of the study was to evaluate skin safety of electrolyzed neutral oxidizing water (ENOW) (pH: 7.11, oxidation reduction potential: 450 mV, available chlorine concentration: 5.0 ppm) after the long-term use (90-day skin application) on the skin of C57BL/6 mice compared to normal control group (NC) treated with tap water (TW) (pH: 7.8, oxidation reduction potential: 500mV, available chlorine concentration: 0.7 ppm). We were measured skin state such as the moisture, elasticity, sebum, pore size, and wrinkle. Oxidative stress markers such as reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxides (GPx) and catalase (CAT) were measuredin using serum and skin lysate samples. Inflammation cytokines such as IL-1α, IL-1β, IL-13, GM-CSF and TNF-α, and the Th1/Th2 cytokines such as IL-2, IL-4, IL-5, IL-6, IL-10 and IL-12 were also analyzed. In consequently, The skin state of ENOW group showed higher level of elasticity and moisture compared to NC group. ROS and NO levels in skin did not show adverse effect in ENOW group. GPx were also controlled in all groups, however, a significant decrease in CAT level was observed in both NC and ENOW groups. Moreover, serum inflammatory cytokine of ENOW group did not show significant difference as compared to NC and TW groups, however, GM-CSF showed a significantly lower level in the ENOW group than NC group. Cytokines related to allergic response showed no difference in the skin. Taken together, long term treatment of ENOW might be safe on skin in vivo . However, more clinical studies are needed to verify the skin safety of ENOW.

The Effect of a Granule-type Anti-hangover Compound, Quechung, on Acute Alcohol-induced Hangover in Healthy Subjects : a Randomized Crossover Study

Subham Sharma,Johny Bajgai,Ailyn Fadriquela,Md. Habibur Rahman,Trinh Thi Thuy,Seong-Hoon Goh,Cheol-Su Kim,Kijong Yu,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

About two billion people in the world drink alcoholic products and among them, more than 76 million are suffering from serious alcoholic disorder including hangover. Hanover can be caused by the by-products which are produced in the process of alcohol metabolism such as acetaldehyde and acetate in human body. There are various type of anti-hangover-related commercial products which can help to somewhat reduce hangover symptoms. In this study, we investigated the effects of Quechung which is a granule-type of anti-hangover compounds containing functional substances such as HK mycelial culture of Lentinus edodes, Hovenia dulcis fruit extract, Lactobicillus fermentum MG590 and so on. The subjects were 20 healthy men and women, and washout period for the crossover design was 7 days. Participants were randomly divided into two groups: the control group (alcohol + purified water) and the experimental group (alcohol + Quechung). The experimental group was first supplied with the anti-hangover compound before and after 30 min of 20% alcohol intake (7.8 g/kg body weight). Blood pressure, temperature, heart rate, and alcohol concentration in breath and blood were measured one time before alcohol intake and also 3 times after Quechung intake at an interval of 1 h. Biochemical parameters such as albumin, electrolytes, lipid profile, liver function markers, alcohol level in breath and blood, acetaldehyde concentration were analyzed. Consequently, breath alcolol concentration was lower in the experimental group than control group after 1 h, especially showed statistical difference in female. Blood alcohol concentration before and after treatment also showed significant decrease in experimental group, whereas blood acetaldehyde showed significant increase in control group. Especially female participants showed higher effect than male. In the reslut of AST, experimental group showed little difference before and after treatment but control group showed significant increase, in addition female much higher effect than male. Taken together, this anti-hangover compound, Quechung, clinically showed the hangover-relieving effect on the acute alcohol-induced hangover symptom based on the positive results of alcohol concentration, acetaldehyde concentration, and level of liver function markers etc. in addition, Quechung also can influence on the protection of liver and blood vessel. This clinical study has a significance in that it provides scientific evidence to systemically establish database for the effectiveness and efficiency of the commercial anti-hangover products, and to help consummer’s easy choice and their better health.

