Identification of Ellagitannins in Black Raspberry Seeds and Optimization of Accelerated Solvent Extraction of Ellagitannins Using Artificial Neural Network Coupled with Genetic Algorithm

Ga Eun Lee,Ryun Hee Kim,Taehwan Lim,Jaecheol Kim,Suna Kim,Keum Taek Hwang 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

This study aimed to identify ellagitannins in black raspberry (Rubus occidentalis) seeds (BS) and to optimize accelerated solvent extraction of ellagitannins using artificial neural network (ANN) coupled with genetic algorithm (GA). Ellagitannins were fractionated using Amberlite XAD-16N and Sephadex LH-20 resins. The ellagitannin fraction was qualitatively analyzed by HPLC-Q-TOF-MS/MS. BS had mainly monomeric and dimeric ellagitannins such as pedunculagin, castalagin, acutissimin A, sanguiin H-10, casuarictin, sanguiin H-2, sanguiin H-6, and sanguiin H-6 without a gallic moiety. For ANN modeling, extraction time (3-9 min), extraction temperature (40-70°C), and solvent concentration (50-100% acetone) were set as independent input variables, and total ellagitannin content was set as output variable. The ANN of this study had an input layer with three neurons, a hidden layer with ten neurons, and an output layer with one neuron. The optimal extraction conditions of the total ellagitannin content were predicted by the trained ANN coupled with GA. This study is the first attempt to analyze ellagitannin content in BS.

대구지역 야생조류로부터 분리한 Salmonella속 균의 생물화학적 특성과 Plasmid Profile

윤가리,이영주,김기석,탁연빈 한국수의공중보건학회 2003 예방수의학회지 Vol.27 No.2

The present study was conducted to investigate the prevalence, biochemical properties and plasmid profiles of Salmonella organisms isolated from wild birds in Daegu area during the period from May, 1999 to August, 2000. Twenty strains of Salmonella were identified from 192 samples of heron feces and 86 samples of pigeon feces. Among Salmonella isolates, S. montevideo were the most frequent serotype (16 strains), 3 strains of them were S. typhimurium and the other one was untypable. The biochemical properties of 20 strains of Salmonella isolated were in accordance with the traditional characteristics of Salmonella spp. in the majority of biochemical test such as IMViC, carbohydrate fermentation and amino acid decarboxylation. The majority of isolates were highly susceptible to amikacin, ampicillin, amoxicillin, carbenicillin, cephalothin, ciprofloxacine, chloramphenicol, enrofloxacin, furazolidone, gentamicin, kanamycin, nalidixic acid, polymixin B and sulfamethoxazole, while they were moderately susceptible to streptomycin and tetracyclin. However, the majority of them were highly resistant to nitrofurantoin. In plasmid profile, 21.1 kilobase (Kb), 20 Kb and 13.2 Kb fractions were common to three S. tvphimurium and one untypable strains, and no fraction was shown in all strains of S. montevideo.

A Study on the Anti-melanogenic and Anti-oxidant Activities of Fallopia sachalinensis Extract

Ga Ryun Kim,Chan Won Lee,Youn Kyoung Son,Hye Yoon Park 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

A Study on the Anti-pollutionary and Anti-oxidant Activities of Tilia Mandshurica Extracts

Ga Ryun Kim,Soo-Young Kim,Sarah Lee,Youn Kyoung Son,Hye Yoon Park 한국식품영양과학회 2019 한국식품영양과학회 학술대회발표집 Vol.2019 No.10

하악골의 유피낭종 : 증례 보고

Jung-Won Park,Ryun-Ga Kim,Sang-Hwa Roh,Tea-Young Jung,Sang-Jun Park 대한구강악안면병리학회 2015 대한구강악안면병리학회지 Vol.39 No.6

We report a rare case of intraosseous epidermoid cyst in mandibular body. A non symptomatic patient without trauma history had a cystic lesion on mandible close to the third molar. The lesion was enucleated while extracting of left lower third molar. The microscopic findings were consistent with an epidermoid cyst. Key words:Epidermoid Cyst, Intraosseous, Mandible

