Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

R. F. Xu,K. Li,G. H. Chen,B. Y. Z. Qiang,D. L. Mo,B. Fan,C. C. Li,M. Yu,M. J. Zhu,T. A. Xiong,B. Liu 아세아·태평양축산학회 2005 Animal Bioscience Vol.18 No.7

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a nonsynonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variableregion- like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

Buckling of axial compressed cylindrical shells with stepwise variable thickness

Fan, H.G.,Chen, Z.P.,Feng, W.Z.,Zhou, F.,Shen, X.L.,Cao, G.W. Techno-Press 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.54 No.1

This paper focuses on an analytical research on the critical buckling load of cylindrical shells with stepwise variable wall thickness under axial compression. An arctan function is established to describe the thickness variation along the axial direction of this kind of cylindrical shells accurately. By using the methods of separation of variables, small parameter perturbation and Fourier series expansion, analytical formulas of the critical buckling load of cylindrical shells with arbitrary axisymmetric thickness variation under axial compression are derived. The analysis is based on the thin shell theory. Analytic results show that the critical buckling load of the uniform shell with constant thickness obtained from this paper is identical with the classical solution. Two important cases of thickness variation pattern are also investigated with these analytical formulas and the results coincide well with those obtained from other authors. The cylindrical shells with stepwise variable wall thickness, which are widely used in actual engineering, are studied by this method and the analytical formulas of critical buckling load under axial compression are obtained. Furthermore, an example is presented to illustrate the effects of each strake's length and thickness on the critical buckling load.

Intramuscular Administration of Zinc Metallothionein to Preslaughter Stressed Pigs Improves Anti-oxidative Status and Pork Quality

Li, L.L.,Hou, Z.P.,Yin, Y.L.,Liu, Y.H.,Hou, D.X.,Zhang, B.,Wu, G.Y.,Kim, S.W.,Fan, M.Z.,Yang, C.B.,Kong, X.F.,Tang, Z.R.,Peng, H.Z.,Deng, D.,Deng, Z.Y.,Xie, M.Y.,Xiong, H.,Kang, P.,Wang, S.X. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5

This study was conducted to determine the effects of exogenous zinc-metallothionein (Zn-MT) on anti-oxidative function and pork quality. After feeding a corn-soybean meal-based diet for two weeks, 48 pigs ($Duroc{\times}Landrace{\times}Chinese\;Black Pig$) were assigned randomly to four groups. Pigs in Group 1 were maintained under non-stress conditions, whereas pigs in Groups 2, 3 and 4 were aggressively handled for 25 min to produce stress. Pigs in Groups 1, 2, 3, and 4 received intramuscular administration of saline (control group; CON), 0 (negative control group; NCON), 0.8 (low dose group; LOW), and 1.6 (high dose group; HIGH) mg rabbit liver Zn-MT per kg body weight, respectively. Pigs were slaughtered at 3 and 6 h post-injection. Zn-MT treatment increased (p<0.05) the activities of superoxide dismutase (SOD) and glutathione-peroxidase (GSH-PX) while decreasing the concentration of malondialdehyde (MDA) in liver. These responses were greater (p<0.05) at 6 h than at 3 h post Zn-MT injection. Zn-MT treatment increased (p<0.05) hepatic SOD mRNA levels in a time and dose-dependent manner and decreased (p<0.05) serum glutamate-pyruvate transaminase and lactate dehydrogenase activities (indicators of tissue integrity). Zn-MT administration decreased (p<0.05) lactate concentration and increased (p<0.05) pH and water-holding capacity in the longissimus thorasis meat. Collectively, our results indicate that intramuscular administration of Zn-MT to pre-slaughter stressed pigs improved tissue anti-oxidative ability and meat quality.