Redox Effects of Molecular Hydrogen in C57BL/6 Mouse Model with Trimethyltin-induced Cognitive Dysfunction

Johny Bajgai,Md. Habibur Rahman,Ailyn Fadriquela,Subham Sharma,Dong-Heui Kim,Seong-Hoon Goh,In-Soo You,Yun-Su Na,Nam-Kyu Kong,Soo-Ki Kim,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Various neurodegenerative diseases including dementia and cognitive dysfunction pathogenesis are caused due to oxidative stress. Several studies have proved that molecular hydrogen (H2) acts as a therapeutic and preventive anti-oxidant, however, H2 effect on dementia is poorly documented. In the present study, we investigated the effects of H2 inhalation on trimethyltin (TMT)-induced memory dysfunction in the C57BL/6 mice. For this, mice were intraperitoneally injected a single dosage of TMT 2.6 mg/kg body weight. The subjects were randomly divided into the following groups: normal control without TMT induction, TMT only as a negative control (TMT injection + saline administration), positive control group [TMT injection + lithium chloride (50 mg/kg body weight) injection], H2-2wk group [TMT injection + H2 gas inhalation (once a day for 30 min for 2 weeks) and H2-4wk group [TMT injection + H2 gas inhalation (once a day for 30 min for 4 weeks). As a result, TMT-exposed mice showed symptoms such as tremor, seizure and aggressive behavior. Meanwhile, H2-treated groups after TMT treatment showed significantly increased spatial cognition abilities in the Y-maze test. Likewise, the level of the oxidative stress markers such as ROS, NO and MDA were found to be decreased in H2-2wk and H2-4wk groups as compared to the TMT only group in both serum and brain lysate, coherently, antioxidant enzymatic activities were increased in H2-2wk and H2-4wk groups compared to TMT only group. Moreover, H2 showed prevention effect of neuronal apoptosis through the suppression of apoptotic signaling molecules such as BAX, and cleaved caspase-7. Overall, H2 inhalation exhibited memory enhancing activity and decreased the oxidative stress markers and suppressed apoptotic signaling molecules on TMT-inducd mice. Therefore, H2 might be one of candidates for repairing dementia and neuro-degenerative diseases which shows symptoms such as dysfunction of learning, memory and cognition. However further studies are needed to clarify this notion.

Safety Evaluation of Drinking Mineral Supplement Hypochlorous Acid Water on Helicobacter pylori-Infected C57BL/6 Mouse Model

Md. Habibur Rahman,Johny Bajgai,Ailyn Fadriquela,Subham Sharma,Trinh Thi Thuy,Seong-Hoon Goh,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Hypochlorous acid water can promote pruritus as an adverse effect under certain conditions, which should be taken into account to ensure safety. In recent days, Mineral Supplement Hypochlorous Acid Water (MS-HOCl) has received massive attention in research as a new-concept cleansing agent due to its high sterilizing properties, easy accessibility, and safety aspects. This present study was conducted to evaluate the drinking effects of MS-HOCl treatment of the Helicobacter pylori (H. pylori )-infected C57BL/6 mouse model to verify the safety effects. The experimental mice were divided into 3 groups: NC (Purified water + no infection), PW (Purified water + H. pylori infection), and MS-HOCL (MS-HOCl + H. pylori infection). Water was supplied through water bottle for mouse for 4 weeks, and changed once a day. We measured the bodyweight, and total WBC and its differential counts such as lymphocytes, monocytes, neutrophil, eosinophil and basophil. Besides that, we also tested biochemical markers such as reactive oxygen species (ROS), nitric oxide (NO), glutathione peroxides (GPx), and calcium activity in both serum and stomach lysate samples. Our results showed that daily oral administration of MS-HOCl water for 4 wk did not show any abnormal findings about hematological and biochemical parameters such as ROS, NO, GPx, and calcium activity in both serum and stomach lysate. There were no significant differences in bodyweight between the three groups. Overall, our results showed no negative effects of drinking MS-HOCl in the H. pylori -infected C57BL/6 mice model. However, more controlled studies are needed to determine the safety of drinking MS-HOCl.