발효된 미강의 항산화 효과 및 미백 효과

채가연 ( Ga Yeon Chae ),권륜희 ( Ryun Hee Kwon ),장민우 ( Min Woo Jang ),김민정 ( Min Jung Kim ),하배진 ( Bae Jin Ha ) 대한화장품학회 2011 대한화장품학회지 Vol.37 No.2

이 연구에서는 천연 항산화소재와 미백소재 개발을 위하여 미강을 Bacillus subtilis에 접종하여 40 ℃에서 36h 동안 발효한 미강추출(RFB)과 80 % ethanol을 이용한 미강추출물(RB)을 사용하여 연구하였다. 추출물의 free radical 소거활성, superoxide radical 소거활성, hydroxyl radical 소거활성은 농도 100 μL/mL에서 가장 큰 활성을 나타내었고 리놀산을 이용한 지질과산화 억제 활성 또한 농도 100 μL/mL에서 가장 큰 활성을 나타내었고, RB에 비하여 RFB의 더 높은 항산화 능을 확인하였다. Mushroom tyrosinase의 활성 저해효과는 100 μL/mL 농도에서 mushroom tyrosinase의 활성을 80 % 저해하였으며 이는 vitamin C에 비해 더 높은 활성을 나타내었다. 그러므로 우리는 미강 발효추출물이 우수한 항산화와 미백 효능을 갖는 화장품 소재로서 개발 가능성이 클 것으로 기대된다. In this study, we investigated rice bran fermented by Bacillus subtilis (RFB) at 40 ℃ for 36 h to develop a new natural antioxidant and whitening agent for new natural cosmetics. RFB showed reactive oxygen species (ROS) scavenging activities in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, superoxide radical scavenging activities and hydroxyl radical scavenging activities at a concentration of 100 μL/mL. DPPH, superoxide radical scavenging activities and hydroxyl radical scavenging activities were higher in the RFB than in the RB. Inhibitory activity on auto-oxidation of linoleic acid is also highest at a concentration of 100 μL/mL. RFB showed the higher inhibitory activity than RB in auto-oxidation. RFB reduced intracellular tyrosinase activity about 80 % at a concentration of 100 μL/mL. Therefore, we suggest that RFB could be used as a useful antioxidant and whitening agent.

남녀별 직업군에 따른 대사증후군의 구성요소와 유병률 및 영양 상태의 비교 연구

김가람(Kim, Ga Ram),박혜련(Park, Hae Ryun),이영미(Lee, Young Mi),임영숙(Lim, Young Suk),송경희(Song, Kyung Hee) 한국영양학회 2017 Journal of Nutrition and Health Vol.50 No.1