Buckling of axial compressed cylindrical shells with stepwise variable thickness

H.G. Fan,Z.P. Chen,W.Z. Feng,F. Zhou,X.L. Shen,G.W. Cao 국제구조공학회 2015 Structural Engineering and Mechanics, An Int'l Jou Vol.54 No.1

This paper focuses on an analytical research on the critical buckling load of cylindrical shells with stepwise variable wall thickness under axial compression. An arctan function is established to describe the thickness variation along the axial direction of this kind of cylindrical shells accurately. By using the methods of separation of variables, small parameter perturbation and Fourier series expansion, analytical formulas of the critical buckling load of cylindrical shells with arbitrary axisymmetric thickness variation under axial compression are derived. The analysis is based on the thin shell theory. Analytic results show that the critical buckling load of the uniform shell with constant thickness obtained from this paper is identical with the classical solution. Two important cases of thickness variation pattern are also investigated with these analytical formulas and the results coincide well with those obtained from other authors. The cylindrical shells with stepwise variable wall thickness, which are widely used in actual engineering, are studied by this method and the analytical formulas of critical buckling load under axial compression are obtained. Furthermore, an example is presented to illustrate the effects of each strake’s length and thickness on the critical buckling load.

Genetic Variation and Genetic Relationship of Seventeen Chinese Indigenous Pig Breeds Using Ten Serum Protein Loci

Mo, D.L.,Liu, B.,Wang, Z.G.,Zhao, S.H.,Yu, M.,Fan, B.,Li, M.H.,Yang, S.L.,Zhang, G.X.,Xiong, T.A.,Li, K. Asian Australasian Association of Animal Productio 2003 Animal Bioscience Vol.16 No.7

Seventeen Chinese indigenous pig breeds and three introduced pig breeds had been carried out by means of vertical polyacrylamide gel electrophoresis (PAGE). According to the results, eight serum protein loci were highly polymorphic except Pi-2 and Cp. The polymorphism information content (PIC) of Hpx was the highest (0.5268), while that of Cp was the lowest (0.0257). The population genetic variation index showed that about 84% genetic variation existed in the population, and the rest of 16% distributed between the populations. The genetic variation of Yimeng black pig and Duroc were the highest and the lowest, respectively. The genetic variation of Chinese indigenous pig breeds was much more than that of exotic groups. Genetic distance results showed that Chinese indigenous pig breeds were classified into four groups with the three introduced pig breeds clustered into another group. The results also supported the geographic distribution of Chinese indigenous pig breeds in certain extent.

Optimal Bit Allocation Adaptive Modulation Algorithm for MIMO System

L. Fan,C. He,G. Feng 한국통신학회 2007 Journal of communications and networks Vol.9 No.2

Management of complex surgical wounds of the back: identifying an evidence-based approach

Elizabeth G. Zolper,Meher A. Saleem,Kevin G. Kim,Mark D. Mishu,Sarah R. Sher,Christopher E. Attinger,Kenneth L. Fan,Karen K. Evans 대한성형외과학회 2021 Archives of Plastic Surgery Vol.48 No.6

Background Postoperative dehiscence and surgical site infection after spinal surgery can carry serious morbidity. Multidisciplinary involvement of plastic surgery is essential to minimizing morbidity and achieving definitive closure. However, a standardized approach is lacking. The aim of this study was to identify effective reconstructive interventions for the basis of an evidence-based management protocol. Methods A retrospective review was performed at a single tertiary institution for 45 patients who required 53 reconstruction procedures with plastic surgery for wounds secondary to spinal surgery from 2010 to 2019. Statistical analysis was performed for demographics, comorbidities, and treatment methods. Primary outcomes were postoperative complications, including dehiscence, seroma, and infection. The secondary outcome was time to healing. Results The overall complication rate was 32%, with dehiscence occurring in 17%, seroma in 15% and infection in 11% of cases. Median follow-up was 10 months (interquartile range, 4–23). Use of antibiotic beads did not affect rate of infection occurrence after wound closure (P=0.146). Use of incisional negative pressure wound therapy (iNPWT) was significant for reduced time to healing (P=0.001). Patients treated without iNPWT healed at median of 67.5 days while the patients who received iNPWT healed in 33 days. Demographics and comorbidities between these two groups were similar. Conclusions This data provides groundwork for an evidence-based approach to soft tissue reconstruction and management of dehiscence after spinal surgery. Timely involvement of plastic surgery in high-risk patients and utilization of evidence-based interventions such as iNPWT are essential for improving outcomes in this population.

