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Yang Yu,Qi-Gai Yin,Li-Jing Ye,Hui Yu 한국고분자학회 2021 Macromolecular Research Vol.29 No.9
Via applying a dual-functional tetrazolyl-carboxylic ligand 5-(1H-tetrazole- 5-yl)-1,3-bis(3,5-dicarboxylphenyl)-benzene (H5TBDPB), a new Zn(II)-containing metalorganic framework (MOF) with the chemical composition of [Zn6(HTBDPB)3(H2O)6]∙ 9DMF∙15H2O (1) has been successfully prepared. This article methodically explored the gas sorption characteristics of activated 1 for C2H2, CO2, and CH4, and it holds a relative large C2H2 sorption capacity of 164.0 cm3 (STP) g-1 at 298 K and 1 bar with the C2H2/CH4 and C2H2/CO2 sorption selectivity of 34.8 and 4.6 at 298 K, respectively, revealing its potential application for the selective C2H2 adsorption. Additionally, we studied its application value on the infantile meningitis as well as the related mechanism was evaluated. The results of the bacteria growth curve automatic analysis indicated the compound could decrease the growth of Streptococcus meningitidis bacteria. Besides, the compound could also inhibit the survival genes expression in Streptococcus meningitidis in a dose-dependent manner. Molecular docking simulation has revealed that the carboxyl groups are the donor for the formed hydrogen bonds, while the tetrazole groups are the acceptor for the formed hydrogen bonds, such features make the Zn complex as an attractive candidate for infantile meningitis treatment.
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Mapping QTLs for Tissue Culture Response in Soybean (Glycine max (L.) Merr.)
Chao Yang,Tuanjie Zhao,Deyue Yu,Junyi Gai 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.4
Quantitative trait loci (QTLs) that control the tissue cul-ture response in soybean were detected by using 184 recombinant inbred lines (RILs) derived from two varieties: Kefeng No.1 and Nannong 1138-2. The molecular map consisting of 834 molecular markers using this population covered space 2307.83 cM of the genome throughout 24 linkage groups. The performance of tissue culture in soybean was evaluated by two indices: callus induction frequency (CIF) and somatic embryos initiation frequency (SEIF). They were expressed as the number of explants producing callus/ the number of total explants and the number of explants producing somatic embryos/ the number of total explants, respectively. The RIL lines showed continuous segregation for both indices. With the composite interval mapping (CIM) described in Windows QTL Cartographer Version 2.5, three quantitative trait loci (QTLs) were identified for the frequency of callus induction, on chromosomes B2 and D2, accounting for phenotypic variation from 5.84% to 16.60%; four QTLs on chromo-some G were detected for the frequency of somatic em-bryos initiation and explained the phenotypic variation from 7.79% to 14.16%. The information of new QTLs identified in the present study will contribute to genetic improvement of regeneration traits with marker-assisted selection (MAS) in soybean.
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Li-Fei Yang,Jun-Yi Gai,Yue-Lin Zhu,Gang Chen,Guo-Ping Wei,Cong Wang,Qian-Qian Liu 한국원예학회 2009 Horticulture, Environment, and Biotechnology Vol.50 No.1
The objectives of this paper were to construct a full-length cDNA library and identify genes encoding high quality proteins in developing seeds of vegetable soybean [Glycine max (L.) Merr.]. The construction of full-length cDNA library was essential for screening and cloning of novel genes. Using vegetable soybean variety ‘NN99-10’ as an experimental material, a full-length cDNA library from the developing seeds was constructed by switching mechanism at 5’-end of RNA transcript (SMART) approach. Total RNA from different developmental stages of seeds was purified. Double strand cDNA was synthesized by SMART method and the cDNA fragments were ligated to the pBluescript II SK vector. The recombinant plasmids were transformed into E. coli by electroporation and a cDNA library of developing seeds was successfully constructed. By counting the number of colonies, the titer of the primary cDNA library was 7.6×10? cfuㆍ㎖<SUP>-1</SUP>, and that of the amplified library was 2.4×10<SUP>12</SUP> cfuㆍ㎖<SUP>-1</SUP>. Electrophoresis gel results indicated that most of the cDNA inserts were ranged from 0.40 to 2.0 kb, with an average size of larger than 800 bp. Randomly picked clones showed that the recombination rate was above 99%. These evidences showed that the cDNA library constructed was a full-length library with high quality, and could be used to screen the genes encoding high quality proteins. A total of 206 randomly selected positive clones were successfully sequenced, and 73% of them contained full-length coding regions. Based on bioinformatics analysis of the sequences, we obtained 192 expressed sequence tags (ESTs) from 206 cDNA positive clones. Eight important genes which might be involved in seed quality were identified, and two of their genes were identified to be related to the allergenicity of seed proteins. The full-length cDNA library constructed in this research has laid the foundation for the further cloning of novel genes and the revealed EST sequences have enriched our knowledge of the function genes of vegetable soybean seeds.