본 연구에서는 2013년 국민건강영양조사 자료를 이용하여 30~64세 무직을 제외한 근로자 1,750명 (남성 892명, 여성 858명)을 대상으로 화이트칼라와 블루칼라로 직업군을 분류하였으며 이를 통해 직업군에 따른 대사증후군의 구성요소와 유병률 및 영양 상태를 비교 연구하였다. 그 결과 대사증후군 영향 요인으로 작용하는 사회경제적 지표인 교육수준과 소득수준에서 남녀 모두 화이트칼라가 블루칼라보다 높았다. 대사증후군 유병률을 살펴보았을 때, 남성의 경우 블루칼라 35.1%, 화이트칼라 26.8%로 블루칼라가 화이트칼라보다 높게 나타났으며, 여성의 경우 블루칼라 24.8%, 화이트칼라 8.9%로 블루칼라가 화이트칼라 보다 대사증후군 유병률이 높았다. 남녀를 비교하였을 때는 여성보다 남성에서 대사증후군 유병률이 높은 것으로 나타났다. 대사증후군 구성요소의 유병률에서는 남성의 경우 고혈당이 블루칼라가 화이트칼라보다 유의적으로 유병률이 높았으며 여성의 경우 복부 비만, 고혈압, 고혈당, 고중성지방혈증, 저HDL콜레스테롤혈증 모두 블루칼라가 화이트칼라보다 유의적으로 유병률이 높았다. 음식군별 1회 섭취량을 고려하여 주당 섭취빈도를 살펴본 결과 직업군별 남성의 경우 블루칼라가 화이트칼라보다 밥류의 주당 섭취빈도가 많았으며 빵·떡류의 주당 섭취빈도는 화이트칼라가 블루칼라보다 유의적으로 많이 섭취하였다. 여성의 경우는 블루칼라가 화이트칼라보다 국·찌개류의 주당 섭취빈도가 많았으며 과일류의 주당 섭취빈도는 화이트칼라가 블루칼라보다 유의적으로 많이 섭취하였다. 영양소 섭취량은 총 에너지 섭취량을 제외한 나머지 영양소 섭취량에서 남녀 모두 화이트칼라가 블루칼라보다 많이 섭취하였다. 따라서 본 연구 결과 남녀 모두 대사증후군 유병률은 블루칼라가 화이트칼라보다 높았으며 특히, 여성의 블루칼라에서 대사증후군과 그 구성요소의 유병률 비율이 높게 나타나 대사증후군 중재 및 예방을 위한 영양교육이 필요할 것이다. 또한, 남녀 모두 블루칼라가 화이트칼라보다 영양소 섭취 및 식사의 질이 전반적으로 저조한 것으로 나타나 블루칼라의 올바른 식습관 개선이 필요하다고 사료된다. 결론적으로 본 연구를 통해 성별에 따른 직업유형별 대사증후군 유병률과 영양소 섭취 상태가 상이하므로 이를 고려한 근로자들의 질병 예방을 위한 맞춤형 영양교육 서비스가 필요하다고 사료된다. Purpose: In this study, factors of metabolic syndrome and nutritional status were examined according to gender and occupations using the 2013 Korea National Health and Nutrition Examination Survey (KNHANES). Methods: This study was conducted on 1,750 workers (male : 892, female : 858) aged between 30 and 64, who participated in a health survey, health examination, and nutrition survey using the 6th 2013 KNHANES. Occupations were classified into white collar and blue collar workers, and nutrient intake was analyzed using a food frequency questionnaire. Analysis of complex sample design data through SPSS 19.0 was used for analysis. Results: The prevalence rate of metabolic syndrome among blue collar (35.1%) was higher than that among white collar workers (26.8%) in male subjects (p < 0.05) as well as in blue collar (24.8%) compared to white collar workers (8.9%) in female subjects (p < 0.001). Intake frequency per week, considering one portion by food category, showed significant differences in cooked rice (p < 0.05) and bakeries and confectioneries (p < 0.05) in make workers as well as stew and casserole (p < 0.01) and fruits (p < 0.05) in female workers. With regard to nutrient intake by occupation and gender, white collar workers consumed a greater amount of nutrients (not including total energy intake) compared to blue collar workers in both male and female workers. With regard to nutrient adequacy ratio (NAR) and mean adequacy ratio (MAR) according to gender and occupation, white collar workers showed higher numbers than blue collar workers in both male and female subjects. Conclusions: This study examined the prevalence rates of metabolic syndrome and nutrient intake according to gender and occupation. In both male and female subjects, blue collar workers showed higher prevalence rates compared to white collar workers, and their diet quality was worse than white collar workers" diet quality. Considering this result, customized nutrition education according to gender and occupation should be provided to workers to prevent diseases.