ELECTROMAGNETIC VALVE TRAIN FOR GASOLINE ENGINE EXHAUST SYSTEM

X. Y. FAN,L. LIU,S. Q. CHANG,J. T. XU,J. G. DAI 한국자동차공학회 2016 International journal of automotive technology Vol.17 No.3

Electromagnetic valve train (EMVT) in camless engine offers large potential for both part load fuel economy and high load engine torque. However, it is more difficult to be applied on exhaust system than intake system. Because the gas pressure brings high demands for driving force, especially at high engine speed and full load. Based on the working characters of actuator, a method by increasing the transient currents in windings during valve’s opening motion is suggested to overcome the gas pressure. But this will cause more energy losses and heat. In order to make the EMVT used on exhaust system better, quantitative analysis is carried out against the additional power consumption caused by gas pressure under different conditions. Furthermore, an approach is introduced to define the optimal exhaust valve opening motion at full load conditions. It aims at making a better compromise between the engine power output and exhaust valves’ power consumption, thus both the efficiency of EMVT and engine performance are enhanced.

Effect of channel thickness on electrical performance of amorphous IGZO thin-film transistor with atomic layer deposited alumina oxide dielectric

Y. Li,Y.L. Pei,R.Q. Hu,Z.M. Chen,Y. Zhao,Z. Shen,B.F. Fan,J. Liang,G.Wang 한국물리학회 2014 Current Applied Physics Vol.14 No.7

We have investigated the electrical performance of amorphous indiumegalliumezinc oxide (a-IGZO) thin-film transistors with various channel thicknesses. It is observed that when the a-IGZO thickness increases, the threshold voltage decreases as reported at other researches. The intrinsic field-effect mobility as high as 11.1 cm2/Vs and sub threshold slope as low as w0.2 V/decade are independent on the thickness of a-IGZO channel, which indicate the excellent interface between a-IGZO and atomic layer deposited Al2O3 dielectric even for the case with a-IGZO thickness as thin as 10 nm. However, the source and drain series resistances increased with increasing of a-IGZO channel thickness, which results in the apparent field-effect mobility decreasing. The threshold voltage shift (DVth) under negative bias stress (NBS) and negative bias illumination stress (NBIS) were investigated, also. The hump-effect in the sub threshold region under NBS and threshold voltage shift to negative position under NBIS were enhanced with decreasing of a-IGZO channel thickness, owing to the enhancement of vertical electrical field in channel.

Cutaneous mucormycosis of the lower extremity leading amputation in two diabetic patients

Coerdt, Kathleen M.,Zolper, Elizabeth G.,Starr, Amy G.,Fan, Kenneth L.,Attinger, Christopher E.,Evans, Karen K. Korean Society of Plastic and Reconstructive Surge 2021 Archives of Plastic Surgery Vol.48 No.2

Mucormycosis is an invasive, rapidly progressive, life-threatening fungal infection, with a propensity for diabetic, immunosuppressed, and trauma patients. The classic rhinocerebral variation is most common in diabetic patients. While the cutaneous form is usually caused by direct inoculation in immunocompetent patients. Cutaneous mucormycosis manifests in soft tissue and risks involvement of underlying structures. Tibial osteomyelitis can also occur secondary to cutaneous mucormycosis but is rare. Limb salvage is typically successful after lower extremity cutaneous mucormycosis even when the bone is involved. Herein, we report two cases of lower extremity cutaneous mucormycosis in diabetic patients that presented as acute worsening of chronic pretibial ulcers. Despite aggressive antifungal therapy and surgical debridement, both ultimately required amputation. Such aggressive presentation has not been reported in the absence of major penetrating trauma, recent surgery, or burns.