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RISS 인기검색어
- 검색키워드
- 저자 : Junyi Gai (검색결과 3 건)
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Jiajia Li,Shouping Yang,Junyi Gai 한국유전학회 2017 Genes & Genomics Vol.39 No.10
To further elucidate the molecular mechanism and fertility restoration of cytoplasmic male sterility (CMS) in soybean, a comparative transcriptome analysis was conducted between the CMS line NJCMS1A, restorer line NJCMS1C and their hybrid F1 progeny (NJCMS1A × NJCMS1C) using RNA-Seq strategy. After pairwise comparative analysis of these soybean lines, 294, 222, and 288 differentially expressed genes (DEGs) were identified, respectively. Further bioinformatic analysis indicated that these DEGs were involved in diverse molecular functions and metabolic pathways. qRT-PCR analysis validated that the gene expression pattern in RNA-Seq was reliable. These results significantly showed that the male sterility and fertility restoration in NJCMS1A might be related to a series of the abnormal of growth development and metabolic processes, such as pollen development, DNA methylation process, pollen viability, cell wall development, programmed cell death, as well as carbohydrate and energy metabolism. This study could facilitate our understanding of the molecular mechanisms and fertility restoration behind CMS in soybean.
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Mapping QTLs for Tissue Culture Response in Soybean (Glycine max (L.) Merr.)
Chao Yang,Tuanjie Zhao,Deyue Yu,Junyi Gai 한국분자세포생물학회 2011 Molecules and cells Vol.32 No.4
Quantitative trait loci (QTLs) that control the tissue cul-ture response in soybean were detected by using 184 recombinant inbred lines (RILs) derived from two varieties: Kefeng No.1 and Nannong 1138-2. The molecular map consisting of 834 molecular markers using this population covered space 2307.83 cM of the genome throughout 24 linkage groups. The performance of tissue culture in soybean was evaluated by two indices: callus induction frequency (CIF) and somatic embryos initiation frequency (SEIF). They were expressed as the number of explants producing callus/ the number of total explants and the number of explants producing somatic embryos/ the number of total explants, respectively. The RIL lines showed continuous segregation for both indices. With the composite interval mapping (CIM) described in Windows QTL Cartographer Version 2.5, three quantitative trait loci (QTLs) were identified for the frequency of callus induction, on chromosomes B2 and D2, accounting for phenotypic variation from 5.84% to 16.60%; four QTLs on chromo-some G were detected for the frequency of somatic em-bryos initiation and explained the phenotypic variation from 7.79% to 14.16%. The information of new QTLs identified in the present study will contribute to genetic improvement of regeneration traits with marker-assisted selection (MAS) in soybean.
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miR156b from Soybean CMS Line Modulates Floral Organ Development
Xianlong Ding,Hui Ruan,Lifeng Yu,Qiang Li,Qijian Song,Shouping Yang,Junyi Gai 한국식물학회 2020 Journal of Plant Biology Vol.63 No.2
The miR156 and plant specifc transcription factor SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) are known for their function regulating plant growth and development. In this study, we identifed 20 GmSPLs which are targeted by gma-miR156b via plant small RNA target and degradome analysis. And we found high transcript levels of gma-miR156b and its targeted GmSPLs in the fower of soybean cytoplasmic male sterility (CMS) line and its maintainer. The gma-miR156b direct cleavage of GmSPL2b and GmSPL9b, and have opposite expression levels during early fower buds development. We observed a high expression level of GUS protein in the anthers of the line with pgma-MIR156b::GUS reporter. Over-expression of the gma-miR156b precursor in Arabidopsis inhibited foral organ development, including reduced anther size and the amount of pollen grains per anther etc. Like miR156-targeted SPL genes, non-targeted GmSPL8s were also down-regulated in early fower bud development of soybean CMS line compared with its maintainer line, which might act in concert with miR156-targeted SPL genes to participate in the foral organ development. Quantitative real time PCR (qRT-PCR) suggested that miR156/SPL modulates foral organ development by regulating the expression of LATERAL ORGAN BOUNDARIES DOMAIN22 (LBD22), LBD36, AGAMOUS-LIKE30 (AGL30) and AGL104. Our fndings will facilitate understanding of the biological functions of miR156/SPL in foral organ development of soybean CMS.
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