Systematic Investigation of the Reduction of Inorganic Arsenic and Bioactive Nutrients in Rice with Various Cooking Techniques

Yim, Sang-Ryun,Kim, Ji Hyun,Choi, Mi-Hee,Park, Ga Young,Shim, Soon-Mi,Chung, Myung-Sub ational Association for Food Protection 2017 Journal of food protection Vol.80 No.11

<B>ABSTRACT</B><P>The objectives of this study were to investigate the effect of polishing, stirring and rinsing, soaking, or pressure-steam cooking on total organic and inorganic arsenic content and to measure daily inorganic arsenic exposure and nutrient values from consumption of rice prepared under the optimal process. With increasing numbers of rinses and times stirred per rinse, the total arsenic content significantly decreased by 27.6% in brown rice and 39.0% in white rice with 10% degree of polishing (10DOP%). The increase in ratio of water to rice from 1.5:1 to 6:1 reduced total arsenic by 4.4 to 7.5%, depending on soaking time. The total arsenic concentrations found in samples prepared using the optimal process for cooked brown rice and for white rice with 5DOP%, 7DOP%, and 10DOP% were 56.1, 49.6, 52.0, and 42.0%, respectively, compared with those after bran residues were removed. The concentration of total dietary fiber in 10DOP% white and brown rice was 0.34 mg/100 g (54.7% reduction) and 2.52 mg/100 g (24.8% reduction) after the cooking process, respectively. Results from the current study suggest that the optimal cooking process could maximize the reduction of arsenic contents and, at the same time, minimize the loss of nutrients from rice.</P>

건축저작권 보호를 위한 입법적 정비방안

박가영(Park Ga young),김동련(Kim Dong ryun) 한국부동산법학회 2014 不動産法學 Vol.18 No.-

저작물이란 사상이나 감정을 문학, 학술, 예술 등으로 표현한 창작물이며, 우리 저작권법은 저작물을 크게 어문저작물, 음악저작물, 연극저작물, 미술저작물, 건축저작물, 사진저작물, 영상저작물, 도형저작물, 컴퓨터프로그램저작물의 9가지 유형으로 구분하고 있다. 그 중에서 건축저작물을 눈여겨 볼 수 있다. 건축저작물이란 건축에 관련된 저작물로서 일반적으로 건축물과 설계도면 및 모형을 지칭한다. 이 용어는 생소하지만 엄연히 저작권법에서 규정하고 있는 것으로서 다만 모든 건축물이나 설계도면 및 모형이 저작물로서 보호받지는 못한다. 본고에서는 저작권법에서 명문화하고 있는 내용을 바탕으로 하여 건축저작물에 대한 전박전인 기본 개념을 살펴보면서 건축저작권의 특성과 저작권에서 보호해주는 건축저작물의 한계를 지적하고 그에 따른 개선방안을 모색하고자 한다. 첫째, 건축저작권에 대한 창작성과 예술성에 대한 기준마련이다. 둘째, 건축저작물에 대한 범위 변경이 필요하다. 셋째, 건축저작권에 대한 강행규정을 통한 저작권자의 명확히 할 필요가 있다. 넷째, 건축저작권은 광범위 및 다양성을 가지고 있으므로 독립된 입법이 필요하다고 할 수 있다. An "Architectural works" is the design of a building as embodied in any tangible medium of expression, including a building, architectural plans, or drawings. Copyright in architecture is on of th grey areas in contemporary practices. Architectural Works, in general include buildings and their plans and models. however, not all the architectural works are protected by copyright laws as they should satisfy copyrightable attributcs. Though the term is unfamiliar to most of the people, it is provided by Copyright Act. However, this Act does not include all of the works -buildings, architectural plans, or drawings In this article, based on Copyright Act, we're going to examine the general meaning of an architectural work and the characteristics of an Architectural Works Copyright. Also, we will discuss the application limit of Copyright Act and how to improve it.

Anti-Pollutionary Activities of Native Plants, Carpinus tschonoskii and Euphorbia supina and Study on Synthetic Mechanism of Intracellular Ceramides

Hye Yoon Park(박혜윤),Ga Ryun Kim(김가륜) 한국약용작물학회 2019 한국약용작물학회 학술대회논문집 Vol.2019 No.1