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Three species of the family Noctuidae (Lepidoptera) new to China
Hui-Lin HAN,Zhao-Yao DUAN,Zhi-Wei FENG2 한국곤충학회 2008 Entomological Research Vol.38 No.3
Three species of the family Noctuidae –- Phlogophora striatovirens (Moore, 1867), Usbeca kulmburgi (Rebel, 1918) and Feliniopsis leucostigma (Moore, 1867) –- are reported for the first time from China. Photographs of adults and genital characteristics are provided.
Yang Hui-Hui,Jiang Hui-Ling,Tao Jia-Hao,Zhang Chen-Yu,Xiong Jian-Bing,Yang Jin-Tong,Liu Yu-Biao,Zhong Wen-Jing,Guan Xin-Xin,Duan Jia-Xi,Zhang Yan-Feng,Liu Shao-Kun,Jiang Jian-Xin,Zhou Yong,Guan Cha-Xi 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation of mitochondrial citrate (citratemt) in lipopolysaccharide (LPS)-treated AECs because of the downregulation of Idh3α and citrate carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition of Idh3α and Slc25a1 induced citratemt accumulation and necroptosis in vitro. Mice with AEC-specific Idh3α and Slc25a1 deficiency exhibited exacerbated lung injury and AEC necroptosis. Interestingly, the overexpression of Idh3α and Slc25a1 decreased citratemt levels and rescued AECs from necroptosis. Mechanistically, citratemt accumulation induced mitochondrial fission and excessive mitophagy in AECs. Furthermore, citratemt directly interacted with FUN14 domain-containing protein 1 (FUNDC1) and promoted the interaction of FUNDC1 with dynamin-related protein 1 (DRP1), leading to excessive mitophagy-mediated necroptosis and thereby initiating and promoting ALI. Importantly, necroptosis induced by citratemt accumulation was inhibited in FUNDC1-knockout AECs. We show that citratemt accumulation is a novel target for protection against ALI involving necroptosis.
( Hui Jing ),( You Jin Dai ),( Yuan Yuan Bian ),( Hui Li ),( Xiao Jin Cui ),( Xiao Jie Yu ),( Song You ),( Feng Qing Hu ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.7
Based on their respective antitumor and thrombolytic activities, the superantigen staphylococcal enterotoxin C2 (SEC2) and staphylokinase (Sak) were chosen for the construction of the novel chimeric proteins Sak-linker- SEC2 and SEC2-linker-Sak using a linker composed of nine Ala residues. Both chimeric proteins possessed nearly the same PBMC proliferation stimulating activity and antitumor activity as SEC2 and thrombolytic activity as Sak. Neither the SEC2 or Sak component of each chimeric protein affected the activity of the other component. The results presented in this study provide a possible strategy to prevent and cure tumor thrombus.
A novel M2e-multiple antigenic peptide providing heterologous protection in mice
Feng Wen,Ji-Hong Ma,Hai Yu,Fu-Ru Yang,Meng Huang,Yan-Jun Zhou,Ze-Jun Li,Xiu-Hui Wang,Guo-Xin Li,Yi-Feng Jiang,Wu Tong,Guangzhi Tong 대한수의학회 2016 Journal of Veterinary Science Vol.17 No.1
Swine influenza viruses (SwIVs) cause considerable morbidity and mortality in domestic pigs, resulting in a significant economic burden. Moreover, pigs have been considered to be a possible mixing vessel in which novel strains loom. Here, we developed and evaluated a novel M2e-multiple antigenic peptide (M2e-MAP) as a supplemental antigen for inactivated H3N2 vaccine to provide cross-protection against two main subtypes of SwIVs, H1N1 and H3N2. The novel tetra-branched MAP was constructed by fusing four copies of M2e to one copy of foreign T helper cell epitopes. A high-yield reassortant H3N2 virus was generated by plasmid based reverse genetics. The efficacy of the novel H3N2 inactivated vaccines with or without M2e-MAP supplementation was evaluated in a mouse model. M2e-MAP conjugated vaccine induced strong antibody responses in mice. Complete protection against the heterologous swine H1N1 virus was observed in mice vaccinated with M2e-MAP combined vaccine. Moreover, this novel peptide confers protection against lethal challenge of A/Puerto Rico/8/34 (H1N1). Taken together, our results suggest the combined immunization of reassortant inactivated H3N2 vaccine and the novel M2e-MAP provided cross-protection against swine and human viruses and may serve as a promising approach for influenza vaccine development.
Hui Ni,Su Fang Zhang,Qiu Feng Gao,Yang Hu,Ze Dong Jiang,Feng Chen 한국식품과학회 2015 Food Science and Biotechnology Vol.24 No.4
Quantification of limonin, naringin, prunin, and naringenin is an important approach for monitoring of debittering processes of citrus products. Naringin and limonin have different polarity and solubility values, causing difficulty in simultaneous extraction and quantification. A procedure combining HPLC and solid-phase extraction was developed to simultaneously quantify these analytes in citrus juice. Analytes exhibited calibration curves of good linearity along with low limit of detection and limit of quantification values. Naringin, prunin, naringenin, and limonin exhibited respective recovery values of 92.2-100.6, 92.0-97.3, 98.1-102.2, and 102.4-103.9%, respectively. Relative standard deviations were lower than 5%. For analysis of naringin, prunin, naringenin, and limonin in citrus juices, the simultaneous method displayed analytical results identical to traditional respective quantification methods. The simultaneous method is highly effective for monitoring naringin, prunin, naringenin, and limonin levels in citrus juice.
Hui Ni,Yuan Fan Yang,Feng Chen,Hai Feng Ji,Hu Yang,Wu Ling,Hui Nong Cai 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.3
Pectinase and naringinase were investigated forimproving the production of pummelo juice by increasingthe juice yield and eliminating juice bitterness. Comparedto a control, the enzymatic treatment significantly (p<0.05)increased the juice yield, soluble pectin and total solublesolid (TSS) contents, and the clarity, while decreasing theconcentrations of the bitter chemicals naringin, limonin,and nomilin. A combined processing treatment of peelingand enzymatic hydrolyses using 5U/g of pectinase and 0.4 U/gof naringinase at 50oC for 60 min resulted in a juice yieldof 42.3%, a TSS content of 11.4oBx, a titrable acidity (TA)of 0.96%, a vitamin C concentration of 38.4 mg/100 mL,and concentrations of naringin, limonin, and nomilin of42.4 μg/mL, 33.5 μg/mL, and 13.3 μg/mL, respectively. The enzymatic method was effective and practical forproduction of high quantity pummelo juice.
Detection of HF-ERW Status by Neural Network on Imaging
Hui-Feng Wang,Jing Cao,Xiangmo Zhao,Xiao-Meng Wang,Gui-Ping Wang 한국정밀공학회 2017 International Journal of Precision Engineering and Vol.18 No.7
To achieve online testing of high-frequency electric resistance welding (HF-ERW) tube quality, forecasting models were established for welding defect conditions with collected high-speed images of the joint melting phenomenon, based on a radial basis function neural network (RBFNN). Firstly, the dimensions of the collected image samples were deduced by principal component analysis (PCA). Then, the reduced-dimension image samples were set as inputs of both BPNN (back-propagation neural network) and, for comparison, RBFNN, which were trained so that the model parameters were optimized. Finally, the testing sample set was identified by trained networks. The experimental results show that RBFNN had better generalization ability for HF-ERW images than BPNN, which meant that the recognition rate of low-heat input status reached 100%, while the recognition rate of overheating input status reached 97.67%. They also show that the welding quality detection system based on a neural network is very effective and has a strong guiding significance for welding quality control.