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Hu Yancai,Gai Xudong,Zhang Qiang,Liu Yang 국제이네비해양경제학회 2023 International Journal of e-Navigation and Maritime Vol.20 No.-
Ship collision accidents not only endanger the safety of ships and personnel, but also may cause serious marine environmental pollution. To solve this problem, advanced technologies have been developed and applied in the field of intelligent ships in recent years. In this paper, a novel path planning algorithm is proposed based on particle swarm optimization (PSO) to construct a decision-making system for ship's autonomous collision avoidance using the process analysis which combines with the ship encounter situation and the decision-making method based on ship collision avoidance responsibility. This algorithm is designed to avoid both static and dynamic obstacles by judging the collision risk considering bad weather conditions by using BP neural network. When the two ships enter a certain distance, the optimal collision avoidance course and speed of the ship are obtained through the improved collision avoidance decision-making method. Finally, through MATLAB and Visual C++ platform simulations, the results show that the ship collision avoidance decision-making scheme can obtain reasonable optimal collision avoidance speed and course, which can ensure the safety of ship path planning and reduce energy consumption.
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An improved particles model for stress sensitivity in low-permeability sandstones
S. H. Gai,H. Q. Liu,S. L. He,G. Lei,S. Y. Mo,X. Huang,Y. Yang,X. Chen 한국자원공학회 2016 Geosystem engineering Vol.19 No.2
Stress-dependent permeability and porosity of porous media is very important for the development of oil reservoirs, especially in low-permeability reservoirs. Porous structure can change in response to an increase in the effective stress to induce the decline of permeability and porosity, which ultimately affects the fluid flow property. In this study, a capillary module, considering the arrangement and the deformation of particles, has been established to express the stress sensitivity of porous media based on the particle packing model. The predictions of variation of permeability by the proposed model have been validated by comparing with the experimental data. The predictions by the current model show the same variation trend with the experimental data and are consistent with the compression experiments. The proposed variation of permeability with the increase in effective stress depends on the factors such as the arrangement of particles (contact angle), the deformation of particles (elastic module and Poisson’s ratio) and effective stress. The effects of these parameters on variation of permeability are discussed in detail.
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Jiajia Li,Shouping Yang,Junyi Gai 한국유전학회 2017 Genes & Genomics Vol.39 No.10
To further elucidate the molecular mechanism and fertility restoration of cytoplasmic male sterility (CMS) in soybean, a comparative transcriptome analysis was conducted between the CMS line NJCMS1A, restorer line NJCMS1C and their hybrid F1 progeny (NJCMS1A × NJCMS1C) using RNA-Seq strategy. After pairwise comparative analysis of these soybean lines, 294, 222, and 288 differentially expressed genes (DEGs) were identified, respectively. Further bioinformatic analysis indicated that these DEGs were involved in diverse molecular functions and metabolic pathways. qRT-PCR analysis validated that the gene expression pattern in RNA-Seq was reliable. These results significantly showed that the male sterility and fertility restoration in NJCMS1A might be related to a series of the abnormal of growth development and metabolic processes, such as pollen development, DNA methylation process, pollen viability, cell wall development, programmed cell death, as well as carbohydrate and energy metabolism. This study could facilitate our understanding of the molecular mechanisms and fertility restoration behind CMS in soybean.
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Gang Chen,Hua Wang,Jun-Yi Gai,Yue-Lin Zhu,Li-Fei Yang,Qian-Qian Liu,Gong-Chen Zhang,Guo-Hu Chen 한국원예학회 2012 Horticulture, Environment, and Biotechnology Vol.53 No.2
The objectives of this paper were to construct a full-length cDNA library and to isolate genes that confer salt tolerance from the leaves of salinity-tolerant wild eggplant variety, ‘Torvum Vigor’ (Solanum torvum Swartz). A full-length cDNA library from the leaves was successfully constructed by a switching mechanism at 5’-end of RNA transcript (SMART) approach and a long-distance PCR (LD-PCR) technique. The titer of the primary cDNA library was 3.6 ×106 cfu・mL-1 and that of the amplified library was 1.2 × 1010 cfu・mL-1. Gel electrophoresis results showed that most of the cDNA inserts ranged from 0.40 to 2.5 kb, with a recombination rate of 99%. A total of 427 randomly selected positive clones were sequenced. After removing the unsuccessful reads, 364 datasets were obtained and have been submitted to the NCBI Nucleotide Sequence Database under GenBank accession numbers JK265131-JK265494. Among the 364 submitted sequences, 74.45% of them contained full-length coding regions. BLASTX analysis revealed that 62.36% of the ‘Torvum Vigor’ expressed sequence tags (ESTs) possessed homology to known or putative proteins of other organisms. Seven genes that might be responsible for the encoding of known proteins in other organisms were identified to confer salt tolerance. This evidence demonstrated that the cDNA library constructed was a full-length library of high quality. It could be a useful resource for further research in the cloning of stress-related genes, which could be utilized in the genetic improvement of vegetable crops using transgenic technology.
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