차아염소산수 공중 분무를 통한 인체 병원성 황색포도상구균 살균효과 평가

Md. Habibur Rahman,Johny Bajgai,Ailyn Fadriquela,Subham Sharma,Trinh Thi Thuy,Goh Seong Hoon,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Hypochlorous acid has very ideal condition such as powerful and broad sterilizing effect, easy accessibility, low price and high safety as a disinfectant. It can be applied by various ways, but the effect may vary depending on the method, so it is necessary to establish use standard for the maximized effect. In this study, we investigated the sterilizing effect of hypochlorous acid indoor environments contaminated with Staphylococcus aureus using fogging method. Hypochlorous acid water was classified two: Huureka-E generated by electrolysis [pH 5.4, oxidation-reduction potential (ORP) 530, active chlorine concentration (ACC) 51], and Huureka-M produced by a mixing and dilution method (pH 5.5, ORP 913, ACC 53). Blood agar plate were put on the table 1 m high in an enclosed room, then hypochlorous acid was fogged up to 80% humidity, and S. aureus-diluted solution was sprayed three times on the air. The plate was moved for bacterial culture 0 min 30 min, 60 min, and 90 min after spraying bacteria. As a result of the bacterial culture, the growth inhibitory rate of Huureka-M was 92.2 ± 0.8%, 96.0 ± 0.6% and 97.4 ± 0.1% time-dependently (p < 0.001 vs. 0 minm, respectively), and that of Huureka-E was 66.1 ± 1.3%, 76.4 ± 2.4% and 98.2 ± 0.6% (p < 0.001 vs. 0 min, respectively). In confluence, the fogging of both electrolysis-type and mixing-type hypochlorous acid water showed highly satisfactory sterilizing power as a disinfectant. Since spraying or fogging is a highly effective application method for a large space, hypochlorous acid can be recommended to be utilized in the hospital, agriculture sector, livestock industry, public facilities, etc. Further study will be necessary to be standardized the sterilizing protocol according to the application method and ACC.

Application of Hydrogen for the Health Improvement in Vietnam

Trinh Thi Thuy,Johny Bajgai,Md. Habibur Rahman,Ailyn Fadriquela,Subham Sharma,Goh Seong Hoon,Cheol-Su Kim,Kyu-Jae Lee 한국물학회 2020 한국물학회지 Vol.8 No.1

Health and vibrant life are required by everyone around the globe. To improve quality of life, maintaining a healthy state, and to prevent various diseases, and to evaluate the effects of potentially improve quality of life increasing factors are important. In recent years, oxidative stress has been postulated to be an important factor in the pathogenesis and development of lifestyle-related diseases, cancer, and the aging process. Despite the clinical importance of oxidative damage due to oxidative stress, antioxidants have been of limited therapeutic success. Of these, molecular hydrogen (H2) is known as a “novel” antioxidant in preventive and therapeutic applications. H2 shows not only effects against oxidative stress but also various anti-inflammatory and anti-allergic effects. Thus, H2 is nowadays used in daily life as well as and in medical field in both developed and developing countries such as Japan, USA, and China. Unfortunately, in Vietnam, one of the developing countries in the world has a poor health system and a high death rate due to non-communicable diseases like cancer, cardiovascular, and diabetes. Growing evidence has shown that hydrogen gas can either alleviate the side effects caused by conventional chemotherapeutics, or suppress the growth of cancer cells and xenograft tumor, suggesting its broad potent application in clinical therapy. Besides that studies have reported that H2 also has effects of on diseases such as diabetes, cerebral infarction, and Alzheimer’s disease. However in Vietnam, currently there are only a few research related to H2 has conducted that mainly focus on the application in the environment, energy industry. Therefore, H2 in Vietnam in medical field needs to be exploited in the future as it is completely new area.